Restriction map for
pF1KB7057
(Circular) (Six-base) MAPSORT of: pF1KB7057.seq Check: 8861 from: 1 to: 4928
pF1KB7057 4928 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 1236 1460 494
Size: 742 224 3962
Fragments arranged by size:
3962 742 224
AatII G_ACGT'C
Cuts at: 1574 1574
Size: 4928
AccI GT'mk_AC
Cuts at: 1966 1966
Size: 4928
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4928
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 922 2753 3185 3932 4200 732
Size: 190 1831 432 747 268 1460
Fragments arranged by size:
1831 1460 747 432 268 190
AflIII A'CryG_T
Cuts at: 815 959 2280 3384 4361 815
Size: 144 1321 1104 977 1382
Fragments arranged by size:
1382 1321 1104 977 144
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AleI CACnn'nnGTG
Cuts at: 1195 1195
Size: 4928
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 946 978 1234 1266 946
Size: 32 256 32 4608
Fragments arranged by size:
4608 256 32 32
AlwNI CAG_nnn'CTG
Cuts at: 459 623 1395 1476 2233 3800 459
Size: 164 772 81 757 1567 1587
Fragments arranged by size:
1587 1567 772 757 164 81
ApaI G_GGCC'C
Cuts at: 172 223 1546 172
Size: 51 1323 3554
Fragments arranged by size:
3554 1323 51
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 1932 4281 4537 1932
Size: 2349 256 2323
Fragments arranged by size:
2349 2323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 324 882 1335 1683 1867 1948 324
Size: 558 453 348 184 81 3304
Fragments arranged by size:
3304 558 453 348 184 81
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 331 886 1614 1944 2358 2606 2641 331
Size: 555 728 330 414 248 35 2618
Fragments arranged by size:
2618 728 555 414 330 248 35
BanII G_rGCy'C
Cuts at: 172 223 275 602 767 1343 1424 1546 1883
Size: 51 52 327 165 576 81 122 337
Cuts at: 1883 1942 2972 172
Size: 59 1030 2128
Fragments arranged by size:
2128 1030 576 337 327 165 122 81
59 52 51
BbeI G_GCGC'C
Cuts at: 890 2362 2610 890
Size: 1472 248 3208
Fragments arranged by size:
3208 1472 248
BbsI GAAGACnn'nnnn_
Cuts at: 273 273
Size: 4928
BbvCI CC'TCA_GC
Cuts at: 565 1639 1897 565
Size: 1074 258 3596
Fragments arranged by size:
3596 1074 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 849 883 849
Size: 34 4894
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3587 4928 2826
Size: 761 1341 2826
Fragments arranged by size:
2826 1341 761
BclI T'GATC_A
Cuts at: 2448 2448
Size: 4928
BglI GCCn_nnn'nGGC
Cuts at: 437 808 1376 437
Size: 371 568 3989
Fragments arranged by size:
3989 568 371
BglII A'GATC_T
Cuts at: 2443 4480 2443
Size: 2037 2891
BlpI GC'TnA_GC
Cuts at: 274 1097 2040 274
Size: 823 943 3162
Fragments arranged by size:
3162 943 823
Bme1580I G_kGCm'C
Cuts at: 172 223 232 1546 1784 2553 2646 3702 172
Size: 51 9 1314 238 769 93 1056 1398
Fragments arranged by size:
1398 1314 1056 769 238 93 51 9
BmrI ACTGGGnnnn_n'
Cuts at: 175 1630 1766 2140 2221 2556 4700 175
Size: 1455 136 374 81 335 2144 403
Fragments arranged by size:
2144 1455 403 374 335 136 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 276 384 466 514 610 823 973 1075
Size: 74 108 82 48 96 213 150 102
Cuts at: 1075 1141 1306 1438 1507 1609 1711 1726 202
Size: 66 165 132 69 102 102 15 3404
Fragments arranged by size:
3404 213 165 150 132 108 102 102
102 96 82 74 69 66 48 15
Bpu10I CC'TnA_GC
Cuts at: 565 781 1639 1897 565
Size: 216 858 258 3596
Fragments arranged by size:
3596 858 258 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1342 1723 2104 2983 3475 3773 1147
Size: 195 381 381 879 492 298 2302
Fragments arranged by size:
2302 879 492 381 381 298 195
BsaI GGTCTCn'nnnn_
Cuts at: 4632 4632
Size: 4928
BsaAI yAC'GTr
Cuts at: 2281 2281
Size: 4928
BsaBI GATnn'nnATC
Cuts at: 6 2442 4479 6
Size: 2436 2037 455
Fragments arranged by size:
2436 2037 455
BsaHI Gr'CG_yC
Cuts at: 887 1535 1571 2359 2607 3309 4805 887
Size: 648 36 788 248 702 1496 1010
Fragments arranged by size:
1496 1010 788 702 648 248 36
BsaWI w'CCGG_w
Cuts at: 1229 1239 2117 2638 3316 3590 3737 4148 1229
Size: 10 878 521 678 274 147 411 2009
Fragments arranged by size:
2009 878 678 521 411 274 147 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 946 976 1234 1264 1417 1447 1684 1714 946
Size: 30 258 30 153 30 237 30 4160
Fragments arranged by size:
4160 258 237 153 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1072 1399 1072
Size: 327 4601
BseYI C'CCAG_C
Cuts at: 222 435 462 975 1068 2353 3111 3688 4184
Size: 213 27 513 93 1285 758 577 496
Cuts at: 4184 222
Size: 966
Fragments arranged by size:
1285 966 758 577 513 496 213 93
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 844 1030 1255 1264 472
Size: 372 186 225 9 4136
Fragments arranged by size:
4136 372 225 186 9
BsiEI CG_ry'CG
Cuts at: 104 236 2470 2516 3724 104
Size: 132 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 132 46
BsiHKAI G_wGCw'C
Cuts at: 275 662 767 1136 1424 1942 2720 2910 3702
Size: 387 105 369 288 518 778 190 792
Cuts at: 3702 275
Size: 1501
Fragments arranged by size:
1501 792 778 518 387 369 288 190
105
BsmBI CGTCTCn'nnnn_
Cuts at: 1578 1578
Size: 4928
Bsp1286I G_dGCh'C
Cuts at: 172 223 232 275 602 662 767 1136 1343
Size: 51 9 43 327 60 105 369 207
Cuts at: 1343 1424 1546 1784 1883 1942 2553 2646 2720
Size: 81 122 238 99 59 611 93 74
Cuts at: 2720 2910 2972 3702 172
Size: 190 62 730 1398
Fragments arranged by size:
1398 730 611 369 327 238 207 190
122 105 99 93 81 74 62 60
59 51 43 9
BspEI T'CCGG_A
Cuts at: 1229 1239 4148 1229
Size: 10 2909 2009
Fragments arranged by size:
2909 2009 10
BspHI T'CATG_A
Cuts at: 1205 4104 1205
Size: 2899 2029
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 494 740 1052 1236 1460 1502 1895 1978
Size: 66 246 312 184 224 42 393 83
Cuts at: 1978 2192 2494 2875 428
Size: 214 302 381 2481
Fragments arranged by size:
2481 393 381 312 302 246 224 214
184 83 66 42
BsrBI CCG'CTC
Cuts at: 50 498 3220 3274 4758 4923 50
Size: 448 2722 54 1484 165 55
Fragments arranged by size:
2722 1484 448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1594 2840 1594
Size: 1246 3682
BsrFI r'CCGG_y
Cuts at: 1327 1527 2926 3107 3316 1327
Size: 200 1399 181 209 2939
Fragments arranged by size:
2939 1399 209 200 181
BssSI C'ACGA_G
Cuts at: 1372 3199 3557 1372
Size: 1827 358 2743
Fragments arranged by size:
2743 1827 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 2305 1278
Size: 1027 3901
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstEII G'GTnAC_C
Cuts at: 1711 1711
Size: 4928
BstYI r'GATC_y
Cuts at: 1 1953 2420 2443 2778 3024 4036 4122 4134
Size: 1952 467 23 335 246 1012 86 12
Cuts at: 4134 4480 1
Size: 346 449
Fragments arranged by size:
1952 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 115 1645 115
Size: 1530 3398
BtgI C'CryG_G
Cuts at: 107 678 875 107
Size: 571 197 4160
Fragments arranged by size:
4160 571 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 852 927 996 2878 3059 3222 852
Size: 75 69 1882 181 163 2558
Fragments arranged by size:
2558 1882 181 163 75 69
BtsI GCAGTG_nn'
Cuts at: 1657 2171 1657
Size: 514 4414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 325 360 325
Size: 35 4893
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3492 2634
Size: 858 4070
EaeI y'GGCC_r
Cuts at: 233 429 758 2513 2687 3078 3105 4450 233
Size: 196 329 1755 174 391 27 1345 711
Fragments arranged by size:
1755 1345 711 391 329 196 174 27
EagI C'GGCC_G
Cuts at: 233 2513 233
Size: 2280 2648
EarI CTCTTCn'nnn_
Cuts at: 607 751 1357 1471 2951 3161 607
Size: 144 606 114 1480 210 2374
Fragments arranged by size:
2374 1480 606 210 144 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1300 1333 1423 1546 3136 3446 3592 4738 1300
Size: 33 90 123 1590 310 146 1146 1490
Fragments arranged by size:
1590 1490 1146 310 146 123 90 33
EcoICRI GAG'CTC
Cuts at: 273 765 1422 1940 273
Size: 492 657 518 3261
Fragments arranged by size:
3261 657 518 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 276 384 466 514 610 732 823 922
Size: 74 108 82 48 96 122 91 99
Cuts at: 922 973 1075 1141 1306 1438 1507 1609 1711
Size: 51 102 66 165 132 69 102 102
Cuts at: 1711 1726 2753 3185 3932 4200 202
Size: 15 1027 432 747 268 930
Fragments arranged by size:
1027 930 747 432 268 165 132 122
108 102 102 102 99 96 91 82
74 69 66 51 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 952 1468 1900 952
Size: 516 432 3980
Fragments arranged by size:
3980 516 432
EcoO109I rG'GnC_Cy
Cuts at: 169 219 609 627 1041 1113 1257 1473 1543
Size: 50 390 18 414 72 144 216 70
Cuts at: 1543 1893 2067 169
Size: 350 174 3030
Fragments arranged by size:
3030 414 390 350 216 174 144 72
70 50 18
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4928
FspI TGC'GCA
Cuts at: 511 2709 511
Size: 2198 2730
HaeII r_GCGC'y
Cuts at: 142 460 890 910 1178 1698 1747 2362 2610
Size: 318 430 20 268 520 49 615 248
Cuts at: 2610 3632 4611 142
Size: 1022 979 459
Fragments arranged by size:
1022 979 615 520 459 430 318 268
248 49 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1340 1372 1417 1449 2770 2800 2802 2832 1340
Size: 32 45 32 1321 30 2 30 3436
Fragments arranged by size:
3436 1321 45 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1967 1967
Size: 4928
KasI G'GCGC_C
Cuts at: 886 2358 2606 886
Size: 1472 248 3208
Fragments arranged by size:
3208 1472 248
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4928
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1460 3599 3783 1460
Size: 2139 184 2605
Fragments arranged by size:
2605 2139 184
MscI TGG'CCA
Cuts at: 2689 2689
Size: 4928
MslI CAynn'nnrTG
Cuts at: 1195 2917 3044 1195
Size: 1722 127 3079
Fragments arranged by size:
3079 1722 127
MspA1I CmG'CkG
Cuts at: 435 507 680 752 828 1068 1092 1295 1554
Size: 72 173 72 76 240 24 203 259
Cuts at: 1554 2039 2353 2713 3726 3971 435
Size: 485 314 360 1013 245 1392
Fragments arranged by size:
1392 1013 485 360 314 259 245 240
203 173 76 72 72 24
NaeI GCC'GGC
Cuts at: 1529 3109 1529
Size: 1580 3348
NarI GG'CG_CC
Cuts at: 887 2359 2607 887
Size: 1472 248 3208
Fragments arranged by size:
3208 1472 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4928
NgoMIV G'CCGG_C
Cuts at: 1527 3107 1527
Size: 1580 3348
NspI r_CATG'y
Cuts at: 1981 2481 3388 1981
Size: 500 907 3521
Fragments arranged by size:
3521 907 500
PasI CC'CwG_GG
Cuts at: 1539 1718 1539
Size: 179 4749
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4928
PflMI CCAn_nnn'nTGG
Cuts at: 1476 1476
Size: 4928
PfoI T'CCnGG_A
Cuts at: 292 767 950 1052 1157 292
Size: 475 183 102 105 4063
Fragments arranged by size:
4063 475 183 105 102
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 946 978 1234 1266 4067 4099 946
Size: 32 256 32 2801 32 1775
Fragments arranged by size:
2801 1775 256 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 627 1041 1893 627
Size: 414 852 3662
Fragments arranged by size:
3662 852 414
PspOMI G'GGCC_C
Cuts at: 168 219 1542 168
Size: 51 1323 3554
Fragments arranged by size:
3554 1323 51
PstI C_TGCA'G
Cuts at: 250 289 425 737 1067 1553 1766 1975 2660
Size: 39 136 312 330 486 213 209 685
Cuts at: 2660 250
Size: 2518
Fragments arranged by size:
2518 685 486 330 312 213 209 136
39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 435 507 828 1068 1092 1295 2353 2713 435
Size: 72 321 240 24 203 1058 360 2650
Fragments arranged by size:
2650 1058 360 321 240 203 72 24
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 275 767 1424 1942 275
Size: 492 657 518 3261
Fragments arranged by size:
3261 657 518 492
SacII CC_GC'GG
Cuts at: 681 681
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 607 751 2951 3161 607
Size: 144 2200 210 2374
Fragments arranged by size:
2374 2200 210 144
SbfI CC_TGCA'GG
Cuts at: 425 1975 425
Size: 1550 3378
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SfcI C'TryA_G
Cuts at: 33 246 285 421 733 1063 1549 1762 1971
Size: 213 39 136 312 330 486 213 209
Cuts at: 1971 2656 3649 3840 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 486 330 312 213 213
209 191 136 39
SfoI GGC'GCC
Cuts at: 888 2360 2608 888
Size: 1472 248 3208
Fragments arranged by size:
3208 1472 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 326 884 1685 1950 326
Size: 558 801 265 3304
Fragments arranged by size:
3304 801 558 265
SmlI C'TyrA_G
Cuts at: 1126 1357 1738 2083 2998 3490 3752 1126
Size: 231 381 345 915 492 262 2302
Fragments arranged by size:
2302 915 492 381 345 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4428
StuI AGG'CCT
Cuts at: 128 128
Size: 4928
StyI C'CwwG_G
Cuts at: 107 2062 2416 107
Size: 1955 354 2619
Fragments arranged by size:
2619 1955 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 147 349 2511 2644 3103 3312 147
Size: 202 2162 133 459 209 1763
Fragments arranged by size:
2162 1763 459 209 202 133
TatI w'GTAC_w
Cuts at: 4443 4443
Size: 4928
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1684 1716 4445 4477 1684
Size: 32 2729 32 2135
Fragments arranged by size:
2729 2135 32 32
Tth111I GACn'n_nGTC
Cuts at: 319 2725 319
Size: 2406 2522
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1608 2423 1608
Size: 815 4113
XmaI C'CCGG_G
Cuts at: 324 882 1683 1948 324
Size: 558 801 265 3304
Fragments arranged by size:
3304 801 558 265
ZraI GAC'GTC
Cuts at: 1572 1572
Size: 4928
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI