Restriction map for
pF1KB7058
(Circular) (Six-base) MAPSORT of: pF1KB7058.seq Check: 6764 from: 1 to: 4952
pF1KB7058 4952 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1495 1495
Size: 4952
AccI GT'mk_AC
Cuts at: 1990 1990
Size: 4952
Acc65I G'GTAC_C
Cuts at: 1968 1968
Size: 4952
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 868 1758 2777 3209 3956 4224 529
Size: 339 890 1019 432 747 268 1257
Fragments arranged by size:
1257 1019 890 747 432 339 268
AflIII A'CryG_T
Cuts at: 2304 3408 4385 2304
Size: 1104 977 2871
Fragments arranged by size:
2871 1104 977
AgeI A'CCGG_T
Cuts at: 3340 3340
Size: 4952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1132 1377 1132
Size: 245 4707
AlwNI CAG_nnn'CTG
Cuts at: 2257 3824 2257
Size: 1567 3385
ApaLI G'TGCA_C
Cuts at: 239 3722 239
Size: 3483 1469
ApoI r'AATT_y
Cuts at: 288 1956 4305 4561 288
Size: 1668 2349 256 679
Fragments arranged by size:
2349 1668 679 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 117 190 314 381 435 482 750 1538 1972
Size: 73 124 67 54 47 268 788 434
Cuts at: 1972 117
Size: 3097
Fragments arranged by size:
3097 788 434 268 124 73 67 54
47
BamHI G'GATC_C
Cuts at: 775 1977 775
Size: 1202 3750
BanI G'GyrC_C
Cuts at: 1968 2382 2630 2665 1968
Size: 414 248 35 4255
Fragments arranged by size:
4255 414 248 35
BanII G_rGCy'C
Cuts at: 117 315 1278 1350 1966 2996 117
Size: 198 963 72 616 1030 2073
Fragments arranged by size:
2073 1030 963 616 198 72
BbeI G_GCGC'C
Cuts at: 2386 2634 2386
Size: 248 4704
BbsI GAAGACnn'nnnn_
Cuts at: 625 1823 625
Size: 1198 3754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4918
BciVI GTATCCnnnnn_n'
Cuts at: 2850 3611 4952 2850
Size: 761 1341 2850
Fragments arranged by size:
2850 1341 761
BclI T'GATC_A
Cuts at: 2472 2472
Size: 4952
BfrBI ATG'CAT
Cuts at: 1820 1820
Size: 4952
BglI GCCn_nnn'nGGC
Cuts at: 1015 1660 1015
Size: 645 4307
BglII A'GATC_T
Cuts at: 548 1373 2467 4504 548
Size: 825 1094 2037 996
Fragments arranged by size:
2037 1094 996 825
BlpI GC'TnA_GC
Cuts at: 1315 2064 1315
Size: 749 4203
Bme1580I G_kGCm'C
Cuts at: 243 728 1121 2577 2670 3726 243
Size: 485 393 1456 93 1056 1469
Fragments arranged by size:
1469 1456 1056 485 393 93
BmrI ACTGGGnnnn_n'
Cuts at: 1738 2164 2245 2580 4724 1738
Size: 426 81 335 2144 1966
Fragments arranged by size:
2144 1966 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1119 3319 1119
Size: 2200 2752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 517 658 1114 486
Size: 31 141 456 4324
Fragments arranged by size:
4324 456 141 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 2128 3007 3499 3797 151
Size: 1977 879 492 298 1306
Fragments arranged by size:
1977 1306 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4656 4656
Size: 4952
BsaAI yAC'GTr
Cuts at: 2305 2305
Size: 4952
BsaBI GATnn'nnATC
Cuts at: 6 958 2466 4503 6
Size: 952 1508 2037 455
Fragments arranged by size:
2037 1508 952 455
BsaHI Gr'CG_yC
Cuts at: 272 418 2383 2631 3333 4829 272
Size: 146 1965 248 702 1496 395
Fragments arranged by size:
1965 1496 702 395 248 146
BsaWI w'CCGG_w
Cuts at: 2141 2662 3340 3614 3761 4172 2141
Size: 521 678 274 147 411 2921
Fragments arranged by size:
2921 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 571 343
Size: 228 4724
BseYI C'CCAG_C
Cuts at: 2377 3135 3712 4208 2377
Size: 758 577 496 3121
Fragments arranged by size:
3121 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2494 2540 3748 104
Size: 2390 46 1208 1308
Fragments arranged by size:
2390 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 243 321 483 1170 1278 1350 1966 2744 2934
Size: 78 162 687 108 72 616 778 190
Cuts at: 2934 3726 243
Size: 792 1469
Fragments arranged by size:
1469 792 778 687 616 190 162 108
78 72
BsmI GAATG_Cn'
Cuts at: 1820 1822 1820
Size: 2 4950
BsmBI CGTCTCn'nnnn_
Cuts at: 293 293
Size: 4952
Bsp1286I G_dGCh'C
Cuts at: 117 243 315 321 483 728 1121 1170 1278
Size: 126 72 6 162 245 393 49 108
Cuts at: 1278 1350 1966 2577 2670 2744 2934 2996 3726
Size: 72 616 611 93 74 190 62 730
Cuts at: 3726 117
Size: 1343
Fragments arranged by size:
1343 730 616 611 393 245 190 162
126 108 93 74 72 72 62 49
6
BspEI T'CCGG_A
Cuts at: 4172 4172
Size: 4952
BspHI T'CATG_A
Cuts at: 4128 4128
Size: 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 1495 2002 2216 2518 2899 377
Size: 1118 507 214 302 381 2430
Fragments arranged by size:
2430 1118 507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3244 3298 4782 4947 50
Size: 3194 54 1484 165 55
Fragments arranged by size:
3194 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1941 2864 1941
Size: 923 4029
BsrFI r'CCGG_y
Cuts at: 372 833 928 2950 3131 3340 372
Size: 461 95 2022 181 209 1984
Fragments arranged by size:
2022 1984 461 209 181 95
BsrGI T'GTAC_A
Cuts at: 1582 1582
Size: 4952
BssHII G'CGCG_C
Cuts at: 122 122
Size: 4952
BssSI C'ACGA_G
Cuts at: 1069 3223 3581 1069
Size: 2154 358 2440
Fragments arranged by size:
2440 2154 358
BstAPI GCAn_nnn'nTGC
Cuts at: 965 2329 965
Size: 1364 3588
BstBI TT'CG_AA
Cuts at: 3313 3313
Size: 4952
BstEII G'GTnAC_C
Cuts at: 1839 1839
Size: 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 717 802 717
Size: 85 4867
BstYI r'GATC_y
Cuts at: 1 548 775 1373 1977 2444 2467 2802 3048
Size: 547 227 598 604 467 23 335 246
Cuts at: 3048 4060 4146 4158 4504 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 604 598 547 467 449 346 335
246 227 86 23 12
BtgI C'CryG_G
Cuts at: 261 524 911 1871 261
Size: 263 387 960 3342
Fragments arranged by size:
3342 960 387 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2902 3083 3246 2902
Size: 181 163 4608
Fragments arranged by size:
4608 181 163
BtsI GCAGTG_nn'
Cuts at: 2195 2195
Size: 4952
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 876 2658 3516 876
Size: 1782 858 2312
Fragments arranged by size:
2312 1782 858
EaeI y'GGCC_r
Cuts at: 652 707 1007 1341 2537 2711 3102 3129 4474
Size: 55 300 334 1196 174 391 27 1345
Cuts at: 4474 652
Size: 1130
Fragments arranged by size:
1345 1196 1130 391 334 300 174 55
27
EagI C'GGCC_G
Cuts at: 2537 2537
Size: 4952
EarI CTCTTCn'nnn_
Cuts at: 186 544 557 2975 3185 186
Size: 358 13 2418 210 1953
Fragments arranged by size:
2418 1953 358 210 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 427 1297 3160 3470 3616 4762 427
Size: 870 1863 310 146 1146 617
Fragments arranged by size:
1863 1146 870 617 310 146
EcoICRI GAG'CTC
Cuts at: 1276 1348 1964 1276
Size: 72 616 4264
Fragments arranged by size:
4264 616 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 517 529 658 868 1114 1758 2777 3209
Size: 31 12 129 210 246 644 1019 432
Cuts at: 3209 3956 4224 486
Size: 747 268 1214
Fragments arranged by size:
1214 1019 747 644 432 268 246 210
129 31 12
EcoO109I rG'GnC_Cy
Cuts at: 355 366 439 571 808 2091 355
Size: 11 73 132 237 1283 3216
Fragments arranged by size:
3216 1283 237 132 73 11
EcoRI G'AATT_C
Cuts at: 1956 1956
Size: 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 873 2971 873
Size: 2098 2854
FspI TGC'GCA
Cuts at: 2733 2733
Size: 4952
HaeII r_GCGC'y
Cuts at: 261 584 763 2386 2634 3656 4635 261
Size: 323 179 1623 248 1022 979 578
Fragments arranged by size:
1623 1022 979 578 323 248 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2794 2824 2826 2856 2794
Size: 30 2 30 4890
Fragments arranged by size:
4890 30 30 2
HincII GTy'rAC
Cuts at: 1991 1991
Size: 4952
KasI G'GCGC_C
Cuts at: 2382 2630 2382
Size: 248 4704
KpnI G_GTAC'C
Cuts at: 1972 1972
Size: 4952
MfeI C'AATT_G
Cuts at: 740 740
Size: 4952
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 469 1019 1914 3623 3807 469
Size: 550 895 1709 184 1614
Fragments arranged by size:
1709 1614 895 550 184
MscI TGG'CCA
Cuts at: 654 1009 2713 654
Size: 355 1704 2893
Fragments arranged by size:
2893 1704 355
MslI CAynn'nnrTG
Cuts at: 2941 3068 2941
Size: 127 4825
MspA1I CmG'CkG
Cuts at: 263 867 1056 1314 2063 2377 2737 3750 3995
Size: 604 189 258 749 314 360 1013 245
Cuts at: 3995 263
Size: 1220
Fragments arranged by size:
1220 1013 749 604 360 314 258 245
189
NaeI GCC'GGC
Cuts at: 374 3133 374
Size: 2759 2193
NarI GG'CG_CC
Cuts at: 2383 2631 2383
Size: 248 4704
NgoMIV G'CCGG_C
Cuts at: 372 3131 372
Size: 2759 2193
NsiI A_TGCA'T
Cuts at: 1822 1822
Size: 4952
NspI r_CATG'y
Cuts at: 141 984 2005 2505 3412 141
Size: 843 1021 500 907 1681
Fragments arranged by size:
1681 1021 907 843 500
PasI CC'CwG_GG
Cuts at: 323 323
Size: 4952
PciI A'CATG_T
Cuts at: 3408 3408
Size: 4952
PflMI CCAn_nnn'nTGG
Cuts at: 1085 1085
Size: 4952
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1868 1900 4091 4123 1868
Size: 32 2191 32 2697
Fragments arranged by size:
2697 2191 32 32
PpuMI rG'GwC_Cy
Cuts at: 355 366 439 571 808 355
Size: 11 73 132 237 4499
Fragments arranged by size:
4499 237 132 73 11
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 844 876 844
Size: 32 4920
PstI C_TGCA'G
Cuts at: 402 1999 2684 402
Size: 1597 685 2670
Fragments arranged by size:
2670 1597 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 1314 2377 2737 1314
Size: 1063 360 3529
Fragments arranged by size:
3529 1063 360
RsrII CG'GwC_CG
Cuts at: 3147 3147
Size: 4952
SacI G_AGCT'C
Cuts at: 1278 1350 1966 1278
Size: 72 616 4264
Fragments arranged by size:
4264 616 72
SacII CC_GC'GG
Cuts at: 264 264
Size: 4952
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SanDI GG'GwC_CC
Cuts at: 355 571 808 355
Size: 216 237 4499
Fragments arranged by size:
4499 237 216
SapI GCTCTTCn'nnn_
Cuts at: 544 2975 3185 544
Size: 2431 210 2311
Fragments arranged by size:
2431 2311 210
SbfI CC_TGCA'GG
Cuts at: 1999 1999
Size: 4952
ScaI AGT'ACT
Cuts at: 4469 4469
Size: 4952
SfcI C'TryA_G
Cuts at: 33 245 398 1664 1995 2680 3673 3864 33
Size: 212 153 1266 331 685 993 191 1121
Fragments arranged by size:
1266 1121 993 685 331 212 191 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 1015 1015
Size: 4952
SfoI GGC'GCC
Cuts at: 2384 2632 2384
Size: 248 4704
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 119 383 437 752 1974 119
Size: 264 54 315 1222 3097
Fragments arranged by size:
3097 1222 315 264 54
SmlI C'TyrA_G
Cuts at: 166 2107 3022 3514 3776 166
Size: 1941 915 492 262 1342
Fragments arranged by size:
1941 1342 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 2005 2505 2005
Size: 500 4452
StyI C'CwwG_G
Cuts at: 655 779 2086 2440 655
Size: 124 1307 354 3167
Fragments arranged by size:
3167 1307 354 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 336 772 1257 2535 2668 3127 3336 336
Size: 436 485 1278 133 459 209 1952
Fragments arranged by size:
1952 1278 485 459 436 209 133
TatI w'GTAC_w
Cuts at: 1211 1582 1865 4467 1211
Size: 371 283 2602 1696
Fragments arranged by size:
2602 1696 371 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4469 4501 4469
Size: 32 4920
Tth111I GACn'n_nGTC
Cuts at: 2749 2749
Size: 4952
XbaI T'CTAG_A
Cuts at: 1983 1983
Size: 4952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 461 880 1002 2447 461
Size: 419 122 1445 2966
Fragments arranged by size:
2966 1445 419 122
XmaI C'CCGG_G
Cuts at: 117 381 435 750 1972 117
Size: 264 54 315 1222 3097
Fragments arranged by size:
3097 1222 315 264 54
XmnI GAAnn'nnTTC
Cuts at: 856 1301 1820 856
Size: 445 519 3988
Fragments arranged by size:
3988 519 445
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI PacI PfoI PmlI PshAI PsiI PspOMI PspXI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI