Restriction map for
pF1KB7062
(Circular) (Six-base) MAPSORT of: pF1KB7062.seq Check: 103 from: 1 to: 5105
pF1KB7062 5105 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 126 126
Size: 5105
AccI GT'mk_AC
Cuts at: 1269 1490 1874 2143 1269
Size: 221 384 269 4231
Fragments arranged by size:
4231 384 269 221
Acc65I G'GTAC_C
Cuts at: 2121 2121
Size: 5105
AclI AA'CG_TT
Cuts at: 4945 4945
Size: 5105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 721 1099 2096 2930 3362 4109 4377 365
Size: 356 378 997 834 432 747 268 1093
Fragments arranged by size:
1093 997 834 747 432 378 356 268
AflII C'TTAA_G
Cuts at: 876 1161 876
Size: 285 4820
AflIII A'CryG_T
Cuts at: 347 437 749 1172 2457 3561 4538 347
Size: 90 312 423 1285 1104 977 914
Fragments arranged by size:
1285 1104 977 914 423 312 90
AgeI A'CCGG_T
Cuts at: 3493 3493
Size: 5105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1364 1364
Size: 5105
AlwNI CAG_nnn'CTG
Cuts at: 1225 2074 2410 3977 1225
Size: 849 336 1567 2353
Fragments arranged by size:
2353 1567 849 336
ApaI G_GGCC'C
Cuts at: 125 125
Size: 5105
ApaLI G'TGCA_C
Cuts at: 3875 3875
Size: 5105
ApoI r'AATT_y
Cuts at: 758 1522 1589 2080 2109 4458 4714 758
Size: 764 67 491 29 2349 256 1149
Fragments arranged by size:
2349 1149 764 491 256 67 29
AseI AT'TA_AT
Cuts at: 20 568 1231 1694 20
Size: 548 663 463 3431
Fragments arranged by size:
3431 663 548 463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
AvaI C'yCGr_G
Cuts at: 2125 2125
Size: 5105
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2084 2117 2084
Size: 33 5072
BamHI G'GATC_C
Cuts at: 2130 2130
Size: 5105
BanI G'GyrC_C
Cuts at: 189 427 2121 2535 2783 2818 189
Size: 238 1694 414 248 35 2476
Fragments arranged by size:
2476 1694 414 248 238 35
BanII G_rGCy'C
Cuts at: 125 2119 3149 125
Size: 1994 1030 2081
Fragments arranged by size:
2081 1994 1030
BbeI G_GCGC'C
Cuts at: 2539 2787 2539
Size: 248 4857
BbsI GAAGACnn'nnnn_
Cuts at: 1663 1663
Size: 5105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 770 736
Size: 34 5071
BciVI GTATCCnnnnn_n'
Cuts at: 520 3003 3764 5105 520
Size: 2483 761 1341 520
Fragments arranged by size:
2483 1341 761 520
BclI T'GATC_A
Cuts at: 2625 2625
Size: 5105
BfrBI ATG'CAT
Cuts at: 1618 1618
Size: 5105
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5105
BglII A'GATC_T
Cuts at: 269 2620 4657 269
Size: 2351 2037 717
Fragments arranged by size:
2351 2037 717
BlpI GC'TnA_GC
Cuts at: 357 2217 357
Size: 1860 3245
Bme1580I G_kGCm'C
Cuts at: 125 194 2730 2823 3879 125
Size: 69 2536 93 1056 1351
Fragments arranged by size:
2536 1351 1056 93 69
BmgBI CAC'GTC
Cuts at: 440 440
Size: 5105
BmrI ACTGGGnnnn_n'
Cuts at: 991 1130 2317 2398 2733 4877 991
Size: 139 1187 81 335 2144 1219
Fragments arranged by size:
2144 1219 1187 335 139 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 3472
Size: 5105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 526 1931 250
Size: 276 1405 3424
Fragments arranged by size:
3424 1405 276
Bpu10I CC'TnA_GC
Cuts at: 851 851
Size: 5105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 3160 3652 3950 2281
Size: 879 492 298 3436
Fragments arranged by size:
3436 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4809 4809
Size: 5105
BsaAI yAC'GTr
Cuts at: 350 2458 350
Size: 2108 2997
BsaBI GATnn'nnATC
Cuts at: 6 2619 4656 6
Size: 2613 2037 455
Fragments arranged by size:
2613 2037 455
BsaHI Gr'CG_yC
Cuts at: 212 1071 2536 2784 3486 4982 212
Size: 859 1465 248 702 1496 335
Fragments arranged by size:
1496 1465 859 702 335 248
BsaWI w'CCGG_w
Cuts at: 2294 2815 3493 3767 3914 4325 2294
Size: 521 678 274 147 411 3074
Fragments arranged by size:
3074 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2530 3288 3865 4361 2530
Size: 758 577 496 3274
Fragments arranged by size:
3274 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 2647 2693 3901 104
Size: 12 2531 46 1208 1308
Fragments arranged by size:
2531 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 266 2119 2897 3087 3879 266
Size: 1853 778 190 792 1492
Fragments arranged by size:
1853 1492 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 125 194 266 2119 2730 2823 2897 3087 3149
Size: 69 72 1853 611 93 74 190 62
Cuts at: 3149 3879 125
Size: 730 1351
Fragments arranged by size:
1853 1351 730 611 190 93 74 72
69 62
BspEI T'CCGG_A
Cuts at: 4325 4325
Size: 5105
BspHI T'CATG_A
Cuts at: 1439 1761 4281 1439
Size: 322 2520 2263
Fragments arranged by size:
2520 2263 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 147 2155 2369 2671 3052 126
Size: 21 2008 214 302 381 2179
Fragments arranged by size:
2179 2008 381 302 214 21
BsrBI CCG'CTC
Cuts at: 50 3397 3451 4935 5100 50
Size: 3347 54 1484 165 55
Fragments arranged by size:
3347 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3017 3017
Size: 5105
BsrFI r'CCGG_y
Cuts at: 248 3103 3284 3493 248
Size: 2855 181 209 1860
Fragments arranged by size:
2855 1860 209 181
BsrGI T'GTAC_A
Cuts at: 869 1835 869
Size: 966 4139
BssSI C'ACGA_G
Cuts at: 3376 3734 3376
Size: 358 4747
BstAPI GCAn_nnn'nTGC
Cuts at: 138 2482 138
Size: 2344 2761
BstBI TT'CG_AA
Cuts at: 3466 3466
Size: 5105
BstYI r'GATC_y
Cuts at: 1 269 579 2130 2597 2620 2955 3201 4213
Size: 268 310 1551 467 23 335 246 1012
Cuts at: 4213 4299 4311 4657 1
Size: 86 12 346 449
Fragments arranged by size:
1551 1012 467 449 346 335 310 268
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1875 1875
Size: 5105
BtgI C'CryG_G
Cuts at: 107 116 1209 107
Size: 9 1093 4003
Fragments arranged by size:
4003 1093 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3055 3236 3399 3055
Size: 181 163 4761
Fragments arranged by size:
4761 181 163
BtsI GCAGTG_nn'
Cuts at: 698 2348 698
Size: 1650 3455
DraI TTT'AAA
Cuts at: 2104 2104
Size: 5105
DrdI GACnn_nn'nnGTC
Cuts at: 1486 2811 3669 1486
Size: 1325 858 2922
Fragments arranged by size:
2922 1325 858
EaeI y'GGCC_r
Cuts at: 113 2690 2864 3255 3282 4627 113
Size: 2577 174 391 27 1345 591
Fragments arranged by size:
2577 1345 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2690 113
Size: 2577 2528
EarI CTCTTCn'nnn_
Cuts at: 790 1118 1139 3128 3338 790
Size: 328 21 1989 210 2557
Fragments arranged by size:
2557 1989 328 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3313 3623 3769 4915 3313
Size: 310 146 1146 3503
Fragments arranged by size:
3503 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2117 2117
Size: 5105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 365 526 721 1099 1931 2096 2930 3362
Size: 115 161 195 378 832 165 834 432
Cuts at: 3362 4109 4377 250
Size: 747 268 978
Fragments arranged by size:
978 834 832 747 432 378 268 195
165 161 115
EcoNI CCTnn'n_nnAGG
Cuts at: 470 596 470
Size: 126 4979
EcoO109I rG'GnC_Cy
Cuts at: 121 122 475 930 2244 121
Size: 1 353 455 1314 2982
Fragments arranged by size:
2982 1314 455 353 1
EcoRI G'AATT_C
Cuts at: 2109 2109
Size: 5105
EcoRV GAT'ATC
Cuts at: 1890 1890
Size: 5105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1168 3124 1168
Size: 1956 3149
FspI TGC'GCA
Cuts at: 2886 2886
Size: 5105
HaeII r_GCGC'y
Cuts at: 172 184 2539 2787 3809 4788 172
Size: 12 2355 248 1022 979 489
Fragments arranged by size:
2355 1022 979 489 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 2947 2977 2979 3009 135
Size: 32 2780 30 2 30 2231
Fragments arranged by size:
2780 2231 32 30 30 2
HincII GTy'rAC
Cuts at: 2144 2144
Size: 5105
HindIII A'AGCT_T
Cuts at: 1474 1474
Size: 5105
KasI G'GCGC_C
Cuts at: 2535 2783 2535
Size: 248 4857
KpnI G_GTAC'C
Cuts at: 2125 2125
Size: 5105
MluI A'CGCG_T
Cuts at: 4538 4538
Size: 5105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3776 3960 3776
Size: 184 4921
MscI TGG'CCA
Cuts at: 2866 2866
Size: 5105
MslI CAynn'nnrTG
Cuts at: 1346 1567 3094 3221 1346
Size: 221 1527 127 3230
Fragments arranged by size:
3230 1527 221 127
MspA1I CmG'CkG
Cuts at: 2074 2216 2530 2890 3903 4148 2074
Size: 142 314 360 1013 245 3031
Fragments arranged by size:
3031 1013 360 314 245 142
NaeI GCC'GGC
Cuts at: 3286 3286
Size: 5105
NarI GG'CG_CC
Cuts at: 2536 2784 2536
Size: 248 4857
NcoI C'CATG_G
Cuts at: 107 1209 107
Size: 1102 4003
NgoMIV G'CCGG_C
Cuts at: 3284 3284
Size: 5105
NsiI A_TGCA'T
Cuts at: 1620 1620
Size: 5105
NspI r_CATG'y
Cuts at: 753 1176 1618 2158 2658 3565 753
Size: 423 442 540 500 907 2293
Fragments arranged by size:
2293 907 540 500 442 423
PciI A'CATG_T
Cuts at: 749 1172 3561 749
Size: 423 2389 2293
Fragments arranged by size:
2389 2293 423
PflMI CCAn_nnn'nTGG
Cuts at: 1020 1020
Size: 5105
PmeI GTTT'AAAC
Cuts at: 2104 2104
Size: 5105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 5073
PpuMI rG'GwC_Cy
Cuts at: 475 930 475
Size: 455 4650
PshAI GACnn'nnGTC
Cuts at: 1070 1070
Size: 5105
PsiI TTA'TAA
Cuts at: 949 949
Size: 5105
PspOMI G'GGCC_C
Cuts at: 121 121
Size: 5105
PstI C_TGCA'G
Cuts at: 1194 2152 2837 1194
Size: 958 685 3462
Fragments arranged by size:
3462 958 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5105
PvuII CAG'CTG
Cuts at: 2074 2530 2890 2074
Size: 456 360 4289
Fragments arranged by size:
4289 456 360
RsrII CG'GwC_CG
Cuts at: 3300 3300
Size: 5105
SacI G_AGCT'C
Cuts at: 2119 2119
Size: 5105
SalI G'TCGA_C
Cuts at: 2142 2142
Size: 5105
SapI GCTCTTCn'nnn_
Cuts at: 1118 3128 3338 1118
Size: 2010 210 2885
Fragments arranged by size:
2885 2010 210
SbfI CC_TGCA'GG
Cuts at: 2152 2152
Size: 5105
ScaI AGT'ACT
Cuts at: 4622 4622
Size: 5105
SfcI C'TryA_G
Cuts at: 33 505 857 1190 2148 2833 3826 4017 33
Size: 472 352 333 958 685 993 191 1121
Fragments arranged by size:
1121 993 958 685 472 352 333 191
SfoI GGC'GCC
Cuts at: 2537 2785 2537
Size: 248 4857
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
SmaI CCC'GGG
Cuts at: 2127 2127
Size: 5105
SmlI C'TyrA_G
Cuts at: 876 1161 2260 3175 3667 3929 876
Size: 285 1099 915 492 262 2052
Fragments arranged by size:
2052 1099 915 492 285 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5105
SphI G_CATG'C
Cuts at: 2158 2658 2158
Size: 500 4605
SspI AAT'ATT
Cuts at: 1034 1034
Size: 5105
StyI C'CwwG_G
Cuts at: 107 1209 2239 2593 107
Size: 1102 1030 354 2619
Fragments arranged by size:
2619 1102 1030 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 947 1950 2688 2821 3280 3489 947
Size: 1003 738 133 459 209 2563
Fragments arranged by size:
2563 1003 738 459 209 133
TatI w'GTAC_w
Cuts at: 869 978 1835 2008 4620 869
Size: 109 857 173 2612 1354
Fragments arranged by size:
2612 1354 857 173 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4622 4654 4622
Size: 32 5073
Tth111I GACn'n_nGTC
Cuts at: 2902 2902
Size: 5105
XbaI T'CTAG_A
Cuts at: 2136 2136
Size: 5105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2600 114
Size: 2486 2619
XmaI C'CCGG_G
Cuts at: 2125 2125
Size: 5105
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BbvCI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI