Restriction map for
pF1KB7064
(Circular) (Six-base) MAPSORT of: pF1KB7064.seq Check: 985 from: 1 to: 5135
pF1KB7064 5135 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 2173 2173
Size: 5135
Acc65I G'GTAC_C
Cuts at: 2151 2151
Size: 5135
AclI AA'CG_TT
Cuts at: 4975 4975
Size: 5135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 377 443 635 848 1144 1527 2960 3392
Size: 12 66 192 213 296 383 1433 432
Cuts at: 3392 4139 4407 365
Size: 747 268 1093
Fragments arranged by size:
1433 1093 747 432 383 296 268 213
192 66 12
AflIII A'CryG_T
Cuts at: 2487 3591 4568 2487
Size: 1104 977 3054
Fragments arranged by size:
3054 1104 977
AgeI A'CCGG_T
Cuts at: 3523 3523
Size: 5135
AhdI GACnn_n'nnGTC
Cuts at: 879 879
Size: 5135
AleI CACnn'nnGTG
Cuts at: 766 766
Size: 5135
AlwNI CAG_nnn'CTG
Cuts at: 884 1550 1952 2101 2440 4007 884
Size: 666 402 149 339 1567 2012
Fragments arranged by size:
2012 1567 666 402 339 149
ApaI G_GGCC'C
Cuts at: 1539 1849 1539
Size: 310 4825
ApaLI G'TGCA_C
Cuts at: 3905 3905
Size: 5135
ApoI r'AATT_y
Cuts at: 2139 4488 4744 2139
Size: 2349 256 2530
Fragments arranged by size:
2530 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5135
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
AvaI C'yCGr_G
Cuts at: 1865 1940 2155 1865
Size: 75 215 4845
Fragments arranged by size:
4845 215 75
AvrII C'CTAG_G
Cuts at: 1681 1681
Size: 5135
BamHI G'GATC_C
Cuts at: 748 2160 748
Size: 1412 3723
BanI G'GyrC_C
Cuts at: 1016 2151 2565 2813 2848 1016
Size: 1135 414 248 35 3303
Fragments arranged by size:
3303 1135 414 248 35
BanII G_rGCy'C
Cuts at: 239 1539 1619 1677 1849 1921 2149 3179 239
Size: 1300 80 58 172 72 228 1030 2195
Fragments arranged by size:
2195 1300 1030 228 172 80 72 58
BbeI G_GCGC'C
Cuts at: 2569 2817 2569
Size: 248 4887
BbsI GAAGACnn'nnnn_
Cuts at: 365 847 1251 365
Size: 482 404 4249
Fragments arranged by size:
4249 482 404
BbvCI CC'TCA_GC
Cuts at: 193 978 1226 1595 193
Size: 785 248 369 3733
Fragments arranged by size:
3733 785 369 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1846 1863 1880 1897 1846
Size: 17 17 17 5084
Fragments arranged by size:
5084 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 3033 3794 5135 3033
Size: 761 1341 3033
Fragments arranged by size:
3033 1341 761
BclI T'GATC_A
Cuts at: 2655 2655
Size: 5135
BglI GCCn_nnn'nGGC
Cuts at: 218 218
Size: 5135
BglII A'GATC_T
Cuts at: 2650 4687 2650
Size: 2037 3098
BlpI GC'TnA_GC
Cuts at: 1613 1915 2247 1613
Size: 302 332 4501
Fragments arranged by size:
4501 332 302
Bme1580I G_kGCm'C
Cuts at: 1539 1849 1959 2760 2853 3909 1539
Size: 310 110 801 93 1056 2765
Fragments arranged by size:
2765 1056 801 310 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 172 403 953 1019 1611 2029 2347 2428 2763
Size: 231 550 66 592 418 318 81 335
Cuts at: 2763 4907 172
Size: 2144 400
Fragments arranged by size:
2144 592 550 418 400 335 318 231
81 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 3502 1251
Size: 2251 2884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 307 395 728 1670 225
Size: 82 88 333 942 3690
Fragments arranged by size:
3690 942 333 88 82
Bpu10I CC'TnA_GC
Cuts at: 193 978 1226 1595 193
Size: 785 248 369 3733
Fragments arranged by size:
3733 785 369 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1696 2073 2311 3190 3682 3980 988
Size: 708 377 238 879 492 298 2143
Fragments arranged by size:
2143 879 708 492 377 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 4839 4839
Size: 5135
BsaAI yAC'GTr
Cuts at: 2488 2488
Size: 5135
BsaBI GATnn'nnATC
Cuts at: 6 2649 4686 6
Size: 2643 2037 455
Fragments arranged by size:
2643 2037 455
BsaHI Gr'CG_yC
Cuts at: 339 907 2566 2814 3516 5012 339
Size: 568 1659 248 702 1496 462
Fragments arranged by size:
1659 1496 702 568 462 248
BsaWI w'CCGG_w
Cuts at: 1740 2324 2845 3523 3797 3944 4355 1740
Size: 584 521 678 274 147 411 2520
Fragments arranged by size:
2520 678 584 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 296 364 601 634 694 697 928 1051
Size: 115 68 237 33 60 3 231 123
Cuts at: 1051 1315 1318 1444 1447 1667 1924 2007 181
Size: 264 3 126 3 220 257 83 3309
Fragments arranged by size:
3309 264 257 237 231 220 126 123
115 83 68 60 33 3 3 3
BseYI C'CCAG_C
Cuts at: 538 757 984 998 1550 2560 3318 3895 4391
Size: 219 227 14 552 1010 758 577 496
Cuts at: 4391 538
Size: 1282
Fragments arranged by size:
1282 1010 758 577 552 496 227 219
14
BsiEI CG_ry'CG
Cuts at: 104 2677 2723 3931 104
Size: 2573 46 1208 1308
Fragments arranged by size:
2573 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 528 2149 2927 3117 3909 528
Size: 1621 778 190 792 1754
Fragments arranged by size:
1754 1621 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 239 528 1539 1619 1677 1849 1921 1959 2149
Size: 289 1011 80 58 172 72 38 190
Cuts at: 2149 2760 2853 2927 3117 3179 3909 239
Size: 611 93 74 190 62 730 1465
Fragments arranged by size:
1465 1011 730 611 289 190 190 172
93 80 74 72 62 58 38
BspEI T'CCGG_A
Cuts at: 1740 4355 1740
Size: 2615 2520
BspHI T'CATG_A
Cuts at: 1734 4311 1734
Size: 2577 2558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2185 2399 2701 3082 2185
Size: 214 302 381 4238
Fragments arranged by size:
4238 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2081 3427 3481 4965 5130 50
Size: 2031 1346 54 1484 165 55
Fragments arranged by size:
2031 1484 1346 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3047 3047
Size: 5135
BsrFI r'CCGG_y
Cuts at: 1824 3133 3314 3523 1824
Size: 1309 181 209 3436
Fragments arranged by size:
3436 1309 209 181
BssSI C'ACGA_G
Cuts at: 3406 3764 3406
Size: 358 4777
BstAPI GCAn_nnn'nTGC
Cuts at: 2101 2512 2101
Size: 411 4724
BstBI TT'CG_AA
Cuts at: 3496 3496
Size: 5135
BstYI r'GATC_y
Cuts at: 1 748 2160 2627 2650 2985 3231 4243 4329
Size: 747 1412 467 23 335 246 1012 86
Cuts at: 4329 4341 4687 1
Size: 12 346 449
Fragments arranged by size:
1412 1012 747 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 214 1963 214
Size: 1749 3386
BtgI C'CryG_G
Cuts at: 107 142 674 800 107
Size: 35 532 126 4442
Fragments arranged by size:
4442 532 126 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3085 3266 3429 3085
Size: 181 163 4791
Fragments arranged by size:
4791 181 163
BtsI GCAGTG_nn'
Cuts at: 556 2378 556
Size: 1822 3313
DraI TTT'AAA
Cuts at: 2134 2134
Size: 5135
DraIII CAC_nnn'GTG
Cuts at: 1114 1114
Size: 5135
DrdI GACnn_nn'nnGTC
Cuts at: 849 2841 3699 849
Size: 1992 858 2285
Fragments arranged by size:
2285 1992 858
EaeI y'GGCC_r
Cuts at: 516 1141 1411 1634 2066 2720 2894 3285 3312
Size: 625 270 223 432 654 174 391 27
Cuts at: 3312 4657 516
Size: 1345 994
Fragments arranged by size:
1345 994 654 625 432 391 270 223
174 27
EagI C'GGCC_G
Cuts at: 2720 2720
Size: 5135
EarI CTCTTCn'nnn_
Cuts at: 341 596 611 782 1028 1157 1208 1242 1577
Size: 255 15 171 246 129 51 34 335
Cuts at: 1577 3158 3368 341
Size: 1581 210 2108
Fragments arranged by size:
2108 1581 335 255 246 210 171 129
51 34 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3343 3653 3799 4945 3343
Size: 310 146 1146 3533
Fragments arranged by size:
3533 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2147 2147
Size: 5135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 307 365 377 395 443 635 728 848
Size: 82 58 12 18 48 192 93 120
Cuts at: 848 1144 1527 1670 2960 3392 4139 4407 225
Size: 296 383 143 1290 432 747 268 953
Fragments arranged by size:
1290 953 747 432 383 296 268 192
143 120 93 82 58 48 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1250 1250
Size: 5135
EcoO109I rG'GnC_Cy
Cuts at: 219 226 370 881 995 1535 1845 1846 2274
Size: 7 144 511 114 540 310 1 428
Cuts at: 2274 219
Size: 3080
Fragments arranged by size:
3080 540 511 428 310 144 114 7
1
EcoRI G'AATT_C
Cuts at: 2139 2139
Size: 5135
EcoRV GAT'ATC
Cuts at: 1558 1558
Size: 5135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1794 3154 1794
Size: 1360 3775
FseI GG_CCGG'CC
Cuts at: 1828 1828
Size: 5135
FspI TGC'GCA
Cuts at: 2916 2916
Size: 5135
HaeII r_GCGC'y
Cuts at: 1719 2569 2817 3839 4818 1719
Size: 850 248 1022 979 2036
Fragments arranged by size:
2036 1022 979 850 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 1180 1186 1212 1218 1315 1347 1622
Size: 32 283 6 26 6 97 32 275
Cuts at: 1622 1654 2977 3007 3009 3039 865
Size: 32 1323 30 2 30 2961
Fragments arranged by size:
2961 1323 283 275 97 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 630 2174 630
Size: 1544 3591
HpaI GTT'AAC
Cuts at: 630 630
Size: 5135
KasI G'GCGC_C
Cuts at: 2565 2813 2565
Size: 248 4887
KpnI G_GTAC'C
Cuts at: 2155 2155
Size: 5135
MluI A'CGCG_T
Cuts at: 4568 4568
Size: 5135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 896 1719 3806 3990 896
Size: 823 2087 184 2041
Fragments arranged by size:
2087 2041 823 184
MscI TGG'CCA
Cuts at: 518 1143 1636 2068 2896 518
Size: 625 493 432 828 2757
Fragments arranged by size:
2757 828 625 493 432
MslI CAynn'nnrTG
Cuts at: 766 3124 3251 766
Size: 2358 127 2650
Fragments arranged by size:
2650 2358 127
MspA1I CmG'CkG
Cuts at: 1066 2098 2246 2560 2920 3933 4178 1066
Size: 1032 148 314 360 1013 245 2023
Fragments arranged by size:
2023 1032 1013 360 314 245 148
NaeI GCC'GGC
Cuts at: 1826 3316 1826
Size: 1490 3645
NarI GG'CG_CC
Cuts at: 2566 2814 2566
Size: 248 4887
NcoI C'CATG_G
Cuts at: 107 107
Size: 5135
NgoMIV G'CCGG_C
Cuts at: 1824 3314 1824
Size: 1490 3645
NspI r_CATG'y
Cuts at: 459 2188 2688 3595 459
Size: 1729 500 907 1999
Fragments arranged by size:
1999 1729 907 500
PasI CC'CwG_GG
Cuts at: 580 778 964 1670 1850 580
Size: 198 186 706 180 3865
Fragments arranged by size:
3865 706 198 186 180
PciI A'CATG_T
Cuts at: 3591 3591
Size: 5135
PflMI CCAn_nnn'nTGG
Cuts at: 764 884 1806 1952 2064 764
Size: 120 922 146 112 3835
Fragments arranged by size:
3835 922 146 120 112
PfoI T'CCnGG_A
Cuts at: 744 744
Size: 5135
PmeI GTTT'AAAC
Cuts at: 2134 2134
Size: 5135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 5103
PpuMI rG'GwC_Cy
Cuts at: 226 881 226
Size: 655 4480
PspOMI G'GGCC_C
Cuts at: 1535 1845 1535
Size: 310 4825
PstI C_TGCA'G
Cuts at: 2097 2182 2867 2097
Size: 85 685 4365
Fragments arranged by size:
4365 685 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5135
PvuII CAG'CTG
Cuts at: 1066 2560 2920 1066
Size: 1494 360 3281
Fragments arranged by size:
3281 1494 360
RsrII CG'GwC_CG
Cuts at: 3330 3330
Size: 5135
SacI G_AGCT'C
Cuts at: 2149 2149
Size: 5135
SalI G'TCGA_C
Cuts at: 2172 2172
Size: 5135
SapI GCTCTTCn'nnn_
Cuts at: 3158 3368 3158
Size: 210 4925
SbfI CC_TGCA'GG
Cuts at: 2182 2182
Size: 5135
ScaI AGT'ACT
Cuts at: 4652 4652
Size: 5135
SfcI C'TryA_G
Cuts at: 33 1250 2093 2178 2863 3856 4047 33
Size: 1217 843 85 685 993 191 1121
Fragments arranged by size:
1217 1121 993 843 685 191 85
SfoI GGC'GCC
Cuts at: 2567 2815 2567
Size: 248 4887
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
SmaI CCC'GGG
Cuts at: 1942 2157 1942
Size: 215 4920
SmlI C'TyrA_G
Cuts at: 1003 1711 2052 2290 3205 3697 3959 1003
Size: 708 341 238 915 492 262 2179
Fragments arranged by size:
2179 915 708 492 341 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5135
SphI G_CATG'C
Cuts at: 2188 2688 2188
Size: 500 4635
SrfI GCCC'GGGC
Cuts at: 1942 1942
Size: 5135
StuI AGG'CCT
Cuts at: 1129 1399 1129
Size: 270 4865
StyI C'CwwG_G
Cuts at: 107 328 1487 1681 2269 2623 107
Size: 221 1159 194 588 354 2619
Fragments arranged by size:
2619 1159 588 354 221 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 756 1122 1392 1912 2014 2718 2851 3310 3519
Size: 366 270 520 102 704 133 459 209
Cuts at: 3519 756
Size: 2372
Fragments arranged by size:
2372 704 520 459 366 270 209 133
102
TatI w'GTAC_w
Cuts at: 4650 4650
Size: 5135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5103
Tth111I GACn'n_nGTC
Cuts at: 2932 2932
Size: 5135
XbaI T'CTAG_A
Cuts at: 231 2166 231
Size: 1935 3200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1667 2630 1667
Size: 963 4172
XmaI C'CCGG_G
Cuts at: 1940 2155 1940
Size: 215 4920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI BaeI
BfrBI BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI