Restriction map for
pF1KB7085
(Circular) (Six-base) MAPSORT of: pF1KB7085.seq Check: 2668 from: 1 to: 3500
pF1KB7085 3500 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 347 538 347
Size: 191 3309
Acc65I G'GTAC_C
Cuts at: 516 516
Size: 3500
AclI AA'CG_TT
Cuts at: 3340 3340
Size: 3500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1757 2504 2772 1325
Size: 432 747 268 2053
Fragments arranged by size:
2053 747 432 268
AflIII A'CryG_T
Cuts at: 185 852 1956 2933 185
Size: 667 1104 977 752
Fragments arranged by size:
1104 977 752 667
AgeI A'CCGG_T
Cuts at: 1888 1888
Size: 3500
AleI CACnn'nnGTG
Cuts at: 130 130
Size: 3500
AlwNI CAG_nnn'CTG
Cuts at: 805 2372 805
Size: 1567 1933
ApaLI G'TGCA_C
Cuts at: 2270 2270
Size: 3500
ApoI r'AATT_y
Cuts at: 504 2853 3109 504
Size: 2349 256 895
Fragments arranged by size:
2349 895 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3500
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
AvaI C'yCGr_G
Cuts at: 398 520 398
Size: 122 3378
BamHI G'GATC_C
Cuts at: 525 525
Size: 3500
BanI G'GyrC_C
Cuts at: 516 930 1178 1213 516
Size: 414 248 35 2803
Fragments arranged by size:
2803 414 248 35
BanII G_rGCy'C
Cuts at: 514 1544 514
Size: 1030 2470
BbeI G_GCGC'C
Cuts at: 934 1182 934
Size: 248 3252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 377 343
Size: 34 3466
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2159 3500 1398
Size: 761 1341 1398
Fragments arranged by size:
1398 1341 761
BclI T'GATC_A
Cuts at: 1020 1020
Size: 3500
BglII A'GATC_T
Cuts at: 1015 3052 1015
Size: 2037 1463
BlpI GC'TnA_GC
Cuts at: 295 612 295
Size: 317 3183
Bme1580I G_kGCm'C
Cuts at: 1125 1218 2274 1125
Size: 93 1056 2351
Fragments arranged by size:
2351 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 268 487 712 793 1128 3272 268
Size: 219 225 81 335 2144 496
Fragments arranged by size:
2144 496 335 225 219 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 340 427 253
Size: 87 87 3326
Fragments arranged by size:
3326 87 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1555 2047 2345 676
Size: 879 492 298 1831
Fragments arranged by size:
1831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3204 3204
Size: 3500
BsaAI yAC'GTr
Cuts at: 853 853
Size: 3500
BsaBI GATnn'nnATC
Cuts at: 6 1014 3051 6
Size: 1008 2037 455
Fragments arranged by size:
2037 1008 455
BsaHI Gr'CG_yC
Cuts at: 136 931 1179 1881 3377 136
Size: 795 248 702 1496 259
Fragments arranged by size:
1496 795 702 259 248
BsaWI w'CCGG_w
Cuts at: 689 1210 1888 2162 2309 2720 689
Size: 521 678 274 147 411 1469
Fragments arranged by size:
1469 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 292
Size: 30 3470
BseYI C'CCAG_C
Cuts at: 925 1683 2260 2756 925
Size: 758 577 496 1669
Fragments arranged by size:
1669 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1042 1088 2296 104
Size: 938 46 1208 1308
Fragments arranged by size:
1308 1208 938 46
BsiHKAI G_wGCw'C
Cuts at: 326 514 1292 1482 2274 326
Size: 188 778 190 792 1552
Fragments arranged by size:
1552 792 778 190 188
BsmBI CGTCTCn'nnnn_
Cuts at: 472 472
Size: 3500
Bsp1286I G_dGCh'C
Cuts at: 326 514 1125 1218 1292 1482 1544 2274 326
Size: 188 611 93 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 190 188 93 74 62
BspEI T'CCGG_A
Cuts at: 2720 2720
Size: 3500
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 194 550 764 1066 1447 194
Size: 356 214 302 381 2247
Fragments arranged by size:
2247 381 356 302 214
BsrBI CCG'CTC
Cuts at: 50 308 1792 1846 3330 3495 50
Size: 258 1484 54 1484 165 55
Fragments arranged by size:
1484 1484 258 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1412 1412
Size: 3500
BsrFI r'CCGG_y
Cuts at: 1498 1679 1888 1498
Size: 181 209 3110
Fragments arranged by size:
3110 209 181
BssSI C'ACGA_G
Cuts at: 1771 2129 1771
Size: 358 3142
BstAPI GCAn_nnn'nTGC
Cuts at: 877 877
Size: 3500
BstBI TT'CG_AA
Cuts at: 1861 1861
Size: 3500
BstXI CCAn_nnnn'nTGG
Cuts at: 318 318
Size: 3500
BstYI r'GATC_y
Cuts at: 1 525 992 1015 1350 1596 2608 2694 2706
Size: 524 467 23 335 246 1012 86 12
Cuts at: 2706 3052 1
Size: 346 449
Fragments arranged by size:
1012 524 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1450 1631 1794 1450
Size: 181 163 3156
Fragments arranged by size:
3156 181 163
BtsI GCAGTG_nn'
Cuts at: 743 743
Size: 3500
DraI TTT'AAA
Cuts at: 499 499
Size: 3500
DrdI GACnn_nn'nnGTC
Cuts at: 1206 2064 1206
Size: 858 2642
EaeI y'GGCC_r
Cuts at: 446 1085 1259 1650 1677 3022 446
Size: 639 174 391 27 1345 924
Fragments arranged by size:
1345 924 639 391 174 27
EagI C'GGCC_G
Cuts at: 1085 1085
Size: 3500
EarI CTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1708 2018 2164 3310 1708
Size: 310 146 1146 1898
Fragments arranged by size:
1898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 512 512
Size: 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 340 427 1325 1757 2504 2772 253
Size: 87 87 898 432 747 268 981
Fragments arranged by size:
981 898 747 432 268 87 87
EcoO109I rG'GnC_Cy
Cuts at: 206 237 402 639 206
Size: 31 165 237 3067
Fragments arranged by size:
3067 237 165 31
EcoRI G'AATT_C
Cuts at: 504 504
Size: 3500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1519 1519
Size: 3500
FspI TGC'GCA
Cuts at: 1281 1281
Size: 3500
HaeII r_GCGC'y
Cuts at: 934 1182 2204 3183 934
Size: 248 1022 979 1251
Fragments arranged by size:
1251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1342 1372 1374 1404 1342
Size: 30 2 30 3438
Fragments arranged by size:
3438 30 30 2
HincII GTy'rAC
Cuts at: 539 539
Size: 3500
KasI G'GCGC_C
Cuts at: 930 1178 930
Size: 248 3252
KpnI G_GTAC'C
Cuts at: 520 520
Size: 3500
MluI A'CGCG_T
Cuts at: 2933 2933
Size: 3500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2355 2171
Size: 184 3316
MscI TGG'CCA
Cuts at: 448 1261 448
Size: 813 2687
MslI CAynn'nnrTG
Cuts at: 130 1489 1616 130
Size: 1359 127 2014
Fragments arranged by size:
2014 1359 127
MspA1I CmG'CkG
Cuts at: 130 218 611 925 1285 2298 2543 130
Size: 88 393 314 360 1013 245 1087
Fragments arranged by size:
1087 1013 393 360 314 245 88
NaeI GCC'GGC
Cuts at: 1681 1681
Size: 3500
NarI GG'CG_CC
Cuts at: 931 1179 931
Size: 248 3252
NgoMIV G'CCGG_C
Cuts at: 1679 1679
Size: 3500
NspI r_CATG'y
Cuts at: 189 553 1053 1960 189
Size: 364 500 907 1729
Fragments arranged by size:
1729 907 500 364
PasI CC'CwG_GG
Cuts at: 282 282
Size: 3500
PciI A'CATG_T
Cuts at: 185 1956 185
Size: 1771 1729
PflMI CCAn_nnn'nTGG
Cuts at: 155 155
Size: 3500
PfoI T'CCnGG_A
Cuts at: 404 404
Size: 3500
PmeI GTTT'AAAC
Cuts at: 499 499
Size: 3500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 3468
PpuMI rG'GwC_Cy
Cuts at: 206 402 206
Size: 196 3304
PstI C_TGCA'G
Cuts at: 547 1232 547
Size: 685 2815
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3500
PvuII CAG'CTG
Cuts at: 925 1285 925
Size: 360 3140
RsrII CG'GwC_CG
Cuts at: 1695 1695
Size: 3500
SacI G_AGCT'C
Cuts at: 514 514
Size: 3500
SalI G'TCGA_C
Cuts at: 537 537
Size: 3500
SapI GCTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
SbfI CC_TGCA'GG
Cuts at: 547 547
Size: 3500
ScaI AGT'ACT
Cuts at: 3017 3017
Size: 3500
SfcI C'TryA_G
Cuts at: 33 414 543 1228 2221 2412 33
Size: 381 129 685 993 191 1121
Fragments arranged by size:
1121 993 685 381 191 129
SfoI GGC'GCC
Cuts at: 932 1180 932
Size: 248 3252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
SmaI CCC'GGG
Cuts at: 400 522 400
Size: 122 3378
SmlI C'TyrA_G
Cuts at: 655 1570 2062 2324 655
Size: 915 492 262 1831
Fragments arranged by size:
1831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3500
SphI G_CATG'C
Cuts at: 553 1053 553
Size: 500 3000
StuI AGG'CCT
Cuts at: 385 385
Size: 3500
StyI C'CwwG_G
Cuts at: 634 988 634
Size: 354 3146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1083 1216 1675 1884 1083
Size: 133 459 209 2699
Fragments arranged by size:
2699 459 209 133
TatI w'GTAC_w
Cuts at: 3015 3015
Size: 3500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 456 488 3017 3049 456
Size: 32 2529 32 907
Fragments arranged by size:
2529 907 32 32
Tth111I GACn'n_nGTC
Cuts at: 1297 1297
Size: 3500
XbaI T'CTAG_A
Cuts at: 531 531
Size: 3500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 995 995
Size: 3500
XmaI C'CCGG_G
Cuts at: 398 520 398
Size: 122 3378
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI