(Circular) (Six-base) MAPSORT of: pF1KB7086.seq Check: 6617 from: 1 to: 3512 pF1KB7086 3512 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 550 550 Size: 3512 Acc65I G'GTAC_C Cuts at: 344 528 344 Size: 184 3328 AclI AA'CG_TT Cuts at: 3352 3352 Size: 3512 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1337 1769 2516 2784 1337 Size: 432 747 268 2065 Fragments arranged by size: 2065 747 432 268 AflII C'TTAA_G Cuts at: 364 364 Size: 3512 AflIII A'CryG_T Cuts at: 864 1968 2945 864 Size: 1104 977 1431 Fragments arranged by size: 1431 1104 977 AgeI A'CCGG_T Cuts at: 1900 1900 Size: 3512 AlwNI CAG_nnn'CTG Cuts at: 817 2384 817 Size: 1567 1945 ApaLI G'TGCA_C Cuts at: 2282 2282 Size: 3512 ApoI r'AATT_y Cuts at: 516 2865 3121 516 Size: 2349 256 907 Fragments arranged by size: 2349 907 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3512 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3512 AvaI C'yCGr_G Cuts at: 532 532 Size: 3512 BamHI G'GATC_C Cuts at: 537 537 Size: 3512 BanI G'GyrC_C Cuts at: 344 528 942 1190 1225 344 Size: 184 414 248 35 2631 Fragments arranged by size: 2631 414 248 184 35 BanII G_rGCy'C Cuts at: 526 1556 526 Size: 1030 2482 BbeI G_GCGC'C Cuts at: 946 1194 946 Size: 248 3264 BbsI GAAGACnn'nnnn_ Cuts at: 426 426 Size: 3512 BciVI GTATCCnnnnn_n' Cuts at: 1410 2171 3512 1410 Size: 761 1341 1410 Fragments arranged by size: 1410 1341 761 BclI T'GATC_A Cuts at: 167 1032 167 Size: 865 2647 BglI GCCn_nnn'nGGC Cuts at: 457 457 Size: 3512 BglII A'GATC_T Cuts at: 1027 3064 1027 Size: 2037 1475 BlpI GC'TnA_GC Cuts at: 624 624 Size: 3512 Bme1580I G_kGCm'C Cuts at: 1137 1230 2286 1137 Size: 93 1056 2363 Fragments arranged by size: 2363 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 724 805 1140 3284 724 Size: 81 335 2144 952 Fragments arranged by size: 2144 952 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 154 1879 154 Size: 1725 1787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 688 1567 2059 2357 688 Size: 879 492 298 1843 Fragments arranged by size: 1843 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3216 3216 Size: 3512 BsaAI yAC'GTr Cuts at: 865 865 Size: 3512 BsaBI GATnn'nnATC Cuts at: 6 1026 3063 6 Size: 1020 2037 455 Fragments arranged by size: 2037 1020 455 BsaHI Gr'CG_yC Cuts at: 943 1191 1893 3389 943 Size: 248 702 1496 1066 Fragments arranged by size: 1496 1066 702 248 BsaWI w'CCGG_w Cuts at: 701 1222 1900 2174 2321 2732 701 Size: 521 678 274 147 411 1481 Fragments arranged by size: 1481 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 280 460 937 1695 2272 2768 280 Size: 180 477 758 577 496 1024 Fragments arranged by size: 1024 758 577 496 477 180 BsiEI CG_ry'CG Cuts at: 104 1054 1100 2308 104 Size: 950 46 1208 1308 Fragments arranged by size: 1308 1208 950 46 BsiHKAI G_wGCw'C Cuts at: 526 1304 1494 2286 526 Size: 778 190 792 1752 Fragments arranged by size: 1752 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 497 497 Size: 3512 Bsp1286I G_dGCh'C Cuts at: 526 1137 1230 1304 1494 1556 2286 526 Size: 611 93 74 190 62 730 1752 Fragments arranged by size: 1752 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2732 2732 Size: 3512 BspHI T'CATG_A Cuts at: 2688 2688 Size: 3512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 562 776 1078 1459 562 Size: 214 302 381 2615 Fragments arranged by size: 2615 381 302 214 BsrBI CCG'CTC Cuts at: 50 1804 1858 3342 3507 50 Size: 1754 54 1484 165 55 Fragments arranged by size: 1754 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 350 362 1424 350 Size: 12 1062 2438 Fragments arranged by size: 2438 1062 12 BsrFI r'CCGG_y Cuts at: 1510 1691 1900 1510 Size: 181 209 3122 Fragments arranged by size: 3122 209 181 BssSI C'ACGA_G Cuts at: 1783 2141 1783 Size: 358 3154 BstAPI GCAn_nnn'nTGC Cuts at: 391 889 391 Size: 498 3014 BstBI TT'CG_AA Cuts at: 1873 1873 Size: 3512 BstYI r'GATC_y Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718 Size: 536 467 23 335 246 1012 86 12 Cuts at: 2718 3064 1 Size: 346 449 Fragments arranged by size: 1012 536 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1462 1643 1806 1462 Size: 181 163 3168 Fragments arranged by size: 3168 181 163 BtsI GCAGTG_nn' Cuts at: 755 755 Size: 3512 DraI TTT'AAA Cuts at: 511 511 Size: 3512 DraIII CAC_nnn'GTG Cuts at: 391 391 Size: 3512 DrdI GACnn_nn'nnGTC Cuts at: 1218 2076 1218 Size: 858 2654 EaeI y'GGCC_r Cuts at: 1097 1271 1662 1689 3034 1097 Size: 174 391 27 1345 1575 Fragments arranged by size: 1575 1345 391 174 27 EagI C'GGCC_G Cuts at: 1097 1097 Size: 3512 EarI CTCTTCn'nnn_ Cuts at: 166 1535 1745 166 Size: 1369 210 1933 Fragments arranged by size: 1933 1369 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1720 2030 2176 3322 1720 Size: 310 146 1146 1910 Fragments arranged by size: 1910 1146 310 146 EcoICRI GAG'CTC Cuts at: 524 524 Size: 3512 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1337 1769 2516 2784 1337 Size: 432 747 268 2065 Fragments arranged by size: 2065 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 651 651 Size: 3512 EcoRI G'AATT_C Cuts at: 516 516 Size: 3512 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 315 390 1531 315 Size: 75 1141 2296 Fragments arranged by size: 2296 1141 75 FspI TGC'GCA Cuts at: 1293 1293 Size: 3512 HaeII r_GCGC'y Cuts at: 946 1194 2216 3195 946 Size: 248 1022 979 1263 Fragments arranged by size: 1263 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 473 505 1354 1384 1386 1416 473 Size: 32 849 30 2 30 2569 Fragments arranged by size: 2569 849 32 30 30 2 HincII GTy'rAC Cuts at: 551 551 Size: 3512 KasI G'GCGC_C Cuts at: 942 1190 942 Size: 248 3264 KpnI G_GTAC'C Cuts at: 348 532 348 Size: 184 3328 MluI A'CGCG_T Cuts at: 2945 2945 Size: 3512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2183 2367 2183 Size: 184 3328 MscI TGG'CCA Cuts at: 1273 1273 Size: 3512 MslI CAynn'nnrTG Cuts at: 112 175 355 1501 1628 112 Size: 63 180 1146 127 1996 Fragments arranged by size: 1996 1146 180 127 63 MspA1I CmG'CkG Cuts at: 623 937 1297 2310 2555 623 Size: 314 360 1013 245 1580 Fragments arranged by size: 1580 1013 360 314 245 NaeI GCC'GGC Cuts at: 1693 1693 Size: 3512 NarI GG'CG_CC Cuts at: 943 1191 943 Size: 248 3264 NgoMIV G'CCGG_C Cuts at: 1691 1691 Size: 3512 NspI r_CATG'y Cuts at: 565 1065 1972 565 Size: 500 907 2105 Fragments arranged by size: 2105 907 500 PciI A'CATG_T Cuts at: 1968 1968 Size: 3512 PmeI GTTT'AAAC Cuts at: 511 511 Size: 3512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2651 2683 2651 Size: 32 3480 PstI C_TGCA'G Cuts at: 559 1244 559 Size: 685 2827 PvuI CG_AT'CG Cuts at: 104 104 Size: 3512 PvuII CAG'CTG Cuts at: 937 1297 937 Size: 360 3152 RsrII CG'GwC_CG Cuts at: 1707 1707 Size: 3512 SacI G_AGCT'C Cuts at: 526 526 Size: 3512 SalI G'TCGA_C Cuts at: 549 549 Size: 3512 SapI GCTCTTCn'nnn_ Cuts at: 1535 1745 1535 Size: 210 3302 SbfI CC_TGCA'GG Cuts at: 559 559 Size: 3512 ScaI AGT'ACT Cuts at: 134 3029 134 Size: 2895 617 SfcI C'TryA_G Cuts at: 33 555 1240 2233 2424 33 Size: 522 685 993 191 1121 Fragments arranged by size: 1121 993 685 522 191 SfoI GGC'GCC Cuts at: 944 1192 944 Size: 248 3264 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3512 SmaI CCC'GGG Cuts at: 534 534 Size: 3512 SmlI C'TyrA_G Cuts at: 364 667 1582 2074 2336 364 Size: 303 915 492 262 1540 Fragments arranged by size: 1540 915 492 303 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3512 SphI G_CATG'C Cuts at: 565 1065 565 Size: 500 3012 SspI AAT'ATT Cuts at: 327 327 Size: 3512 StyI C'CwwG_G Cuts at: 119 202 646 1000 119 Size: 83 444 354 2631 Fragments arranged by size: 2631 444 354 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1095 1228 1687 1896 1095 Size: 133 459 209 2711 Fragments arranged by size: 2711 459 209 133 TatI w'GTAC_w Cuts at: 132 3027 132 Size: 2895 617 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3029 3061 3029 Size: 32 3480 Tth111I GACn'n_nGTC Cuts at: 1309 1309 Size: 3512 XbaI T'CTAG_A Cuts at: 543 543 Size: 3512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1007 1007 Size: 3512 XmaI C'CCGG_G Cuts at: 532 532 Size: 3512 XmnI GAAnn'nnTTC Cuts at: 211 211 Size: 3512 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI