Restriction map for pF1KB7086
 (Circular) (Six-base) MAPSORT of: pF1KB7086.seq  Check: 6617  from: 1  to: 3512



pF1KB7086  3512 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    550     550 
   Size:       3512
 
Acc65I G'GTAC_C

Cuts at:    344     528     344 
   Size:        184    3328
 
AclI AA'CG_TT

Cuts at:   3352    3352 
   Size:       3512
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1337    1769    2516    2784    1337 
   Size:        432     747     268    2065

  Fragments arranged by size: 

                2065     747     432     268
 
AflII C'TTAA_G

Cuts at:    364     364 
   Size:       3512
 
AflIII A'CryG_T

Cuts at:    864    1968    2945     864 
   Size:       1104     977    1431

  Fragments arranged by size: 

                1431    1104     977
 
AgeI A'CCGG_T

Cuts at:   1900    1900 
   Size:       3512
 
AlwNI CAG_nnn'CTG

Cuts at:    817    2384     817 
   Size:       1567    1945
 
ApaLI G'TGCA_C

Cuts at:   2282    2282 
   Size:       3512
 
ApoI r'AATT_y

Cuts at:    516    2865    3121     516 
   Size:       2349     256     907

  Fragments arranged by size: 

                2349     907     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3512
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3512
 
AvaI C'yCGr_G

Cuts at:    532     532 
   Size:       3512
 
BamHI G'GATC_C

Cuts at:    537     537 
   Size:       3512
 
BanI G'GyrC_C

Cuts at:    344     528     942    1190    1225     344 
   Size:        184     414     248      35    2631

  Fragments arranged by size: 

                2631     414     248     184      35
 
BanII G_rGCy'C

Cuts at:    526    1556     526 
   Size:       1030    2482
 
BbeI G_GCGC'C

Cuts at:    946    1194     946 
   Size:        248    3264
 
BbsI GAAGACnn'nnnn_

Cuts at:    426     426 
   Size:       3512
 
BciVI GTATCCnnnnn_n'

Cuts at:   1410    2171    3512    1410 
   Size:        761    1341    1410

  Fragments arranged by size: 

                1410    1341     761
 
BclI T'GATC_A

Cuts at:    167    1032     167 
   Size:        865    2647
 
BglI GCCn_nnn'nGGC

Cuts at:    457     457 
   Size:       3512
 
BglII A'GATC_T

Cuts at:   1027    3064    1027 
   Size:       2037    1475
 
BlpI GC'TnA_GC

Cuts at:    624     624 
   Size:       3512
 
Bme1580I G_kGCm'C

Cuts at:   1137    1230    2286    1137 
   Size:         93    1056    2363

  Fragments arranged by size: 

                2363    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    724     805    1140    3284     724 
   Size:         81     335    2144     952

  Fragments arranged by size: 

                2144     952     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    154    1879     154 
   Size:       1725    1787
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    688    1567    2059    2357     688 
   Size:        879     492     298    1843

  Fragments arranged by size: 

                1843     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3216    3216 
   Size:       3512
 
BsaAI yAC'GTr

Cuts at:    865     865 
   Size:       3512
 
BsaBI GATnn'nnATC

Cuts at:      6    1026    3063       6 
   Size:       1020    2037     455

  Fragments arranged by size: 

                2037    1020     455
 
BsaHI Gr'CG_yC

Cuts at:    943    1191    1893    3389     943 
   Size:        248     702    1496    1066

  Fragments arranged by size: 

                1496    1066     702     248
 
BsaWI w'CCGG_w

Cuts at:    701    1222    1900    2174    2321    2732     701 
   Size:        521     678     274     147     411    1481

  Fragments arranged by size: 

                1481     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    280     460     937    1695    2272    2768     280 
   Size:        180     477     758     577     496    1024

  Fragments arranged by size: 

                1024     758     577     496     477     180
 
BsiEI CG_ry'CG

Cuts at:    104    1054    1100    2308     104 
   Size:        950      46    1208    1308

  Fragments arranged by size: 

                1308    1208     950      46
 
BsiHKAI G_wGCw'C

Cuts at:    526    1304    1494    2286     526 
   Size:        778     190     792    1752

  Fragments arranged by size: 

                1752     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    497     497 
   Size:       3512
 
Bsp1286I G_dGCh'C

Cuts at:    526    1137    1230    1304    1494    1556    2286     526 
   Size:        611      93      74     190      62     730    1752

  Fragments arranged by size: 

                1752     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2732    2732 
   Size:       3512
 
BspHI T'CATG_A

Cuts at:   2688    2688 
   Size:       3512
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    562     776    1078    1459     562 
   Size:        214     302     381    2615

  Fragments arranged by size: 

                2615     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1804    1858    3342    3507      50 
   Size:       1754      54    1484     165      55

  Fragments arranged by size: 

                1754    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    350     362    1424     350 
   Size:         12    1062    2438

  Fragments arranged by size: 

                2438    1062      12
 
BsrFI r'CCGG_y

Cuts at:   1510    1691    1900    1510 
   Size:        181     209    3122

  Fragments arranged by size: 

                3122     209     181
 
BssSI C'ACGA_G

Cuts at:   1783    2141    1783 
   Size:        358    3154
 
BstAPI GCAn_nnn'nTGC

Cuts at:    391     889     391 
   Size:        498    3014
 
BstBI TT'CG_AA

Cuts at:   1873    1873 
   Size:       3512
 
BstYI r'GATC_y

Cuts at:      1     537    1004    1027    1362    1608    2620    2706    2718 
   Size:        536     467      23     335     246    1012      86      12

Cuts at:   2718    3064       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     536     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1462    1643    1806    1462 
   Size:        181     163    3168

  Fragments arranged by size: 

                3168     181     163
 
BtsI GCAGTG_nn'

Cuts at:    755     755 
   Size:       3512
 
DraI TTT'AAA

Cuts at:    511     511 
   Size:       3512
 
DraIII CAC_nnn'GTG

Cuts at:    391     391 
   Size:       3512
 
DrdI GACnn_nn'nnGTC

Cuts at:   1218    2076    1218 
   Size:        858    2654
 
EaeI y'GGCC_r

Cuts at:   1097    1271    1662    1689    3034    1097 
   Size:        174     391      27    1345    1575

  Fragments arranged by size: 

                1575    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1097    1097 
   Size:       3512
 
EarI CTCTTCn'nnn_

Cuts at:    166    1535    1745     166 
   Size:       1369     210    1933

  Fragments arranged by size: 

                1933    1369     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1720    2030    2176    3322    1720 
   Size:        310     146    1146    1910

  Fragments arranged by size: 

                1910    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    524     524 
   Size:       3512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1337    1769    2516    2784    1337 
   Size:        432     747     268    2065

  Fragments arranged by size: 

                2065     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    651     651 
   Size:       3512
 
EcoRI G'AATT_C

Cuts at:    516     516 
   Size:       3512
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    315     390    1531     315 
   Size:         75    1141    2296

  Fragments arranged by size: 

                2296    1141      75
 
FspI TGC'GCA

Cuts at:   1293    1293 
   Size:       3512
 
HaeII r_GCGC'y

Cuts at:    946    1194    2216    3195     946 
   Size:        248    1022     979    1263

  Fragments arranged by size: 

                1263    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    473     505    1354    1384    1386    1416     473 
   Size:         32     849      30       2      30    2569

  Fragments arranged by size: 

                2569     849      32      30      30       2
 
HincII GTy'rAC

Cuts at:    551     551 
   Size:       3512
 
KasI G'GCGC_C

Cuts at:    942    1190     942 
   Size:        248    3264
 
KpnI G_GTAC'C

Cuts at:    348     532     348 
   Size:        184    3328
 
MluI A'CGCG_T

Cuts at:   2945    2945 
   Size:       3512
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2183    2367    2183 
   Size:        184    3328
 
MscI TGG'CCA

Cuts at:   1273    1273 
   Size:       3512
 
MslI CAynn'nnrTG

Cuts at:    112     175     355    1501    1628     112 
   Size:         63     180    1146     127    1996

  Fragments arranged by size: 

                1996    1146     180     127      63
 
MspA1I CmG'CkG

Cuts at:    623     937    1297    2310    2555     623 
   Size:        314     360    1013     245    1580

  Fragments arranged by size: 

                1580    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1693    1693 
   Size:       3512
 
NarI GG'CG_CC

Cuts at:    943    1191     943 
   Size:        248    3264
 
NgoMIV G'CCGG_C

Cuts at:   1691    1691 
   Size:       3512
 
NspI r_CATG'y

Cuts at:    565    1065    1972     565 
   Size:        500     907    2105

  Fragments arranged by size: 

                2105     907     500
 
PciI A'CATG_T

Cuts at:   1968    1968 
   Size:       3512
 
PmeI GTTT'AAAC

Cuts at:    511     511 
   Size:       3512
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2651    2683    2651 
   Size:         32    3480
 
PstI C_TGCA'G

Cuts at:    559    1244     559 
   Size:        685    2827
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3512
 
PvuII CAG'CTG

Cuts at:    937    1297     937 
   Size:        360    3152
 
RsrII CG'GwC_CG

Cuts at:   1707    1707 
   Size:       3512
 
SacI G_AGCT'C

Cuts at:    526     526 
   Size:       3512
 
SalI G'TCGA_C

Cuts at:    549     549 
   Size:       3512
 
SapI GCTCTTCn'nnn_

Cuts at:   1535    1745    1535 
   Size:        210    3302
 
SbfI CC_TGCA'GG

Cuts at:    559     559 
   Size:       3512
 
ScaI AGT'ACT

Cuts at:    134    3029     134 
   Size:       2895     617
 
SfcI C'TryA_G

Cuts at:     33     555    1240    2233    2424      33 
   Size:        522     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     522     191
 
SfoI GGC'GCC

Cuts at:    944    1192     944 
   Size:        248    3264
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3512
 
SmaI CCC'GGG

Cuts at:    534     534 
   Size:       3512
 
SmlI C'TyrA_G

Cuts at:    364     667    1582    2074    2336     364 
   Size:        303     915     492     262    1540

  Fragments arranged by size: 

                1540     915     492     303     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3512
 
SphI G_CATG'C

Cuts at:    565    1065     565 
   Size:        500    3012
 
SspI AAT'ATT

Cuts at:    327     327 
   Size:       3512
 
StyI C'CwwG_G

Cuts at:    119     202     646    1000     119 
   Size:         83     444     354    2631

  Fragments arranged by size: 

                2631     444     354      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1095    1228    1687    1896    1095 
   Size:        133     459     209    2711

  Fragments arranged by size: 

                2711     459     209     133
 
TatI w'GTAC_w

Cuts at:    132    3027     132 
   Size:       2895     617
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3029    3061    3029 
   Size:         32    3480
 
Tth111I GACn'n_nGTC

Cuts at:   1309    1309 
   Size:       3512
 
XbaI T'CTAG_A

Cuts at:    543     543 
   Size:       3512
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1007    1007 
   Size:       3512
 
XmaI C'CCGG_G

Cuts at:    532     532 
   Size:       3512
 
XmnI GAAnn'nnTTC

Cuts at:    211     211 
   Size:       3512
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     ZraI
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