Restriction map for
pF1KB7087
(Circular) (Six-base) MAPSORT of: pF1KB7087.seq Check: 4547 from: 1 to: 3515
pF1KB7087 3515 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 250 531 250
Size: 281 3234
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 1340 1772 2519 2787 134
Size: 1206 432 747 268 862
Fragments arranged by size:
1206 862 747 432 268
AflIII A'CryG_T
Cuts at: 867 1971 2948 867
Size: 1104 977 1434
Fragments arranged by size:
1434 1104 977
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AleI CACnn'nnGTG
Cuts at: 325 325
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 820 2387 820
Size: 1567 1948
ApaLI G'TGCA_C
Cuts at: 2285 2285
Size: 3515
ApoI r'AATT_y
Cuts at: 519 2868 3124 519
Size: 2349 256 910
Fragments arranged by size:
2349 910 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 535 535
Size: 3515
BamHI G'GATC_C
Cuts at: 540 540
Size: 3515
BanI G'GyrC_C
Cuts at: 250 531 945 1193 1228 250
Size: 281 414 248 35 2537
Fragments arranged by size:
2537 414 281 248 35
BanII G_rGCy'C
Cuts at: 122 144 181 260 529 1559 122
Size: 22 37 79 269 1030 2078
Fragments arranged by size:
2078 1030 269 79 37 22
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BbsI GAAGACnn'nnnn_
Cuts at: 291 291
Size: 3515
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2174 3515 1413
Size: 761 1341 1413
Fragments arranged by size:
1413 1341 761
BclI T'GATC_A
Cuts at: 197 1035 197
Size: 838 2677
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 3515
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 627 627
Size: 3515
Bme1580I G_kGCm'C
Cuts at: 1140 1233 2289 1140
Size: 93 1056 2366
Fragments arranged by size:
2366 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 396
Size: 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1570 2062 2360 691
Size: 879 492 298 1846
Fragments arranged by size:
1846 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 3515
BsaAI yAC'GTr
Cuts at: 868 868
Size: 3515
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 946 1194 1896 3392 946
Size: 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 704 1225 1903 2177 2324 2735 704
Size: 521 678 274 147 411 1484
Fragments arranged by size:
1484 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 940 1698 2275 2771 940
Size: 758 577 496 1684
Fragments arranged by size:
1684 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1057 1103 2311 104
Size: 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 260 529 1307 1497 2289 260
Size: 269 778 190 792 1486
Fragments arranged by size:
1486 792 778 269 190
BsmBI CGTCTCn'nnnn_
Cuts at: 453 453
Size: 3515
Bsp1286I G_dGCh'C
Cuts at: 122 144 181 260 529 1140 1233 1307 1497
Size: 22 37 79 269 611 93 74 190
Cuts at: 1497 1559 2289 122
Size: 62 730 1348
Fragments arranged by size:
1348 730 611 269 190 93 79 74
62 37 22
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 779 1081 1462 565
Size: 214 302 381 2618
Fragments arranged by size:
2618 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1807 1861 3345 3510 50
Size: 1757 54 1484 165 55
Fragments arranged by size:
1757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 307 1513 1694 1903 307
Size: 1206 181 209 1919
Fragments arranged by size:
1919 1206 209 181
BssSI C'ACGA_G
Cuts at: 481 1786 2144 481
Size: 1305 358 1852
Fragments arranged by size:
1852 1305 358
BstAPI GCAn_nnn'nTGC
Cuts at: 892 892
Size: 3515
BstBI TT'CG_AA
Cuts at: 1876 1876
Size: 3515
BstEII G'GTnAC_C
Cuts at: 318 318
Size: 3515
BstYI r'GATC_y
Cuts at: 1 131 540 1007 1030 1365 1611 2623 2709
Size: 130 409 467 23 335 246 1012 86
Cuts at: 2709 2721 3067 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 409 346 335 246 130
86 23 12
BtgI C'CryG_G
Cuts at: 323 348 323
Size: 25 3490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 288 1465 1646 1809 288
Size: 1177 181 163 1994
Fragments arranged by size:
1994 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 758 758
Size: 3515
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 467 1221 2079 467
Size: 754 858 1903
Fragments arranged by size:
1903 858 754
EaeI y'GGCC_r
Cuts at: 1100 1274 1665 1692 3037 1100
Size: 174 391 27 1345 1578
Fragments arranged by size:
1578 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1100 1100
Size: 3515
EarI CTCTTCn'nnn_
Cuts at: 110 169 1538 1748 110
Size: 59 1369 210 1877
Fragments arranged by size:
1877 1369 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1723 2033 2179 3325 1723
Size: 310 146 1146 1913
Fragments arranged by size:
1913 1146 310 146
EcoICRI GAG'CTC
Cuts at: 258 527 258
Size: 269 3246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 396 1340 1772 2519 2787 134
Size: 262 944 432 747 268 862
Fragments arranged by size:
944 862 747 432 268 262
EcoO109I rG'GnC_Cy
Cuts at: 654 654
Size: 3515
EcoRI G'AATT_C
Cuts at: 519 519
Size: 3515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1534 1534
Size: 3515
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 949 1197 2219 3198 949
Size: 248 1022 979 1266
Fragments arranged by size:
1266 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1357 1387 1389 1419 1357
Size: 30 2 30 3453
Fragments arranged by size:
3453 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
HindIII A'AGCT_T
Cuts at: 498 498
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 254 535 254
Size: 281 3234
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2370 2186
Size: 184 3331
MscI TGG'CCA
Cuts at: 1276 1276
Size: 3515
MslI CAynn'nnrTG
Cuts at: 325 1504 1631 325
Size: 1179 127 2209
Fragments arranged by size:
2209 1179 127
MspA1I CmG'CkG
Cuts at: 272 325 626 940 1300 2313 2558 272
Size: 53 301 314 360 1013 245 1229
Fragments arranged by size:
1229 1013 360 314 301 245 53
NaeI GCC'GGC
Cuts at: 309 1696 309
Size: 1387 2128
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NcoI C'CATG_G
Cuts at: 348 348
Size: 3515
NgoMIV G'CCGG_C
Cuts at: 307 1694 307
Size: 1387 2128
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PstI C_TGCA'G
Cuts at: 562 1247 562
Size: 685 2830
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 940 1300 940
Size: 360 3155
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 260 529 260
Size: 269 3246
SacII CC_GC'GG
Cuts at: 326 326
Size: 3515
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 110 169 1538 1748 110
Size: 59 1369 210 1877
Fragments arranged by size:
1877 1369 210 59
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 242 558 1243 2236 2427 33
Size: 209 316 685 993 191 1121
Fragments arranged by size:
1121 993 685 316 209 191
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 670 1585 2077 2339 670
Size: 915 492 262 1846
Fragments arranged by size:
1846 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
StyI C'CwwG_G
Cuts at: 348 649 1003 348
Size: 301 354 2860
Fragments arranged by size:
2860 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1231 1690 1899 1098
Size: 133 459 209 2714
Fragments arranged by size:
2714 459 209 133
TatI w'GTAC_w
Cuts at: 3030 3030
Size: 3515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3483
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 546 546
Size: 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 372 1010 210
Size: 162 638 2715
Fragments arranged by size:
2715 638 162
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI