Restriction map for
pF1KB7090
(Circular) (Six-base) MAPSORT of: pF1KB7090.seq Check: 2184 from: 1 to: 3623
pF1KB7090 3623 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 387
Size: 3623
AccI GT'mk_AC
Cuts at: 661 661
Size: 3623
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2627 2895 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 747 432 268
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 928 2495 928
Size: 1567 2056
ApaLI G'TGCA_C
Cuts at: 508 2393 508
Size: 1885 1738
ApoI r'AATT_y
Cuts at: 612 627 2976 3232 612
Size: 15 2349 256 1003
Fragments arranged by size:
2349 1003 256 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 643 643
Size: 3623
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 145 399 435 639 1053 1301 1336 145
Size: 254 36 204 414 248 35 2432
Fragments arranged by size:
2432 414 254 248 204 36 35
BanII G_rGCy'C
Cuts at: 487 637 1667 487
Size: 150 1030 2443
Fragments arranged by size:
2443 1030 150
BbeI G_GCGC'C
Cuts at: 439 1057 1305 439
Size: 618 248 2757
Fragments arranged by size:
2757 618 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3589
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 735 735
Size: 3623
Bme1580I G_kGCm'C
Cuts at: 404 512 1248 1341 2397 404
Size: 108 736 93 1056 1630
Fragments arranged by size:
1630 1056 736 108 93
BmgBI CAC'GTC
Cuts at: 507 507
Size: 3623
BmrI ACTGGGnnnn_n'
Cuts at: 835 916 1251 3395 835
Size: 81 335 2144 1063
Fragments arranged by size:
2144 1063 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 573 1990 513
Size: 60 1417 2146
Fragments arranged by size:
2146 1417 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 190 568 141
Size: 49 378 3196
Fragments arranged by size:
3196 378 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 3327 547
Size: 2780 843
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 262 1137 3174 6
Size: 256 875 2037 455
Fragments arranged by size:
2037 875 455 256
BsaHI Gr'CG_yC
Cuts at: 436 565 1054 1302 2004 3500 436
Size: 129 489 248 702 1496 559
Fragments arranged by size:
1496 702 559 489 248 129
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2285 2432 2843 812
Size: 521 678 274 147 411 1592
Fragments arranged by size:
1592 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 3593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 349 486 541 556 565 349
Size: 137 55 15 9 3407
Fragments arranged by size:
3407 137 55 15 9
BseYI C'CCAG_C
Cuts at: 270 1048 1806 2383 2879 270
Size: 778 758 577 496 1014
Fragments arranged by size:
1014 778 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 435 415
Size: 20 3603
BsiEI CG_ry'CG
Cuts at: 104 185 1165 1211 2419 104
Size: 81 980 46 1208 1308
Fragments arranged by size:
1308 1208 980 81 46
BsiHKAI G_wGCw'C
Cuts at: 487 512 637 1415 1605 2397 487
Size: 25 125 778 190 792 1713
Fragments arranged by size:
1713 792 778 190 125 25
BsmBI CGTCTCn'nnnn_
Cuts at: 556 556
Size: 3623
Bsp1286I G_dGCh'C
Cuts at: 404 487 512 637 1248 1341 1415 1605 1667
Size: 83 25 125 611 93 74 190 62
Cuts at: 1667 2397 404
Size: 730 1630
Fragments arranged by size:
1630 730 611 190 125 93 83 74
62 25
BspEI T'CCGG_A
Cuts at: 2843 2843
Size: 3623
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 387 673 887 1189 1570 387
Size: 286 214 302 381 2440
Fragments arranged by size:
2440 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 339 1915 1969 3453 3618 50
Size: 289 1576 54 1484 165 55
Fragments arranged by size:
1576 1484 289 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 1535 207
Size: 1328 2295
BsrFI r'CCGG_y
Cuts at: 184 1621 1802 2011 184
Size: 1437 181 209 1796
Fragments arranged by size:
1796 1437 209 181
BsrGI T'GTAC_A
Cuts at: 323 323
Size: 3623
BssSI C'ACGA_G
Cuts at: 1894 2252 1894
Size: 358 3265
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstYI r'GATC_y
Cuts at: 1 648 1115 1138 1473 1719 2731 2817 2829
Size: 647 467 23 335 246 1012 86 12
Cuts at: 2829 3175 1
Size: 346 449
Fragments arranged by size:
1012 647 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 385 385
Size: 3623
BtgI C'CryG_G
Cuts at: 371 464 371
Size: 93 3530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 866 866
Size: 3623
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 182 1208 1382 1773 1800 3145 182
Size: 1026 174 391 27 1345 660
Fragments arranged by size:
1345 1026 660 391 174 27
EagI C'GGCC_G
Cuts at: 182 1208 182
Size: 1026 2597
EarI CTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2141 2287 3433 1831
Size: 310 146 1146 2021
Fragments arranged by size:
2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 485 635 485
Size: 150 3473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 190 568 1448 1880 2627 2895 141
Size: 49 378 880 432 747 268 869
Fragments arranged by size:
880 869 747 432 378 268 49
EcoO109I rG'GnC_Cy
Cuts at: 590 762 590
Size: 172 3451
EcoRI G'AATT_C
Cuts at: 612 627 612
Size: 15 3608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1642 1642
Size: 3623
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 439 1057 1305 2327 3306 439
Size: 618 248 1022 979 756
Fragments arranged by size:
1022 979 756 618 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 1465 1495 1497 1527 289
Size: 32 1144 30 2 30 2385
Fragments arranged by size:
2385 1144 32 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
KasI G'GCGC_C
Cuts at: 435 1053 1301 435
Size: 618 248 2757
Fragments arranged by size:
2757 618 248
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 1384 1384
Size: 3623
MslI CAynn'nnrTG
Cuts at: 1612 1739 1612
Size: 127 3496
MspA1I CmG'CkG
Cuts at: 197 366 734 1048 1408 2421 2666 197
Size: 169 368 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 368 360 314 245 169
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 436 1054 1302 436
Size: 618 248 2757
Fragments arranged by size:
2757 618 248
NcoI C'CATG_G
Cuts at: 464 464
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NspI r_CATG'y
Cuts at: 420 676 1176 2083 420
Size: 256 500 907 1960
Fragments arranged by size:
1960 907 500 256
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 3591
PpuMI rG'GwC_Cy
Cuts at: 590 590
Size: 3623
PstI C_TGCA'G
Cuts at: 425 670 1355 425
Size: 245 685 2693
Fragments arranged by size:
2693 685 245
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 1048 1408 1048
Size: 360 3263
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 487 637 487
Size: 150 3473
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 214 3140 214
Size: 2926 697
SfcI C'TryA_G
Cuts at: 33 421 666 1351 2344 2535 33
Size: 388 245 685 993 191 1121
Fragments arranged by size:
1121 993 685 388 245 191
SfoI GGC'GCC
Cuts at: 437 1055 1303 437
Size: 618 248 2757
Fragments arranged by size:
2757 618 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 778 1693 2185 2447 778
Size: 915 492 262 1954
Fragments arranged by size:
1954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 464 757 1111 464
Size: 293 354 2976
Fragments arranged by size:
2976 354 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 212 323 3138 212
Size: 111 2815 697
Fragments arranged by size:
2815 697 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 165 1118 114
Size: 51 953 2619
Fragments arranged by size:
2619 953 51
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI