Restriction map for
pF1KB7094
(Circular) (Six-base) MAPSORT of: pF1KB7094.seq Check: 9436 from: 1 to: 3671
pF1KB7094 3671 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 623 623
Size: 3671
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 331 1496 1928 2675 2943 102
Size: 229 1165 432 747 268 830
Fragments arranged by size:
1165 830 747 432 268 229
AflIII A'CryG_T
Cuts at: 542 1023 2127 3104 542
Size: 481 1104 977 1109
Fragments arranged by size:
1109 1104 977 481
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 626 976 2543 626
Size: 350 1567 1754
Fragments arranged by size:
1754 1567 350
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 675 3024 3280 675
Size: 2349 256 1066
Fragments arranged by size:
2349 1066 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 469 691 469
Size: 222 3449
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 602 685 1715 602
Size: 83 1030 2558
Fragments arranged by size:
2558 1030 83
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglI GCCn_nnn'nGGC
Cuts at: 406 406
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2445 1296
Size: 93 1056 2522
Fragments arranged by size:
2522 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 152 2038 152
Size: 1886 1785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 847 1726 2218 2516 271
Size: 576 879 492 298 1426
Fragments arranged by size:
1426 879 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 543 1024 543
Size: 481 3190
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 172 1102 1350 2052 3548 172
Size: 930 248 702 1496 295
Fragments arranged by size:
1496 930 702 295 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 367
Size: 3671
BseYI C'CCAG_C
Cuts at: 315 342 1096 1854 2431 2927 315
Size: 27 754 758 577 496 1059
Fragments arranged by size:
1059 758 754 577 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 3671
BsiEI CG_ry'CG
Cuts at: 104 410 444 531 1213 1259 2467 104
Size: 306 34 87 682 46 1208 1308
Fragments arranged by size:
1308 1208 682 306 87 46 34
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 602 685 1296 1389 1463 1653 1715 2445 602
Size: 83 611 93 74 190 62 730 1828
Fragments arranged by size:
1828 730 611 190 93 83 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 623 721 935 1237 1618 491
Size: 132 98 214 302 381 2544
Fragments arranged by size:
2544 381 302 214 132 98
BsrBI CCG'CTC
Cuts at: 50 608 1963 2017 3501 3666 50
Size: 558 1355 54 1484 165 55
Fragments arranged by size:
1484 1355 558 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 443 1669 1850 2059 443
Size: 1226 181 209 2055
Fragments arranged by size:
2055 1226 209 181
BssHII G'CGCG_C
Cuts at: 388 388
Size: 3671
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 626 1048 626
Size: 422 3249
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstXI CCAn_nnnn'nTGG
Cuts at: 132 183 132
Size: 51 3620
BstYI r'GATC_y
Cuts at: 1 623 696 1163 1186 1521 1767 2779 2865
Size: 622 73 467 23 335 246 1012 86
Cuts at: 2865 2877 3223 1
Size: 12 346 449
Fragments arranged by size:
1012 622 467 449 346 335 246 86
73 23 12
BtgI C'CryG_G
Cuts at: 107 497 525 107
Size: 390 28 3253
Fragments arranged by size:
3253 390 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 188 219 914 188
Size: 31 695 2945
Fragments arranged by size:
2945 695 31
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DraIII CAC_nnn'GTG
Cuts at: 426 426
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 519 1377 2235 519
Size: 858 858 1955
Fragments arranged by size:
1955 858 858
EaeI y'GGCC_r
Cuts at: 188 398 407 455 528 549 1256 1430 1821
Size: 210 9 48 73 21 707 174 391
Cuts at: 1821 1848 3193 188
Size: 27 1345 666
Fragments arranged by size:
1345 707 666 391 210 174 73 48
27 21 9
EagI C'GGCC_G
Cuts at: 407 528 1256 407
Size: 121 728 2822
Fragments arranged by size:
2822 728 121
EarI CTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 367 1879 2189 2335 3481 367
Size: 1512 310 146 1146 557
Fragments arranged by size:
1512 1146 557 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 331 1496 1928 2675 2943 102
Size: 229 1165 432 747 268 830
Fragments arranged by size:
1165 830 747 432 268 229
EcoNI CCTnn'n_nnAGG
Cuts at: 298 298
Size: 3671
EcoO109I rG'GnC_Cy
Cuts at: 502 633 810 502
Size: 131 177 3363
Fragments arranged by size:
3363 177 131
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 619 1452 619
Size: 833 2838
HaeII r_GCGC'y
Cuts at: 364 375 1105 1353 2375 3354 364
Size: 11 730 248 1022 979 681
Fragments arranged by size:
1022 979 730 681 248 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 400 1432 400
Size: 1032 2639
MslI CAynn'nnrTG
Cuts at: 1660 1787 1660
Size: 127 3544
MspA1I CmG'CkG
Cuts at: 342 782 1096 1456 2469 2714 342
Size: 440 314 360 1013 245 1299
Fragments arranged by size:
1299 1013 440 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 724 1224 2131 724
Size: 500 907 2264
Fragments arranged by size:
2264 907 500
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 426 498 426
Size: 72 3599
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PmlI CAC'GTG
Cuts at: 543 543
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PpuMI rG'GwC_Cy
Cuts at: 502 502
Size: 3671
PshAI GACnn'nnGTC
Cuts at: 409 409
Size: 3671
PspXI vC'TCGA_Gb
Cuts at: 469 469
Size: 3671
PstI C_TGCA'G
Cuts at: 143 718 1403 143
Size: 575 685 2411
Fragments arranged by size:
2411 685 575
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 342 1096 1456 342
Size: 754 360 2557
Fragments arranged by size:
2557 754 360
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SanDI GG'GwC_CC
Cuts at: 502 502
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SexAI A'CCwGG_T
Cuts at: 293 422 293
Size: 129 3542
SfcI C'TryA_G
Cuts at: 33 139 714 1399 2392 2583 33
Size: 106 575 685 993 191 1121
Fragments arranged by size:
1121 993 685 575 191 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 406
Size: 3671
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 286 469 826 1741 2233 2495 286
Size: 183 357 915 492 262 1462
Fragments arranged by size:
1462 915 492 357 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 107 176 805 1159 107
Size: 69 629 354 2619
Fragments arranged by size:
2619 629 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1166 183
Size: 983 2688
XhoI C'TCGA_G
Cuts at: 469 469
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PsiI PspOMI PsrI SacII SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI