Restriction map for
pF1KB7096
(Circular) (Six-base) MAPSORT of: pF1KB7096.seq Check: 6417 from: 1 to: 3692
pF1KB7096 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 308 730 308
Size: 422 3270
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1949 2696 2964 1517
Size: 432 747 268 2245
Fragments arranged by size:
2245 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 585 712 585
Size: 127 3565
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 162 411 533 575 708 1122 1370 1405 162
Size: 249 122 42 133 414 248 35 2449
Fragments arranged by size:
2449 414 249 248 133 122 42 35
BanII G_rGCy'C
Cuts at: 173 706 1736 173
Size: 533 1030 2129
Fragments arranged by size:
2129 1030 533
BbeI G_GCGC'C
Cuts at: 166 579 1126 1374 166
Size: 413 547 248 2484
Fragments arranged by size:
2484 547 413 248
BciVI GTATCCnnnnn_n'
Cuts at: 239 1590 2351 3692 239
Size: 1351 761 1341 239
Fragments arranged by size:
1351 1341 761 239
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 677 804 677
Size: 127 3565
Bme1580I G_kGCm'C
Cuts at: 1317 1410 2466 1317
Size: 93 1056 2543
Fragments arranged by size:
2543 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 904 985 1320 3464 904
Size: 81 335 2144 1132
Fragments arranged by size:
2144 1132 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 500 2059 500
Size: 1559 2133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 445 495 550 625 353
Size: 92 50 55 75 3420
Fragments arranged by size:
3420 92 75 55 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1747 2239 2537 868
Size: 879 492 298 2023
Fragments arranged by size:
2023 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 163 576 1123 1371 2073 3569 163
Size: 413 547 248 702 1496 286
Fragments arranged by size:
1496 702 547 413 286 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 443 473 443
Size: 30 3662
BseYI C'CCAG_C
Cuts at: 173 1117 1875 2452 2948 173
Size: 944 758 577 496 917
Fragments arranged by size:
944 917 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 346 706 1484 1674 2466 346
Size: 360 778 190 792 1572
Fragments arranged by size:
1572 792 778 360 190
Bsp1286I G_dGCh'C
Cuts at: 173 346 706 1317 1410 1484 1674 1736 2466
Size: 173 360 611 93 74 190 62 730
Cuts at: 2466 173
Size: 1399
Fragments arranged by size:
1399 730 611 360 190 173 93 74
62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 665 717 1184 1207 1542 1788 2800 2886
Size: 664 52 467 23 335 246 1012 86
Cuts at: 2886 2898 3244 1
Size: 12 346 449
Fragments arranged by size:
1012 664 467 449 346 335 246 86
52 23 12
BtgI C'CryG_G
Cuts at: 107 211 571 107
Size: 104 360 3228
Fragments arranged by size:
3228 360 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 517 1398 2256 517
Size: 881 858 1953
Fragments arranged by size:
1953 881 858
EaeI y'GGCC_r
Cuts at: 385 1277 1451 1842 1869 3214 385
Size: 892 174 391 27 1345 863
Fragments arranged by size:
1345 892 863 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 224 416 1715 1925 224
Size: 192 1299 210 1991
Fragments arranged by size:
1991 1299 210 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 445 495 550 625 1517 1949 2696 2964
Size: 92 50 55 75 892 432 747 268
Cuts at: 2964 353
Size: 1081
Fragments arranged by size:
1081 892 747 432 268 92 75 55
50
EcoO109I rG'GnC_Cy
Cuts at: 831 831
Size: 3692
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 166 579 1126 1374 2396 3375 166
Size: 413 547 248 1022 979 483
Fragments arranged by size:
1022 979 547 483 413 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 1534 1564 1566 1596 443
Size: 32 1059 30 2 30 2539
Fragments arranged by size:
2539 1059 32 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 162 575 1122 1370 162
Size: 413 547 248 2484
Fragments arranged by size:
2484 547 413 248
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2547 2363
Size: 184 3508
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 260 530 803 1117 1477 2490 2735 260
Size: 270 273 314 360 1013 245 1217
Fragments arranged by size:
1217 1013 360 314 273 270 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 163 576 1123 1371 163
Size: 413 547 248 2484
Fragments arranged by size:
2484 547 413 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PasI CC'CwG_GG
Cuts at: 494 494
Size: 3692
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PfoI T'CCnGG_A
Cuts at: 330 330
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 260 1117 1477 260
Size: 857 360 2475
Fragments arranged by size:
2475 857 360
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 236 247 3209 236
Size: 11 2962 719
Fragments arranged by size:
2962 719 11
SfcI C'TryA_G
Cuts at: 33 735 1420 2413 2604 33
Size: 702 685 993 191 1121
Fragments arranged by size:
1121 993 702 685 191
SfoI GGC'GCC
Cuts at: 164 577 1124 1372 164
Size: 413 547 248 2484
Fragments arranged by size:
2484 547 413 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 587 714 587
Size: 127 3565
SmlI C'TyrA_G
Cuts at: 847 1762 2254 2516 847
Size: 915 492 262 2023
Fragments arranged by size:
2023 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 107 347 826 1180 107
Size: 240 479 354 2619
Fragments arranged by size:
2619 479 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 234 245 358 642 3207 234
Size: 11 113 284 2565 719
Fragments arranged by size:
2565 719 284 113 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 585 712 585
Size: 127 3565
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI