Restriction map for
pF1KB7097
(Circular) (Six-base) MAPSORT of: pF1KB7097.seq Check: 2481 from: 1 to: 3695
pF1KB7097 3695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 484 484
Size: 3695
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
AflIII A'CryG_T
Cuts at: 205 1047 2151 3128 205
Size: 842 1104 977 772
Fragments arranged by size:
1104 977 842 772
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 699 3048 3304 699
Size: 2349 256 1090
Fragments arranged by size:
2349 1090 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 437 720 437
Size: 283 3412
BanI G'GyrC_C
Cuts at: 652 711 1125 1373 1408 652
Size: 59 414 248 35 2939
Fragments arranged by size:
2939 414 248 59 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 504 538 504
Size: 34 3661
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 657 1320 1413 2469 657
Size: 663 93 1056 1883
Fragments arranged by size:
1883 1056 663 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 408 3399 408
Size: 2991 704
BsaAI yAC'GTr
Cuts at: 126 1048 126
Size: 922 2773
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 471 574 675 471
Size: 103 101 3491
Fragments arranged by size:
3491 103 101
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 120 1237 1283 2491 104
Size: 16 1117 46 1208 1308
Fragments arranged by size:
1308 1208 1117 46 16
BsiHKAI G_wGCw'C
Cuts at: 669 709 1487 1677 2469 669
Size: 40 778 190 792 1895
Fragments arranged by size:
1895 792 778 190 40
Bsp1286I G_dGCh'C
Cuts at: 657 669 709 1320 1413 1487 1677 1739 2469
Size: 12 40 611 93 74 190 62 730
Cuts at: 2469 657
Size: 1883
Fragments arranged by size:
1883 730 611 190 93 74 62 40
12
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 161 2871 161
Size: 2710 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 745 959 1261 1642 484
Size: 261 214 302 381 2537
Fragments arranged by size:
2537 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 3695
BssSI C'ACGA_G
Cuts at: 269 1966 2324 269
Size: 1697 358 1640
Fragments arranged by size:
1697 1640 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 437 720 1187 1210 1545 1791 2803 2889
Size: 436 283 467 23 335 246 1012 86
Cuts at: 2889 2901 3247 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 436 346 335 283 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 310 433 557 631 658 310
Size: 123 124 74 27 3347
Fragments arranged by size:
3347 124 123 74 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 128 188 425 1280 1454 1845 1872 3217 128
Size: 60 237 855 174 391 27 1345 606
Fragments arranged by size:
1345 855 606 391 237 174 60 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 511 1718 1928 511
Size: 1207 210 2278
Fragments arranged by size:
2278 1207 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 573 834 573
Size: 261 3434
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 521 1129 1377 2399 3378 521
Size: 608 248 1022 979 838
Fragments arranged by size:
1022 979 838 608 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 1537 1567 1569 1599 140
Size: 32 1365 30 2 30 2236
Fragments arranged by size:
2236 1365 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 130 190 1456 130
Size: 60 1266 2369
Fragments arranged by size:
2369 1266 60
MslI CAynn'nnrTG
Cuts at: 160 376 526 532 1684 1811 160
Size: 216 150 6 1152 127 2044
Fragments arranged by size:
2044 1152 216 150 127 6
MspA1I CmG'CkG
Cuts at: 806 1120 1480 2493 2738 806
Size: 314 360 1013 245 1763
Fragments arranged by size:
1763 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 209 369 748 1248 2155 209
Size: 160 379 500 907 1749
Fragments arranged by size:
1749 907 500 379 160
PciI A'CATG_T
Cuts at: 205 2151 205
Size: 1946 1749
PfoI T'CCnGG_A
Cuts at: 199 199
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PpuMI rG'GwC_Cy
Cuts at: 573 573
Size: 3695
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 120 104
Size: 16 3679
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SexAI A'CCwGG_T
Cuts at: 341 341
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2416 2607 33
Size: 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StuI AGG'CCT
Cuts at: 466 466
Size: 3695
StyI C'CwwG_G
Cuts at: 829 1183 829
Size: 354 3341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 530 3210 530
Size: 2680 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI