Restriction map for
pF1KB7098
(Circular) (Six-base) MAPSORT of: pF1KB7098.seq Check: 7314 from: 1 to: 3707
pF1KB7098 3707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 277 277
Size: 3707
AatII G_ACGT'C
Cuts at: 659 659
Size: 3707
AccI GT'mk_AC
Cuts at: 613 745 613
Size: 132 3575
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 500 1059 2163 3140 500
Size: 559 1104 977 1067
Fragments arranged by size:
1104 1067 977 559
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AleI CACnn'nnGTG
Cuts at: 430 430
Size: 3707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 433 2477 433
Size: 2044 1663
ApoI r'AATT_y
Cuts at: 490 711 3060 3316 490
Size: 221 2349 256 881
Fragments arranged by size:
2349 881 256 221
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 242 723 1137 1385 1420 242
Size: 481 414 248 35 2529
Fragments arranged by size:
2529 481 414 248 35
BanII G_rGCy'C
Cuts at: 115 721 1751 115
Size: 606 1030 2071
Fragments arranged by size:
2071 1030 606
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 459 819 459
Size: 360 3347
Bme1580I G_kGCm'C
Cuts at: 437 1332 1425 2481 437
Size: 895 93 1056 1663
Fragments arranged by size:
1663 1056 895 93
BmrI ACTGGGnnnn_n'
Cuts at: 534 702 919 1000 1335 3479 534
Size: 168 217 81 335 2144 762
Fragments arranged by size:
2144 762 335 217 168 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 235 532 591 99
Size: 136 297 59 3215
Fragments arranged by size:
3215 297 136 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 656 1138 1386 2088 3584 656
Size: 482 248 702 1496 779
Fragments arranged by size:
1496 779 702 482 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 238 192
Size: 46 3661
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 115 437 721 1499 1689 2481 115
Size: 322 284 778 190 792 1341
Fragments arranged by size:
1341 792 778 322 284 190
Bsp1286I G_dGCh'C
Cuts at: 115 437 721 1332 1425 1499 1689 1751 2481
Size: 322 284 611 93 74 190 62 730
Cuts at: 2481 115
Size: 1341
Fragments arranged by size:
1341 730 611 322 284 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 277 757 971 1273 1654 277
Size: 480 214 302 381 2330
Fragments arranged by size:
2330 480 381 302 214
BsrBI CCG'CTC
Cuts at: 50 458 1999 2053 3537 3702 50
Size: 408 1541 54 1484 165 55
Fragments arranged by size:
1541 1484 408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 1619 185
Size: 1434 2273
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BssSI C'ACGA_G
Cuts at: 257 1978 2336 257
Size: 1721 358 1628
Fragments arranged by size:
1721 1628 358
BstAPI GCAn_nnn'nTGC
Cuts at: 269 1084 269
Size: 815 2892
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstXI CCAn_nnnn'nTGG
Cuts at: 259 604 259
Size: 345 3362
BstYI r'GATC_y
Cuts at: 1 293 395 732 1199 1222 1557 1803 2815
Size: 292 102 337 467 23 335 246 1012
Cuts at: 2815 2901 2913 3259 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 337 335 292 246
102 86 23 12
BtgI C'CryG_G
Cuts at: 383 383
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 231 950 231
Size: 719 2988
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DraIII CAC_nnn'GTG
Cuts at: 405 470 501 405
Size: 65 31 3611
Fragments arranged by size:
3611 65 31
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 719 113
Size: 606 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 235 532 591 1532 1964 2711 2979 99
Size: 136 297 59 941 432 747 268 827
Fragments arranged by size:
941 827 747 432 297 268 136 59
EcoNI CCTnn'n_nnAGG
Cuts at: 250 511 250
Size: 261 3446
EcoO109I rG'GnC_Cy
Cuts at: 846 846
Size: 3707
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 597 1141 1389 2411 3390 597
Size: 544 248 1022 979 914
Fragments arranged by size:
1022 979 914 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 533 565 655 687 1549 1579 1581 1611 533
Size: 32 90 32 862 30 2 30 2629
Fragments arranged by size:
2629 862 90 32 32 30 30 2
HincII GTy'rAC
Cuts at: 614 746 614
Size: 132 3575
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 430 1696 1823 430
Size: 1266 127 2314
Fragments arranged by size:
2314 1266 127
MspA1I CmG'CkG
Cuts at: 818 1132 1492 2505 2750 818
Size: 314 360 1013 245 1775
Fragments arranged by size:
1775 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 351 358 760 1260 2167 351
Size: 7 402 500 907 1891
Fragments arranged by size:
1891 907 500 402 7
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 447 754 1439 447
Size: 307 685 2715
Fragments arranged by size:
2715 685 307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 115 721 115
Size: 606 3101
SalI G'TCGA_C
Cuts at: 612 744 612
Size: 132 3575
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 443 750 1435 2428 2619 33
Size: 410 307 685 993 191 1121
Fragments arranged by size:
1121 993 685 410 307 191
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 358 760 1260 358
Size: 402 500 2805
Fragments arranged by size:
2805 500 402
StyI C'CwwG_G
Cuts at: 136 428 841 1195 136
Size: 292 413 354 2648
Fragments arranged by size:
2648 413 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 516 1290 1423 1882 2091 516
Size: 774 133 459 209 2132
Fragments arranged by size:
2132 774 459 209 133
TatI w'GTAC_w
Cuts at: 371 3222 371
Size: 2851 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
ZraI GAC'GTC
Cuts at: 657 657
Size: 3707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI