Restriction map for
pF1KB7105
(Circular) (Six-base) MAPSORT of: pF1KB7105.seq Check: 436 from: 1 to: 3785
pF1KB7105 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 489 489
Size: 3785
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1610 2042 2789 3057 1610
Size: 432 747 268 2338
Fragments arranged by size:
2338 747 432 268
AfeI AGC'GCT
Cuts at: 379 379
Size: 3785
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AleI CACnn'nnGTG
Cuts at: 256 322 445 256
Size: 66 123 3596
Fragments arranged by size:
3596 123 66
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 260 292 260
Size: 32 3753
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaI G_GGCC'C
Cuts at: 152 197 429 523 569 152
Size: 45 232 94 46 3368
Fragments arranged by size:
3368 232 94 46 45
ApaLI G'TGCA_C
Cuts at: 311 2555 311
Size: 2244 1541
ApoI r'AATT_y
Cuts at: 498 789 3138 3394 498
Size: 291 2349 256 889
Fragments arranged by size:
2349 889 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 371 464 622 805 371
Size: 93 158 183 3351
Fragments arranged by size:
3351 183 158 93
BamHI G'GATC_C
Cuts at: 693 810 693
Size: 117 3668
BanI G'GyrC_C
Cuts at: 329 364 801 1215 1463 1498 329
Size: 35 437 414 248 35 2616
Fragments arranged by size:
2616 437 414 248 35 35
BanII G_rGCy'C
Cuts at: 152 197 359 429 523 569 661 691 799
Size: 45 162 70 94 46 92 30 108
Cuts at: 799 1829 152
Size: 1030 2108
Fragments arranged by size:
2108 1030 162 108 94 92 70 46
45 30
BbeI G_GCGC'C
Cuts at: 333 1219 1467 333
Size: 886 248 2651
Fragments arranged by size:
2651 886 248
BbvCI CC'TCA_GC
Cuts at: 489 489
Size: 3785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3751
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglI GCCn_nnn'nGGC
Cuts at: 135 192 229 135
Size: 57 37 3691
Fragments arranged by size:
3691 57 37
BglII A'GATC_T
Cuts at: 734 1300 3337 734
Size: 566 2037 1182
Fragments arranged by size:
2037 1182 566
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 152 197 315 367 429 454 523 569 1410
Size: 45 118 52 62 25 69 46 841
Cuts at: 1410 1503 2559 152
Size: 93 1056 1378
Fragments arranged by size:
1378 1056 841 118 93 69 62 52
46 45 25
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 753 2152 147
Size: 606 1399 1780
Fragments arranged by size:
1780 1399 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 556 694 104
Size: 452 138 3195
Fragments arranged by size:
3195 452 138
Bpu10I CC'TnA_GC
Cuts at: 431 489 431
Size: 58 3727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 330 1216 1464 2166 3662 330
Size: 886 248 702 1496 453
Fragments arranged by size:
1496 886 702 453 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 353 353
Size: 3785
BseYI C'CCAG_C
Cuts at: 301 508 543 569 611 1210 1968 2545 3041
Size: 207 35 26 42 599 758 577 496
Cuts at: 3041 301
Size: 1045
Fragments arranged by size:
1045 758 599 577 496 207 42 35
26
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 288 315 556 799 1577 1767 2559 288
Size: 27 241 243 778 190 792 1514
Fragments arranged by size:
1514 792 778 243 241 190 27
Bsp1286I G_dGCh'C
Cuts at: 152 197 288 315 359 367 429 454 523
Size: 45 91 27 44 8 62 25 69
Cuts at: 523 556 569 661 691 799 1410 1503 1577
Size: 33 13 92 30 108 611 93 74
Cuts at: 1577 1767 1829 2559 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 190 108 93 92 91
74 69 62 62 45 44 33 30
27 25 13 8
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 835 1049 1351 1732 489
Size: 346 214 302 381 2542
Fragments arranged by size:
2542 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 1783 1964 2173 1783
Size: 181 209 3395
Fragments arranged by size:
3395 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstEII G'GTnAC_C
Cuts at: 720 720
Size: 3785
BstXI CCAn_nnnn'nTGG
Cuts at: 213 447 516 603 213
Size: 234 69 87 3395
Fragments arranged by size:
3395 234 87 69
BstYI r'GATC_y
Cuts at: 1 693 734 810 1277 1300 1635 1881 2893
Size: 692 41 76 467 23 335 246 1012
Cuts at: 2893 2979 2991 3337 1
Size: 86 12 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
76 41 23 12
BtgI C'CryG_G
Cuts at: 172 359 715 172
Size: 187 356 3242
Fragments arranged by size:
3242 356 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 181 1028 181
Size: 847 2938
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 221 679 1370 1544 1935 1962 3307 221
Size: 458 691 174 391 27 1345 699
Fragments arranged by size:
1345 699 691 458 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 244 1808 2018 244
Size: 1564 210 2011
Fragments arranged by size:
2011 1564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 268 280 1993 2303 2449 3595 268
Size: 12 1713 310 146 1146 458
Fragments arranged by size:
1713 1146 458 310 146 12
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 556 694 1610 2042 2789 3057 104
Size: 452 138 916 432 747 268 832
Fragments arranged by size:
916 832 747 452 432 268 138
EcoO109I rG'GnC_Cy
Cuts at: 148 149 194 426 520 540 565 566 587
Size: 1 45 232 94 20 25 1 21
Cuts at: 587 705 924 148
Size: 118 219 3009
Fragments arranged by size:
3009 232 219 118 94 45 25 21
20 1 1
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 333 381 1219 1467 2489 3468 333
Size: 48 838 248 1022 979 650
Fragments arranged by size:
1022 979 838 650 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 678 710 1627 1657 1659 1689 678
Size: 32 917 30 2 30 2774
Fragments arranged by size:
2774 917 32 30 30 2
HincII GTy'rAC
Cuts at: 116 824 116
Size: 708 3077
KasI G'GCGC_C
Cuts at: 329 1215 1463 329
Size: 886 248 2651
Fragments arranged by size:
2651 886 248
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 223 1546 223
Size: 1323 2462
MslI CAynn'nnrTG
Cuts at: 256 322 445 1774 1901 256
Size: 66 123 1329 127 2140
Fragments arranged by size:
2140 1329 127 123 66
MspA1I CmG'CkG
Cuts at: 256 678 896 1210 1570 2583 2828 256
Size: 422 218 314 360 1013 245 1213
Fragments arranged by size:
1213 1013 422 360 314 245 218
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 330 1216 1464 330
Size: 886 248 2651
Fragments arranged by size:
2651 886 248
NcoI C'CATG_G
Cuts at: 172 359 172
Size: 187 3598
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PasI CC'CwG_GG
Cuts at: 575 575
Size: 3785
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 540 540
Size: 3785
PspOMI G'GGCC_C
Cuts at: 148 193 425 519 565 148
Size: 45 232 94 46 3368
Fragments arranged by size:
3368 232 94 46 45
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 244 1808 2018 244
Size: 1564 210 2011
Fragments arranged by size:
2011 1564 210
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfoI GGC'GCC
Cuts at: 331 1217 1465 331
Size: 886 248 2651
Fragments arranged by size:
2651 886 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 940 1855 2347 2609 940
Size: 915 492 262 2116
Fragments arranged by size:
2116 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StuI AGG'CCT
Cuts at: 602 602
Size: 3785
StyI C'CwwG_G
Cuts at: 172 359 725 919 1273 172
Size: 187 366 194 354 2684
Fragments arranged by size:
2684 366 354 194 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 457 1368 1501 1960 2169 330
Size: 127 911 133 459 209 1946
Fragments arranged by size:
1946 911 459 209 133 127
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 685 717 3302 3334 685
Size: 32 2585 32 1136
Fragments arranged by size:
2585 1136 32 32
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 1280 258
Size: 1022 2763
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI