Restriction map for
pF1KB7106
(Circular) (Six-base) MAPSORT of: pF1KB7106.seq Check: 6833 from: 1 to: 3791
pF1KB7106 3791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AfeI AGC'GCT
Cuts at: 371 371
Size: 3791
AflIII A'CryG_T
Cuts at: 293 1143 2247 3224 293
Size: 850 1104 977 860
Fragments arranged by size:
1104 977 860 850
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 289 289
Size: 3791
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 3759
AlwNI CAG_nnn'CTG
Cuts at: 603 703 1096 2663 603
Size: 100 393 1567 1731
Fragments arranged by size:
1731 1567 393 100
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3791
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 448 707 795 3144 3400 448
Size: 259 88 2349 256 839
Fragments arranged by size:
2349 839 259 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 807 1221 1469 1504 807
Size: 414 248 35 3094
Fragments arranged by size:
3094 414 248 35
BanII G_rGCy'C
Cuts at: 342 805 1835 342
Size: 463 1030 2298
Fragments arranged by size:
2298 1030 463
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 558 669 676 558
Size: 111 7 3673
Fragments arranged by size:
3673 111 7
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 173 1306 3343 173
Size: 1133 2037 621
Fragments arranged by size:
2037 1133 621
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 121 342 1416 1509 2565 121
Size: 221 1074 93 1056 1347
Fragments arranged by size:
1347 1074 1056 221 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 390 1144 390
Size: 754 3037
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 398 980 1501 2179 2453 2600 3011 398
Size: 582 521 678 274 147 411 1178
Fragments arranged by size:
1178 678 582 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 574 604 139
Size: 30 405 30 3326
Fragments arranged by size:
3326 405 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 478
Size: 3791
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 121 342 805 1416 1509 1583 1773 1835 2565
Size: 221 463 611 93 74 190 62 730
Cuts at: 2565 121
Size: 1347
Fragments arranged by size:
1347 730 611 463 221 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 689 2967 689
Size: 2278 1513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 545 2152 545
Size: 1607 2184
BstXI CCAn_nnnn'nTGG
Cuts at: 390 639 390
Size: 249 3542
BstYI r'GATC_y
Cuts at: 1 173 816 1283 1306 1641 1887 2899 2985
Size: 172 643 467 23 335 246 1012 86
Cuts at: 2985 2997 3343 1
Size: 12 346 449
Fragments arranged by size:
1012 643 467 449 346 335 246 172
86 23 12
BtgI C'CryG_G
Cuts at: 107 662 107
Size: 555 3236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 704 1034 704
Size: 330 3461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 147 182 147
Size: 35 3756
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 380 1376 1550 1941 1968 3313 380
Size: 996 174 391 27 1345 858
Fragments arranged by size:
1345 996 858 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 196 409 1814 2024 196
Size: 213 1405 210 1963
Fragments arranged by size:
1963 1405 213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 339 930 339
Size: 591 3200
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 693 1810 693
Size: 1117 2674
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 373 1225 1473 2495 3474 373
Size: 852 248 1022 979 690
Fragments arranged by size:
1022 979 852 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1633 1663 1665 1695 574
Size: 32 1027 30 2 30 2670
Fragments arranged by size:
2670 1027 32 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 382 1552 382
Size: 1170 2621
MslI CAynn'nnrTG
Cuts at: 388 1780 1907 388
Size: 1392 127 2272
Fragments arranged by size:
2272 1392 127
MspA1I CmG'CkG
Cuts at: 348 600 902 1216 1576 2589 2834 348
Size: 252 302 314 360 1013 245 1305
Fragments arranged by size:
1305 1013 360 314 302 252 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PmlI CAC'GTG
Cuts at: 390 390
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 139 171 2930 2962 139
Size: 32 2759 32 968
Fragments arranged by size:
2759 968 32 32
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3791
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 283 315 283
Size: 32 3759
PstI C_TGCA'G
Cuts at: 128 278 347 838 1523 128
Size: 150 69 491 685 2396
Fragments arranged by size:
2396 685 491 150 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 348 1216 1576 348
Size: 868 360 2563
Fragments arranged by size:
2563 868 360
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 124 274 343 834 1519 2512 2703 33
Size: 91 150 69 491 685 993 191 1121
Fragments arranged by size:
1121 993 685 491 191 150 91 69
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 107 925 1279 107
Size: 818 354 2619
Fragments arranged by size:
2619 818 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 259 1588 259
Size: 1329 2462
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 1286 600
Size: 686 3105
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI