Restriction map for
pF1KB7107
(Circular) (Six-base) MAPSORT of: pF1KB7107.seq Check: 6513 from: 1 to: 3800
pF1KB7107 3800 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 301 529 757 838 301
Size: 228 228 81 3263
Fragments arranged by size:
3263 228 228 81
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1625 2057 2804 3072 1625
Size: 432 747 268 2353
Fragments arranged by size:
2353 747 432 268
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 1105 2672 1105
Size: 1567 2233
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 820 820
Size: 3800
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 816 1230 1478 1513 816
Size: 414 248 35 3103
Fragments arranged by size:
3103 414 248 35
BanII G_rGCy'C
Cuts at: 164 392 620 814 1844 164
Size: 228 228 194 1030 2120
Fragments arranged by size:
2120 1030 228 228 194
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BbsI GAAGACnn'nnnn_
Cuts at: 358 586 358
Size: 228 3572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3766
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglII A'GATC_T
Cuts at: 113 341 569 1315 3352 113
Size: 228 228 746 2037 561
Fragments arranged by size:
2037 746 561 228 228
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 1425 1518 2574 1425
Size: 93 1056 2651
Fragments arranged by size:
2651 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 386 614 1012 1093 1428 3572 158
Size: 228 228 398 81 335 2144 386
Fragments arranged by size:
2144 398 386 335 228 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 3800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 976 1855 2347 2645 172
Size: 804 879 492 298 1327
Fragments arranged by size:
1327 879 804 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 1153 1153
Size: 3800
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 1231 1479 2181 3677 1231
Size: 248 702 1496 1354
Fragments arranged by size:
1496 1354 702 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1225 1983 2560 3056 1225
Size: 758 577 496 1969
Fragments arranged by size:
1969 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 520 748 292
Size: 228 228 3344
Fragments arranged by size:
3344 228 228
BsiEI CG_ry'CG
Cuts at: 104 1342 1388 2596 104
Size: 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 46
BsiHKAI G_wGCw'C
Cuts at: 814 1592 1782 2574 814
Size: 778 190 792 2040
Fragments arranged by size:
2040 792 778 190
BsmI GAATG_Cn'
Cuts at: 212 212
Size: 3800
Bsp1286I G_dGCh'C
Cuts at: 164 392 620 814 1425 1518 1592 1782 1844
Size: 228 228 194 611 93 74 190 62
Cuts at: 1844 2574 164
Size: 730 1390
Fragments arranged by size:
1390 730 611 228 228 194 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1064 1366 1747 850
Size: 214 302 381 2903
Fragments arranged by size:
2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2092 2146 3630 3795 50
Size: 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1712 1712
Size: 3800
BsrFI r'CCGG_y
Cuts at: 133 361 589 1798 1979 2188 133
Size: 228 228 1209 181 209 1745
Fragments arranged by size:
1745 1209 228 228 209 181
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstXI CCAn_nnnn'nTGG
Cuts at: 378 606 378
Size: 228 3572
BstYI r'GATC_y
Cuts at: 1 113 194 341 422 569 650 825 1292
Size: 112 81 147 81 147 81 175 467
Cuts at: 1292 1315 1650 1896 2908 2994 3006 3352 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 175 147
147 112 86 81 81 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 781 781
Size: 3800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1043
Size: 3800
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 264 492 720 1385 1559 1950 1977 3322 264
Size: 228 228 665 174 391 27 1345 742
Fragments arranged by size:
1345 742 665 391 228 228 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1625 2057 2804 3072 628
Size: 997 432 747 268 1356
Fragments arranged by size:
1356 997 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 939 939
Size: 3800
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 1234 1482 2504 3483 1234
Size: 248 1022 979 1551
Fragments arranged by size:
1551 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 1642 1672 1674 1704 588
Size: 32 1022 30 2 30 2684
Fragments arranged by size:
2684 1022 32 30 30 2
HincII GTy'rAC
Cuts at: 302 530 758 839 302
Size: 228 228 81 3263
Fragments arranged by size:
3263 228 228 81
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2655 2471
Size: 184 3616
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 1789 1916 1789
Size: 127 3673
MspA1I CmG'CkG
Cuts at: 255 483 711 911 1225 1585 2598 2843 255
Size: 228 228 200 314 360 1013 245 1212
Fragments arranged by size:
1212 1013 360 314 245 228 228 200
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 853 1353 2260 853
Size: 500 907 2393
Fragments arranged by size:
2393 907 500
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PfoI T'CCnGG_A
Cuts at: 197 425 197
Size: 228 3572
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 255 483 711 1225 1585 255
Size: 228 228 514 360 2470
Fragments arranged by size:
2470 514 360 228 228
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 300 528 756 837 300
Size: 228 228 81 3263
Fragments arranged by size:
3263 228 228 81
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SexAI A'CCwGG_T
Cuts at: 311 539 767 311
Size: 228 228 3344
Fragments arranged by size:
3344 228 228
SfcI C'TryA_G
Cuts at: 33 843 1528 2521 2712 33
Size: 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 151 955 1870 2362 2624 151
Size: 804 915 492 262 1327
Fragments arranged by size:
1327 915 804 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
StyI C'CwwG_G
Cuts at: 934 1288 934
Size: 354 3446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 3315 3315
Size: 3800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1295
Size: 3800
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
XmnI GAAnn'nnTTC
Cuts at: 212 212
Size: 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI