Restriction map for
pF1KB7109
(Circular) (Six-base) MAPSORT of: pF1KB7109.seq Check: 6275 from: 1 to: 3857
pF1KB7109 3857 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AflII C'TTAA_G
Cuts at: 393 393
Size: 3857
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 1162 2729 1162
Size: 1567 2290
ApaI G_GGCC'C
Cuts at: 662 662
Size: 3857
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 601 861 3210 3466 601
Size: 260 2349 256 992
Fragments arranged by size:
2349 992 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 873 1287 1535 1570 873
Size: 414 248 35 3160
Fragments arranged by size:
3160 414 248 35
BanII G_rGCy'C
Cuts at: 662 871 1901 662
Size: 209 1030 2618
Fragments arranged by size:
2618 1030 209
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbsI GAAGACnn'nnnn_
Cuts at: 529 529
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 662 1482 1575 2631 662
Size: 820 93 1056 1888
Fragments arranged by size:
1888 1056 820 93
BmrI ACTGGGnnnn_n'
Cuts at: 524 1069 1150 1485 3629 524
Size: 545 81 335 2144 752
Fragments arranged by size:
2144 752 545 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 212 242 212
Size: 30 3827
BseYI C'CCAG_C
Cuts at: 688 745 1282 2040 2617 3113 688
Size: 57 537 758 577 496 1432
Fragments arranged by size:
1432 758 577 537 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 264
Size: 3857
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 662 871 1482 1575 1649 1839 1901 2631 662
Size: 209 611 93 74 190 62 730 1888
Fragments arranged by size:
1888 730 611 209 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 743 907 1121 1423 1804 743
Size: 164 214 302 381 2796
Fragments arranged by size:
2796 381 302 214 164
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 627 1769 627
Size: 1142 2715
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 442 1234 442
Size: 792 3065
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 882 1349 1372 1707 1953 2965 3051 3063
Size: 881 467 23 335 246 1012 86 12
Cuts at: 3063 3409 1
Size: 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3857
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 449 852 1100 449
Size: 403 248 3206
Fragments arranged by size:
3206 403 248
DraI TTT'AAA
Cuts at: 812 856 812
Size: 44 3813
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 755 1442 1616 2007 2034 3379 755
Size: 687 174 391 27 1345 1233
Fragments arranged by size:
1345 1233 687 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 659 996 659
Size: 337 3520
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 1291 1539 2561 3540 1291
Size: 248 1022 979 1608
Fragments arranged by size:
1608 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 395 427 493 525 1699 1729 1731 1761 395
Size: 32 66 32 1174 30 2 30 2491
Fragments arranged by size:
2491 1174 66 32 32 30 30 2
HincII GTy'rAC
Cuts at: 187 896 187
Size: 709 3148
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2712 2528
Size: 184 3673
MscI TGG'CCA
Cuts at: 757 1618 757
Size: 861 2996
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 181 968 1282 1642 2655 2900 181
Size: 787 314 360 1013 245 1138
Fragments arranged by size:
1138 1013 787 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NcoI C'CATG_G
Cuts at: 107 107
Size: 3857
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 544 764 910 1410 2317 544
Size: 220 146 500 907 2084
Fragments arranged by size:
2084 907 500 220 146
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 658 658
Size: 3857
PstI C_TGCA'G
Cuts at: 904 1589 904
Size: 685 3172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 181 1282 1642 181
Size: 1101 360 2396
Fragments arranged by size:
2396 1101 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 900 1585 2578 2769 33
Size: 867 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 191
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 393 1012 1927 2419 2681 393
Size: 619 915 492 262 1569
Fragments arranged by size:
1569 915 619 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
SspI AAT'ATT
Cuts at: 306 306
Size: 3857
StyI C'CwwG_G
Cuts at: 107 991 1345 107
Size: 884 354 2619
Fragments arranged by size:
2619 884 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 543 1440 1573 2032 2241 543
Size: 897 133 459 209 2159
Fragments arranged by size:
2159 897 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 3825
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI