Restriction map for
pF1KB7110
(Circular) (Six-base) MAPSORT of: pF1KB7110.seq Check: 7292 from: 1 to: 3893
pF1KB7110 3893 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 931 931
Size: 3893
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 1718 2150 2897 3165 537
Size: 1181 432 747 268 1265
Fragments arranged by size:
1265 1181 747 432 268
AflIII A'CryG_T
Cuts at: 1245 2349 3326 1245
Size: 1104 977 1812
Fragments arranged by size:
1812 1104 977
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AlwNI CAG_nnn'CTG
Cuts at: 381 1198 2765 381
Size: 817 1567 1509
Fragments arranged by size:
1567 1509 817
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 897 3246 3502 897
Size: 2349 256 1288
Fragments arranged by size:
2349 1288 256
AseI AT'TA_AT
Cuts at: 20 874 20
Size: 854 3039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 913 913
Size: 3893
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 196 495 738 909 1323 1571 1606 196
Size: 299 243 171 414 248 35 2483
Fragments arranged by size:
2483 414 299 248 243 171 35
BanII G_rGCy'C
Cuts at: 416 907 1937 416
Size: 491 1030 2372
Fragments arranged by size:
2372 1030 491
BbeI G_GCGC'C
Cuts at: 200 1327 1575 200
Size: 1127 248 2518
Fragments arranged by size:
2518 1127 248
BbsI GAAGACnn'nnnn_
Cuts at: 528 765 528
Size: 237 3656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 415
Size: 34 3859
BciVI GTATCCnnnnn_n'
Cuts at: 1791 2552 3893 1791
Size: 761 1341 1791
Fragments arranged by size:
1791 1341 761
BclI T'GATC_A
Cuts at: 1413 1413
Size: 3893
BglII A'GATC_T
Cuts at: 503 1408 3445 503
Size: 905 2037 951
Fragments arranged by size:
2037 951 905
BlpI GC'TnA_GC
Cuts at: 1005 1005
Size: 3893
Bme1580I G_kGCm'C
Cuts at: 743 1518 1611 2667 743
Size: 775 93 1056 1969
Fragments arranged by size:
1969 1056 775 93
BmrI ACTGGGnnnn_n'
Cuts at: 678 1105 1186 1521 3665 678
Size: 427 81 335 2144 906
Fragments arranged by size:
2144 906 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 706 1069 1948 2440 2738 559
Size: 147 363 879 492 298 1714
Fragments arranged by size:
1714 879 492 363 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 1246 1246
Size: 3893
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 197 1324 1572 2274 3770 197
Size: 1127 248 702 1496 320
Fragments arranged by size:
1496 1127 702 320 248
BsaWI w'CCGG_w
Cuts at: 1082 1603 2281 2555 2702 3113 1082
Size: 521 678 274 147 411 1862
Fragments arranged by size:
1862 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 3863
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 507 612 712 715 507
Size: 105 100 3 3685
Fragments arranged by size:
3685 105 100 3
BseYI C'CCAG_C
Cuts at: 1318 2076 2653 3149 1318
Size: 758 577 496 2062
Fragments arranged by size:
2062 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 487 394
Size: 93 3800
BsiEI CG_ry'CG
Cuts at: 104 1435 1481 2689 104
Size: 1331 46 1208 1308
Fragments arranged by size:
1331 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 907 1685 1875 2667 416
Size: 491 778 190 792 1642
Fragments arranged by size:
1642 792 778 491 190
BsmI GAATG_Cn'
Cuts at: 438 438
Size: 3893
Bsp1286I G_dGCh'C
Cuts at: 416 743 907 1518 1611 1685 1875 1937 2667
Size: 327 164 611 93 74 190 62 730
Cuts at: 2667 416
Size: 1642
Fragments arranged by size:
1642 730 611 327 190 164 93 74
62
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 3069 3069
Size: 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 943 1157 1459 1840 323
Size: 620 214 302 381 2376
Fragments arranged by size:
2376 620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2185 2239 3723 3888 50
Size: 2135 54 1484 165 55
Fragments arranged by size:
2135 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1805 1805
Size: 3893
BsrFI r'CCGG_y
Cuts at: 1891 2072 2281 1891
Size: 181 209 3503
Fragments arranged by size:
3503 209 181
BssSI C'ACGA_G
Cuts at: 2164 2522 2164
Size: 358 3535
BstAPI GCAn_nnn'nTGC
Cuts at: 563 1270 563
Size: 707 3186
BstBI TT'CG_AA
Cuts at: 2254 2254
Size: 3893
BstYI r'GATC_y
Cuts at: 1 503 918 1385 1408 1743 1989 3001 3087
Size: 502 415 467 23 335 246 1012 86
Cuts at: 3087 3099 3445 1
Size: 12 346 449
Fragments arranged by size:
1012 502 467 449 415 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 320 320
Size: 3893
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 821 1843 2024 2187 821
Size: 1022 181 163 2527
Fragments arranged by size:
2527 1022 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1136
Size: 3893
ClaI AT'CG_AT
Cuts at: 821 821
Size: 3893
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 674 1478 1652 2043 2070 3415 674
Size: 804 174 391 27 1345 1152
Fragments arranged by size:
1345 1152 804 391 174 27
EagI C'GGCC_G
Cuts at: 1478 1478
Size: 3893
EarI CTCTTCn'nnn_
Cuts at: 525 574 601 776 1916 2126 525
Size: 49 27 175 1140 210 2292
Fragments arranged by size:
2292 1140 210 175 49 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 2411 2557 3703 2101
Size: 310 146 1146 2291
Fragments arranged by size:
2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 414 905 414
Size: 491 3402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 1718 2150 2897 3165 537
Size: 1181 432 747 268 1265
Fragments arranged by size:
1265 1181 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 319 385 694 319
Size: 66 309 3518
Fragments arranged by size:
3518 309 66
EcoO109I rG'GnC_Cy
Cuts at: 390 645 687 1032 390
Size: 255 42 345 3251
Fragments arranged by size:
3251 345 255 42
EcoRI G'AATT_C
Cuts at: 897 897
Size: 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1912 1912
Size: 3893
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 200 1327 1575 2597 3576 200
Size: 1127 248 1022 979 517
Fragments arranged by size:
1127 1022 979 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1735 1765 1767 1797 1735
Size: 30 2 30 3831
Fragments arranged by size:
3831 30 30 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 196 1323 1571 196
Size: 1127 248 2518
Fragments arranged by size:
2518 1127 248
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 2564 2748 137
Size: 2427 184 1282
Fragments arranged by size:
2427 1282 184
MscI TGG'CCA
Cuts at: 676 1654 676
Size: 978 2915
MslI CAynn'nnrTG
Cuts at: 836 1882 2009 836
Size: 1046 127 2720
Fragments arranged by size:
2720 1046 127
MspA1I CmG'CkG
Cuts at: 209 1004 1318 1678 2691 2936 209
Size: 795 314 360 1013 245 1166
Fragments arranged by size:
1166 1013 795 360 314 245
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 3893
NarI GG'CG_CC
Cuts at: 197 1324 1572 197
Size: 1127 248 2518
Fragments arranged by size:
2518 1127 248
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 3893
NspI r_CATG'y
Cuts at: 841 946 1446 2353 841
Size: 105 500 907 2381
Fragments arranged by size:
2381 907 500 105
PciI A'CATG_T
Cuts at: 2349 2349
Size: 3893
PflMI CCAn_nnn'nTGG
Cuts at: 336 336
Size: 3893
PfoI T'CCnGG_A
Cuts at: 329 329
Size: 3893
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3861
PpuMI rG'GwC_Cy
Cuts at: 645 645
Size: 3893
PstI C_TGCA'G
Cuts at: 398 940 1625 398
Size: 542 685 2666
Fragments arranged by size:
2666 685 542
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 1318 1678 1318
Size: 360 3533
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 416 907 416
Size: 491 3402
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 601 1916 2126 601
Size: 1315 210 2368
Fragments arranged by size:
2368 1315 210
SbfI CC_TGCA'GG
Cuts at: 398 940 398
Size: 542 3351
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SexAI A'CCwGG_T
Cuts at: 368 368
Size: 3893
SfcI C'TryA_G
Cuts at: 33 394 507 936 1621 2614 2805 33
Size: 361 113 429 685 993 191 1121
Fragments arranged by size:
1121 993 685 429 361 191 113
SfoI GGC'GCC
Cuts at: 198 1325 1573 198
Size: 1127 248 2518
Fragments arranged by size:
2518 1127 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 915 915
Size: 3893
SmlI C'TyrA_G
Cuts at: 574 721 1048 1963 2455 2717 574
Size: 147 327 915 492 262 1750
Fragments arranged by size:
1750 915 492 327 262 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
StyI C'CwwG_G
Cuts at: 419 582 1027 1381 419
Size: 163 445 354 2931
Fragments arranged by size:
2931 445 354 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 660 723 848 1476 1609 2068 2277 660
Size: 63 125 628 133 459 209 2276
Fragments arranged by size:
2276 628 459 209 133 125 63
TatI w'GTAC_w
Cuts at: 704 3408 704
Size: 2704 1189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 3861
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 924 924
Size: 3893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1388 1388
Size: 3893
XmaI C'CCGG_G
Cuts at: 913 913
Size: 3893
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI