Restriction map for
pF1KB7111
(Circular) (Six-base) MAPSORT of: pF1KB7111.seq Check: 3004 from: 1 to: 3896
pF1KB7111 3896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 602 602
Size: 3896
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 587 912 587
Size: 325 3571
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AfeI AGC'GCT
Cuts at: 371 371
Size: 3896
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 289 753 289
Size: 464 3432
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3896
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 587 912 1326 1574 1609 587
Size: 325 414 248 35 2874
Fragments arranged by size:
2874 414 325 248 35
BanII G_rGCy'C
Cuts at: 342 910 1940 342
Size: 568 1030 2298
Fragments arranged by size:
2298 1030 568
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbsI GAAGACnn'nnnn_
Cuts at: 676 676
Size: 3896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 466 500 466
Size: 34 3862
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BfrBI ATG'CAT
Cuts at: 387 387
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 342 1521 1614 2670 342
Size: 1179 93 1056 1568
Fragments arranged by size:
1568 1179 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 328
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 139
Size: 30 3866
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 446 446
Size: 3896
BseYI C'CCAG_C
Cuts at: 366 1321 2079 2656 3152 366
Size: 955 758 577 496 1110
Fragments arranged by size:
1110 955 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 109
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 783 910 1688 1878 2670 783
Size: 127 778 190 792 2009
Fragments arranged by size:
2009 792 778 190 127
BsmBI CGTCTCn'nnnn_
Cuts at: 363 363
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 342 783 910 1521 1614 1688 1878 1940 2670
Size: 441 127 611 93 74 190 62 730
Cuts at: 2670 342
Size: 1568
Fragments arranged by size:
1568 730 611 441 190 127 93 74
62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 206 3072 206
Size: 2866 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 752 946 1160 1462 1843 602
Size: 150 194 214 302 381 2655
Fragments arranged by size:
2655 381 302 214 194 150
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 214 2167 2525 214
Size: 1953 358 1585
Fragments arranged by size:
1953 1585 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 390 390
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 271 1139 271
Size: 868 3028
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 380 1481 1655 2046 2073 3418 380
Size: 1101 174 391 27 1345 858
Fragments arranged by size:
1345 1101 858 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 433 1919 2129 433
Size: 1486 210 2200
Fragments arranged by size:
2200 1486 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1721 2153 2900 3168 328
Size: 1393 432 747 268 1056
Fragments arranged by size:
1393 1056 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 399 399
Size: 3896
EcoO109I rG'GnC_Cy
Cuts at: 339 1035 339
Size: 696 3200
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 373 1330 1578 2600 3579 373
Size: 957 248 1022 979 690
Fragments arranged by size:
1022 979 957 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 1738 1768 1770 1800 194
Size: 32 1512 30 2 30 2290
Fragments arranged by size:
2290 1512 32 30 30 2
HincII GTy'rAC
Cuts at: 147 642 935 147
Size: 495 293 3108
Fragments arranged by size:
3108 495 293
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 591 916 591
Size: 325 3571
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 382 1657 382
Size: 1275 2621
MslI CAynn'nnrTG
Cuts at: 388 688 1885 2012 388
Size: 300 1197 127 2272
Fragments arranged by size:
2272 1197 300 127
MspA1I CmG'CkG
Cuts at: 348 1007 1321 1681 2694 2939 348
Size: 659 314 360 1013 245 1305
Fragments arranged by size:
1305 1013 659 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NsiI A_TGCA'T
Cuts at: 389 389
Size: 3896
NspI r_CATG'y
Cuts at: 391 717 949 1449 2356 391
Size: 326 232 500 907 1931
Fragments arranged by size:
1931 907 500 326 232
PasI CC'CwG_GG
Cuts at: 599 599
Size: 3896
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3896
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 348 1321 1681 348
Size: 973 360 2563
Fragments arranged by size:
2563 973 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 399 939 1624 2617 2808 33
Size: 366 540 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 366 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 717 949 1449 717
Size: 232 500 3164
Fragments arranged by size:
3164 500 232
StyI C'CwwG_G
Cuts at: 107 1030 1384 107
Size: 923 354 2619
Fragments arranged by size:
2619 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 591 1479 1612 2071 2280 591
Size: 888 133 459 209 2207
Fragments arranged by size:
2207 888 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 259 1693 259
Size: 1434 2462
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 448 448
Size: 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI