Restriction map for
pF1KB7114
(Circular) (Six-base) MAPSORT of: pF1KB7114.seq Check: 9181 from: 1 to: 3932
pF1KB7114 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 805 948 805
Size: 143 3789
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 2189 2936 3204 1757
Size: 432 747 268 2485
Fragments arranged by size:
2485 747 432 268
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3932
ApaLI G'TGCA_C
Cuts at: 783 2702 783
Size: 1919 2013
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 899 952 899
Size: 53 3879
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 102 135 102
Size: 33 3899
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 419 449 805 948 1362 1610 1645 419
Size: 30 356 143 414 248 35 2706
Fragments arranged by size:
2706 414 356 248 143 35 30
BanII G_rGCy'C
Cuts at: 342 488 722 865 946 1976 342
Size: 146 234 143 81 1030 2298
Fragments arranged by size:
2298 1030 234 146 143 81
BbeI G_GCGC'C
Cuts at: 453 1366 1614 453
Size: 913 248 2771
Fragments arranged by size:
2771 913 248
BbsI GAAGACnn'nnnn_
Cuts at: 183 183
Size: 3932
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglI GCCn_nnn'nGGC
Cuts at: 281 281
Size: 3932
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 342 787 1557 1650 2706 342
Size: 445 770 93 1056 1568
Fragments arranged by size:
1568 1056 770 445 93
BmrI ACTGGGnnnn_n'
Cuts at: 1144 1225 1560 3704 1144
Size: 81 335 2144 1372
Fragments arranged by size:
2144 1372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 135
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1108 1987 2479 2777 758
Size: 350 879 492 298 1913
Fragments arranged by size:
1913 879 492 350 298
BsaI GGTCTCn'nnnn_
Cuts at: 896 3636 896
Size: 2740 1192
BsaAI yAC'GTr
Cuts at: 390 1285 390
Size: 895 3037
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 450 1363 1611 2313 3809 450
Size: 913 248 702 1496 573
Fragments arranged by size:
1496 913 702 573 248
BsaWI w'CCGG_w
Cuts at: 1121 1642 2320 2594 2741 3152 1121
Size: 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 139
Size: 30 3902
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 869 869
Size: 3932
BseYI C'CCAG_C
Cuts at: 1357 2115 2692 3188 1357
Size: 758 577 496 2101
Fragments arranged by size:
2101 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 227 1474 1520 2728 104
Size: 123 1247 46 1208 1308
Fragments arranged by size:
1308 1247 1208 123 46
BsiHKAI G_wGCw'C
Cuts at: 722 787 946 1724 1914 2706 722
Size: 65 159 778 190 792 1948
Fragments arranged by size:
1948 792 778 190 159 65
Bsp1286I G_dGCh'C
Cuts at: 342 488 722 787 865 946 1557 1650 1724
Size: 146 234 65 78 81 611 93 74
Cuts at: 1724 1914 1976 2706 342
Size: 190 62 730 1568
Fragments arranged by size:
1568 730 611 234 190 146 93 81
78 74 65 62
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 982 1196 1498 1879 982
Size: 214 302 381 3035
Fragments arranged by size:
3035 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 1930 2111 2320 1930
Size: 181 209 3542
Fragments arranged by size:
3542 209 181
BssSI C'ACGA_G
Cuts at: 376 712 748 2203 2561 376
Size: 336 36 1455 358 1747
Fragments arranged by size:
1747 1455 358 336 36
BstAPI GCAn_nnn'nTGC
Cuts at: 743 1309 743
Size: 566 3366
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstEII G'GTnAC_C
Cuts at: 844 844
Size: 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 624 624
Size: 3932
BstYI r'GATC_y
Cuts at: 1 606 957 1424 1447 1782 2028 3040 3126
Size: 605 351 467 23 335 246 1012 86
Cuts at: 3126 3138 3484 1
Size: 12 346 449
Fragments arranged by size:
1012 605 467 449 351 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 432 801 432
Size: 369 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 519 1882 2063 2226 519
Size: 1363 181 163 2225
Fragments arranged by size:
2225 1363 181 163
BtsI GCAGTG_nn'
Cuts at: 370 1175 370
Size: 805 3127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 147 182 147
Size: 35 3897
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 224 680 1517 1691 2082 2109 3454 224
Size: 456 837 174 391 27 1345 702
Fragments arranged by size:
1345 837 702 456 391 174 27
EagI C'GGCC_G
Cuts at: 224 1517 224
Size: 1293 2639
EarI CTCTTCn'nnn_
Cuts at: 409 676 819 1955 2165 409
Size: 267 143 1136 210 2176
Fragments arranged by size:
2176 1136 267 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2450 2596 3742 2140
Size: 310 146 1146 2330
Fragments arranged by size:
2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 720 944 720
Size: 224 3708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 1757 2189 2936 3204 135
Size: 1622 432 747 268 863
Fragments arranged by size:
1622 863 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 339 1071 339
Size: 732 3200
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1951 1951
Size: 3932
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 453 1366 1614 2636 3615 453
Size: 913 248 1022 979 770
Fragments arranged by size:
1022 979 913 770 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1774 1804 1806 1836 1774
Size: 30 2 30 3870
Fragments arranged by size:
3870 30 30 2
HincII GTy'rAC
Cuts at: 147 971 147
Size: 824 3108
KasI G'GCGC_C
Cuts at: 449 1362 1610 449
Size: 913 248 2771
Fragments arranged by size:
2771 913 248
KpnI G_GTAC'C
Cuts at: 809 952 809
Size: 143 3789
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 2603 2787 314
Size: 2289 184 1459
Fragments arranged by size:
2289 1459 184
MscI TGG'CCA
Cuts at: 682 1693 682
Size: 1011 2921
MslI CAynn'nnrTG
Cuts at: 388 1921 2048 388
Size: 1533 127 2272
Fragments arranged by size:
2272 1533 127
MspA1I CmG'CkG
Cuts at: 688 1043 1357 1717 2730 2975 688
Size: 355 314 360 1013 245 1645
Fragments arranged by size:
1645 1013 360 355 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 450 1363 1611 450
Size: 913 248 2771
Fragments arranged by size:
2771 913 248
NcoI C'CATG_G
Cuts at: 432 432
Size: 3932
NdeI CA'TA_TG
Cuts at: 498 498
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 365 985 1485 2392 365
Size: 620 500 907 1905
Fragments arranged by size:
1905 907 620 500
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PmlI CAC'GTG
Cuts at: 390 390
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PshAI GACnn'nnGTC
Cuts at: 499 499
Size: 3932
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3932
PstI C_TGCA'G
Cuts at: 347 508 979 1664 347
Size: 161 471 685 2615
Fragments arranged by size:
2615 685 471 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 688 1357 1717 688
Size: 669 360 2903
Fragments arranged by size:
2903 669 360
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 722 946 722
Size: 224 3708
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 154 3449 154
Size: 3295 637
SexAI A'CCwGG_T
Cuts at: 215 728 215
Size: 513 3419
SfcI C'TryA_G
Cuts at: 33 343 504 975 1660 2653 2844 33
Size: 310 161 471 685 993 191 1121
Fragments arranged by size:
1121 993 685 471 310 191 161
SfoI GGC'GCC
Cuts at: 451 1364 1612 451
Size: 913 248 2771
Fragments arranged by size:
2771 913 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 954 954
Size: 3932
SmlI C'TyrA_G
Cuts at: 773 1087 2002 2494 2756 773
Size: 314 915 492 262 1949
Fragments arranged by size:
1949 915 492 314 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StyI C'CwwG_G
Cuts at: 432 1066 1420 432
Size: 634 354 2944
Fragments arranged by size:
2944 634 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1515 1648 2107 2316 1515
Size: 133 459 209 3131
Fragments arranged by size:
3131 459 209 133
TatI w'GTAC_w
Cuts at: 152 3447 152
Size: 3295 637
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1427 1427
Size: 3932
XmaI C'CCGG_G
Cuts at: 952 952
Size: 3932
XmnI GAAnn'nnTTC
Cuts at: 661 661
Size: 3932
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PfoI
PpuMI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI