Restriction map for
pF1KB7118
(Circular) (Six-base) MAPSORT of: pF1KB7118.seq Check: 5828 from: 1 to: 3962
pF1KB7118 3962 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 540 1787 2219 2966 3234 217
Size: 323 1247 432 747 268 945
Fragments arranged by size:
1247 945 747 432 323 268
AflIII A'CryG_T
Cuts at: 1314 2418 3395 1314
Size: 1104 977 1881
Fragments arranged by size:
1881 1104 977
AgeI A'CCGG_T
Cuts at: 2350 2350
Size: 3962
AhdI GACnn_n'nnGTC
Cuts at: 775 775
Size: 3962
AleI CACnn'nnGTG
Cuts at: 950 950
Size: 3962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 643 675 643
Size: 32 3930
AlwNI CAG_nnn'CTG
Cuts at: 1267 2834 1267
Size: 1567 2395
ApaI G_GGCC'C
Cuts at: 314 337 547 314
Size: 23 210 3729
Fragments arranged by size:
3729 210 23
ApaLI G'TGCA_C
Cuts at: 2732 2732
Size: 3962
ApoI r'AATT_y
Cuts at: 966 3315 3571 966
Size: 2349 256 1357
Fragments arranged by size:
2349 1357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 261 548 592 982 261
Size: 287 44 390 3241
Fragments arranged by size:
3241 390 287 44
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 978 1392 1640 1675 978
Size: 414 248 35 3265
Fragments arranged by size:
3265 414 248 35
BanII G_rGCy'C
Cuts at: 247 314 337 415 547 579 976 2006 247
Size: 67 23 78 132 32 397 1030 2203
Fragments arranged by size:
2203 1030 397 132 78 67 32 23
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BbvCI CC'TCA_GC
Cuts at: 292 480 292
Size: 188 3774
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2621 3962 1860
Size: 761 1341 1860
Fragments arranged by size:
1860 1341 761
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglII A'GATC_T
Cuts at: 1477 3514 1477
Size: 2037 1925
BlpI GC'TnA_GC
Cuts at: 1074 1074
Size: 3962
Bme1580I G_kGCm'C
Cuts at: 194 314 337 547 1587 1680 2736 194
Size: 120 23 210 1040 93 1056 1420
Fragments arranged by size:
1420 1056 1040 210 120 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 688 775 944 1174 1255 1590 3734 688
Size: 87 169 230 81 335 2144 916
Fragments arranged by size:
2144 916 335 230 169 87 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 831 2329 831
Size: 1498 2464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 607 232
Size: 375 3587
Bpu10I CC'TnA_GC
Cuts at: 292 480 816 292
Size: 188 336 3438
Fragments arranged by size:
3438 336 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 847 1138 2017 2509 2807 640
Size: 207 291 879 492 298 1795
Fragments arranged by size:
1795 879 492 298 291 207
BsaI GGTCTCn'nnnn_
Cuts at: 878 3666 878
Size: 2788 1174
BsaAI yAC'GTr
Cuts at: 390 519 1315 390
Size: 129 796 3037
Fragments arranged by size:
3037 796 129
BsaBI GATnn'nnATC
Cuts at: 6 436 1476 3513 6
Size: 430 1040 2037 455
Fragments arranged by size:
2037 1040 455 430
BsaHI Gr'CG_yC
Cuts at: 179 1393 1641 2343 3839 179
Size: 1214 248 702 1496 302
Fragments arranged by size:
1496 1214 702 302 248
BsaWI w'CCGG_w
Cuts at: 1151 1672 2350 2624 2771 3182 1151
Size: 521 678 274 147 411 1931
Fragments arranged by size:
1931 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 796 826 796
Size: 30 3932
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 246 745 820 124
Size: 122 499 75 3266
Fragments arranged by size:
3266 499 122 75
BseYI C'CCAG_C
Cuts at: 127 314 1387 2145 2722 3218 127
Size: 187 1073 758 577 496 871
Fragments arranged by size:
1073 871 758 577 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 659
Size: 3962
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2758 104
Size: 1400 46 1208 1308
Fragments arranged by size:
1400 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 817 976 1754 1944 2736 817
Size: 159 778 190 792 2043
Fragments arranged by size:
2043 792 778 190 159
Bsp1286I G_dGCh'C
Cuts at: 194 247 314 337 415 547 579 817 976
Size: 53 67 23 78 132 32 238 159
Cuts at: 976 1587 1680 1754 1944 2006 2736 194
Size: 611 93 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 238 190 159 132 93
78 74 67 62 53 32 23
BspEI T'CCGG_A
Cuts at: 3182 3182
Size: 3962
BspHI T'CATG_A
Cuts at: 3138 3138
Size: 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 1226 1528 1909 1012
Size: 214 302 381 3065
Fragments arranged by size:
3065 381 302 214
BsrBI CCG'CTC
Cuts at: 50 185 2254 2308 3792 3957 50
Size: 135 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 844 1960 2141 2350 844
Size: 1116 181 209 2456
Fragments arranged by size:
2456 1116 209 181
BssSI C'ACGA_G
Cuts at: 2233 2591 2233
Size: 358 3604
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3962
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstXI CCAn_nnnn'nTGG
Cuts at: 210 306 327 357 705 772 210
Size: 96 21 30 348 67 3400
Fragments arranged by size:
3400 348 96 67 30 21
BstYI r'GATC_y
Cuts at: 1 987 1454 1477 1812 2058 3070 3156 3168
Size: 986 467 23 335 246 1012 86 12
Cuts at: 3168 3514 1
Size: 346 449
Fragments arranged by size:
1012 986 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 449 107
Size: 342 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1912 2093 2256 1912
Size: 181 163 3618
Fragments arranged by size:
3618 181 163
BtsI GCAGTG_nn'
Cuts at: 945 1205 945
Size: 260 3702
ClaI AT'CG_AT
Cuts at: 304 304
Size: 3962
DraI TTT'AAA
Cuts at: 961 961
Size: 3962
DrdI GACnn_nn'nnGTC
Cuts at: 760 908 1668 2526 760
Size: 148 760 858 2196
Fragments arranged by size:
2196 858 760 148
EaeI y'GGCC_r
Cuts at: 638 842 1547 1721 2112 2139 3484 638
Size: 204 705 174 391 27 1345 1116
Fragments arranged by size:
1345 1116 705 391 204 174 27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3962
EarI CTCTTCn'nnn_
Cuts at: 722 1985 2195 722
Size: 1263 210 2489
Fragments arranged by size:
2489 1263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 2170 2480 2626 3772 370
Size: 1800 310 146 1146 560
Fragments arranged by size:
1800 1146 560 310 146
EcoICRI GAG'CTC
Cuts at: 974 974
Size: 3962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 232 540 607 1787 2219 2966 3234 217
Size: 15 308 67 1180 432 747 268 945
Fragments arranged by size:
1180 945 747 432 308 268 67 15
EcoO109I rG'GnC_Cy
Cuts at: 310 311 333 543 544 564 777 822 910
Size: 1 22 210 1 20 213 45 88
Cuts at: 910 1101 310
Size: 191 3171
Fragments arranged by size:
3171 213 210 191 88 45 22 20
1 1
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 1396 1644 2666 3645 1396
Size: 248 1022 979 1713
Fragments arranged by size:
1713 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 232 264 761 793 1804 1834 1836
Size: 32 31 32 497 32 1011 30 2
Cuts at: 1836 1866 169
Size: 30 2265
Fragments arranged by size:
2265 1011 497 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2817 2633
Size: 184 3778
MscI TGG'CCA
Cuts at: 1723 1723
Size: 3962
MslI CAynn'nnrTG
Cuts at: 304 950 1951 2078 304
Size: 646 1001 127 2188
Fragments arranged by size:
2188 1001 646 127
MspA1I CmG'CkG
Cuts at: 451 1073 1387 1747 2760 3005 451
Size: 622 314 360 1013 245 1408
Fragments arranged by size:
1408 1013 622 360 314 245
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 107 107
Size: 3962
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 1015 1515 2422 1015
Size: 500 907 2555
Fragments arranged by size:
2555 907 500
PasI CC'CwG_GG
Cuts at: 327 897 327
Size: 570 3392
PciI A'CATG_T
Cuts at: 2418 2418
Size: 3962
PfoI T'CCnGG_A
Cuts at: 209 356 209
Size: 147 3815
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 3930
PpuMI rG'GwC_Cy
Cuts at: 564 777 822 910 564
Size: 213 45 88 3616
Fragments arranged by size:
3616 213 88 45
PspOMI G'GGCC_C
Cuts at: 310 333 543 310
Size: 23 210 3729
Fragments arranged by size:
3729 210 23
PstI C_TGCA'G
Cuts at: 1009 1694 1009
Size: 685 3277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 1387 1747 1387
Size: 360 3602
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 976 976
Size: 3962
SacII CC_GC'GG
Cuts at: 452 452
Size: 3962
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SanDI GG'GwC_CC
Cuts at: 777 910 777
Size: 133 3829
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 3479 3479
Size: 3962
SfcI C'TryA_G
Cuts at: 33 1005 1690 2683 2874 33
Size: 972 685 993 191 1121
Fragments arranged by size:
1121 993 972 685 191
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 550 984 550
Size: 434 3528
SmlI C'TyrA_G
Cuts at: 655 826 1117 2032 2524 2786 655
Size: 171 291 915 492 262 1831
Fragments arranged by size:
1831 915 492 291 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
StuI AGG'CCT
Cuts at: 478 478
Size: 3962
StyI C'CwwG_G
Cuts at: 107 855 1096 1450 107
Size: 748 241 354 2619
Fragments arranged by size:
2619 748 354 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 1545 1678 2137 2346 661
Size: 884 133 459 209 2277
Fragments arranged by size:
2277 884 459 209 133
TatI w'GTAC_w
Cuts at: 3477 3477
Size: 3962
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 796 828 3479 3511 796
Size: 32 2651 32 1247
Fragments arranged by size:
2651 1247 32 32
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 354 675 1457 354
Size: 321 782 2859
Fragments arranged by size:
2859 782 321
XmaI C'CCGG_G
Cuts at: 548 982 548
Size: 434 3528
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI