Restriction map for
pF1KB7119
(Circular) (Six-base) MAPSORT of: pF1KB7119.seq Check: 5736 from: 1 to: 3974
pF1KB7119 3974 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 882 882
Size: 3974
AccI GT'mk_AC
Cuts at: 866 1012 866
Size: 146 3828
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 3814 3814
Size: 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1799 2231 2978 3246 1799
Size: 432 747 268 2527
Fragments arranged by size:
2527 747 432 268
AflIII A'CryG_T
Cuts at: 644 668 1326 2430 3407 644
Size: 24 658 1104 977 1211
Fragments arranged by size:
1211 1104 977 658 24
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 193 225 193
Size: 32 3942
AlwNI CAG_nnn'CTG
Cuts at: 279 1279 2846 279
Size: 1000 1567 1407
Fragments arranged by size:
1567 1407 1000
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 978 3327 3583 978
Size: 2349 256 1369
Fragments arranged by size:
2349 1369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 392 624 994 392
Size: 232 370 3372
Fragments arranged by size:
3372 370 232
BamHI G'GATC_C
Cuts at: 999 999
Size: 3974
BanI G'GyrC_C
Cuts at: 338 990 1404 1652 1687 338
Size: 652 414 248 35 2625
Fragments arranged by size:
2625 652 414 248 35
BanII G_rGCy'C
Cuts at: 788 848 988 2018 788
Size: 60 140 1030 2744
Fragments arranged by size:
2744 1030 140 60
BbeI G_GCGC'C
Cuts at: 342 1408 1656 342
Size: 1066 248 2660
Fragments arranged by size:
2660 1066 248
BbvCI CC'TCA_GC
Cuts at: 343 449 343
Size: 106 3868
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BglI GCCn_nnn'nGGC
Cuts at: 388 388
Size: 3974
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 436 1086 436
Size: 650 3324
Bme1580I G_kGCm'C
Cuts at: 1599 1692 2748 1599
Size: 93 1056 2825
Fragments arranged by size:
2825 1056 93
BmgBI CAC'GTC
Cuts at: 291 329 669 696 291
Size: 38 340 27 3569
Fragments arranged by size:
3569 340 38 27
BmrI ACTGGGnnnn_n'
Cuts at: 1186 1267 1602 3746 1186
Size: 81 335 2144 1414
Fragments arranged by size:
2144 1414 335 81
BmtI G_CTAG'C
Cuts at: 124 124
Size: 3974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2341 204
Size: 2137 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 622 778 250
Size: 372 156 3446
Fragments arranged by size:
3446 372 156
Bpu10I CC'TnA_GC
Cuts at: 343 449 343
Size: 106 3868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1150 2029 2521 2819 949
Size: 201 879 492 298 2104
Fragments arranged by size:
2104 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 1327 1327
Size: 3974
BsaBI GATnn'nnATC
Cuts at: 6 586 1488 3525 6
Size: 580 902 2037 455
Fragments arranged by size:
2037 902 580 455
BsaHI Gr'CG_yC
Cuts at: 339 1405 1653 2355 3851 339
Size: 1066 248 702 1496 462
Fragments arranged by size:
1496 1066 702 462 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 352 382 223
Size: 30 99 30 3815
Fragments arranged by size:
3815 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 931 931
Size: 3974
BseYI C'CCAG_C
Cuts at: 300 455 1399 2157 2734 3230 300
Size: 155 944 758 577 496 1044
Fragments arranged by size:
1044 944 758 577 496 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 3974
BsiEI CG_ry'CG
Cuts at: 104 866 1516 1562 2770 104
Size: 762 650 46 1208 1308
Fragments arranged by size:
1308 1208 762 650 46
BsiHKAI G_wGCw'C
Cuts at: 788 848 988 1766 1956 2748 788
Size: 60 140 778 190 792 2014
Fragments arranged by size:
2014 792 778 190 140 60
BsmBI CGTCTCn'nnnn_
Cuts at: 208 208
Size: 3974
Bsp1286I G_dGCh'C
Cuts at: 788 848 988 1599 1692 1766 1956 2018 2748
Size: 60 140 611 93 74 190 62 730
Cuts at: 2748 788
Size: 2014
Fragments arranged by size:
2014 730 611 190 140 93 74 62
60
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 882 1024 1238 1540 1921 882
Size: 142 214 302 381 2935
Fragments arranged by size:
2935 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 2266 2320 3804 3969 50
Size: 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 116 874 1972 2153 2362 116
Size: 758 1098 181 209 1728
Fragments arranged by size:
1728 1098 758 209 181
BssHII G'CGCG_C
Cuts at: 420 481 528 704 420
Size: 61 47 176 3690
Fragments arranged by size:
3690 176 61 47
BssSI C'ACGA_G
Cuts at: 196 781 2245 2603 196
Size: 585 1464 358 1567
Fragments arranged by size:
1567 1464 585 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1351 1351
Size: 3974
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstYI r'GATC_y
Cuts at: 1 581 999 1466 1489 1824 2070 3082 3168
Size: 580 418 467 23 335 246 1012 86
Cuts at: 3168 3180 3526 1
Size: 12 346 449
Fragments arranged by size:
1012 580 467 449 418 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 357 1924 2105 2268 357
Size: 1567 181 163 2063
Fragments arranged by size:
2063 1567 181 163
BtsI GCAGTG_nn'
Cuts at: 408 482 1217 408
Size: 74 735 3165
Fragments arranged by size:
3165 735 74
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DrdI GACnn_nn'nnGTC
Cuts at: 1680 2538 1680
Size: 858 3116
EaeI y'GGCC_r
Cuts at: 284 689 876 1559 1733 2124 2151 3496 284
Size: 405 187 683 174 391 27 1345 762
Fragments arranged by size:
1345 762 683 405 391 187 174 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 793 1997 2207 793
Size: 1204 210 2560
Fragments arranged by size:
2560 1204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2182 2492 2638 3784 2182
Size: 310 146 1146 2372
Fragments arranged by size:
2372 1146 310 146
EcoICRI GAG'CTC
Cuts at: 786 846 986 786
Size: 60 140 3774
Fragments arranged by size:
3774 140 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 622 778 1799 2231 2978 3246 250
Size: 372 156 1021 432 747 268 978
Fragments arranged by size:
1021 978 747 432 372 268 156
EcoO109I rG'GnC_Cy
Cuts at: 1113 1113
Size: 3974
EcoRI G'AATT_C
Cuts at: 978 978
Size: 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 342 597 1408 1656 2678 3657 342
Size: 255 811 248 1022 979 659
Fragments arranged by size:
1022 979 811 659 255 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 1816 1846 1848 1878 352
Size: 32 1432 30 2 30 2448
Fragments arranged by size:
2448 1432 32 30 30 2
HincII GTy'rAC
Cuts at: 867 1013 867
Size: 146 3828
KasI G'GCGC_C
Cuts at: 338 1404 1652 338
Size: 1066 248 2660
Fragments arranged by size:
2660 1066 248
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2829 2645
Size: 184 3790
MscI TGG'CCA
Cuts at: 1735 1735
Size: 3974
MslI CAynn'nnrTG
Cuts at: 331 1963 2090 331
Size: 1632 127 2215
Fragments arranged by size:
2215 1632 127
MspA1I CmG'CkG
Cuts at: 276 600 1085 1399 1759 2772 3017 276
Size: 324 485 314 360 1013 245 1233
Fragments arranged by size:
1233 1013 485 360 324 314 245
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 3974
NarI GG'CG_CC
Cuts at: 339 1405 1653 339
Size: 1066 248 2660
Fragments arranged by size:
2660 1066 248
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 3974
NheI G'CTAG_C
Cuts at: 120 120
Size: 3974
NspI r_CATG'y
Cuts at: 648 1027 1527 2434 648
Size: 379 500 907 2188
Fragments arranged by size:
2188 907 500 379
PciI A'CATG_T
Cuts at: 644 2430 644
Size: 1786 2188
PflMI CCAn_nnn'nTGG
Cuts at: 228 279 228
Size: 51 3923
PfoI T'CCnGG_A
Cuts at: 227 251 227
Size: 24 3950
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3113 3145 631
Size: 32 2450 32 1460
Fragments arranged by size:
2450 1460 32 32
PstI C_TGCA'G
Cuts at: 1021 1706 1021
Size: 685 3289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3974
PvuII CAG'CTG
Cuts at: 276 600 1399 1759 276
Size: 324 799 360 2491
Fragments arranged by size:
2491 799 360 324
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 788 848 988 788
Size: 60 140 3774
Fragments arranged by size:
3774 140 60
SalI G'TCGA_C
Cuts at: 865 1011 865
Size: 146 3828
SapI GCTCTTCn'nnn_
Cuts at: 793 1997 2207 793
Size: 1204 210 2560
Fragments arranged by size:
2560 1204 210
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SfcI C'TryA_G
Cuts at: 33 1017 1702 2695 2886 33
Size: 984 685 993 191 1121
Fragments arranged by size:
1121 993 984 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 388 388
Size: 3974
SfoI GGC'GCC
Cuts at: 340 1406 1654 340
Size: 1066 248 2660
Fragments arranged by size:
2660 1066 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 394 996 394
Size: 602 3372
SmlI C'TyrA_G
Cuts at: 964 1129 2044 2536 2798 964
Size: 165 915 492 262 2140
Fragments arranged by size:
2140 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 1027 1527 1027
Size: 500 3474
StyI C'CwwG_G
Cuts at: 377 884 1108 1462 377
Size: 507 224 354 2889
Fragments arranged by size:
2889 507 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 500 3489 500
Size: 2989 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 3942
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1469 1469
Size: 3974
XmaI C'CCGG_G
Cuts at: 392 994 392
Size: 602 3372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BsiWI BsmI BsrGI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI