Restriction map for
pF1KB7120
(Circular) (Six-base) MAPSORT of: pF1KB7120.seq Check: 1193 from: 1 to: 3995
pF1KB7120 3995 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 366 382 1033 366
Size: 16 651 3328
Fragments arranged by size:
3328 651 16
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 662 1820 2252 2999 3267 470
Size: 192 1158 432 747 268 1198
Fragments arranged by size:
1198 1158 747 432 268 192
AflII C'TTAA_G
Cuts at: 370 370
Size: 3995
AflIII A'CryG_T
Cuts at: 1347 2451 3428 1347
Size: 1104 977 1914
Fragments arranged by size:
1914 1104 977
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 1300 2867 1300
Size: 1567 2428
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 316 404 907 999 3348 3604 316
Size: 88 503 92 2349 256 707
Fragments arranged by size:
2349 707 503 256 92 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 1015 1015
Size: 3995
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 1011 1425 1673 1708 1011
Size: 414 248 35 3298
Fragments arranged by size:
3298 414 248 35
BanII G_rGCy'C
Cuts at: 1009 2039 1009
Size: 1030 2965
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BciVI GTATCCnnnnn_n'
Cuts at: 494 1893 2654 3995 494
Size: 1399 761 1341 494
Fragments arranged by size:
1399 1341 761 494
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglII A'GATC_T
Cuts at: 1510 3547 1510
Size: 2037 1958
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 1620 1713 2769 1620
Size: 93 1056 2846
Fragments arranged by size:
2846 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1207 1288 1623 3767 1207
Size: 81 335 2144 1435
Fragments arranged by size:
2144 1435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 3995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2050 2542 2840 1171
Size: 879 492 298 2326
Fragments arranged by size:
2326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3699 3699
Size: 3995
BsaAI yAC'GTr
Cuts at: 1348 1348
Size: 3995
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 1426 1674 2376 3872 1426
Size: 248 702 1496 1549
Fragments arranged by size:
1549 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1184 1705 2383 2657 2804 3215 1184
Size: 521 678 274 147 411 1964
Fragments arranged by size:
1964 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1420 2178 2755 3251 1420
Size: 758 577 496 2164
Fragments arranged by size:
2164 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1009 1787 1977 2769 1009
Size: 778 190 792 2235
Fragments arranged by size:
2235 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1009 1620 1713 1787 1977 2039 2769 1009
Size: 611 93 74 190 62 730 2235
Fragments arranged by size:
2235 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3215 3215
Size: 3995
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1259 1561 1942 1045
Size: 214 302 381 3098
Fragments arranged by size:
3098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2287 2341 3825 3990 50
Size: 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 1907 530
Size: 1377 2618
BsrFI r'CCGG_y
Cuts at: 1993 2174 2383 1993
Size: 181 209 3605
Fragments arranged by size:
3605 209 181
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 1372 1372
Size: 3995
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstYI r'GATC_y
Cuts at: 1 798 1020 1487 1510 1845 2091 3103 3189
Size: 797 222 467 23 335 246 1012 86
Cuts at: 3189 3201 3547 1
Size: 12 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 222
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 1238 1238
Size: 3995
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 1701 2559 1701
Size: 858 3137
EaeI y'GGCC_r
Cuts at: 1580 1754 2145 2172 3517 1580
Size: 174 391 27 1345 2058
Fragments arranged by size:
2058 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 520 2203 2513 2659 3805 520
Size: 1683 310 146 1146 710
Fragments arranged by size:
1683 1146 710 310 146
EcoICRI GAG'CTC
Cuts at: 1007 1007
Size: 3995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 662 1820 2252 2999 3267 470
Size: 192 1158 432 747 268 1198
Fragments arranged by size:
1198 1158 747 432 268 192
EcoO109I rG'GnC_Cy
Cuts at: 1134 1134
Size: 3995
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
EcoRV GAT'ATC
Cuts at: 815 815
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 136 748 959 2014 136
Size: 612 211 1055 2117
Fragments arranged by size:
2117 1055 612 211
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 1429 1677 2699 3678 1429
Size: 248 1022 979 1746
Fragments arranged by size:
1746 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 830 862 1837 1867 1869 1899 830
Size: 32 975 30 2 30 2926
Fragments arranged by size:
2926 975 32 30 30 2
HincII GTy'rAC
Cuts at: 891 1034 891
Size: 143 3852
HindIII A'AGCT_T
Cuts at: 696 696
Size: 3995
HpaI GTT'AAC
Cuts at: 891 891
Size: 3995
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 1756 1756
Size: 3995
MslI CAynn'nnrTG
Cuts at: 260 1984 2111 260
Size: 1724 127 2144
Fragments arranged by size:
2144 1724 127
MspA1I CmG'CkG
Cuts at: 1106 1420 1780 2793 3038 1106
Size: 314 360 1013 245 2063
Fragments arranged by size:
2063 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 520 1048 1548 2455 520
Size: 528 500 907 2060
Fragments arranged by size:
2060 907 528 500
PciI A'CATG_T
Cuts at: 2451 2451
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PstI C_TGCA'G
Cuts at: 1042 1727 1042
Size: 685 3310
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 1420 1780 1420
Size: 360 3635
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 1009 1009
Size: 3995
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 953 3512 953
Size: 2559 1436
SfcI C'TryA_G
Cuts at: 33 182 211 242 277 978 1038 1723 2716
Size: 149 29 31 35 701 60 685 993
Cuts at: 2716 2907 33
Size: 191 1121
Fragments arranged by size:
1121 993 701 685 191 149 60 35
31 29
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 1017 1017
Size: 3995
SmlI C'TyrA_G
Cuts at: 370 1150 2065 2557 2819 370
Size: 780 915 492 262 1546
Fragments arranged by size:
1546 915 780 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 520 1048 1548 520
Size: 528 500 2967
Fragments arranged by size:
2967 528 500
SspI AAT'ATT
Cuts at: 855 855
Size: 3995
StyI C'CwwG_G
Cuts at: 1129 1483 1129
Size: 354 3641
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 1578 1711 2170 2379 131
Size: 1447 133 459 209 1747
Fragments arranged by size:
1747 1447 459 209 133
TatI w'GTAC_w
Cuts at: 874 951 3510 874
Size: 77 2559 1359
Fragments arranged by size:
2559 1359 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 3963
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 160 1026 160
Size: 866 3129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1490 1490
Size: 3995
XmaI C'CCGG_G
Cuts at: 1015 1015
Size: 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI