Restriction map for
pF1KB7138
(Circular) (Six-base) MAPSORT of: pF1KB7138.seq Check: 9458 from: 1 to: 4106
pF1KB7138 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 628 816 628
Size: 188 3918
AatII G_ACGT'C
Cuts at: 766 766
Size: 4106
AccI GT'mk_AC
Cuts at: 1065 1144 1065
Size: 79 4027
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 3110 3378 1931
Size: 432 747 268 2659
Fragments arranged by size:
2659 747 432 268
AflIII A'CryG_T
Cuts at: 509 943 1458 2562 3539 509
Size: 434 515 1104 977 1076
Fragments arranged by size:
1104 1076 977 515 434
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 1411 2978 1411
Size: 1567 2539
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 992 1126 992
Size: 134 3972
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 405 1120 2150 405
Size: 715 1030 2361
Fragments arranged by size:
2361 1030 715
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 180 180
Size: 4106
BbvCI CC'TCA_GC
Cuts at: 937 937
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 110 1626 110
Size: 1516 2590
BglI GCCn_nnn'nGGC
Cuts at: 526 526
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2880 1731
Size: 93 1056 2957
Fragments arranged by size:
2957 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
Bpu10I CC'TnA_GC
Cuts at: 937 937
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1282 2161 2653 2951 610
Size: 672 879 492 298 1765
Fragments arranged by size:
1765 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 512 944 1459 512
Size: 432 515 3159
Fragments arranged by size:
3159 515 432
BsaBI GATnn'nnATC
Cuts at: 6 808 1620 3657 6
Size: 802 812 2037 455
Fragments arranged by size:
2037 812 802 455
BsaHI Gr'CG_yC
Cuts at: 763 1537 1785 2487 3983 763
Size: 774 248 702 1496 886
Fragments arranged by size:
1496 886 774 702 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 876 1531 2289 2866 3362 876
Size: 655 758 577 496 1620
Fragments arranged by size:
1620 758 655 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 844
Size: 4106
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 405 833 1120 1898 2088 2880 405
Size: 428 287 778 190 792 1631
Fragments arranged by size:
1631 792 778 428 287 190
Bsp1286I G_dGCh'C
Cuts at: 405 833 1120 1731 1824 1898 2088 2150 2880
Size: 428 287 611 93 74 190 62 730
Cuts at: 2880 405
Size: 1631
Fragments arranged by size:
1631 730 611 428 287 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 713 929 3282 713
Size: 216 2353 1537
Fragments arranged by size:
2353 1537 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 816 1156 1370 1672 2053 628
Size: 188 340 214 302 381 2681
Fragments arranged by size:
2681 381 340 302 214 188
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 2018 425
Size: 1593 2513
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BsrGI T'GTAC_A
Cuts at: 477 477
Size: 4106
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 339 1029 2467 339
Size: 690 1438 1978
Fragments arranged by size:
1978 1438 690
BstEII G'GTnAC_C
Cuts at: 276 276
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 397 397
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 3214 3300 3312
Size: 1130 467 23 335 246 1012 86 12
Cuts at: 3312 3658 1
Size: 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 272 272
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 230 1349 230
Size: 1119 2987
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 367 470 1691 1865 2256 2283 3628 367
Size: 103 1221 174 391 27 1345 845
Fragments arranged by size:
1345 1221 845 391 174 103 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 259 2129 2339 259
Size: 1870 210 2026
Fragments arranged by size:
2026 1870 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 403 1118 403
Size: 715 3391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 3110 3378 1931
Size: 432 747 268 2659
Fragments arranged by size:
2659 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1245 1245
Size: 4106
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 446 2125 446
Size: 1679 2427
FspI TGC'GCA
Cuts at: 1039 1887 1039
Size: 848 3258
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 589 621 1948 1978 1980 2010 555
Size: 32 2 32 1327 30 2 30 2651
Fragments arranged by size:
2651 1327 32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 225 1145 225
Size: 920 3186
HpaI GTT'AAC
Cuts at: 225 225
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 369 472 1867 369
Size: 103 1395 2608
Fragments arranged by size:
2608 1395 103
MslI CAynn'nnrTG
Cuts at: 161 286 2095 2222 161
Size: 125 1809 127 2045
Fragments arranged by size:
2045 1809 127 125
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 1038 1159 1659 2566 1038
Size: 121 500 907 2578
Fragments arranged by size:
2578 907 500 121
PasI CC'CwG_GG
Cuts at: 905 905
Size: 4106
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PmlI CAC'GTG
Cuts at: 512 944 512
Size: 432 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PsiI TTA'TAA
Cuts at: 322 322
Size: 4106
PspXI vC'TCGA_Gb
Cuts at: 992 992
Size: 4106
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 405 1120 405
Size: 715 3391
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 382 3623 382
Size: 3241 865
SfcI C'TryA_G
Cuts at: 33 192 1149 1834 2827 3018 33
Size: 159 957 685 993 191 1121
Fragments arranged by size:
1121 993 957 685 191 159
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 625 992 1261 2176 2668 2930 625
Size: 367 269 915 492 262 1801
Fragments arranged by size:
1801 915 492 367 269 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1038 1159 1659 1038
Size: 121 500 3485
Fragments arranged by size:
3485 500 121
StyI C'CwwG_G
Cuts at: 1240 1594 1240
Size: 354 3752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 710 1689 1822 2281 2490 710
Size: 979 133 459 209 2326
Fragments arranged by size:
2326 979 459 209 133
TatI w'GTAC_w
Cuts at: 380 477 3621 380
Size: 97 3144 865
Fragments arranged by size:
3144 865 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XhoI C'TCGA_G
Cuts at: 992 992
Size: 4106
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
ZraI GAC'GTC
Cuts at: 764 764
Size: 4106
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BpmI BsaXI BseRI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PpuMI PshAI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI