Restriction map for
pF1KB7140
(Circular) (Six-base) MAPSORT of: pF1KB7140.seq Check: 4055 from: 1 to: 4112
pF1KB7140 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 912 1150 912
Size: 238 3874
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AleI CACnn'nnGTG
Cuts at: 118 301 118
Size: 183 3929
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 1012 1116 3465 3721 1012
Size: 104 2349 256 1403
Fragments arranged by size:
2349 1403 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 981 1137 981
Size: 156 3956
BanI G'GyrC_C
Cuts at: 149 363 1128 1542 1790 1825 149
Size: 214 765 414 248 35 2436
Fragments arranged by size:
2436 765 414 248 214 35
BanII G_rGCy'C
Cuts at: 452 1126 2156 452
Size: 674 1030 2408
Fragments arranged by size:
2408 1030 674
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 825 825
Size: 4112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 590 624 590
Size: 34 4078
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 648 648
Size: 4112
BglII A'GATC_T
Cuts at: 139 1627 3664 139
Size: 1488 2037 587
Fragments arranged by size:
2037 1488 587
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 154 1737 1830 2886 154
Size: 1583 93 1056 1380
Fragments arranged by size:
1583 1380 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 606 1288 2167 2659 2957 457
Size: 149 682 879 492 298 1612
Fragments arranged by size:
1612 879 682 492 298 149
BsaI GGTCTCn'nnnn_
Cuts at: 412 537 3816 412
Size: 125 3279 708
Fragments arranged by size:
3279 708 125
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 258 258
Size: 4112
BseYI C'CCAG_C
Cuts at: 186 262 1537 2295 2872 3368 186
Size: 76 1275 758 577 496 930
Fragments arranged by size:
1275 930 758 577 496 76
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 154 452 1126 1737 1830 1904 2094 2156 2886
Size: 298 674 611 93 74 190 62 730
Cuts at: 2886 154
Size: 1380
Fragments arranged by size:
1380 730 674 611 298 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 147 2404 2458 3942 4107 50
Size: 97 2257 54 1484 165 55
Fragments arranged by size:
2257 1484 165 97 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 214 2110 2291 2500 214
Size: 1896 181 209 1826
Fragments arranged by size:
1896 1826 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 429 429
Size: 4112
BstYI r'GATC_y
Cuts at: 1 139 981 1137 1604 1627 1962 2208 3220
Size: 138 842 156 467 23 335 246 1012
Cuts at: 3220 3306 3318 3664 1
Size: 86 12 346 449
Fragments arranged by size:
1012 842 467 449 346 335 246 156
138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1101 1101
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 305 1697 1871 2262 2289 3634 305
Size: 1392 174 391 27 1345 783
Fragments arranged by size:
1392 1345 783 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 640 1251 640
Size: 611 3501
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 118 121 301 427 976 2101 2228 118
Size: 3 180 126 549 1125 127 2002
Fragments arranged by size:
2002 1125 549 180 127 126 3
MspA1I CmG'CkG
Cuts at: 177 1223 1537 1897 2910 3155 177
Size: 1046 314 360 1013 245 1134
Fragments arranged by size:
1134 1046 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NdeI CA'TA_TG
Cuts at: 679 679
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 177 1537 1897 177
Size: 1360 360 2392
Fragments arranged by size:
2392 1360 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 909 1155 1840 2833 3024 33
Size: 876 246 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 246 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 472 585 1267 2182 2674 2936 472
Size: 113 682 915 492 262 1648
Fragments arranged by size:
1648 915 682 492 262 113
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
SspI AAT'ATT
Cuts at: 498 498
Size: 4112
StyI C'CwwG_G
Cuts at: 1246 1600 1246
Size: 354 3758
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 1695 1828 2287 2496 234
Size: 1461 133 459 209 1850
Fragments arranged by size:
1850 1461 459 209 133
TatI w'GTAC_w
Cuts at: 171 3627 171
Size: 3456 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI