Restriction map for
pF1KB7144
(Circular) (Six-base) MAPSORT of: pF1KB7144.seq Check: 8416 from: 1 to: 4130
pF1KB7144 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 507 1019 1955 2387 3134 3402 463
Size: 44 512 936 432 747 268 1191
Fragments arranged by size:
1191 936 747 512 432 268 44
AflIII A'CryG_T
Cuts at: 455 1482 2586 3563 455
Size: 1027 1104 977 1022
Fragments arranged by size:
1104 1027 1022 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AleI CACnn'nnGTG
Cuts at: 193 193
Size: 4130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 695 1011 695
Size: 316 3814
AlwNI CAG_nnn'CTG
Cuts at: 536 712 1435 3002 536
Size: 176 723 1567 1664
Fragments arranged by size:
1664 1567 723 176
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 199 1134 3483 3739 199
Size: 935 2349 256 590
Fragments arranged by size:
2349 935 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 1150 1150
Size: 4130
BamHI G'GATC_C
Cuts at: 1008 1155 1008
Size: 147 3983
BanI G'GyrC_C
Cuts at: 1146 1560 1808 1843 1146
Size: 414 248 35 3433
Fragments arranged by size:
3433 414 248 35
BanII G_rGCy'C
Cuts at: 420 1144 2174 420
Size: 724 1030 2376
Fragments arranged by size:
2376 1030 724
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 244 484 852 244
Size: 240 368 3522
Fragments arranged by size:
3522 368 240
BciVI GTATCCnnnnn_n'
Cuts at: 658 2028 2789 4130 658
Size: 1370 761 1341 658
Fragments arranged by size:
1370 1341 761 658
BclI T'GATC_A
Cuts at: 326 740 1650 326
Size: 414 910 2806
Fragments arranged by size:
2806 910 414
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 713 1242 713
Size: 529 3601
Bme1580I G_kGCm'C
Cuts at: 1755 1848 2904 1755
Size: 93 1056 2981
Fragments arranged by size:
2981 1056 93
BmgBI CAC'GTC
Cuts at: 482 482
Size: 4130
BmrI ACTGGGnnnn_n'
Cuts at: 730 1342 1423 1758 3902 730
Size: 612 81 335 2144 958
Fragments arranged by size:
2144 958 612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 801 1306 2185 2677 2975 499
Size: 302 505 879 492 298 1654
Fragments arranged by size:
1654 879 505 492 302 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 745 1644 3681 6
Size: 739 899 2037 455
Fragments arranged by size:
2037 899 739 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 374 1319 1840 2518 2792 2939 3350 374
Size: 945 521 678 274 147 411 1154
Fragments arranged by size:
1154 945 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 502
Size: 4130
BseYI C'CCAG_C
Cuts at: 1555 2313 2890 3386 1555
Size: 758 577 496 2299
Fragments arranged by size:
2299 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 287
Size: 4130
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2926 104
Size: 1568 46 1208 1308
Fragments arranged by size:
1568 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1144 1922 2112 2904 1144
Size: 778 190 792 2370
Fragments arranged by size:
2370 792 778 190
BsmI GAATG_Cn'
Cuts at: 684 684
Size: 4130
Bsp1286I G_dGCh'C
Cuts at: 420 1144 1755 1848 1922 2112 2174 2904 420
Size: 724 611 93 74 190 62 730 1646
Fragments arranged by size:
1646 730 724 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 374 3350 374
Size: 2976 1154
BspHI T'CATG_A
Cuts at: 323 1091 3306 323
Size: 768 2215 1147
Fragments arranged by size:
2215 1147 768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1082 2422 2476 3960 4125 50
Size: 1032 1340 54 1484 165 55
Fragments arranged by size:
1484 1340 1032 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 2128 2309 2518 2128
Size: 181 209 3740
Fragments arranged by size:
3740 209 181
BssSI C'ACGA_G
Cuts at: 2401 2759 2401
Size: 358 3772
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 1008 1155 1622 1645 1980 2226 3238 3324
Size: 1007 147 467 23 335 246 1012 86
Cuts at: 3324 3336 3682 1
Size: 12 346 449
Fragments arranged by size:
1012 1007 467 449 346 335 246 147
86 23 12
BtgI C'CryG_G
Cuts at: 107 908 1062 107
Size: 801 154 3175
Fragments arranged by size:
3175 801 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 732 2080 2261 2424 732
Size: 1348 181 163 2438
Fragments arranged by size:
2438 1348 181 163
BtsI GCAGTG_nn'
Cuts at: 164 829 1373 164
Size: 665 544 2921
Fragments arranged by size:
2921 665 544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 955 990 1086 1121 955
Size: 35 96 35 3964
Fragments arranged by size:
3964 96 35 35
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 1715 1889 2280 2307 3652 1715
Size: 174 391 27 1345 2193
Fragments arranged by size:
2193 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 507 1019 1955 2387 3134 3402 463
Size: 44 512 936 432 747 268 1191
Fragments arranged by size:
1191 936 747 512 432 268 44
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 666 2149 666
Size: 1483 2647
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 2834 3813 1564
Size: 248 1022 979 1881
Fragments arranged by size:
1881 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 716 748 1039 1071 1972 2002 2004 2034 716
Size: 32 291 32 901 30 2 30 2812
Fragments arranged by size:
2812 901 291 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2801 2985 2801
Size: 184 3946
MscI TGG'CCA
Cuts at: 1891 1891
Size: 4130
MslI CAynn'nnrTG
Cuts at: 193 322 2119 2246 193
Size: 129 1797 127 2077
Fragments arranged by size:
2077 1797 129 127
MspA1I CmG'CkG
Cuts at: 1241 1555 1915 2928 3173 1241
Size: 314 360 1013 245 2198
Fragments arranged by size:
2198 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 908 1062 107
Size: 801 154 3175
Fragments arranged by size:
3175 801 154
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NspI r_CATG'y
Cuts at: 459 1183 1683 2590 459
Size: 724 500 907 1999
Fragments arranged by size:
1999 907 724 500
PciI A'CATG_T
Cuts at: 455 2586 455
Size: 2131 1999
PflMI CCAn_nnn'nTGG
Cuts at: 536 536
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PstI C_TGCA'G
Cuts at: 858 1177 1862 858
Size: 319 685 3126
Fragments arranged by size:
3126 685 319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 1555 1915 1555
Size: 360 3770
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 854 1173 1858 2851 3042 33
Size: 821 319 685 993 191 1121
Fragments arranged by size:
1121 993 821 685 319 191
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 514 780 1285 2200 2692 2954 514
Size: 266 505 915 492 262 1690
Fragments arranged by size:
1690 915 505 492 266 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StyI C'CwwG_G
Cuts at: 107 804 908 1062 1264 1618 107
Size: 697 104 154 202 354 2619
Fragments arranged by size:
2619 697 354 202 154 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1713 1846 2305 2514 1713
Size: 133 459 209 3329
Fragments arranged by size:
3329 459 209 133
TatI w'GTAC_w
Cuts at: 3645 3645
Size: 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 3647 3679 947
Size: 32 2668 32 1398
Fragments arranged by size:
2668 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI