Restriction map for
pF1KB7145
(Circular) (Six-base) MAPSORT of: pF1KB7145.seq Check: 9433 from: 1 to: 4145
pF1KB7145 4145 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 464 574 1183 464
Size: 110 609 3426
Fragments arranged by size:
3426 609 110
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1032 1079 1970 2402 3149 3417 306
Size: 726 47 891 432 747 268 1034
Fragments arranged by size:
1034 891 747 726 432 268 47
AflIII A'CryG_T
Cuts at: 404 1497 2601 3578 404
Size: 1093 1104 977 971
Fragments arranged by size:
1104 1093 977 971
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AleI CACnn'nnGTG
Cuts at: 523 523
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 551 2915 551
Size: 2364 1781
ApoI r'AATT_y
Cuts at: 618 1005 1077 1149 3498 3754 618
Size: 387 72 72 2349 256 1009
Fragments arranged by size:
2349 1009 387 256 72 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 1165 1165
Size: 4145
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 1161 1575 1823 1858 1161
Size: 414 248 35 3448
Fragments arranged by size:
3448 414 248 35
BanII G_rGCy'C
Cuts at: 1159 2189 1159
Size: 1030 3115
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 812 1665 812
Size: 853 3292
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 555 1770 1863 2919 555
Size: 1215 93 1056 1781
Fragments arranged by size:
1781 1215 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1438 1773 3917 1357
Size: 81 335 2144 1585
Fragments arranged by size:
2144 1585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 1321 2200 2692 2990 636
Size: 685 879 492 298 1791
Fragments arranged by size:
1791 879 685 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1334 1855 2533 2807 2954 3365 1334
Size: 521 678 274 147 411 2114
Fragments arranged by size:
2114 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4145
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 555 1159 1937 2127 2919 555
Size: 604 778 190 792 1781
Fragments arranged by size:
1781 792 778 604 190
BsmI GAATG_Cn'
Cuts at: 416 416
Size: 4145
Bsp1286I G_dGCh'C
Cuts at: 555 1159 1770 1863 1937 2127 2189 2919 555
Size: 604 611 93 74 190 62 730 1781
Fragments arranged by size:
1781 730 611 604 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1195 1409 1711 2092 1195
Size: 214 302 381 3248
Fragments arranged by size:
3248 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 2057 293
Size: 1764 2381
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BsrGI T'GTAC_A
Cuts at: 804 804
Size: 4145
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstYI r'GATC_y
Cuts at: 1 1170 1637 1660 1995 2241 3253 3339 3351
Size: 1169 467 23 335 246 1012 86 12
Cuts at: 3351 3697 1
Size: 346 449
Fragments arranged by size:
1169 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 129 1388 129
Size: 1259 2886
ClaI AT'CG_AT
Cuts at: 447 447
Size: 4145
DraI TTT'AAA
Cuts at: 704 791 1144 704
Size: 87 353 3705
Fragments arranged by size:
3705 353 87
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 352 1730 1904 2295 2322 3667 352
Size: 1378 174 391 27 1345 830
Fragments arranged by size:
1378 1345 830 391 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1032 1079 1970 2402 3149 3417 306
Size: 726 47 891 432 747 268 1034
Fragments arranged by size:
1034 891 747 726 432 268 47
EcoO109I rG'GnC_Cy
Cuts at: 1284 1284
Size: 4145
EcoRI G'AATT_C
Cuts at: 1005 1149 1005
Size: 144 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 1987 2017 2019 2049 1114
Size: 32 841 30 2 30 3210
Fragments arranged by size:
3210 841 32 30 30 2
HincII GTy'rAC
Cuts at: 120 533 1184 120
Size: 413 651 3081
Fragments arranged by size:
3081 651 413
HpaI GTT'AAC
Cuts at: 120 533 120
Size: 413 3732
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MfeI C'AATT_G
Cuts at: 210 458 210
Size: 248 3897
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 977 2816 3000 977
Size: 1839 184 2122
Fragments arranged by size:
2122 1839 184
MscI TGG'CCA
Cuts at: 354 1906 354
Size: 1552 2593
MslI CAynn'nnrTG
Cuts at: 523 2134 2261 523
Size: 1611 127 2407
Fragments arranged by size:
2407 1611 127
MspA1I CmG'CkG
Cuts at: 1256 1570 1930 2943 3188 1256
Size: 314 360 1013 245 2213
Fragments arranged by size:
2213 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 107
Size: 4145
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 177 408 1198 1698 2605 177
Size: 231 790 500 907 1717
Fragments arranged by size:
1717 907 790 500 231
PciI A'CATG_T
Cuts at: 404 2601 404
Size: 2197 1948
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PstI C_TGCA'G
Cuts at: 1192 1877 1192
Size: 685 3460
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 1570 1930 1570
Size: 360 3785
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 971 1096 1188 1873 2866 3057 33
Size: 938 125 92 685 993 191 1121
Fragments arranged by size:
1121 993 938 685 191 125 92
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 651 1300 2215 2707 2969 651
Size: 649 915 492 262 1827
Fragments arranged by size:
1827 915 649 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StyI C'CwwG_G
Cuts at: 107 1279 1633 107
Size: 1172 354 2619
Fragments arranged by size:
2619 1172 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 158 1728 1861 2320 2529 158
Size: 1570 133 459 209 1774
Fragments arranged by size:
1774 1570 459 209 133
TatI w'GTAC_w
Cuts at: 155 804 3660 155
Size: 649 2856 640
Fragments arranged by size:
2856 649 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4113
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 632 1640 632
Size: 1008 3137
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
XmnI GAAnn'nnTTC
Cuts at: 620 620
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI