Restriction map for
pF1KB7149
(Circular) (Six-base) MAPSORT of: pF1KB7149.seq Check: 7979 from: 1 to: 4154
pF1KB7149 4154 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 873 873
Size: 4154
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 892 1979 2411 3158 3426 532
Size: 360 1087 432 747 268 1260
Fragments arranged by size:
1260 1087 747 432 360 268
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AhdI GACnn_n'nnGTC
Cuts at: 176 176
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 772 1158 3507 3763 772
Size: 386 2349 256 1163
Fragments arranged by size:
2349 1163 386 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 113 964 1174 113
Size: 851 210 3093
Fragments arranged by size:
3093 851 210
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 352 885 988 1170 1584 1832 1867 352
Size: 533 103 182 414 248 35 2639
Fragments arranged by size:
2639 533 414 248 182 103 35
BanII G_rGCy'C
Cuts at: 300 415 491 584 1053 1122 1168 2198 300
Size: 115 76 93 469 69 46 1030 2256
Fragments arranged by size:
2256 1030 469 115 93 76 69 46
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 4154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4120
BciVI GTATCCnnnnn_n'
Cuts at: 240 2052 2813 4154 240
Size: 1812 761 1341 240
Fragments arranged by size:
1812 1341 761 240
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglI GCCn_nnn'nGGC
Cuts at: 439 439
Size: 4154
BglII A'GATC_T
Cuts at: 764 849 1669 3706 764
Size: 85 820 2037 1212
Fragments arranged by size:
2037 1212 820 85
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 223 890 1779 1872 2928 223
Size: 667 889 93 1056 1449
Fragments arranged by size:
1449 1056 889 667 93
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 240 2521 240
Size: 2281 1873
Bpu10I CC'TnA_GC
Cuts at: 673 673
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1330 2209 2701 2999 166
Size: 1164 879 492 298 1321
Fragments arranged by size:
1321 1164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 184 3858 163
Size: 21 3674 459
Fragments arranged by size:
3674 459 21
BsaAI yAC'GTr
Cuts at: 469 1507 469
Size: 1038 3116
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 1080 1585 1833 2535 4031 1080
Size: 505 248 702 1496 1203
Fragments arranged by size:
1496 1203 702 505 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 216 219 229 820 181
Size: 35 3 10 591 3515
Fragments arranged by size:
3515 591 35 10 3
BseYI C'CCAG_C
Cuts at: 1579 2337 2914 3410 1579
Size: 758 577 496 2323
Fragments arranged by size:
2323 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 877
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2950 104
Size: 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 141 300 415 584 812 1168 1946 2136 2928
Size: 159 115 169 228 356 778 190 792
Cuts at: 2928 141
Size: 1367
Fragments arranged by size:
1367 792 778 356 228 190 169 159
115
BsmI GAATG_Cn'
Cuts at: 275 452 275
Size: 177 3977
Bsp1286I G_dGCh'C
Cuts at: 141 223 300 415 491 584 812 890 1053
Size: 82 77 115 76 93 228 78 163
Cuts at: 1053 1122 1168 1779 1872 1946 2136 2198 2928
Size: 69 46 611 93 74 190 62 730
Cuts at: 2928 141
Size: 1367
Fragments arranged by size:
1367 730 611 228 190 163 115 93
93 82 78 77 76 74 69 62
46
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 776 3330 776
Size: 2554 1600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 873 1121 1204 1418 1720 2101 873
Size: 248 83 214 302 381 2926
Fragments arranged by size:
2926 381 302 248 214 83
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 684 2066 684
Size: 1382 2772
BsrFI r'CCGG_y
Cuts at: 2152 2333 2542 2152
Size: 181 209 3764
Fragments arranged by size:
3764 209 181
BssSI C'ACGA_G
Cuts at: 140 2425 2783 140
Size: 2285 358 1511
Fragments arranged by size:
2285 1511 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstYI r'GATC_y
Cuts at: 1 423 551 764 849 1179 1646 1669 2004
Size: 422 128 213 85 330 467 23 335
Cuts at: 2004 2250 3262 3348 3360 3706 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 422 346 335 330 246
213 128 86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 290 290
Size: 4154
BtgI C'CryG_G
Cuts at: 1145 1145
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 197 311 1397 197
Size: 114 1086 2954
Fragments arranged by size:
2954 1086 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 600 635 600
Size: 35 4119
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 621 974 1739 1913 2304 2331 3676 621
Size: 353 765 174 391 27 1345 1099
Fragments arranged by size:
1345 1099 765 391 353 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 118 755 1016 1037 2177 2387 118
Size: 637 261 21 1140 210 1885
Fragments arranged by size:
1885 1140 637 261 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 298 413 582 1166 298
Size: 115 169 584 3286
Fragments arranged by size:
3286 584 169 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 892 1979 2411 3158 3426 532
Size: 360 1087 432 747 268 1260
Fragments arranged by size:
1260 1087 747 432 360 268
EcoNI CCTnn'n_nnAGG
Cuts at: 239 239
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 1133 1293 1133
Size: 160 3994
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 558 2173 558
Size: 1615 2539
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 994 1026 1040 1072 1996 2026 2028
Size: 32 800 32 14 32 924 30 2
Cuts at: 2028 2058 162
Size: 30 2258
Fragments arranged by size:
2258 924 800 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 623 976 1915 623
Size: 353 939 2862
Fragments arranged by size:
2862 939 353
MslI CAynn'nnrTG
Cuts at: 781 952 2143 2270 781
Size: 171 1191 127 2665
Fragments arranged by size:
2665 1191 171 127
MspA1I CmG'CkG
Cuts at: 325 1265 1579 1939 2952 3197 325
Size: 940 314 360 1013 245 1282
Fragments arranged by size:
1282 1013 940 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NcoI C'CATG_G
Cuts at: 1145 1145
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PasI CC'CwG_GG
Cuts at: 174 174
Size: 4154
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PflMI CCAn_nnn'nTGG
Cuts at: 852 1146 852
Size: 294 3860
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PpuMI rG'GwC_Cy
Cuts at: 1133 1133
Size: 4154
PspXI vC'TCGA_Gb
Cuts at: 964 964
Size: 4154
PstI C_TGCA'G
Cuts at: 204 324 1201 1886 204
Size: 120 877 685 2472
Fragments arranged by size:
2472 877 685 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 325 1579 1939 325
Size: 1254 360 2540
Fragments arranged by size:
2540 1254 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 300 415 584 1168 300
Size: 115 169 584 3286
Fragments arranged by size:
3286 584 169 115
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 200 320 428 1197 1882 2875 3066 33
Size: 167 120 108 769 685 993 191 1121
Fragments arranged by size:
1121 993 769 685 191 167 120 108
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 181 964 1309 2224 2716 2978 181
Size: 783 345 915 492 262 1357
Fragments arranged by size:
1357 915 783 492 345 262
SnaBI TAC'GTA
Cuts at: 469 469
Size: 4154
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 363 1145 1288 1642 363
Size: 782 143 354 2875
Fragments arranged by size:
2875 782 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 318 712 859 1737 1870 2329 2538 318
Size: 394 147 878 133 459 209 1934
Fragments arranged by size:
1934 878 459 394 209 147 133
TatI w'GTAC_w
Cuts at: 530 3669 530
Size: 3139 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4122
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1114 1185 1114
Size: 71 4083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XhoI C'TCGA_G
Cuts at: 964 964
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XmnI ZraI