Restriction map for
pF1KB7151
(Circular) (Six-base) MAPSORT of: pF1KB7151.seq Check: 4329 from: 1 to: 4163
pF1KB7151 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 162 162
Size: 4163
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 1988 2420 3167 3435 720
Size: 1268 432 747 268 1448
Fragments arranged by size:
1448 1268 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 610 1468 3035 610
Size: 858 1567 1738
Fragments arranged by size:
1738 1567 858
ApaI G_GGCC'C
Cuts at: 554 1077 554
Size: 523 3640
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 117 403 1167 3516 3772 117
Size: 286 764 2349 256 508
Fragments arranged by size:
2349 764 508 286 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 223 1183 223
Size: 960 3203
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 590 623 590
Size: 33 4130
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 554 1077 1177 2207 554
Size: 523 100 1030 2510
Fragments arranged by size:
2510 1030 523 100
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 438 1005 438
Size: 567 3596
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 542 1683 542
Size: 1141 3022
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 554 972 1077 1788 1881 2937 554
Size: 418 105 711 93 1056 1780
Fragments arranged by size:
1780 1056 711 418 105 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1087 164
Size: 923 3240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 266 719 1352 1873 2551 2825 2972 3383 266
Size: 453 633 521 678 274 147 411 1046
Fragments arranged by size:
1046 678 633 521 453 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 562 562
Size: 4163
BseYI C'CCAG_C
Cuts at: 1588 2346 2923 3419 1588
Size: 758 577 496 2332
Fragments arranged by size:
2332 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 618
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 554 972 1077 1177 1788 1881 1955 2145 2207
Size: 418 105 100 611 93 74 190 62
Cuts at: 2207 2937 554
Size: 730 1780
Fragments arranged by size:
1780 730 611 418 190 105 100 93
74 62
BspEI T'CCGG_A
Cuts at: 266 3383 266
Size: 3117 1046
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 1213 1427 1729 2110 162
Size: 1051 214 302 381 2215
Fragments arranged by size:
2215 1051 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 461 577 2075 461
Size: 116 1498 2549
Fragments arranged by size:
2549 1498 116
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BsrGI T'GTAC_A
Cuts at: 932 932
Size: 4163
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 788 1188 1655 1678 2013 2259 3271 3357
Size: 787 400 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 400 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 311 365 107
Size: 204 54 3905
Fragments arranged by size:
3905 204 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 959 1406 959
Size: 447 3716
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 308 530 686 1748 1922 2313 2340 3685 308
Size: 222 156 1062 174 391 27 1345 786
Fragments arranged by size:
1345 1062 786 391 222 174 156 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 181 2186 2396 181
Size: 2005 210 1948
Fragments arranged by size:
2005 1948 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 720 1087 1988 2420 3167 3435 164
Size: 556 367 901 432 747 268 892
Fragments arranged by size:
901 892 747 556 432 367 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1081
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 228 551 831 1073 1074 1302 228
Size: 323 280 242 1 228 3089
Fragments arranged by size:
3089 323 280 242 228 1
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
EcoRV GAT'ATC
Cuts at: 186 186
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 819 2182 819
Size: 1363 2800
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 701 733 2005 2035 2037 2067 701
Size: 32 1272 30 2 30 2797
Fragments arranged by size:
2797 1272 32 30 30 2
HincII GTy'rAC
Cuts at: 701 1202 701
Size: 501 3662
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 532 1924 532
Size: 1392 2771
MslI CAynn'nnrTG
Cuts at: 595 2152 2279 595
Size: 1557 127 2479
Fragments arranged by size:
2479 1557 127
MspA1I CmG'CkG
Cuts at: 476 989 1274 1588 1948 2961 3206 476
Size: 513 285 314 360 1013 245 1433
Fragments arranged by size:
1433 1013 513 360 314 285 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 365 107
Size: 258 3905
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 933 1216 1716 2623 933
Size: 283 500 907 2473
Fragments arranged by size:
2473 907 500 283
PasI CC'CwG_GG
Cuts at: 554 554
Size: 4163
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PfoI T'CCnGG_A
Cuts at: 179 1031 179
Size: 852 3311
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 228 831 228
Size: 603 3560
PspOMI G'GGCC_C
Cuts at: 550 1073 550
Size: 523 3640
PstI C_TGCA'G
Cuts at: 1137 1210 1895 1137
Size: 73 685 3405
Fragments arranged by size:
3405 685 73
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 989 1588 1948 989
Size: 599 360 3204
Fragments arranged by size:
3204 599 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SexAI A'CCwGG_T
Cuts at: 609 609
Size: 4163
SfcI C'TryA_G
Cuts at: 33 602 1133 1206 1891 2884 3075 33
Size: 569 531 73 685 993 191 1121
Fragments arranged by size:
1121 993 685 569 531 191 73
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2725 2987 1318
Size: 915 492 262 2494
Fragments arranged by size:
2494 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 365 1297 1651 107
Size: 258 932 354 2619
Fragments arranged by size:
2619 932 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 546 759 1096 1746 1879 2338 2547 243
Size: 303 213 337 650 133 459 209 1859
Fragments arranged by size:
1859 650 459 337 303 213 209 133
TatI w'GTAC_w
Cuts at: 932 3678 932
Size: 2746 1417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI