Restriction map for
pF1KB7156
(Circular) (Six-base) MAPSORT of: pF1KB7156.seq Check: 8622 from: 1 to: 4190
pF1KB7156 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 559 559
Size: 4190
AccI GT'mk_AC
Cuts at: 365 1228 365
Size: 863 3327
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 304 404 2015 2447 3194 3462 149
Size: 155 100 1611 432 747 268 877
Fragments arranged by size:
1611 877 747 432 268 155 100
AflIII A'CryG_T
Cuts at: 466 1542 2646 3623 466
Size: 1076 1104 977 1033
Fragments arranged by size:
1104 1076 1033 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 312 669 788 1030 1194 3543 3799 312
Size: 357 119 242 164 2349 256 703
Fragments arranged by size:
2349 703 357 256 242 164 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 1204 2234 1204
Size: 1030 3160
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 292 292
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 734 1710 734
Size: 976 3214
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 1815 1908 2964 1815
Size: 93 1056 3041
Fragments arranged by size:
3041 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 721 1402 1483 1818 3962 721
Size: 681 81 335 2144 949
Fragments arranged by size:
2144 949 681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1033
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 467 922 1543 467
Size: 455 621 3114
Fragments arranged by size:
3114 621 455
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 690 1615 2373 2950 3446 690
Size: 925 758 577 496 1434
Fragments arranged by size:
1434 925 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 4190
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1114 1204 1982 2172 2964 1114
Size: 90 778 190 792 2340
Fragments arranged by size:
2340 792 778 190 90
Bsp1286I G_dGCh'C
Cuts at: 1114 1204 1815 1908 1982 2172 2234 2964 1114
Size: 90 611 93 74 190 62 730 2340
Fragments arranged by size:
2340 730 611 190 93 90 74 62
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 194 3366 194
Size: 3172 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 559 1240 1454 1756 2137 559
Size: 681 214 302 381 2612
Fragments arranged by size:
2612 681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
ClaI AT'CG_AT
Cuts at: 483 483
Size: 4190
DraI TTT'AAA
Cuts at: 1035 1189 1035
Size: 154 4036
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 293 581 953 1775 1949 2340 2367 3712 293
Size: 288 372 822 174 391 27 1345 771
Fragments arranged by size:
1345 822 771 391 372 288 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 126 2213 2423 126
Size: 2087 210 1893
Fragments arranged by size:
2087 1893 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 304 404 1033 2015 2447 3194 3462 149
Size: 155 100 629 982 432 747 268 877
Fragments arranged by size:
982 877 747 629 432 268 155 100
EcoNI CCTnn'n_nnAGG
Cuts at: 775 775
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 768 1329 768
Size: 561 3629
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1031 1063 2032 2062 2064 2094 1031
Size: 32 969 30 2 30 3127
Fragments arranged by size:
3127 969 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MfeI C'AATT_G
Cuts at: 839 990 839
Size: 151 4039
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 295 583 1951 295
Size: 288 1368 2534
Fragments arranged by size:
2534 1368 288
MslI CAynn'nnrTG
Cuts at: 223 373 608 955 2179 2306 223
Size: 150 235 347 1224 127 2107
Fragments arranged by size:
2107 1224 347 235 150 127
MspA1I CmG'CkG
Cuts at: 386 1301 1615 1975 2988 3233 386
Size: 915 314 360 1013 245 1343
Fragments arranged by size:
1343 1013 915 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PfoI T'CCnGG_A
Cuts at: 862 862
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 467 467
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PstI C_TGCA'G
Cuts at: 1237 1922 1237
Size: 685 3505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 927 1233 1918 2911 3102 33
Size: 894 306 685 993 191 1121
Fragments arranged by size:
1121 993 894 685 306 191
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
SspI AAT'ATT
Cuts at: 236 636 881 236
Size: 400 245 3545
Fragments arranged by size:
3545 400 245
StyI C'CwwG_G
Cuts at: 1324 1678 1324
Size: 354 3836
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 518 1773 1906 2365 2574 518
Size: 1255 133 459 209 2134
Fragments arranged by size:
2134 1255 459 209 133
TatI w'GTAC_w
Cuts at: 3705 3705
Size: 4190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 1685 708
Size: 977 3213
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI