Restriction map for
pF1KB7166
(Circular) (Six-base) MAPSORT of: pF1KB7166.seq Check: 2136 from: 1 to: 4301
pF1KB7166 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2558 3305 3573 2126
Size: 432 747 268 2854
Fragments arranged by size:
2854 747 432 268
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 651 1606 3173 651
Size: 955 1567 1779
Fragments arranged by size:
1779 1567 955
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 256 1305 3654 3910 256
Size: 1049 2349 256 647
Fragments arranged by size:
2349 1049 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1202 1321 1202
Size: 119 4182
BamHI G'GATC_C
Cuts at: 516 918 1326 516
Size: 402 408 3491
Fragments arranged by size:
3491 408 402
BanI G'GyrC_C
Cuts at: 1317 1731 1979 2014 1317
Size: 414 248 35 3604
Fragments arranged by size:
3604 414 248 35
BanII G_rGCy'C
Cuts at: 486 626 1315 2345 486
Size: 140 689 1030 2442
Fragments arranged by size:
2442 1030 689 140
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 189 1285 189
Size: 1096 3205
BciVI GTATCCnnnnn_n'
Cuts at: 539 2199 2960 4301 539
Size: 1660 761 1341 539
Fragments arranged by size:
1660 1341 761 539
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 173 1816 3853 173
Size: 1643 2037 621
Fragments arranged by size:
2037 1643 621
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 1926 2019 3075 1926
Size: 93 1056 3152
Fragments arranged by size:
3152 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1132
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 587 1142 587
Size: 555 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1477 2356 2848 3146 934
Size: 543 879 492 298 2089
Fragments arranged by size:
2089 879 543 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 1654 1654
Size: 4301
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 200 1490 2011 2689 2963 3110 3521 200
Size: 1290 521 678 274 147 411 980
Fragments arranged by size:
1290 980 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 239 514 1105 154
Size: 85 275 591 3350
Fragments arranged by size:
3350 591 275 85
BseYI C'CCAG_C
Cuts at: 1101 1234 1726 2484 3061 3557 1101
Size: 133 492 758 577 496 1845
Fragments arranged by size:
1845 758 577 496 492 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 753
Size: 4301
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 419 1315 2093 2283 3075 419
Size: 896 778 190 792 1645
Fragments arranged by size:
1645 896 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 419 486 626 1315 1926 2019 2093 2283 2345
Size: 67 140 689 611 93 74 190 62
Cuts at: 2345 3075 419
Size: 730 1645
Fragments arranged by size:
1645 730 689 611 190 140 93 74
67 62
BspEI T'CCGG_A
Cuts at: 200 3521 200
Size: 3321 980
BspHI T'CATG_A
Cuts at: 626 3477 626
Size: 2851 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 467 980 2213 467
Size: 513 1233 2555
Fragments arranged by size:
2555 1233 513
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 4301
BstYI r'GATC_y
Cuts at: 1 173 516 918 1155 1326 1793 1816 2151
Size: 172 343 402 237 171 467 23 335
Cuts at: 2151 2397 3409 3495 3507 3853 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 402 346 343 335 246
237 172 171 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1544 1250
Size: 294 4007
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 746 2007 2865 746
Size: 1261 858 2182
Fragments arranged by size:
2182 1261 858
EaeI y'GGCC_r
Cuts at: 597 1886 2060 2451 2478 3823 597
Size: 1289 174 391 27 1345 1075
Fragments arranged by size:
1345 1289 1075 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 506 2324 2534 506
Size: 1818 210 2273
Fragments arranged by size:
2273 1818 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2126 2558 3305 3573 1132
Size: 994 432 747 268 1860
Fragments arranged by size:
1860 994 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 136 136
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 1440 1440
Size: 4301
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 813 2320 813
Size: 1507 2794
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 2143 2173 2175 2205 218
Size: 32 1893 30 2 30 2314
Fragments arranged by size:
2314 1893 32 30 30 2
HincII GTy'rAC
Cuts at: 1340 1340
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 94 1265 2972 3156 94
Size: 1171 1707 184 1239
Fragments arranged by size:
1707 1239 1171 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 163 355 2290 2417 163
Size: 192 1935 127 2047
Fragments arranged by size:
2047 1935 192 127
MspA1I CmG'CkG
Cuts at: 217 1105 1125 1412 1726 2086 3099 3344 217
Size: 888 20 287 314 360 1013 245 1174
Fragments arranged by size:
1174 1013 888 360 314 287 245 20
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 1354 1854 2761 1354
Size: 500 907 2894
Fragments arranged by size:
2894 907 500
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PshAI GACnn'nnGTC
Cuts at: 1285 1285
Size: 4301
PsiI TTA'TAA
Cuts at: 932 932
Size: 4301
PstI C_TGCA'G
Cuts at: 647 1124 1348 2033 647
Size: 477 224 685 2915
Fragments arranged by size:
2915 685 477 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 217 1105 1726 2086 217
Size: 888 621 360 2432
Fragments arranged by size:
2432 888 621 360
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 218 643 1120 1173 1344 2029 3022 3213
Size: 185 425 477 53 171 685 993 191
Cuts at: 3213 33
Size: 1121
Fragments arranged by size:
1121 993 685 477 425 191 185 171
53
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1204 1323 1204
Size: 119 4182
SmlI C'TyrA_G
Cuts at: 913 1456 2371 2863 3125 913
Size: 543 915 492 262 2089
Fragments arranged by size:
2089 915 543 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StuI AGG'CCT
Cuts at: 827 1171 827
Size: 344 3957
StyI C'CwwG_G
Cuts at: 1435 1789 1435
Size: 354 3947
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 327 1884 2017 2476 2685 327
Size: 1557 133 459 209 1943
Fragments arranged by size:
1943 1557 459 209 133
TatI w'GTAC_w
Cuts at: 1007 3816 1007
Size: 2809 1492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 3818 3850 139
Size: 32 3647 32 590
Fragments arranged by size:
3647 590 32 32
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 775 1332 775
Size: 557 3744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1202 1321 1202
Size: 119 4182
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI