Restriction map for
pF1KB7170
(Circular) (Six-base) MAPSORT of: pF1KB7170.seq Check: 2591 from: 1 to: 4430
pF1KB7170 4430 bp
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 497 497
Size: 4430
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2687 3434 3702 2255
Size: 432 747 268 2983
Fragments arranged by size:
2983 747 432 268
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AleI CACnn'nnGTG
Cuts at: 172 172
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 804 1155 1179 1198 1735 3302 804
Size: 351 24 19 537 1567 1932
Fragments arranged by size:
1932 1567 537 351 24 19
ApaI G_GGCC'C
Cuts at: 688 1259 688
Size: 571 3859
ApaLI G'TGCA_C
Cuts at: 403 639 3200 403
Size: 236 2561 1633
Fragments arranged by size:
2561 1633 236
ApoI r'AATT_y
Cuts at: 1434 3783 4039 1434
Size: 2349 256 1825
Fragments arranged by size:
2349 1825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 459 479 681 1136 1250 1450 459
Size: 20 202 455 114 200 3439
Fragments arranged by size:
3439 455 202 200 114 20
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 437 598 1270 1336 1446 1860 2108 2143 437
Size: 161 672 66 110 414 248 35 2724
Fragments arranged by size:
2724 672 414 248 161 110 66 35
BanII G_rGCy'C
Cuts at: 521 545 688 1259 1444 2474 521
Size: 24 143 571 185 1030 2477
Fragments arranged by size:
2477 1030 571 185 143 24
BbeI G_GCGC'C
Cuts at: 441 602 1274 1864 2112 441
Size: 161 672 590 248 2759
Fragments arranged by size:
2759 672 590 248 161
BbsI GAAGACnn'nnnn_
Cuts at: 198 342 897 1414 198
Size: 144 555 517 3214
Fragments arranged by size:
3214 555 517 144
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglI GCCn_nnn'nGGC
Cuts at: 1013 1174 1334 1013
Size: 161 160 4109
Fragments arranged by size:
4109 161 160
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 407 643 688 1259 1312 2055 2148 3204 407
Size: 236 45 571 53 743 93 1056 1633
Fragments arranged by size:
1633 1056 743 571 236 93 53 45
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 166 144
Size: 22 4408
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 4430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2485 2977 3275 1606
Size: 879 492 298 2761
Fragments arranged by size:
2761 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4134 4134
Size: 4430
BsaAI yAC'GTr
Cuts at: 336 747 864 924 1783 336
Size: 411 117 60 859 2983
Fragments arranged by size:
2983 859 411 117 60
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 314 438 599 1271 1861 2109 2811 4307 314
Size: 124 161 672 590 248 702 1496 437
Fragments arranged by size:
1496 702 672 590 437 248 161 124
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 622 652 148
Size: 30 444 30 3926
Fragments arranged by size:
3926 444 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 550 553 577 580 583 586 589 592
Size: 387 3 24 3 3 3 3 3
Cuts at: 592 631 646 163
Size: 39 15 3947
Fragments arranged by size:
3947 387 39 24 15 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 549 752 1198 1855 2613 3190 3686 549
Size: 203 446 657 758 577 496 1293
Fragments arranged by size:
1293 758 657 577 496 446 203
BsiEI CG_ry'CG
Cuts at: 104 653 692 829 1008 1079 1127 1212 1972
Size: 549 39 137 179 71 48 85 760
Cuts at: 1972 2018 3226 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 760 549 179 137 85 71
48 46 39
BsiHKAI G_wGCw'C
Cuts at: 407 643 1444 2222 2412 3204 407
Size: 236 801 778 190 792 1633
Fragments arranged by size:
1633 801 792 778 236 190
BsmBI CGTCTCn'nnnn_
Cuts at: 574 574
Size: 4430
Bsp1286I G_dGCh'C
Cuts at: 407 521 545 643 688 1259 1312 1444 2055
Size: 114 24 98 45 571 53 132 611
Cuts at: 2055 2148 2222 2412 2474 3204 407
Size: 93 74 190 62 730 1633
Fragments arranged by size:
1633 730 611 571 190 132 114 98
93 74 62 53 45 24
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 497 812 1480 1694 1996 2377 497
Size: 315 668 214 302 381 2550
Fragments arranged by size:
2550 668 381 315 302 214
BsrBI CCG'CTC
Cuts at: 50 435 1347 2722 2776 4260 4425 50
Size: 385 912 1375 54 1484 165 55
Fragments arranged by size:
1484 1375 912 385 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 505 601 1328 2428 2609 2818 505
Size: 96 727 1100 181 209 2117
Fragments arranged by size:
2117 1100 727 209 181 96
BssHII G'CGCG_C
Cuts at: 473 986 1131 473
Size: 513 145 3772
Fragments arranged by size:
3772 513 145
BssSI C'ACGA_G
Cuts at: 1342 2701 3059 1342
Size: 1359 358 2713
Fragments arranged by size:
2713 1359 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1179 1807 1179
Size: 628 3802
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 174 174
Size: 4430
BstYI r'GATC_y
Cuts at: 1 152 1455 1922 1945 2280 2526 3538 3624
Size: 151 1303 467 23 335 246 1012 86
Cuts at: 3624 3636 3982 1
Size: 12 346 449
Fragments arranged by size:
1303 1012 467 449 346 335 246 151
86 23 12
BtgI C'CryG_G
Cuts at: 1011 1045 1011
Size: 34 4396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4086
Fragments arranged by size:
4086 181 163
BtsI GCAGTG_nn'
Cuts at: 1299 1392 1673 1299
Size: 93 281 4056
Fragments arranged by size:
4056 281 93
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DraIII CAC_nnn'GTG
Cuts at: 867 1418 867
Size: 551 3879
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2994 2136
Size: 858 3572
EaeI y'GGCC_r
Cuts at: 761 1005 1076 1124 1348 2015 2189 2580 2607
Size: 244 71 48 224 667 174 391 27
Cuts at: 2607 3952 761
Size: 1345 1239
Fragments arranged by size:
1345 1239 667 391 244 224 174 71
48 27
EagI C'GGCC_G
Cuts at: 1005 1076 1124 2015 1005
Size: 71 48 891 3420
Fragments arranged by size:
3420 891 71 48
EarI CTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 723 1017 2638 2948 3094 4240 439
Size: 284 294 1621 310 146 1146 629
Fragments arranged by size:
1621 1146 629 310 294 284 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 166 2255 2687 3434 3702 144
Size: 22 2089 432 747 268 872
Fragments arranged by size:
2089 872 747 432 268 22
EcoNI CCTnn'n_nnAGG
Cuts at: 532 559 532
Size: 27 4403
EcoO109I rG'GnC_Cy
Cuts at: 677 729 1255 1256 1569 677
Size: 52 526 1 313 3538
Fragments arranged by size:
3538 526 313 52 1
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 921 2449 921
Size: 1528 2902
FseI GG_CCGG'CC
Cuts at: 1332 1332
Size: 4430
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 441 602 659 1180 1274 1864 2112 3134 4113
Size: 161 57 521 94 590 248 1022 979
Cuts at: 4113 441
Size: 758
Fragments arranged by size:
1022 979 758 590 521 248 161 94
57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2272 2302 2304 2334 2272
Size: 30 2 30 4368
Fragments arranged by size:
4368 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 437 598 1270 1860 2108 437
Size: 161 672 590 248 2759
Fragments arranged by size:
2759 672 590 248 161
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3285 3101
Size: 184 4246
MscI TGG'CCA
Cuts at: 763 2191 763
Size: 1428 3002
MslI CAynn'nnrTG
Cuts at: 172 2419 2546 172
Size: 2247 127 2056
Fragments arranged by size:
2247 2056 127
MspA1I CmG'CkG
Cuts at: 723 756 932 1013 1047 1081 1195 1541 1855
Size: 33 176 81 34 34 114 346 314
Cuts at: 1855 2215 3228 3473 723
Size: 360 1013 245 1680
Fragments arranged by size:
1680 1013 360 346 314 245 176 114
81 34 34 33
NaeI GCC'GGC
Cuts at: 507 603 1330 2611 507
Size: 96 727 1281 2326
Fragments arranged by size:
2326 1281 727 96
NarI GG'CG_CC
Cuts at: 438 599 1271 1861 2109 438
Size: 161 672 590 248 2759
Fragments arranged by size:
2759 672 590 248 161
NgoMIV G'CCGG_C
Cuts at: 505 601 1328 2609 505
Size: 96 727 1281 2326
Fragments arranged by size:
2326 1281 727 96
NotI GC'GGCC_GC
Cuts at: 1005 1076 1005
Size: 71 4359
NspI r_CATG'y
Cuts at: 1483 1983 2890 1483
Size: 500 907 3023
Fragments arranged by size:
3023 907 500
PasI CC'CwG_GG
Cuts at: 1364 1364
Size: 4430
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PflMI CCAn_nnn'nTGG
Cuts at: 360 759 1155 1418 360
Size: 399 396 263 3372
Fragments arranged by size:
3372 399 396 263
PfoI T'CCnGG_A
Cuts at: 1282 1282
Size: 4430
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PmlI CAC'GTG
Cuts at: 336 864 336
Size: 528 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PpuMI rG'GwC_Cy
Cuts at: 677 677
Size: 4430
PspOMI G'GGCC_C
Cuts at: 684 1255 684
Size: 571 3859
PstI C_TGCA'G
Cuts at: 844 1175 1477 2162 844
Size: 331 302 685 3112
Fragments arranged by size:
3112 685 331 302
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 756 932 1855 2215 756
Size: 176 923 360 2971
Fragments arranged by size:
2971 923 360 176
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SacII CC_GC'GG
Cuts at: 1014 1048 1014
Size: 34 4396
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SanDI GG'GwC_CC
Cuts at: 677 677
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1175 1477 1175
Size: 302 4128
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 840 1104 1171 1473 2158 3151 3342 33
Size: 807 264 67 302 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 302 264 191 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 1013 1013
Size: 4430
SfoI GGC'GCC
Cuts at: 439 600 1272 1862 2110 439
Size: 161 672 590 248 2759
Fragments arranged by size:
2759 672 590 248 161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SgrAI Cr'CCGG_yG
Cuts at: 601 601
Size: 4430
SmaI CCC'GGG
Cuts at: 461 683 1252 1452 461
Size: 222 569 200 3439
Fragments arranged by size:
3439 569 222 200
SmlI C'TyrA_G
Cuts at: 1585 2500 2992 3254 1585
Size: 915 492 262 2761
Fragments arranged by size:
2761 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
StyI C'CwwG_G
Cuts at: 1564 1918 1564
Size: 354 4076
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 369 1354 2013 2146 2605 2814 369
Size: 985 659 133 459 209 1985
Fragments arranged by size:
1985 985 659 459 209 133
TatI w'GTAC_w
Cuts at: 3945 3945
Size: 4430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 622 654 1278 1310 3947 3979 148
Size: 32 442 32 624 32 2637 32 599
Fragments arranged by size:
2637 624 599 442 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 301 2227 301
Size: 1926 2504
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 1925 552
Size: 1373 3057
XmaI C'CCGG_G
Cuts at: 459 681 1250 1450 459
Size: 222 569 200 3439
Fragments arranged by size:
3439 569 222 200
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PshAI PsiI PspXI PsrI SexAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI