Restriction map for
pF1KB7171
(Circular) (Six-base) MAPSORT of: pF1KB7171.seq Check: 8810 from: 1 to: 4559
pF1KB7171 4559 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1464 2384 2816 3563 3831 277
Size: 1187 920 432 747 268 1005
Fragments arranged by size:
1187 1005 920 747 432 268
AfeI AGC'GCT
Cuts at: 1005 1005
Size: 4559
AflIII A'CryG_T
Cuts at: 422 1495 1911 3015 3992 422
Size: 1073 416 1104 977 989
Fragments arranged by size:
1104 1073 989 977 416
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 1864 3431 1864
Size: 1567 2992
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 631 1463 1563 3912 4168 631
Size: 832 100 2349 256 1022
Fragments arranged by size:
2349 1022 832 256 100
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
AvrII C'CTAG_G
Cuts at: 835 835
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 170 1493 1573 2603 170
Size: 1323 80 1030 2126
Fragments arranged by size:
2126 1323 1030 80
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BbsI GAAGACnn'nnnn_
Cuts at: 429 783 1179 429
Size: 354 396 3809
Fragments arranged by size:
3809 396 354
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 765 2079 765
Size: 1314 3245
BfrBI ATG'CAT
Cuts at: 1367 1367
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 2184 2277 3333 2184
Size: 93 1056 3410
Fragments arranged by size:
3410 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 166 223 911 1771 1852 2187 4331 166
Size: 57 688 860 81 335 2144 394
Fragments arranged by size:
2144 860 688 394 335 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 627 740 1489 343
Size: 284 113 749 3413
Fragments arranged by size:
3413 749 284 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 2614 3106 3404 1735
Size: 879 492 298 2890
Fragments arranged by size:
2890 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 643 698 906 4263 121
Size: 522 55 208 3357 417
Fragments arranged by size:
3357 522 417 208 55
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 1085 2073 4110 6
Size: 1079 988 2037 455
Fragments arranged by size:
2037 1079 988 455
BsaHI Gr'CG_yC
Cuts at: 112 1990 2238 2940 4436 112
Size: 1878 248 702 1496 235
Fragments arranged by size:
1878 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 1501 304
Size: 1197 3362
BseYI C'CCAG_C
Cuts at: 1984 2742 3319 3815 1984
Size: 758 577 496 2728
Fragments arranged by size:
2728 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1493 1573 2351 2541 3333 1493
Size: 80 778 190 792 2719
Fragments arranged by size:
2719 792 778 190 80
BsmI GAATG_Cn'
Cuts at: 569 1199 569
Size: 630 3929
Bsp1286I G_dGCh'C
Cuts at: 170 1493 1573 2184 2277 2351 2541 2603 3333
Size: 1323 80 611 93 74 190 62 730
Cuts at: 3333 170
Size: 1396
Fragments arranged by size:
1396 1323 730 611 190 93 80 74
62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 1160 3735 1160
Size: 2575 1984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 897 1609 1823 2125 2506 371
Size: 526 712 214 302 381 2424
Fragments arranged by size:
2424 712 526 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 234 516 1306 1367 2471 234
Size: 282 790 61 1104 2322
Fragments arranged by size:
2322 1104 790 282 61
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 1105 2830 3188 1105
Size: 1725 358 2476
Fragments arranged by size:
2476 1725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 459 954 1294 459
Size: 495 340 3724
Fragments arranged by size:
3724 495 340
BstYI r'GATC_y
Cuts at: 1 675 741 1584 2051 2074 2409 2655 3667
Size: 674 66 843 467 23 335 246 1012
Cuts at: 3667 3753 3765 4111 1
Size: 86 12 346 449
Fragments arranged by size:
1012 843 674 467 449 346 335 246
86 66 23 12
BtgI C'CryG_G
Cuts at: 107 157 185 1244 107
Size: 50 28 1059 3422
Fragments arranged by size:
3422 1059 50 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 490 2509 2690 2853 490
Size: 2019 181 163 2196
Fragments arranged by size:
2196 2019 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 885 989 1558 885
Size: 104 569 3886
Fragments arranged by size:
3886 569 104
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 1241 2144 2318 2709 2736 4081 1241
Size: 903 174 391 27 1345 1719
Fragments arranged by size:
1719 1345 903 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 322 2767 3077 3223 4369 322
Size: 2445 310 146 1146 512
Fragments arranged by size:
2445 1146 512 310 146
EcoICRI GAG'CTC
Cuts at: 1491 1571 1491
Size: 80 4479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 343 627 740 1464 1489 2384 2816 3563
Size: 66 284 113 724 25 895 432 747
Cuts at: 3563 3831 277
Size: 268 1005
Fragments arranged by size:
1005 895 747 724 432 284 268 113
66 25
EcoNI CCTnn'n_nnAGG
Cuts at: 378 378
Size: 4559
EcoO109I rG'GnC_Cy
Cuts at: 1698 1698
Size: 4559
EcoRI G'AATT_C
Cuts at: 1463 1563 1463
Size: 100 4459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1007 1993 2241 3263 4242 1007
Size: 986 248 1022 979 1324
Fragments arranged by size:
1324 1022 986 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 733 765 886 918 2401 2431 2433
Size: 32 568 32 121 32 1483 30 2
Cuts at: 2433 2463 133
Size: 30 2229
Fragments arranged by size:
2229 1483 568 121 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 854 1598 854
Size: 744 3815
HpaI GTT'AAC
Cuts at: 854 854
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 819 3230 3414 819
Size: 2411 184 1964
Fragments arranged by size:
2411 1964 184
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 156 509 574 2548 2675 156
Size: 353 65 1974 127 2040
Fragments arranged by size:
2040 1974 353 127 65
MspA1I CmG'CkG
Cuts at: 267 1670 1984 2344 3357 3602 267
Size: 1403 314 360 1013 245 1224
Fragments arranged by size:
1403 1224 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 107 157 185 107
Size: 50 28 4481
Fragments arranged by size:
4481 50 28
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NsiI A_TGCA'T
Cuts at: 1369 1369
Size: 4559
NspI r_CATG'y
Cuts at: 426 1612 2112 3019 426
Size: 1186 500 907 1966
Fragments arranged by size:
1966 1186 907 500
PciI A'CATG_T
Cuts at: 422 3015 422
Size: 2593 1966
PflMI CCAn_nnn'nTGG
Cuts at: 229 229
Size: 4559
PfoI T'CCnGG_A
Cuts at: 1049 1049
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PstI C_TGCA'G
Cuts at: 825 1606 2291 825
Size: 781 685 3093
Fragments arranged by size:
3093 781 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1493 1573 1493
Size: 80 4479
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 602 821 1602 2287 3280 3471 33
Size: 569 219 781 685 993 191 1121
Fragments arranged by size:
1121 993 781 685 569 219 191
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1714 2629 3121 3383 1714
Size: 915 492 262 2890
Fragments arranged by size:
2890 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StyI C'CwwG_G
Cuts at: 107 157 185 302 772 835 1331 1693 2047
Size: 50 28 117 470 63 496 362 354
Cuts at: 2047 107
Size: 2619
Fragments arranged by size:
2619 496 470 362 354 117 63 50
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 537 1519 2142 2275 2734 2943 234
Size: 303 982 623 133 459 209 1850
Fragments arranged by size:
1850 982 623 459 303 209 133
TatI w'GTAC_w
Cuts at: 1543 4074 1543
Size: 2531 2028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 896 928 1031 1063 1494 1526 4076 4108 896
Size: 32 103 32 431 32 2550 32 1347
Fragments arranged by size:
2550 1347 431 103 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 619 2054 619
Size: 1435 3124
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI