Restriction map for
pF1KB7176
(Circular) (Six-base) MAPSORT of: pF1KB7176.seq Check: 8051 from: 1 to: 4208
pF1KB7176 4208 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 437 1246 437
Size: 809 3399
Acc65I G'GTAC_C
Cuts at: 188 1224 188
Size: 1036 3172
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 300 591 2033 2465 3212 3480 289
Size: 11 291 1442 432 747 268 1017
Fragments arranged by size:
1442 1017 747 432 291 268 11
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AleI CACnn'nnGTG
Cuts at: 787 787
Size: 4208
AlwNI CAG_nnn'CTG
Cuts at: 685 889 1513 3080 685
Size: 204 624 1567 1813
Fragments arranged by size:
1813 1567 624 204
ApaI G_GGCC'C
Cuts at: 116 140 544 827 1067 116
Size: 24 404 283 240 3257
Fragments arranged by size:
3257 404 283 240 24
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 1212 3561 3817 1212
Size: 2349 256 1603
Fragments arranged by size:
2349 1603 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 1058 1151 1228 1058
Size: 93 77 4038
Fragments arranged by size:
4038 93 77
AvrII C'CTAG_G
Cuts at: 847 847
Size: 4208
BamHI G'GATC_C
Cuts at: 1233 1233
Size: 4208
BanI G'GyrC_C
Cuts at: 188 387 645 717 877 987 1107 1224 1638
Size: 199 258 72 160 110 120 117 414
Cuts at: 1638 1886 1921 188
Size: 248 35 2475
Fragments arranged by size:
2475 414 258 248 199 160 120 117
110 72 35
BanII G_rGCy'C
Cuts at: 116 140 544 827 1067 1222 2252 116
Size: 24 404 283 240 155 1030 2072
Fragments arranged by size:
2072 1030 404 283 240 155 24
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbsI GAAGACnn'nnnn_
Cuts at: 329 329
Size: 4208
BbvCI CC'TCA_GC
Cuts at: 458 458
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 1728 1728
Size: 4208
BglI GCCn_nnn'nGGC
Cuts at: 570 756 1071 570
Size: 186 315 3707
Fragments arranged by size:
3707 315 186
BglII A'GATC_T
Cuts at: 1723 3760 1723
Size: 2037 2171
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 116 140 392 544 648 722 827 880 1067
Size: 24 252 152 104 74 105 53 187
Cuts at: 1067 1833 1926 2982 116
Size: 766 93 1056 1342
Fragments arranged by size:
1342 1056 766 252 187 152 105 104
93 74 53 24
BmgBI CAC'GTC
Cuts at: 441 441
Size: 4208
BmrI ACTGGGnnnn_n'
Cuts at: 572 590 673 1005 1047 1420 1501 1836 3980
Size: 18 83 332 42 373 81 335 2144
Cuts at: 3980 572
Size: 800
Fragments arranged by size:
2144 800 373 335 332 83 81 42
18
BmtI G_CTAG'C
Cuts at: 664 664
Size: 4208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 174 2575 174
Size: 2401 1807
Bpu10I CC'TnA_GC
Cuts at: 458 458
Size: 4208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2755 3053 1384
Size: 879 492 298 2539
Fragments arranged by size:
2539 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 4208
BsaAI yAC'GTr
Cuts at: 549 1561 549
Size: 1012 3196
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 1091 1639 1887 2589 4085 1091
Size: 548 248 702 1496 1214
Fragments arranged by size:
1496 1214 702 548 248
BsaWI w'CCGG_w
Cuts at: 1397 1918 2596 2870 3017 3428 1397
Size: 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 904 934 349
Size: 30 525 30 3623
Fragments arranged by size:
3623 525 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 724 189
Size: 535 3673
BseYI C'CCAG_C
Cuts at: 161 453 1633 2391 2968 3464 161
Size: 292 1180 758 577 496 905
Fragments arranged by size:
1180 905 758 577 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 826 1215 538
Size: 288 389 3531
Fragments arranged by size:
3531 389 288
BsiEI CG_ry'CG
Cuts at: 104 437 561 574 1750 1796 3004 104
Size: 333 124 13 1176 46 1208 1308
Fragments arranged by size:
1308 1208 1176 333 124 46 13
BsiHKAI G_wGCw'C
Cuts at: 1222 2000 2190 2982 1222
Size: 778 190 792 2448
Fragments arranged by size:
2448 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 116 140 392 544 648 722 827 880 1067
Size: 24 252 152 104 74 105 53 187
Cuts at: 1067 1222 1833 1926 2000 2190 2252 2982 116
Size: 155 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 252 190 187 155 152
105 104 93 74 74 62 53 24
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 1258 1472 1774 2155 200
Size: 1058 214 302 381 2253
Fragments arranged by size:
2253 1058 381 302 214
BsrBI CCG'CTC
Cuts at: 50 253 2500 2554 4038 4203 50
Size: 203 2247 54 1484 165 55
Fragments arranged by size:
2247 1484 203 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2120 2120
Size: 4208
BsrFI r'CCGG_y
Cuts at: 491 560 569 2206 2387 2596 491
Size: 69 9 1637 181 209 2103
Fragments arranged by size:
2103 1637 209 181 69 9
BssSI C'ACGA_G
Cuts at: 783 2479 2837 783
Size: 1696 358 2154
Fragments arranged by size:
2154 1696 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1585 1585
Size: 4208
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 537 789 537
Size: 252 3956
BstYI r'GATC_y
Cuts at: 1 1233 1700 1723 2058 2304 3316 3402 3414
Size: 1232 467 23 335 246 1012 86 12
Cuts at: 3414 3760 1
Size: 346 449
Fragments arranged by size:
1232 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 1451 1451
Size: 4208
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DraIII CAC_nnn'GTG
Cuts at: 598 598
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1138 1914 2772 1138
Size: 776 858 2574
Fragments arranged by size:
2574 858 776
EaeI y'GGCC_r
Cuts at: 224 558 562 571 652 1793 1967 2358 2385
Size: 334 4 9 81 1141 174 391 27
Cuts at: 2385 3730 224
Size: 1345 702
Fragments arranged by size:
1345 1141 702 391 334 174 81 27
9 4
EagI C'GGCC_G
Cuts at: 558 571 1793 558
Size: 13 1222 2973
Fragments arranged by size:
2973 1222 13
EarI CTCTTCn'nnn_
Cuts at: 178 744 1102 2231 2441 178
Size: 566 358 1129 210 1945
Fragments arranged by size:
1945 1129 566 358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 300 591 2033 2465 3212 3480 289
Size: 11 291 1442 432 747 268 1017
Fragments arranged by size:
1442 1017 747 432 291 268 11
EcoNI CCTnn'n_nnAGG
Cuts at: 315 1172 315
Size: 857 3351
EcoO109I rG'GnC_Cy
Cuts at: 112 113 136 137 396 534 541 824 851
Size: 1 23 1 259 138 7 283 27
Cuts at: 851 917 1063 1064 1155 1347 112
Size: 66 146 1 91 192 2973
Fragments arranged by size:
2973 283 259 192 146 138 91 66
27 23 7 1 1 1
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 2227 441
Size: 1786 2422
FseI GG_CCGG'CC
Cuts at: 564 564
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 466 498 1642 1890 2912 3891 466
Size: 32 1144 248 1022 979 783
Fragments arranged by size:
1144 1022 979 783 248 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 273 305 2050 2080 2082 2112 136
Size: 32 105 32 1745 30 2 30 2232
Fragments arranged by size:
2232 1745 105 32 32 30 30 2
HincII GTy'rAC
Cuts at: 438 1022 1247 438
Size: 584 225 3399
Fragments arranged by size:
3399 584 225
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 192 1228 192
Size: 1036 3172
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 392 2879 3063 392
Size: 2487 184 1537
Fragments arranged by size:
2487 1537 184
MscI TGG'CCA
Cuts at: 226 1969 226
Size: 1743 2465
MslI CAynn'nnrTG
Cuts at: 787 2197 2324 787
Size: 1410 127 2671
Fragments arranged by size:
2671 1410 127
MspA1I CmG'CkG
Cuts at: 122 457 905 936 1319 1633 1993 3006 3251
Size: 335 448 31 383 314 360 1013 245
Cuts at: 3251 122
Size: 1079
Fragments arranged by size:
1079 1013 448 383 360 335 314 245
31
NaeI GCC'GGC
Cuts at: 493 562 2389 493
Size: 69 1827 2312
Fragments arranged by size:
2312 1827 69
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NcoI C'CATG_G
Cuts at: 107 107
Size: 4208
NgoMIV G'CCGG_C
Cuts at: 491 560 2387 491
Size: 69 1827 2312
Fragments arranged by size:
2312 1827 69
NheI G'CTAG_C
Cuts at: 660 660
Size: 4208
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PasI CC'CwG_GG
Cuts at: 1069 1069
Size: 4208
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PpuMI rG'GwC_Cy
Cuts at: 396 534 1155 396
Size: 138 621 3449
Fragments arranged by size:
3449 621 138
PspOMI G'GGCC_C
Cuts at: 112 136 540 823 1063 112
Size: 24 404 283 240 3257
Fragments arranged by size:
3257 404 283 240 24
PstI C_TGCA'G
Cuts at: 121 476 690 875 1255 1940 121
Size: 355 214 185 380 685 2389
Fragments arranged by size:
2389 685 380 355 214 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 457 936 1633 1993 457
Size: 479 697 360 2672
Fragments arranged by size:
2672 697 479 360
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 436 1245 436
Size: 809 3399
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 690 1255 690
Size: 565 3643
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SfcI C'TryA_G
Cuts at: 33 117 472 686 871 1251 1936 2929 3120
Size: 84 355 214 185 380 685 993 191
Cuts at: 3120 33
Size: 1121
Fragments arranged by size:
1121 993 685 380 355 214 191 185
84
SfiI GGCCn_nnn'nGGCC
Cuts at: 570 756 570
Size: 186 4022
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4208
SmlI C'TyrA_G
Cuts at: 1363 2278 2770 3032 1363
Size: 915 492 262 2539
Fragments arranged by size:
2539 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StuI AGG'CCT
Cuts at: 750 750
Size: 4208
StyI C'CwwG_G
Cuts at: 107 236 392 416 530 847 983 1101 1342
Size: 129 156 24 114 317 136 118 241
Cuts at: 1342 1696 107
Size: 354 2619
Fragments arranged by size:
2619 354 317 241 156 136 129 118
114 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 609 1011 1128 1791 1924 2383 2592 609
Size: 402 117 663 133 459 209 2225
Fragments arranged by size:
2225 663 459 402 209 133 117
TatI w'GTAC_w
Cuts at: 3723 3723
Size: 4208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 870 902 904 936 990 1022 3725
Size: 32 492 32 2 32 54 32 2703
Cuts at: 3725 3757 346
Size: 32 797
Fragments arranged by size:
2703 797 492 54 32 32 32 32
32 2
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1086 1239 1086
Size: 153 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 1703 234
Size: 1469 2739
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4208
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BcgI BfrBI BpmI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI