Restriction map for
pF1KB7179
(Circular) (Six-base) MAPSORT of: pF1KB7179.seq Check: 3469 from: 1 to: 4550
pF1KB7179 4550 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 826 2375 2807 3554 3822 473
Size: 353 1549 432 747 268 1201
Fragments arranged by size:
1549 1201 747 432 353 268
AflIII A'CryG_T
Cuts at: 1139 1902 3006 3983 1139
Size: 763 1104 977 1706
Fragments arranged by size:
1706 1104 977 763
AgeI A'CCGG_T
Cuts at: 2938 2938
Size: 4550
AleI CACnn'nnGTG
Cuts at: 508 1082 508
Size: 574 3976
AlwNI CAG_nnn'CTG
Cuts at: 496 1855 3422 496
Size: 1359 1567 1624
Fragments arranged by size:
1624 1567 1359
ApaLI G'TGCA_C
Cuts at: 1361 3320 1361
Size: 1959 2591
ApoI r'AATT_y
Cuts at: 332 1554 3903 4159 332
Size: 1222 2349 256 723
Fragments arranged by size:
2349 1222 723 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 1570 1570
Size: 4550
BamHI G'GATC_C
Cuts at: 353 425 1575 353
Size: 72 1150 3328
Fragments arranged by size:
3328 1150 72
BanI G'GyrC_C
Cuts at: 1104 1566 1980 2228 2263 1104
Size: 462 414 248 35 3391
Fragments arranged by size:
3391 462 414 248 35
BanII G_rGCy'C
Cuts at: 1564 2594 1564
Size: 1030 3520
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbvCI CC'TCA_GC
Cuts at: 1183 1183
Size: 4550
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3209 4550 2448
Size: 761 1341 2448
Fragments arranged by size:
2448 1341 761
BclI T'GATC_A
Cuts at: 533 1034 1526 2070 533
Size: 501 492 544 3013
Fragments arranged by size:
3013 544 501 492
BglI GCCn_nnn'nGGC
Cuts at: 1027 1027
Size: 4550
BglII A'GATC_T
Cuts at: 684 2065 4102 684
Size: 1381 2037 1132
Fragments arranged by size:
2037 1381 1132
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4550
Bme1580I G_kGCm'C
Cuts at: 998 1365 2175 2268 3324 998
Size: 367 810 93 1056 2224
Fragments arranged by size:
2224 1056 810 367 93
BmrI ACTGGGnnnn_n'
Cuts at: 425 1122 1762 1843 2178 4322 425
Size: 697 640 81 335 2144 653
Fragments arranged by size:
2144 697 653 640 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
Bpu10I CC'TnA_GC
Cuts at: 1183 1183
Size: 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2605 3097 3395 1726
Size: 879 492 298 2881
Fragments arranged by size:
2881 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 918 4254 918
Size: 3336 1214
BsaAI yAC'GTr
Cuts at: 386 1903 386
Size: 1517 3033
BsaBI GATnn'nnATC
Cuts at: 6 377 1072 2064 4101 6
Size: 371 695 992 2037 455
Fragments arranged by size:
2037 992 695 455 371
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 901 1739 2260 2938 3212 3359 3770 901
Size: 838 521 678 274 147 411 1681
Fragments arranged by size:
1681 838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 896 926 896
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 825 829 863 825
Size: 4 34 4512
Fragments arranged by size:
4512 34 4
BseYI C'CCAG_C
Cuts at: 891 1975 2733 3310 3806 891
Size: 1084 758 577 496 1635
Fragments arranged by size:
1635 1084 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1102 2092 2138 3346 104
Size: 998 990 46 1208 1308
Fragments arranged by size:
1308 1208 998 990 46
BsiHKAI G_wGCw'C
Cuts at: 1365 1564 2342 2532 3324 1365
Size: 199 778 190 792 2591
Fragments arranged by size:
2591 792 778 199 190
BsmBI CGTCTCn'nnnn_
Cuts at: 172 172
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 998 1365 1564 2175 2268 2342 2532 2594 3324
Size: 367 199 611 93 74 190 62 730
Cuts at: 3324 998
Size: 2224
Fragments arranged by size:
2224 730 611 367 199 190 93 74
62
BspEI T'CCGG_A
Cuts at: 901 3770 901
Size: 2869 1681
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 1814 2116 2497 1600
Size: 214 302 381 3653
Fragments arranged by size:
3653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1206 1540 2842 2896 4380 4545 50
Size: 1156 334 1302 54 1484 165 55
Fragments arranged by size:
1484 1302 1156 334 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 2462
Size: 4550
BsrFI r'CCGG_y
Cuts at: 549 978 1101 2548 2729 2938 549
Size: 429 123 1447 181 209 2161
Fragments arranged by size:
2161 1447 429 209 181 123
BssSI C'ACGA_G
Cuts at: 439 2821 3179 439
Size: 2382 358 1810
Fragments arranged by size:
2382 1810 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 936 1084 936
Size: 148 4402
BstYI r'GATC_y
Cuts at: 1 143 353 425 684 1575 2042 2065 2400
Size: 142 210 72 259 891 467 23 335
Cuts at: 2400 2646 3658 3744 3756 4102 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 891 467 449 346 335 259 246
210 142 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 1340 1340
Size: 4550
BtgI C'CryG_G
Cuts at: 723 723
Size: 4550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1383 2500 2681 2844 1383
Size: 1117 181 163 3089
Fragments arranged by size:
3089 1117 181 163
BtsI GCAGTG_nn'
Cuts at: 616 1046 1793 616
Size: 430 747 3373
Fragments arranged by size:
3373 747 430
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3114 2256
Size: 858 3692
EaeI y'GGCC_r
Cuts at: 551 938 1099 2135 2309 2700 2727 4072 551
Size: 387 161 1036 174 391 27 1345 1029
Fragments arranged by size:
1345 1036 1029 391 387 174 161 27
EagI C'GGCC_G
Cuts at: 1099 2135 1099
Size: 1036 3514
EarI CTCTTCn'nnn_
Cuts at: 248 802 2573 2783 248
Size: 554 1771 210 2015
Fragments arranged by size:
2015 1771 554 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 346 2758 3068 3214 4360 346
Size: 2412 310 146 1146 536
Fragments arranged by size:
2412 1146 536 310 146
EcoICRI GAG'CTC
Cuts at: 1562 1562
Size: 4550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 826 2375 2807 3554 3822 473
Size: 353 1549 432 747 268 1201
Fragments arranged by size:
1549 1201 747 432 353 268
EcoO109I rG'GnC_Cy
Cuts at: 815 1689 815
Size: 874 3676
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 1984 2232 3254 4233 1984
Size: 248 1022 979 2301
Fragments arranged by size:
2301 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 870 896 902 914 928 946 2392
Size: 32 369 26 6 12 14 18 1446
Cuts at: 2392 2422 2424 2454 469
Size: 30 2 30 2565
Fragments arranged by size:
2565 1446 369 32 30 30 26 18
14 12 6 2
HincII GTy'rAC
Cuts at: 834 1055 1589 834
Size: 221 534 3795
Fragments arranged by size:
3795 534 221
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3221 3405 3221
Size: 184 4366
MscI TGG'CCA
Cuts at: 940 2311 940
Size: 1371 3179
MslI CAynn'nnrTG
Cuts at: 508 1063 1066 1082 1504 2539 2666 508
Size: 555 3 16 422 1035 127 2392
Fragments arranged by size:
2392 1035 555 422 127 16 3
MspA1I CmG'CkG
Cuts at: 680 736 1661 1975 2335 3348 3593 680
Size: 56 925 314 360 1013 245 1637
Fragments arranged by size:
1637 1013 925 360 314 245 56
NaeI GCC'GGC
Cuts at: 980 2731 980
Size: 1751 2799
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 723 723
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 978 2729 978
Size: 1751 2799
NspI r_CATG'y
Cuts at: 732 1143 1603 2103 3010 732
Size: 411 460 500 907 2272
Fragments arranged by size:
2272 907 500 460 411
PasI CC'CwG_GG
Cuts at: 918 918
Size: 4550
PciI A'CATG_T
Cuts at: 1139 3006 1139
Size: 1867 2683
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4550
PfoI T'CCnGG_A
Cuts at: 631 631
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PmlI CAC'GTG
Cuts at: 386 386
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PpuMI rG'GwC_Cy
Cuts at: 815 815
Size: 4550
PstI C_TGCA'G
Cuts at: 177 290 1597 2282 177
Size: 113 1307 685 2445
Fragments arranged by size:
2445 1307 685 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 1975 2335 1975
Size: 360 4190
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 1564 1564
Size: 4550
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 248 2573 2783 248
Size: 2325 210 2015
Fragments arranged by size:
2325 2015 210
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 173 286 1593 2278 3271 3462 33
Size: 140 113 1307 685 993 191 1121
Fragments arranged by size:
1307 1121 993 685 191 140 113
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 1705 2620 3112 3374 1705
Size: 915 492 262 2881
Fragments arranged by size:
2881 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 732 1603 2103 732
Size: 871 500 3179
Fragments arranged by size:
3179 871 500
SspI AAT'ATT
Cuts at: 1389 1389
Size: 4550
StyI C'CwwG_G
Cuts at: 671 723 1436 1684 2038 671
Size: 52 713 248 354 3183
Fragments arranged by size:
3183 713 354 248 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 1159 1490 2133 2266 2725 2934 407
Size: 752 331 643 133 459 209 2023
Fragments arranged by size:
2023 752 643 459 331 209 133
TatI w'GTAC_w
Cuts at: 1367 1394 4065 1367
Size: 27 2671 1852
Fragments arranged by size:
2671 1852 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 417 449 4067 4099 417
Size: 32 3618 32 868
Fragments arranged by size:
3618 868 32 32
Tth111I GACn'n_nGTC
Cuts at: 457 2347 457
Size: 1890 2660
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4550
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI