Restriction map for
pF1KB7186
(Circular) (Six-base) MAPSORT of: pF1KB7186.seq Check: 3730 from: 1 to: 4424
pF1KB7186 4424 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1277 1277
Size: 4424
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 1229 2249 2681 3428 3696 957
Size: 272 1020 432 747 268 1685
Fragments arranged by size:
1685 1020 747 432 272 268
AflIII A'CryG_T
Cuts at: 1022 1776 2880 3857 1022
Size: 754 1104 977 1589
Fragments arranged by size:
1589 1104 977 754
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AleI CACnn'nnGTG
Cuts at: 616 616
Size: 4424
AlwNI CAG_nnn'CTG
Cuts at: 534 753 852 980 1729 3296 534
Size: 219 99 128 749 1567 1662
Fragments arranged by size:
1662 1567 749 219 128 99
ApaI G_GGCC'C
Cuts at: 271 1415 271
Size: 1144 3280
ApaLI G'TGCA_C
Cuts at: 1403 3194 1403
Size: 1791 2633
ApoI r'AATT_y
Cuts at: 1225 1428 3777 4033 1225
Size: 203 2349 256 1616
Fragments arranged by size:
2349 1616 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 126 232 247 263 270 1444 126
Size: 106 15 16 7 1174 3106
Fragments arranged by size:
3106 1174 106 16 15 7
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 1440 1854 2102 2137 1440
Size: 414 248 35 3727
Fragments arranged by size:
3727 414 248 35
BanII G_rGCy'C
Cuts at: 133 218 233 248 264 271 287 895 1019
Size: 85 15 15 16 7 16 608 124
Cuts at: 1019 1415 1438 2468 133
Size: 396 23 1030 2089
Fragments arranged by size:
2089 1030 608 396 124 85 23 16
16 15 15 7
BbeI G_GCGC'C
Cuts at: 1858 2106 1858
Size: 248 4176
BbsI GAAGACnn'nnnn_
Cuts at: 928 928
Size: 4424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4390
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglII A'GATC_T
Cuts at: 1939 3976 1939
Size: 2037 2387
BlpI GC'TnA_GC
Cuts at: 535 628 1099 1536 535
Size: 93 471 437 3423
Fragments arranged by size:
3423 471 437 93
Bme1580I G_kGCm'C
Cuts at: 271 308 1368 1407 1415 2049 2142 3198 271
Size: 37 1060 39 8 634 93 1056 1497
Fragments arranged by size:
1497 1060 1056 634 93 39 37 8
BmgBI CAC'GTC
Cuts at: 564 564
Size: 4424
BmrI ACTGGGnnnn_n'
Cuts at: 428 1335 1636 1717 2052 4196 428
Size: 907 301 81 335 2144 656
Fragments arranged by size:
2144 907 656 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 2791 198
Size: 2593 1831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 748 1033 1174 410
Size: 338 285 141 3660
Fragments arranged by size:
3660 338 285 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 1600 2479 2971 3269 312
Size: 1288 879 492 298 1467
Fragments arranged by size:
1467 1288 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 428 678 4128 428
Size: 250 3450 724
Fragments arranged by size:
3450 724 250
BsaAI yAC'GTr
Cuts at: 1023 1346 1777 1023
Size: 323 431 3670
Fragments arranged by size:
3670 431 323
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 1855 2103 2805 4301 1855
Size: 248 702 1496 1978
Fragments arranged by size:
1978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 218 1144 1613 2134 2812 3086 3233 3644 218
Size: 926 469 521 678 274 147 411 998
Fragments arranged by size:
998 926 678 521 469 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 408 438 408
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 1163 454
Size: 709 3715
BseYI C'CCAG_C
Cuts at: 654 1040 1129 1284 1849 2607 3184 3680 654
Size: 386 89 155 565 758 577 496 1398
Fragments arranged by size:
1398 758 577 565 496 386 155 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4424
BsiEI CG_ry'CG
Cuts at: 104 997 1966 2012 3220 104
Size: 893 969 46 1208 1308
Fragments arranged by size:
1308 1208 969 893 46
BsiHKAI G_wGCw'C
Cuts at: 895 1142 1407 1438 2216 2406 3198 895
Size: 247 265 31 778 190 792 2121
Fragments arranged by size:
2121 792 778 265 247 190 31
BsmI GAATG_Cn'
Cuts at: 646 646
Size: 4424
BsmBI CGTCTCn'nnnn_
Cuts at: 554 554
Size: 4424
Bsp1286I G_dGCh'C
Cuts at: 133 218 233 248 264 271 287 308 895
Size: 85 15 15 16 7 16 21 587
Cuts at: 895 1019 1142 1368 1407 1415 1438 2049 2142
Size: 124 123 226 39 8 23 611 93
Cuts at: 2142 2216 2406 2468 3198 133
Size: 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 587 226 190 124 123
93 85 74 62 39 23 21 16
16 15 15 8 7
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 3600 3600
Size: 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 923 1277 1474 1688 1990 2371 923
Size: 354 197 214 302 381 2976
Fragments arranged by size:
2976 381 354 302 214 197
BsrBI CCG'CTC
Cuts at: 50 447 2716 2770 4254 4419 50
Size: 397 2269 54 1484 165 55
Fragments arranged by size:
2269 1484 397 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2336 2336
Size: 4424
BsrFI r'CCGG_y
Cuts at: 2422 2603 2812 2422
Size: 181 209 4034
Fragments arranged by size:
4034 209 181
BsrGI T'GTAC_A
Cuts at: 578 578
Size: 4424
BssSI C'ACGA_G
Cuts at: 724 838 2695 3053 724
Size: 114 1857 358 2095
Fragments arranged by size:
2095 1857 358 114
BstAPI GCAn_nnn'nTGC
Cuts at: 483 753 1801 483
Size: 270 1048 3106
Fragments arranged by size:
3106 1048 270
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstYI r'GATC_y
Cuts at: 1 468 1307 1449 1916 1939 2274 2520 3532
Size: 467 839 142 467 23 335 246 1012
Cuts at: 3532 3618 3630 3976 1
Size: 86 12 346 449
Fragments arranged by size:
1012 839 467 467 449 346 335 246
142 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 695 695
Size: 4424
BtgI C'CryG_G
Cuts at: 493 1001 1368 493
Size: 508 367 3549
Fragments arranged by size:
3549 508 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2374 2555 2718 2374
Size: 181 163 4080
Fragments arranged by size:
4080 181 163
BtsI GCAGTG_nn'
Cuts at: 950 1407 1667 950
Size: 457 260 3707
Fragments arranged by size:
3707 457 260
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DraIII CAC_nnn'GTG
Cuts at: 1374 1374
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 568 2130 2988 568
Size: 1562 858 2004
Fragments arranged by size:
2004 1562 858
EaeI y'GGCC_r
Cuts at: 830 994 2009 2183 2574 2601 3946 830
Size: 164 1015 174 391 27 1345 1308
Fragments arranged by size:
1345 1308 1015 391 174 164 27
EagI C'GGCC_G
Cuts at: 994 2009 994
Size: 1015 3409
EarI CTCTTCn'nnn_
Cuts at: 252 529 2447 2657 252
Size: 277 1918 210 2019
Fragments arranged by size:
2019 1918 277 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 2942 3088 4234 2632
Size: 310 146 1146 2822
Fragments arranged by size:
2822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 893 1436 893
Size: 543 3881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 748 957 1033 1174 1229 2249 2681 3428
Size: 338 209 76 141 55 1020 432 747
Cuts at: 3428 3696 410
Size: 268 1138
Fragments arranged by size:
1138 1020 747 432 338 268 209 141
76 55
EcoO109I rG'GnC_Cy
Cuts at: 267 404 1411 1412 1563 267
Size: 137 1007 1 151 3128
Fragments arranged by size:
3128 1007 151 137 1
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 352 1163 1858 2106 3128 4107 352
Size: 811 695 248 1022 979 669
Fragments arranged by size:
1022 979 811 695 669 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 440 453 485 693 725 893 925 2266
Size: 32 13 32 208 32 168 32 1341
Cuts at: 2266 2296 2298 2328 408
Size: 30 2 30 2504
Fragments arranged by size:
2504 1341 208 168 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 1463 1463
Size: 4424
KasI G'GCGC_C
Cuts at: 1854 2102 1854
Size: 248 4176
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1101 3095 3279 1101
Size: 1994 184 2246
Fragments arranged by size:
2246 1994 184
MscI TGG'CCA
Cuts at: 832 2185 832
Size: 1353 3071
MslI CAynn'nnrTG
Cuts at: 616 2413 2540 616
Size: 1797 127 2500
Fragments arranged by size:
2500 1797 127
MspA1I CmG'CkG
Cuts at: 226 495 753 1098 1288 1535 1849 2209 3222
Size: 269 258 345 190 247 314 360 1013
Cuts at: 3222 3467 226
Size: 245 1183
Fragments arranged by size:
1183 1013 360 345 314 269 258 247
245 190
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 4424
NarI GG'CG_CC
Cuts at: 1855 2103 1855
Size: 248 4176
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 4424
NspI r_CATG'y
Cuts at: 579 1477 1977 2884 579
Size: 898 500 907 2119
Fragments arranged by size:
2119 907 898 500
PasI CC'CwG_GG
Cuts at: 741 1030 741
Size: 289 4135
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PfoI T'CCnGG_A
Cuts at: 1010 1010
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PmlI CAC'GTG
Cuts at: 1023 1346 1023
Size: 323 4101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PspOMI G'GGCC_C
Cuts at: 267 1411 267
Size: 1144 3280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 940 972 940
Size: 32 4392
PstI C_TGCA'G
Cuts at: 758 920 1118 1274 1471 2156 758
Size: 162 198 156 197 685 3026
Fragments arranged by size:
3026 685 198 197 162 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 753 1098 1288 1849 2209 753
Size: 345 190 561 360 2968
Fragments arranged by size:
2968 561 360 345 190
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 895 1438 895
Size: 543 3881
SacII CC_GC'GG
Cuts at: 496 496
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 252 529 2447 2657 252
Size: 277 1918 210 2019
Fragments arranged by size:
2019 1918 277 210
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SexAI A'CCwGG_T
Cuts at: 668 668
Size: 4424
SfcI C'TryA_G
Cuts at: 33 754 916 1114 1270 1467 2152 3145 3336
Size: 721 162 198 156 197 685 993 191
Cuts at: 3336 33
Size: 1121
Fragments arranged by size:
1121 993 721 685 198 197 191 162
156
SfoI GGC'GCC
Cuts at: 1856 2104 1856
Size: 248 4176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 128 234 265 1446 128
Size: 106 31 1181 3106
Fragments arranged by size:
3106 1181 106 31
SmlI C'TyrA_G
Cuts at: 291 1579 2494 2986 3248 291
Size: 1288 915 492 262 1467
Fragments arranged by size:
1467 1288 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
SrfI GCCC'GGGC
Cuts at: 128 234 128
Size: 106 4318
StyI C'CwwG_G
Cuts at: 1059 1217 1298 1558 1912 1059
Size: 158 81 260 354 3571
Fragments arranged by size:
3571 354 260 158 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2007 2140 2599 2808 2007
Size: 133 459 209 3623
Fragments arranged by size:
3623 459 209 133
TatI w'GTAC_w
Cuts at: 578 3939 578
Size: 3361 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4392
Tth111I GACn'n_nGTC
Cuts at: 420 2221 420
Size: 1801 2623
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 1027 1919 1008
Size: 19 892 3513
Fragments arranged by size:
3513 892 19
XmaI C'CCGG_G
Cuts at: 126 232 263 1444 126
Size: 106 31 1181 3106
Fragments arranged by size:
3106 1181 106 31
XmnI GAAnn'nnTTC
Cuts at: 970 970
Size: 4424
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI Bpu10I BsiWI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PpuMI PshAI PsiI PspXI
SanDI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
ZraI