Restriction map for
pF1KB7187
(Circular) (Six-base) MAPSORT of: pF1KB7187.seq Check: 4602 from: 1 to: 4730
pF1KB7187 4730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1289 1289
Size: 4730
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2555 2987 3734 4002 1693
Size: 862 432 747 268 2421
Fragments arranged by size:
2421 862 747 432 268
AflIII A'CryG_T
Cuts at: 2082 3186 4163 2082
Size: 1104 977 2649
Fragments arranged by size:
2649 1104 977
AgeI A'CCGG_T
Cuts at: 3118 3118
Size: 4730
AlwNI CAG_nnn'CTG
Cuts at: 310 601 2035 3602 310
Size: 291 1434 1567 1438
Fragments arranged by size:
1567 1438 1434 291
ApaI G_GGCC'C
Cuts at: 133 1040 133
Size: 907 3823
ApaLI G'TGCA_C
Cuts at: 3500 3500
Size: 4730
ApoI r'AATT_y
Cuts at: 1492 1734 4083 4339 1492
Size: 242 2349 256 1883
Fragments arranged by size:
2349 1883 256 242
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 132 508 1039 1750 132
Size: 376 531 711 3112
Fragments arranged by size:
3112 711 531 376
BamHI G'GATC_C
Cuts at: 1755 1755
Size: 4730
BanI G'GyrC_C
Cuts at: 336 397 697 1746 2160 2408 2443 336
Size: 61 300 1049 414 248 35 2623
Fragments arranged by size:
2623 1049 414 300 248 61 35
BanII G_rGCy'C
Cuts at: 133 595 622 710 1040 1390 1514 1555 1744
Size: 462 27 88 330 350 124 41 189
Cuts at: 1744 2774 133
Size: 1030 2089
Fragments arranged by size:
2089 1030 462 350 330 189 124 88
41 27
BbeI G_GCGC'C
Cuts at: 340 2164 2412 340
Size: 1824 248 2658
Fragments arranged by size:
2658 1824 248
BbsI GAAGACnn'nnnn_
Cuts at: 1699 1699
Size: 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1098 1132 1098
Size: 34 4696
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3389 4730 2628
Size: 761 1341 2628
Fragments arranged by size:
2628 1341 761
BclI T'GATC_A
Cuts at: 2250 2250
Size: 4730
BglI GCCn_nnn'nGGC
Cuts at: 193 202 1190 193
Size: 9 988 3733
Fragments arranged by size:
3733 988 9
BglII A'GATC_T
Cuts at: 777 2245 4282 777
Size: 1468 2037 1225
Fragments arranged by size:
2037 1468 1225
BlpI GC'TnA_GC
Cuts at: 589 1842 589
Size: 1253 3477
Bme1580I G_kGCm'C
Cuts at: 133 700 1016 1040 2355 2448 3504 133
Size: 567 316 24 1315 93 1056 1359
Fragments arranged by size:
1359 1315 1056 567 316 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1028 1942 2023 2358 4502 1028
Size: 914 81 335 2144 1256
Fragments arranged by size:
2144 1256 914 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 4730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 724 870 1146 472
Size: 252 146 276 4056
Fragments arranged by size:
4056 276 252 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1441 1588 1684 1906 2785 3277 3575 650
Size: 791 147 96 222 879 492 298 1805
Fragments arranged by size:
1805 879 791 492 298 222 147 96
BsaI GGTCTCn'nnnn_
Cuts at: 832 977 4434 832
Size: 145 3457 1128
Fragments arranged by size:
3457 1128 145
BsaAI yAC'GTr
Cuts at: 2083 2083
Size: 4730
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 325 337 2161 2409 3111 4607 325
Size: 12 1824 248 702 1496 448
Fragments arranged by size:
1824 1496 702 448 248 12
BsaWI w'CCGG_w
Cuts at: 1496 1919 2440 3118 3392 3539 3950 1496
Size: 423 521 678 274 147 411 2276
Fragments arranged by size:
2276 678 521 423 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1144 1174 1144
Size: 30 4700
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 955 958 967 1636 1639 449
Size: 506 3 9 669 3 3540
Fragments arranged by size:
3540 669 506 9 3 3
BseYI C'CCAG_C
Cuts at: 310 594 2155 2913 3490 3986 310
Size: 284 1561 758 577 496 1054
Fragments arranged by size:
1561 1054 758 577 496 284
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3526 104
Size: 2168 46 1208 1308
Fragments arranged by size:
2168 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1514 1581 1744 2522 2712 3504 1514
Size: 67 163 778 190 792 2740
Fragments arranged by size:
2740 792 778 190 163 67
Bsp1286I G_dGCh'C
Cuts at: 133 595 622 700 710 1016 1040 1390 1514
Size: 462 27 78 10 306 24 350 124
Cuts at: 1514 1555 1581 1744 2355 2448 2522 2712 2774
Size: 41 26 163 611 93 74 190 62
Cuts at: 2774 3504 133
Size: 730 1359
Fragments arranged by size:
1359 730 611 462 350 306 190 163
124 93 78 74 62 41 27 26
24 10
BspEI T'CCGG_A
Cuts at: 1496 3950 1496
Size: 2454 2276
BspHI T'CATG_A
Cuts at: 1418 3906 1418
Size: 2488 2242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1289 1460 1780 1994 2296 2677 1289
Size: 171 320 214 302 381 3342
Fragments arranged by size:
3342 381 320 302 214 171
BsrBI CCG'CTC
Cuts at: 50 3022 3076 4560 4725 50
Size: 2972 54 1484 165 55
Fragments arranged by size:
2972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 2642 1541
Size: 1101 3629
BsrFI r'CCGG_y
Cuts at: 201 295 394 714 1217 2728 2909 3118 201
Size: 94 99 320 503 1511 181 209 1813
Fragments arranged by size:
1813 1511 503 320 209 181 99 94
BssSI C'ACGA_G
Cuts at: 499 3001 3359 499
Size: 2502 358 1870
Fragments arranged by size:
2502 1870 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1181 2107 1181
Size: 926 3804
BstBI TT'CG_AA
Cuts at: 746 3091 746
Size: 2345 2385
BstEII G'GTnAC_C
Cuts at: 390 797 1678 390
Size: 407 881 3442
Fragments arranged by size:
3442 881 407
BstYI r'GATC_y
Cuts at: 1 777 1755 2222 2245 2580 2826 3838 3924
Size: 776 978 467 23 335 246 1012 86
Cuts at: 3924 3936 4282 1
Size: 12 346 449
Fragments arranged by size:
1012 978 776 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 539 1123 539
Size: 584 4146
BtgI C'CryG_G
Cuts at: 107 1382 107
Size: 1275 3455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 476 1101 2680 2861 3024 476
Size: 625 1579 181 163 2182
Fragments arranged by size:
2182 1579 625 181 163
BtsI GCAGTG_nn'
Cuts at: 151 1194 1973 151
Size: 1043 779 2908
Fragments arranged by size:
2908 1043 779
DraI TTT'AAA
Cuts at: 1729 1729
Size: 4730
DraIII CAC_nnn'GTG
Cuts at: 1181 1181
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3294 2436
Size: 858 3872
EaeI y'GGCC_r
Cuts at: 353 1260 1379 2315 2489 2880 2907 4252 353
Size: 907 119 936 174 391 27 1345 831
Fragments arranged by size:
1345 936 907 831 391 174 119 27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 230 359 932 944 1240 1420 2753 2963 230
Size: 129 573 12 296 180 1333 210 1997
Fragments arranged by size:
1997 1333 573 296 210 180 129 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2938 3248 3394 4540 2938
Size: 310 146 1146 3128
Fragments arranged by size:
3128 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1512 1742 1512
Size: 230 4500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 724 870 1146 1693 2555 2987 3734 4002
Size: 252 146 276 547 862 432 747 268
Cuts at: 4002 472
Size: 1200
Fragments arranged by size:
1200 862 747 547 432 276 268 252
146
EcoNI CCTnn'n_nnAGG
Cuts at: 1296 1618 1296
Size: 322 4408
EcoO109I rG'GnC_Cy
Cuts at: 129 471 504 1036 1127 1289 1869 129
Size: 342 33 532 91 162 580 2990
Fragments arranged by size:
2990 580 532 342 162 91 33
EcoRI G'AATT_C
Cuts at: 1734 1734
Size: 4730
EcoRV GAT'ATC
Cuts at: 726 726
Size: 4730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4730
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 340 2164 2412 3434 4413 340
Size: 1824 248 1022 979 657
Fragments arranged by size:
1824 1022 979 657 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 1144 1176 1513 1545 1612 1644 2572
Size: 32 802 32 337 32 67 32 928
Cuts at: 2572 2602 2604 2634 310
Size: 30 2 30 2406
Fragments arranged by size:
2406 928 802 337 67 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
KasI G'GCGC_C
Cuts at: 336 2160 2408 336
Size: 1824 248 2658
Fragments arranged by size:
2658 1824 248
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3401 3585 3401
Size: 184 4546
MscI TGG'CCA
Cuts at: 2491 2491
Size: 4730
MslI CAynn'nnrTG
Cuts at: 1562 2719 2846 1562
Size: 1157 127 3446
Fragments arranged by size:
3446 1157 127
MspA1I CmG'CkG
Cuts at: 307 458 557 1841 2155 2515 3528 3773 307
Size: 151 99 1284 314 360 1013 245 1264
Fragments arranged by size:
1284 1264 1013 360 314 245 151 99
NaeI GCC'GGC
Cuts at: 203 297 2911 203
Size: 94 2614 2022
Fragments arranged by size:
2614 2022 94
NarI GG'CG_CC
Cuts at: 337 2161 2409 337
Size: 1824 248 2658
Fragments arranged by size:
2658 1824 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4730
NgoMIV G'CCGG_C
Cuts at: 201 295 2909 201
Size: 94 2614 2022
Fragments arranged by size:
2614 2022 94
NspI r_CATG'y
Cuts at: 1072 1783 2283 3190 1072
Size: 711 500 907 2612
Fragments arranged by size:
2612 907 711 500
PasI CC'CwG_GG
Cuts at: 793 793
Size: 4730
PciI A'CATG_T
Cuts at: 3186 3186
Size: 4730
PflMI CCAn_nnn'nTGG
Cuts at: 310 1064 310
Size: 754 3976
PfoI T'CCnGG_A
Cuts at: 847 1592 847
Size: 745 3985
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4698
PpuMI rG'GwC_Cy
Cuts at: 504 504
Size: 4730
PspOMI G'GGCC_C
Cuts at: 129 1036 129
Size: 907 3823
PstI C_TGCA'G
Cuts at: 377 1300 1777 2462 377
Size: 923 477 685 2645
Fragments arranged by size:
2645 923 685 477
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 2155 2515 2155
Size: 360 4370
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1514 1744 1514
Size: 230 4500
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 230 1420 2753 2963 230
Size: 1190 1333 210 1997
Fragments arranged by size:
1997 1333 1190 210
SbfI CC_TGCA'GG
Cuts at: 1777 1777
Size: 4730
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SexAI A'CCwGG_T
Cuts at: 1271 1271
Size: 4730
SfcI C'TryA_G
Cuts at: 33 373 545 1296 1773 2458 3451 3642 33
Size: 340 172 751 477 685 993 191 1121
Fragments arranged by size:
1121 993 751 685 477 340 191 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 193 193
Size: 4730
SfoI GGC'GCC
Cuts at: 338 2162 2410 338
Size: 1824 248 2658
Fragments arranged by size:
2658 1824 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 134 1041 1752 134
Size: 907 711 3112
Fragments arranged by size:
3112 907 711
SmlI C'TyrA_G
Cuts at: 665 1456 1603 1663 1885 2800 3292 3554 665
Size: 791 147 60 222 915 492 262 1841
Fragments arranged by size:
1841 915 791 492 262 222 147 60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 1072 1783 2283 1072
Size: 711 500 3519
Fragments arranged by size:
3519 711 500
SrfI GCCC'GGGC
Cuts at: 134 134
Size: 4730
StuI AGG'CCT
Cuts at: 428 428
Size: 4730
StyI C'CwwG_G
Cuts at: 107 611 623 1864 2218 107
Size: 504 12 1241 354 2619
Fragments arranged by size:
2619 1241 504 354 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1089 1574 2313 2446 2905 3114 1089
Size: 485 739 133 459 209 2705
Fragments arranged by size:
2705 739 485 459 209 133
TatI w'GTAC_w
Cuts at: 4245 4245
Size: 4730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 4698
Tth111I GACn'n_nGTC
Cuts at: 2527 2527
Size: 4730
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2225 2225
Size: 4730
XmaI C'CCGG_G
Cuts at: 132 1039 1750 132
Size: 907 711 3112
Fragments arranged by size:
3112 907 711
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI