Restriction map for
pF1KB7197
(Circular) (Six-base) MAPSORT of: pF1KB7197.seq Check: 3685 from: 1 to: 4229
pF1KB7197 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1125 2054 2486 3233 3501 1125
Size: 929 432 747 268 1853
Fragments arranged by size:
1853 929 747 432 268
AfeI AGC'GCT
Cuts at: 195 1087 195
Size: 892 3337
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 714 493
Size: 221 4008
AlwNI CAG_nnn'CTG
Cuts at: 1534 3101 1534
Size: 1567 2662
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 680 1249 680
Size: 569 3660
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 603 636 603
Size: 33 4196
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 130 199 777 910 930 1245 1659 1907 1942
Size: 69 578 133 20 315 414 248 35
Cuts at: 1942 130
Size: 2417
Fragments arranged by size:
2417 578 414 315 248 133 69 35
20
BanII G_rGCy'C
Cuts at: 853 1243 2273 853
Size: 390 1030 2809
Fragments arranged by size:
2809 1030 390
BbeI G_GCGC'C
Cuts at: 134 203 914 934 1663 1911 134
Size: 69 711 20 729 248 2452
Fragments arranged by size:
2452 729 711 248 69 20
BbsI GAAGACnn'nnnn_
Cuts at: 355 355
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 608 1749 608
Size: 1141 3088
BglII A'GATC_T
Cuts at: 749 1744 3781 749
Size: 995 2037 1197
Fragments arranged by size:
2037 1197 995
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 653 782 1854 1947 3003 653
Size: 129 1072 93 1056 1879
Fragments arranged by size:
1879 1072 1056 129 93
BmgBI CAC'GTC
Cuts at: 446 446
Size: 4229
BmrI ACTGGGnnnn_n'
Cuts at: 741 1441 1522 1857 4001 741
Size: 700 81 335 2144 969
Fragments arranged by size:
2144 969 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 691
Size: 4229
Bpu10I CC'TnA_GC
Cuts at: 384 384
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 1582 1582
Size: 4229
BsaBI GATnn'nnATC
Cuts at: 6 613 1743 3780 6
Size: 607 1130 2037 455
Fragments arranged by size:
2037 1130 607 455
BsaHI Gr'CG_yC
Cuts at: 131 170 200 911 931 1660 1908 2610 4106
Size: 39 30 711 20 729 248 702 1496
Cuts at: 4106 131
Size: 254
Fragments arranged by size:
1496 729 711 702 254 248 39 30
20
BsaWI w'CCGG_w
Cuts at: 332 1418 1939 2617 2891 3038 3449 332
Size: 1086 521 678 274 147 411 1112
Fragments arranged by size:
1112 1086 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 190 300 715 721 1023 1132 1654 2412 2989
Size: 110 415 6 302 109 522 758 577
Cuts at: 2989 3485 190
Size: 496 934
Fragments arranged by size:
934 758 577 522 496 415 302 110
109 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1170 991
Size: 179 4050
BsiEI CG_ry'CG
Cuts at: 104 281 978 1122 1771 1817 3025 104
Size: 177 697 144 649 46 1208 1308
Fragments arranged by size:
1308 1208 697 649 177 144 46
BsiHKAI G_wGCw'C
Cuts at: 482 1058 1243 2021 2211 3003 482
Size: 576 185 778 190 792 1708
Fragments arranged by size:
1708 792 778 576 190 185
BsmBI CGTCTCn'nnnn_
Cuts at: 436 436
Size: 4229
Bsp1286I G_dGCh'C
Cuts at: 482 653 782 853 1058 1243 1854 1947 2021
Size: 171 129 71 205 185 611 93 74
Cuts at: 2021 2211 2273 3003 482
Size: 190 62 730 1708
Fragments arranged by size:
1708 730 611 205 190 185 171 129
93 74 71 62
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1279 1493 1795 2176 1279
Size: 214 302 381 3332
Fragments arranged by size:
3332 381 302 214
BsrBI CCG'CTC
Cuts at: 50 150 2521 2575 4059 4224 50
Size: 100 2371 54 1484 165 55
Fragments arranged by size:
2371 1484 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 114 127 977 2227 2408 2617 114
Size: 13 850 1250 181 209 1726
Fragments arranged by size:
1726 1250 850 209 181 13
BssHII G'CGCG_C
Cuts at: 224 224
Size: 4229
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 1115 1606 1115
Size: 491 3738
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstEII G'GTnAC_C
Cuts at: 741 741
Size: 4229
BstYI r'GATC_y
Cuts at: 1 749 1254 1721 1744 2079 2325 3337 3423
Size: 748 505 467 23 335 246 1012 86
Cuts at: 3423 3435 3781 1
Size: 12 346 449
Fragments arranged by size:
1012 748 505 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 636 636
Size: 4229
BtgI C'CryG_G
Cuts at: 107 290 1160 107
Size: 183 870 3176
Fragments arranged by size:
3176 870 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 534 1002 2179 2360 2523 534
Size: 468 1177 181 163 2240
Fragments arranged by size:
2240 1177 468 181 163
BtsI GCAGTG_nn'
Cuts at: 698 837 1472 698
Size: 139 635 3455
Fragments arranged by size:
3455 635 139
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DraIII CAC_nnn'GTG
Cuts at: 800 800
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 278 353 398 1119 1814 1988 2379 2406 3751
Size: 75 45 721 695 174 391 27 1345
Cuts at: 3751 278
Size: 756
Fragments arranged by size:
1345 756 721 695 391 174 75 45
27
EagI C'GGCC_G
Cuts at: 278 1119 1814 278
Size: 841 695 2693
Fragments arranged by size:
2693 841 695
EarI CTCTTCn'nnn_
Cuts at: 157 769 2252 2462 157
Size: 612 1483 210 1924
Fragments arranged by size:
1924 1483 612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 1084 2437 2747 2893 4039 114
Size: 970 1353 310 146 1146 304
Fragments arranged by size:
1353 1146 970 310 304 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1125 2054 2486 3233 3501 691
Size: 434 929 432 747 268 1419
Fragments arranged by size:
1419 929 747 434 432 268
EcoO109I rG'GnC_Cy
Cuts at: 573 627 1368 573
Size: 54 741 3434
Fragments arranged by size:
3434 741 54
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1107 2248 1107
Size: 1141 3088
FspI TGC'GCA
Cuts at: 829 2010 829
Size: 1181 3048
HaeII r_GCGC'y
Cuts at: 134 185 197 203 236 914 934 1089 1217
Size: 51 12 6 33 678 20 155 128
Cuts at: 1217 1663 1911 2933 3912 134
Size: 446 248 1022 979 451
Fragments arranged by size:
1022 979 678 451 446 248 155 128
51 33 20 12 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2071 2101 2103 2133 2071
Size: 30 2 30 4167
Fragments arranged by size:
4167 30 30 2
HincII GTy'rAC
Cuts at: 540 1268 540
Size: 728 3501
KasI G'GCGC_C
Cuts at: 130 199 910 930 1659 1907 130
Size: 69 711 20 729 248 2452
Fragments arranged by size:
2452 729 711 248 69 20
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2900 3084 2900
Size: 184 4045
MscI TGG'CCA
Cuts at: 400 1990 400
Size: 1590 2639
MslI CAynn'nnrTG
Cuts at: 343 529 661 667 796 889 2218 2345 343
Size: 186 132 6 129 93 1329 127 2227
Fragments arranged by size:
2227 1329 186 132 129 127 93 6
MspA1I CmG'CkG
Cuts at: 725 990 1340 1654 2014 3027 3272 725
Size: 265 350 314 360 1013 245 1682
Fragments arranged by size:
1682 1013 360 350 314 265 245
NaeI GCC'GGC
Cuts at: 129 2410 129
Size: 2281 1948
NarI GG'CG_CC
Cuts at: 131 200 911 931 1660 1908 131
Size: 69 711 20 729 248 2452
Fragments arranged by size:
2452 729 711 248 69 20
NcoI C'CATG_G
Cuts at: 107 107
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 127 2408 127
Size: 2281 1948
NotI GC'GGCC_GC
Cuts at: 1119 1119
Size: 4229
NspI r_CATG'y
Cuts at: 705 1282 1782 2689 705
Size: 577 500 907 2245
Fragments arranged by size:
2245 907 577 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 627 627
Size: 4229
PstI C_TGCA'G
Cuts at: 143 1276 1961 143
Size: 1133 685 2411
Fragments arranged by size:
2411 1133 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 725 1654 2014 725
Size: 929 360 2940
Fragments arranged by size:
2940 929 360
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 157 2252 2462 157
Size: 2095 210 1924
Fragments arranged by size:
2095 1924 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 139 1272 1957 2950 3141 33
Size: 106 1133 685 993 191 1121
Fragments arranged by size:
1133 1121 993 685 191 106
SfoI GGC'GCC
Cuts at: 132 201 912 932 1661 1909 132
Size: 69 711 20 729 248 2452
Fragments arranged by size:
2452 729 711 248 69 20
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SgrAI Cr'CCGG_yG
Cuts at: 127 127
Size: 4229
SmaI CCC'GGG
Cuts at: 682 1251 682
Size: 569 3660
SmlI C'TyrA_G
Cuts at: 1384 2299 2791 3053 1384
Size: 915 492 262 2560
Fragments arranged by size:
2560 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 705 1282 1782 705
Size: 577 500 3152
Fragments arranged by size:
3152 577 500
StyI C'CwwG_G
Cuts at: 107 596 854 1363 1717 107
Size: 489 258 509 354 2619
Fragments arranged by size:
2619 509 489 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2613 1812
Size: 133 459 209 3428
Fragments arranged by size:
3428 459 209 133
TatI w'GTAC_w
Cuts at: 3744 3744
Size: 4229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 435 660 1724 435
Size: 225 1064 2940
Fragments arranged by size:
2940 1064 225
XmaI C'CCGG_G
Cuts at: 680 1249 680
Size: 569 3660
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI BsaXI BseRI
BsiWI BsmI BsrGI BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI