Restriction map for
pF1KB7198
(Circular) (Six-base) MAPSORT of: pF1KB7198.seq Check: 8743 from: 1 to: 4502
pF1KB7198 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 629 629
Size: 4502
AccI GT'mk_AC
Cuts at: 1540 1540
Size: 4502
Acc65I G'GTAC_C
Cuts at: 368 1518 368
Size: 1150 3352
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 597 730 888 1132 2327 2759 3506 3774
Size: 151 133 158 244 1195 432 747 268
Cuts at: 3774 446
Size: 1174
Fragments arranged by size:
1195 1174 747 432 268 244 158 151
133
AfeI AGC'GCT
Cuts at: 250 1363 250
Size: 1113 3389
AflIII A'CryG_T
Cuts at: 404 1854 2958 3935 404
Size: 1450 1104 977 971
Fragments arranged by size:
1450 1104 977 971
AgeI A'CCGG_T
Cuts at: 410 2890 410
Size: 2480 2022
AlwNI CAG_nnn'CTG
Cuts at: 531 1293 1807 3374 531
Size: 762 514 1567 1659
Fragments arranged by size:
1659 1567 762 514
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 1342 1506 3855 4111 1342
Size: 164 2349 256 1733
Fragments arranged by size:
2349 1733 256 164
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 151 1232 1522 151
Size: 1081 290 3131
Fragments arranged by size:
3131 1081 290
BamHI G'GATC_C
Cuts at: 264 1527 264
Size: 1263 3239
BanI G'GyrC_C
Cuts at: 115 187 368 1518 1932 2180 2215 115
Size: 72 181 1150 414 248 35 2402
Fragments arranged by size:
2402 1150 414 248 181 72 35
BanII G_rGCy'C
Cuts at: 758 1271 1516 2546 758
Size: 513 245 1030 2714
Fragments arranged by size:
2714 1030 513 245
BbeI G_GCGC'C
Cuts at: 119 1936 2184 119
Size: 1817 248 2437
Fragments arranged by size:
2437 1817 248
BbsI GAAGACnn'nnnn_
Cuts at: 681 681
Size: 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 224 258 224
Size: 34 4468
BciVI GTATCCnnnnn_n'
Cuts at: 1113 2400 3161 4502 1113
Size: 1287 761 1341 1113
Fragments arranged by size:
1341 1287 1113 761
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 2127 2220 3276 2127
Size: 93 1056 3353
Fragments arranged by size:
3353 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1714 1795 2130 4274 1714
Size: 81 335 2144 1942
Fragments arranged by size:
2144 1942 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 357 2869 357
Size: 2512 1990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 486 438
Size: 48 4454
Bpu10I CC'TnA_GC
Cuts at: 1435 1435
Size: 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 1399 1678 2557 3049 3347 1393
Size: 6 279 879 492 298 2548
Fragments arranged by size:
2548 879 492 298 279 6
BsaI GGTCTCn'nnnn_
Cuts at: 4206 4206
Size: 4502
BsaAI yAC'GTr
Cuts at: 405 915 1855 405
Size: 510 940 3052
Fragments arranged by size:
3052 940 510
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 116 237 1933 2181 2883 4379 116
Size: 121 1696 248 702 1496 239
Fragments arranged by size:
1696 1496 702 248 239 121
BsaWI w'CCGG_w
Cuts at: 296 410 1691 2212 2890 3164 3311 3722 296
Size: 114 1281 521 678 274 147 411 1076
Fragments arranged by size:
1281 1076 678 521 411 274 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 838 868 553
Size: 30 255 30 4187
Fragments arranged by size:
4187 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 535 595 748 502
Size: 33 60 153 4256
Fragments arranged by size:
4256 153 60 33
BseYI C'CCAG_C
Cuts at: 734 926 1185 1355 1927 2685 3262 3758 734
Size: 192 259 170 572 758 577 496 1478
Fragments arranged by size:
1478 758 577 572 496 259 192 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 402 607 612 235
Size: 167 205 5 4125
Fragments arranged by size:
4125 205 167 5
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3298 104
Size: 1940 46 1208 1308
Fragments arranged by size:
1940 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1229 1271 1379 1516 2294 2484 3276 1229
Size: 42 108 137 778 190 792 2455
Fragments arranged by size:
2455 792 778 190 137 108 42
BsmBI CGTCTCn'nnnn_
Cuts at: 202 202
Size: 4502
Bsp1286I G_dGCh'C
Cuts at: 758 1229 1271 1379 1516 2127 2220 2294 2484
Size: 471 42 108 137 611 93 74 190
Cuts at: 2484 2546 3276 758
Size: 62 730 1984
Fragments arranged by size:
1984 730 611 471 190 137 108 93
74 62 42
BspEI T'CCGG_A
Cuts at: 3722 3722
Size: 4502
BspHI T'CATG_A
Cuts at: 1194 3678 1194
Size: 2484 2018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 629 1552 1766 2068 2449 380
Size: 249 923 214 302 381 2433
Fragments arranged by size:
2433 923 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 2794 2848 4332 4497 50
Size: 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 901 2414 901
Size: 1513 2989
BsrFI r'CCGG_y
Cuts at: 112 169 410 2500 2681 2890 112
Size: 57 241 2090 181 209 1724
Fragments arranged by size:
2090 1724 241 209 181 57
BsrGI T'GTAC_A
Cuts at: 860 1466 860
Size: 606 3896
BssSI C'ACGA_G
Cuts at: 2773 3131 2773
Size: 358 4144
BstAPI GCAn_nnn'nTGC
Cuts at: 1879 1879
Size: 4502
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 751 751
Size: 4502
BstYI r'GATC_y
Cuts at: 1 264 899 1527 1994 2017 2352 2598 3610
Size: 263 635 628 467 23 335 246 1012
Cuts at: 3610 3696 3708 4054 1
Size: 86 12 346 449
Fragments arranged by size:
1012 635 628 467 449 346 335 263
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1448 107
Size: 1341 3161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2452 2633 2796 2452
Size: 181 163 4158
Fragments arranged by size:
4158 181 163
BtsI GCAGTG_nn'
Cuts at: 393 624 865 1446 1745 393
Size: 231 241 581 299 3150
Fragments arranged by size:
3150 581 299 241 231
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 110 322 821 1301 2087 2261 2652 2679 4024
Size: 212 499 480 786 174 391 27 1345
Cuts at: 4024 110
Size: 588
Fragments arranged by size:
1345 786 588 499 480 391 212 174
27
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 1345 2525 2735 1345
Size: 1180 210 3112
Fragments arranged by size:
3112 1180 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2710 3020 3166 4312 2710
Size: 310 146 1146 2900
Fragments arranged by size:
2900 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1269 1514 1269
Size: 245 4257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 446 486 597 730 888 1132 2327 2759
Size: 8 40 111 133 158 244 1195 432
Cuts at: 2759 3506 3774 438
Size: 747 268 1166
Fragments arranged by size:
1195 1166 747 432 268 244 158 133
111 40 8
EcoNI CCTnn'n_nnAGG
Cuts at: 259 979 259
Size: 720 3782
EcoO109I rG'GnC_Cy
Cuts at: 1238 1641 1238
Size: 403 4099
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4502
FseI GG_CCGG'CC
Cuts at: 173 173
Size: 4502
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 119 252 1365 1936 2184 3206 4185 119
Size: 133 1113 571 248 1022 979 436
Fragments arranged by size:
1113 1022 979 571 436 248 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 2344 2374 2376 2406 249
Size: 32 2063 30 2 30 2345
Fragments arranged by size:
2345 2063 32 30 30 2
HincII GTy'rAC
Cuts at: 1323 1541 1323
Size: 218 4284
KasI G'GCGC_C
Cuts at: 115 1932 2180 115
Size: 1817 248 2437
Fragments arranged by size:
2437 1817 248
KpnI G_GTAC'C
Cuts at: 372 1522 372
Size: 1150 3352
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 1034 3173 3357 458
Size: 576 2139 184 1603
Fragments arranged by size:
2139 1603 576 184
MscI TGG'CCA
Cuts at: 324 1303 2263 324
Size: 979 960 2563
Fragments arranged by size:
2563 979 960
MslI CAynn'nnrTG
Cuts at: 1060 2491 2618 1060
Size: 1431 127 2944
Fragments arranged by size:
2944 1431 127
MspA1I CmG'CkG
Cuts at: 129 359 424 1338 1613 1927 2287 3300 3545
Size: 230 65 914 275 314 360 1013 245
Cuts at: 3545 129
Size: 1086
Fragments arranged by size:
1086 1013 914 360 314 275 245 230
65
NaeI GCC'GGC
Cuts at: 114 171 2683 114
Size: 57 2512 1933
Fragments arranged by size:
2512 1933 57
NarI GG'CG_CC
Cuts at: 116 1933 2181 116
Size: 1817 248 2437
Fragments arranged by size:
2437 1817 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 112 169 2681 112
Size: 57 2512 1933
Fragments arranged by size:
2512 1933 57
NspI r_CATG'y
Cuts at: 1467 1555 2055 2962 1467
Size: 88 500 907 3007
Fragments arranged by size:
3007 907 500 88
PasI CC'CwG_GG
Cuts at: 183 377 956 183
Size: 194 579 3729
Fragments arranged by size:
3729 579 194
PciI A'CATG_T
Cuts at: 2958 2958
Size: 4502
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PmlI CAC'GTG
Cuts at: 405 405
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PpuMI rG'GwC_Cy
Cuts at: 1238 1238
Size: 4502
PstI C_TGCA'G
Cuts at: 443 1495 1549 2234 443
Size: 1052 54 685 2711
Fragments arranged by size:
2711 1052 685 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 359 424 1338 1927 2287 359
Size: 65 914 589 360 2574
Fragments arranged by size:
2574 914 589 360 65
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 1271 1516 1271
Size: 245 4257
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SanDI GG'GwC_CC
Cuts at: 1238 1238
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 1345 2525 2735 1345
Size: 1180 210 3112
Fragments arranged by size:
3112 1180 210
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 811 4019 811
Size: 3208 1294
SfcI C'TryA_G
Cuts at: 33 439 1491 1545 2230 3223 3414 33
Size: 406 1052 54 685 993 191 1121
Fragments arranged by size:
1121 1052 993 685 406 191 54
SfoI GGC'GCC
Cuts at: 117 1934 2182 117
Size: 1817 248 2437
Fragments arranged by size:
2437 1817 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 1234 1524 1234
Size: 290 4212
SmlI C'TyrA_G
Cuts at: 1372 1414 1657 2572 3064 3326 1372
Size: 42 243 915 492 262 2548
Fragments arranged by size:
2548 915 492 262 243 42
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 431 1486 431
Size: 1055 3447
StyI C'CwwG_G
Cuts at: 107 1636 1990 107
Size: 1529 354 2619
Fragments arranged by size:
2619 1529 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 870 2085 2218 2677 2886 870
Size: 1215 133 459 209 2486
Fragments arranged by size:
2486 1215 459 209 133
TatI w'GTAC_w
Cuts at: 809 860 1466 4017 809
Size: 51 606 2551 1294
Fragments arranged by size:
2551 1294 606 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4470
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1997 1997
Size: 4502
XmaI C'CCGG_G
Cuts at: 1232 1522 1232
Size: 290 4212
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI