Restriction map for
pF1KB7201
(Circular) (Six-base) MAPSORT of: pF1KB7201.seq Check: 3537 from: 1 to: 5195
pF1KB7201 5195 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AatII G_ACGT'C
Cuts at: 2114 2114
Size: 5195
AccI GT'mk_AC
Cuts at: 1316 2233 1316
Size: 917 4278
Acc65I G'GTAC_C
Cuts at: 2211 2211
Size: 5195
AclI AA'CG_TT
Cuts at: 5035 5035
Size: 5195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3452 4199 4467 3020
Size: 432 747 268 3748
Fragments arranged by size:
3748 747 432 268
AfeI AGC'GCT
Cuts at: 169 1021 169
Size: 852 4343
AflIII A'CryG_T
Cuts at: 2547 3651 4628 2547
Size: 1104 977 3114
Fragments arranged by size:
3114 1104 977
AgeI A'CCGG_T
Cuts at: 3583 3583
Size: 5195
AlwNI CAG_nnn'CTG
Cuts at: 1539 2500 4067 1539
Size: 961 1567 2667
Fragments arranged by size:
2667 1567 961
ApaLI G'TGCA_C
Cuts at: 313 1293 1956 3965 313
Size: 980 663 2009 1543
Fragments arranged by size:
2009 1543 980 663
ApoI r'AATT_y
Cuts at: 2199 4548 4804 2199
Size: 2349 256 2590
Fragments arranged by size:
2590 2349 256
AscI GG'CGCG_CC
Cuts at: 1136 1136
Size: 5195
AseI AT'TA_AT
Cuts at: 20 20
Size: 5195
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
AvaI C'yCGr_G
Cuts at: 523 796 1215 1905 2215 523
Size: 273 419 690 310 3503
Fragments arranged by size:
3503 690 419 310 273
BamHI G'GATC_C
Cuts at: 2220 2220
Size: 5195
BanI G'GyrC_C
Cuts at: 723 800 1566 1594 1984 2008 2211 2625 2873
Size: 77 766 28 390 24 203 414 248
Cuts at: 2873 2908 723
Size: 35 3010
Fragments arranged by size:
3010 766 414 390 248 203 77 35
28 24
BanII G_rGCy'C
Cuts at: 797 2098 2152 2209 3239 797
Size: 1301 54 57 1030 2753
Fragments arranged by size:
2753 1301 1030 57 54
BbeI G_GCGC'C
Cuts at: 727 1570 1988 2012 2629 2877 727
Size: 843 418 24 617 248 3045
Fragments arranged by size:
3045 843 617 418 248 24
BbsI GAAGACnn'nnnn_
Cuts at: 933 1296 1854 933
Size: 363 558 4274
Fragments arranged by size:
4274 558 363
BciVI GTATCCnnnnn_n'
Cuts at: 3093 3854 5195 3093
Size: 761 1341 3093
Fragments arranged by size:
3093 1341 761
BclI T'GATC_A
Cuts at: 2715 2715
Size: 5195
BglI GCCn_nnn'nGGC
Cuts at: 154 193 482 154
Size: 39 289 4867
Fragments arranged by size:
4867 289 39
BglII A'GATC_T
Cuts at: 2710 4747 2710
Size: 2037 3158
BlpI GC'TnA_GC
Cuts at: 1483 2307 1483
Size: 824 4371
Bme1580I G_kGCm'C
Cuts at: 317 1297 1454 1736 1960 2143 2820 2913 3969
Size: 980 157 282 224 183 677 93 1056
Cuts at: 3969 317
Size: 1543
Fragments arranged by size:
1543 1056 980 677 282 224 183 157
93
BmgBI CAC'GTC
Cuts at: 2129 2129
Size: 5195
BmrI ACTGGGnnnn_n'
Cuts at: 2407 2488 2823 4967 2407
Size: 81 335 2144 2635
Fragments arranged by size:
2635 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3562 3562
Size: 5195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1705 1957 328
Size: 1377 252 3566
Fragments arranged by size:
3566 1377 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1855 2371 3250 3742 4040 343
Size: 1512 516 879 492 298 1498
Fragments arranged by size:
1512 1498 879 516 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1267 4899 1267
Size: 3632 1563
BsaAI yAC'GTr
Cuts at: 1263 1524 2548 1263
Size: 261 1024 3910
Fragments arranged by size:
3910 1024 261
BsaBI GATnn'nnATC
Cuts at: 6 2709 4746 6
Size: 2703 2037 455
Fragments arranged by size:
2703 2037 455
BsaHI Gr'CG_yC
Cuts at: 724 1567 1985 2009 2111 2626 2874 3576 5072
Size: 843 418 24 102 515 248 702 1496
Cuts at: 5072 724
Size: 847
Fragments arranged by size:
1496 847 843 702 515 418 248 102
24
BsaWI w'CCGG_w
Cuts at: 2384 2905 3583 3857 4004 4415 2384
Size: 521 678 274 147 411 3164
Fragments arranged by size:
3164 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 328
Size: 30 5165
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1252 1252
Size: 5195
BseYI C'CCAG_C
Cuts at: 589 729 1156 1242 1453 1787 1970 2620 3378
Size: 140 427 86 211 334 183 650 758
Cuts at: 3378 3955 4451 589
Size: 577 496 1333
Fragments arranged by size:
1333 758 650 577 496 427 334 211
183 140 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 1146 393
Size: 753 4442
BsiEI CG_ry'CG
Cuts at: 104 188 501 516 657 1382 2018 2137 2737
Size: 84 313 15 141 725 636 119 600
Cuts at: 2737 2783 3991 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 725 636 600 313 141 119
84 46 15
BsiHKAI G_wGCw'C
Cuts at: 317 349 968 1297 1724 1960 2152 2209 2987
Size: 32 619 329 427 236 192 57 778
Cuts at: 2987 3177 3969 317
Size: 190 792 1543
Fragments arranged by size:
1543 792 778 619 427 329 236 192
190 57 32
BsmBI CGTCTCn'nnnn_
Cuts at: 999 999
Size: 5195
Bsp1286I G_dGCh'C
Cuts at: 317 349 797 968 1297 1454 1724 1736 1960
Size: 32 448 171 329 157 270 12 224
Cuts at: 1960 2098 2143 2152 2209 2820 2913 2987 3177
Size: 138 45 9 57 611 93 74 190
Cuts at: 3177 3239 3969 317
Size: 62 730 1543
Fragments arranged by size:
1543 730 611 448 329 270 224 190
171 157 138 93 74 62 57 45
32 12 9
BspEI T'CCGG_A
Cuts at: 4415 4415
Size: 5195
BspHI T'CATG_A
Cuts at: 4371 4371
Size: 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1556 1832 2245 2459 2761 3142 1556
Size: 276 413 214 302 381 3609
Fragments arranged by size:
3609 413 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 431 468 849 1038 1640 3487 3541 5025
Size: 381 37 381 189 602 1847 54 1484
Cuts at: 5025 5190 50
Size: 165 55
Fragments arranged by size:
1847 1484 602 381 381 189 165 55
54 37
BsrDI GCAATG_nn'
Cuts at: 3107 3107
Size: 5195
BsrFI r'CCGG_y
Cuts at: 454 515 1279 1381 1411 1612 3193 3374 3583
Size: 61 764 102 30 201 1581 181 209
Cuts at: 3583 454
Size: 2066
Fragments arranged by size:
2066 1581 764 209 201 181 102 61
30
BsrGI T'GTAC_A
Cuts at: 380 380
Size: 5195
BssHII G'CGCG_C
Cuts at: 444 461 812 887 927 1136 444
Size: 17 351 75 40 209 4503
Fragments arranged by size:
4503 351 209 75 40 17
BssSI C'ACGA_G
Cuts at: 1111 3466 3824 1111
Size: 2355 358 2482
Fragments arranged by size:
2482 2355 358
BstAPI GCAn_nnn'nTGC
Cuts at: 509 1263 1964 2572 509
Size: 754 701 608 3132
Fragments arranged by size:
3132 754 701 608
BstBI TT'CG_AA
Cuts at: 3556 3556
Size: 5195
BstEII G'GTnAC_C
Cuts at: 118 118
Size: 5195
BstXI CCAn_nnnn'nTGG
Cuts at: 204 204
Size: 5195
BstYI r'GATC_y
Cuts at: 1 338 2220 2687 2710 3045 3291 4303 4389
Size: 337 1882 467 23 335 246 1012 86
Cuts at: 4389 4401 4747 1
Size: 12 346 449
Fragments arranged by size:
1882 1012 467 449 346 337 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 651 684 1298 1739 1988 2012 2018 107
Size: 544 33 614 441 249 24 6 3284
Fragments arranged by size:
3284 614 544 441 249 33 24 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1392 1452 3145 3326 3489 1392
Size: 60 1693 181 163 3098
Fragments arranged by size:
3098 1693 181 163 60
BtsI GCAGTG_nn'
Cuts at: 2438 2438
Size: 5195
DraI TTT'AAA
Cuts at: 2194 2194
Size: 5195
DraIII CAC_nnn'GTG
Cuts at: 548 548
Size: 5195
DrdI GACnn_nn'nnGTC
Cuts at: 1858 1938 2133 2901 3759 1858
Size: 80 195 768 858 3294
Fragments arranged by size:
3294 858 768 195 80
EaeI y'GGCC_r
Cuts at: 146 185 194 325 648 654 1331 1379 1436
Size: 39 9 131 323 6 677 48 57
Cuts at: 1436 1610 2015 2780 2954 3345 3372 4717 146
Size: 174 405 765 174 391 27 1345 624
Fragments arranged by size:
1345 765 677 624 405 391 323 174
174 131 57 48 39 27 9 6
EagI C'GGCC_G
Cuts at: 185 654 1379 2015 2780 185
Size: 469 725 636 765 2600
Fragments arranged by size:
2600 765 725 636 469
EarI CTCTTCn'nnn_
Cuts at: 1075 1591 1633 1765 3218 3428 1075
Size: 516 42 132 1453 210 2842
Fragments arranged by size:
2842 1453 516 210 132 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1690 3403 3713 3859 5005 1690
Size: 1713 310 146 1146 1880
Fragments arranged by size:
1880 1713 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2150 2207 2150
Size: 57 5138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1705 1957 3020 3452 4199 4467 328
Size: 1377 252 1063 432 747 268 1056
Fragments arranged by size:
1377 1063 1056 747 432 268 252
EcoO109I rG'GnC_Cy
Cuts at: 674 1821 2059 2334 674
Size: 1147 238 275 3535
Fragments arranged by size:
3535 1147 275 238
EcoRI G'AATT_C
Cuts at: 2199 2199
Size: 5195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 5195
FspI TGC'GCA
Cuts at: 2976 2976
Size: 5195
HaeII r_GCGC'y
Cuts at: 160 171 182 727 843 1023 1157 1483 1570
Size: 11 11 545 116 180 134 326 87
Cuts at: 1570 1950 1988 2012 2629 2877 3899 4878 160
Size: 380 38 24 617 248 1022 979 477
Fragments arranged by size:
1022 979 617 545 477 380 326 248
180 134 116 87 38 24 11 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3037 3067 3069 3099 3037
Size: 30 2 30 5133
Fragments arranged by size:
5133 30 30 2
HincII GTy'rAC
Cuts at: 2234 2234
Size: 5195
KasI G'GCGC_C
Cuts at: 723 1566 1984 2008 2625 2873 723
Size: 843 418 24 617 248 3045
Fragments arranged by size:
3045 843 617 418 248 24
KpnI G_GTAC'C
Cuts at: 2215 2215
Size: 5195
MluI A'CGCG_T
Cuts at: 4628 4628
Size: 5195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 3866 4050 107
Size: 3759 184 1252
Fragments arranged by size:
3759 1252 184
MscI TGG'CCA
Cuts at: 1333 2956 1333
Size: 1623 3572
MslI CAynn'nnrTG
Cuts at: 262 1471 3184 3311 262
Size: 1209 1713 127 2146
Fragments arranged by size:
2146 1713 1209 127
MspA1I CmG'CkG
Cuts at: 231 330 417 647 686 1130 1572 1741 1964
Size: 99 87 230 39 444 442 169 223
Cuts at: 1964 1990 2020 2306 2620 2980 3993 4238 231
Size: 26 30 286 314 360 1013 245 1188
Fragments arranged by size:
1188 1013 444 442 360 314 286 245
230 223 169 99 87 39 30 26
NaeI GCC'GGC
Cuts at: 456 1413 1614 3376 456
Size: 957 201 1762 2275
Fragments arranged by size:
2275 1762 957 201
NarI GG'CG_CC
Cuts at: 724 1567 1985 2009 2626 2874 724
Size: 843 418 24 617 248 3045
Fragments arranged by size:
3045 843 617 418 248 24
NcoI C'CATG_G
Cuts at: 107 107
Size: 5195
NgoMIV G'CCGG_C
Cuts at: 454 1411 1612 3374 454
Size: 957 201 1762 2275
Fragments arranged by size:
2275 1762 957 201
NotI GC'GGCC_GC
Cuts at: 185 185
Size: 5195
NspI r_CATG'y
Cuts at: 212 267 381 507 2248 2748 3655 212
Size: 55 114 126 1741 500 907 1752
Fragments arranged by size:
1752 1741 907 500 126 114 55
PciI A'CATG_T
Cuts at: 3651 3651
Size: 5195
PflMI CCAn_nnn'nTGG
Cuts at: 113 1346 1539 113
Size: 1233 193 3769
Fragments arranged by size:
3769 1233 193
PmeI GTTT'AAAC
Cuts at: 2194 2194
Size: 5195
PmlI CAC'GTG
Cuts at: 1263 1263
Size: 5195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 4334
Size: 32 5163
PpuMI rG'GwC_Cy
Cuts at: 1821 2059 1821
Size: 238 4957
PstI C_TGCA'G
Cuts at: 167 1829 2242 2927 167
Size: 1662 413 685 2435
Fragments arranged by size:
2435 1662 685 413
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5195
PvuII CAG'CTG
Cuts at: 2620 2980 2620
Size: 360 4835
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 5195
SacI G_AGCT'C
Cuts at: 2152 2209 2152
Size: 57 5138
SacII CC_GC'GG
Cuts at: 687 1742 1991 2021 687
Size: 1055 249 30 3861
Fragments arranged by size:
3861 1055 249 30
SalI G'TCGA_C
Cuts at: 2232 2232
Size: 5195
SanDI GG'GwC_CC
Cuts at: 2059 2059
Size: 5195
SapI GCTCTTCn'nnn_
Cuts at: 3218 3428 3218
Size: 210 4985
SbfI CC_TGCA'GG
Cuts at: 2242 2242
Size: 5195
ScaI AGT'ACT
Cuts at: 1426 1876 4712 1426
Size: 450 2836 1909
Fragments arranged by size:
2836 1909 450
SfcI C'TryA_G
Cuts at: 33 163 1825 2103 2238 2923 3916 4107 33
Size: 130 1662 278 135 685 993 191 1121
Fragments arranged by size:
1662 1121 993 685 278 191 135 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 193 193
Size: 5195
SfoI GGC'GCC
Cuts at: 725 1568 1986 2010 2627 2875 725
Size: 843 418 24 617 248 3045
Fragments arranged by size:
3045 843 617 418 248 24
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5195
SmaI CCC'GGG
Cuts at: 798 1217 2217 798
Size: 419 1000 3776
Fragments arranged by size:
3776 1000 419
SmlI C'TyrA_G
Cuts at: 358 1870 2350 3265 3757 4019 358
Size: 1512 480 915 492 262 1534
Fragments arranged by size:
1534 1512 915 492 480 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5195
SphI G_CATG'C
Cuts at: 212 507 2248 2748 212
Size: 295 1741 500 2659
Fragments arranged by size:
2659 1741 500 295
SrfI GCCC'GGGC
Cuts at: 1217 1217
Size: 5195
StyI C'CwwG_G
Cuts at: 107 152 197 449 2329 2683 107
Size: 45 45 252 1880 354 2619
Fragments arranged by size:
2619 1880 354 252 45 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1632 2778 2911 3370 3579 1338
Size: 294 1146 133 459 209 2954
Fragments arranged by size:
2954 1146 459 294 209 133
TatI w'GTAC_w
Cuts at: 380 1424 1874 4710 380
Size: 1044 450 2836 865
Fragments arranged by size:
2836 1044 865 450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 4712 4744 328
Size: 32 4352 32 779
Fragments arranged by size:
4352 779 32 32
Tth111I GACn'n_nGTC
Cuts at: 2992 2992
Size: 5195
XbaI T'CTAG_A
Cuts at: 2226 2226
Size: 5195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1473 1605 2690 1473
Size: 132 1085 3978
Fragments arranged by size:
3978 1085 132
XmaI C'CCGG_G
Cuts at: 796 1215 2215 796
Size: 419 1000 3776
Fragments arranged by size:
3776 1000 419
ZraI GAC'GTC
Cuts at: 2112 2112
Size: 5195
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PasI PfoI PshAI PsiI PspOMI PspXI PsrI SexAI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI