Restriction map for
pF1KB7204
(Circular) (Six-base) MAPSORT of: pF1KB7204.seq Check: 9394 from: 1 to: 3680
pF1KB7204 3680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 363 1505 1937 2684 2952 286
Size: 77 1142 432 747 268 1014
Fragments arranged by size:
1142 1014 747 432 268 77
AflIII A'CryG_T
Cuts at: 1032 2136 3113 1032
Size: 1104 977 1599
Fragments arranged by size:
1599 1104 977
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 453 985 2552 453
Size: 532 1567 1581
Fragments arranged by size:
1581 1567 532
ApaLI G'TGCA_C
Cuts at: 2450 2450
Size: 3680
ApoI r'AATT_y
Cuts at: 427 684 3033 3289 427
Size: 257 2349 256 818
Fragments arranged by size:
2349 818 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 700 700
Size: 3680
BamHI G'GATC_C
Cuts at: 705 705
Size: 3680
BanI G'GyrC_C
Cuts at: 696 1110 1358 1393 696
Size: 414 248 35 2983
Fragments arranged by size:
2983 414 248 35
BanII G_rGCy'C
Cuts at: 694 1724 694
Size: 1030 2650
BbeI G_GCGC'C
Cuts at: 1114 1362 1114
Size: 248 3432
BciVI GTATCCnnnnn_n'
Cuts at: 1578 2339 3680 1578
Size: 761 1341 1578
Fragments arranged by size:
1578 1341 761
BclI T'GATC_A
Cuts at: 1200 1200
Size: 3680
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 148 792 148
Size: 644 3036
Bme1580I G_kGCm'C
Cuts at: 1305 1398 2454 1305
Size: 93 1056 2531
Fragments arranged by size:
2531 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 892 973 1308 3452 892
Size: 81 335 2144 1120
Fragments arranged by size:
2144 1120 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 3680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 856 1735 2227 2525 314
Size: 542 879 492 298 1469
Fragments arranged by size:
1469 879 542 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 406 3384 406
Size: 2978 702
BsaAI yAC'GTr
Cuts at: 1033 1033
Size: 3680
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 1111 1359 2061 3557 1111
Size: 248 702 1496 1234
Fragments arranged by size:
1496 1234 702 248
BsaWI w'CCGG_w
Cuts at: 869 1390 2068 2342 2489 2900 869
Size: 521 678 274 147 411 1649
Fragments arranged by size:
1649 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1105 1863 2440 2936 1105
Size: 758 577 496 1849
Fragments arranged by size:
1849 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1222 1268 2476 104
Size: 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 46
BsiHKAI G_wGCw'C
Cuts at: 149 694 1472 1662 2454 149
Size: 545 778 190 792 1375
Fragments arranged by size:
1375 792 778 545 190
Bsp1286I G_dGCh'C
Cuts at: 149 694 1305 1398 1472 1662 1724 2454 149
Size: 545 611 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 545 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 620 2856 620
Size: 2236 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 944 1246 1627 730
Size: 214 302 381 2783
Fragments arranged by size:
2783 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1972 2026 3510 3675 50
Size: 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 1592
Size: 3680
BsrFI r'CCGG_y
Cuts at: 1678 1859 2068 1678
Size: 181 209 3290
Fragments arranged by size:
3290 209 181
BssSI C'ACGA_G
Cuts at: 1951 2309 1951
Size: 358 3322
BstAPI GCAn_nnn'nTGC
Cuts at: 1057 1057
Size: 3680
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstYI r'GATC_y
Cuts at: 1 625 705 1172 1195 1530 1776 2788 2874
Size: 624 80 467 23 335 246 1012 86
Cuts at: 2874 2886 3232 1
Size: 12 346 449
Fragments arranged by size:
1012 624 467 449 346 335 246 86
80 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 1811 1974 1630
Size: 181 163 3336
Fragments arranged by size:
3336 181 163
BtsI GCAGTG_nn'
Cuts at: 923 923
Size: 3680
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 1386 2244 1386
Size: 858 2822
EaeI y'GGCC_r
Cuts at: 134 1265 1439 1830 1857 3202 134
Size: 1131 174 391 27 1345 612
Fragments arranged by size:
1345 1131 612 391 174 27
EagI C'GGCC_G
Cuts at: 1265 1265
Size: 3680
EarI CTCTTCn'nnn_
Cuts at: 382 491 1703 1913 382
Size: 109 1212 210 2149
Fragments arranged by size:
2149 1212 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1888 2198 2344 3490 1888
Size: 310 146 1146 2078
Fragments arranged by size:
2078 1146 310 146
EcoICRI GAG'CTC
Cuts at: 692 692
Size: 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 363 1505 1937 2684 2952 286
Size: 77 1142 432 747 268 1014
Fragments arranged by size:
1142 1014 747 432 268 77
EcoO109I rG'GnC_Cy
Cuts at: 215 819 215
Size: 604 3076
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FspI TGC'GCA
Cuts at: 1461 1461
Size: 3680
HaeII r_GCGC'y
Cuts at: 1114 1362 2384 3363 1114
Size: 248 1022 979 1431
Fragments arranged by size:
1431 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 602 634 1522 1552 1554 1584 357
Size: 32 213 32 888 30 2 30 2453
Fragments arranged by size:
2453 888 213 32 32 30 30 2
HincII GTy'rAC
Cuts at: 719 719
Size: 3680
KasI G'GCGC_C
Cuts at: 1110 1358 1110
Size: 248 3432
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2535 2351
Size: 184 3496
MscI TGG'CCA
Cuts at: 1441 1441
Size: 3680
MslI CAynn'nnrTG
Cuts at: 1669 1796 1669
Size: 127 3553
MspA1I CmG'CkG
Cuts at: 791 1105 1465 2478 2723 791
Size: 314 360 1013 245 1748
Fragments arranged by size:
1748 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1861 1861
Size: 3680
NarI GG'CG_CC
Cuts at: 1111 1359 1111
Size: 248 3432
NcoI C'CATG_G
Cuts at: 107 107
Size: 3680
NgoMIV G'CCGG_C
Cuts at: 1859 1859
Size: 3680
NspI r_CATG'y
Cuts at: 733 1233 2140 733
Size: 500 907 2273
Fragments arranged by size:
2273 907 500
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PstI C_TGCA'G
Cuts at: 727 1412 727
Size: 685 2995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 1105 1465 1105
Size: 360 3320
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 694 694
Size: 3680
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 723 1408 2401 2592 33
Size: 690 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 191
SfoI GGC'GCC
Cuts at: 1112 1360 1112
Size: 248 3432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SmaI CCC'GGG
Cuts at: 702 702
Size: 3680
SmlI C'TyrA_G
Cuts at: 329 835 1750 2242 2504 329
Size: 506 915 492 262 1505
Fragments arranged by size:
1505 915 506 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
StyI C'CwwG_G
Cuts at: 107 814 1168 107
Size: 707 354 2619
Fragments arranged by size:
2619 707 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 3195 3195
Size: 3680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 3648
Tth111I GACn'n_nGTC
Cuts at: 1477 1477
Size: 3680
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 1175 168
Size: 1007 2673
XmaI C'CCGG_G
Cuts at: 700 700
Size: 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI