Restriction map for
pF1KB7209
(Circular) (Six-base) MAPSORT of: pF1KB7209.seq Check: 5875 from: 1 to: 3716
pF1KB7209 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 656 688 656
Size: 32 3684
AlwNI CAG_nnn'CTG
Cuts at: 525 1021 2588 525
Size: 496 1567 1653
Fragments arranged by size:
1653 1567 496
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 451 464 730 1760 451
Size: 13 266 1030 2407
Fragments arranged by size:
2407 1030 266 13
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbsI GAAGACnn'nnnn_
Cuts at: 215 215
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 278 1236 278
Size: 958 2758
BfrBI ATG'CAT
Cuts at: 227 227
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 626 828 626
Size: 202 3514
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2490 1341
Size: 93 1056 2567
Fragments arranged by size:
2567 1056 93
BmgBI CAC'GTC
Cuts at: 188 188
Size: 3716
BmrI ACTGGGnnnn_n'
Cuts at: 390 928 1009 1344 3488 390
Size: 538 81 335 2144 618
Fragments arranged by size:
2144 618 538 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 1069 1069
Size: 3716
BsaBI GATnn'nnATC
Cuts at: 6 283 1230 3267 6
Size: 277 947 2037 455
Fragments arranged by size:
2037 947 455 277
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 182 905 1426 2104 2378 2525 2936 182
Size: 723 521 678 274 147 411 962
Fragments arranged by size:
962 723 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 559 1141 1899 2476 2972 559
Size: 582 758 577 496 1303
Fragments arranged by size:
1303 758 582 577 496
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 451 730 1508 1698 2490 451
Size: 279 778 190 792 1677
Fragments arranged by size:
1677 792 778 279 190
Bsp1286I G_dGCh'C
Cuts at: 451 464 730 1341 1434 1508 1698 1760 2490
Size: 13 266 611 93 74 190 62 730
Cuts at: 2490 451
Size: 1677
Fragments arranged by size:
1677 730 611 266 190 93 74 62
13
BspEI T'CCGG_A
Cuts at: 182 2936 182
Size: 2754 962
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 741 1208 1231 1566 1812 2824 2910 2922
Size: 740 467 23 335 246 1012 86 12
Cuts at: 2922 3268 1
Size: 346 449
Fragments arranged by size:
1012 740 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 587 587
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 589 1422 2280 589
Size: 833 858 2025
Fragments arranged by size:
2025 858 833
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3238 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 728 449
Size: 279 3437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1541 1973 2720 2988 427
Size: 1114 432 747 268 1155
Fragments arranged by size:
1155 1114 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 218 218
Size: 3716
EcoO109I rG'GnC_Cy
Cuts at: 473 522 855 473
Size: 49 333 3334
Fragments arranged by size:
3334 333 49
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 1735 324
Size: 1411 2305
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 1150 1398 2420 3399 1150
Size: 248 1022 979 1467
Fragments arranged by size:
1467 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 267 1705 1832 267
Size: 1438 127 2151
Fragments arranged by size:
2151 1438 127
MspA1I CmG'CkG
Cuts at: 347 827 1141 1501 2514 2759 347
Size: 480 314 360 1013 245 1304
Fragments arranged by size:
1304 1013 480 360 314 245
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 587 587
Size: 3716
NdeI CA'TA_TG
Cuts at: 119 119
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NsiI A_TGCA'T
Cuts at: 229 229
Size: 3716
NspI r_CATG'y
Cuts at: 642 769 1269 2176 642
Size: 127 500 907 2182
Fragments arranged by size:
2182 907 500 127
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 149 181 149
Size: 32 3684
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3031
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 451 730 451
Size: 279 3437
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 382 3233 382
Size: 2851 865
SfcI C'TryA_G
Cuts at: 33 218 759 1444 2437 2628 33
Size: 185 541 685 993 191 1121
Fragments arranged by size:
1121 993 685 541 191 185
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 587 850 1204 587
Size: 263 354 3099
Fragments arranged by size:
3099 354 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 332 380 641 3231 332
Size: 48 261 2590 817
Fragments arranged by size:
2590 817 261 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI