Restriction map for
pF1KB7214
(Circular) (Six-base) MAPSORT of: pF1KB7214.seq Check: 372 from: 1 to: 3992
pF1KB7214 3992 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 378 378
Size: 3992
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 290 748 1817 2249 2996 3264 142
Size: 148 458 1069 432 747 268 870
Fragments arranged by size:
1069 870 747 458 432 268 148
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AleI CACnn'nnGTG
Cuts at: 321 448 535 321
Size: 127 87 3778
Fragments arranged by size:
3778 127 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 483 483
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 268 707 1297 2864 268
Size: 439 590 1567 1396
Fragments arranged by size:
1567 1396 590 439
ApaI G_GGCC'C
Cuts at: 280 280
Size: 3992
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 636 678 758 996 3345 3601 636
Size: 42 80 238 2349 256 1027
Fragments arranged by size:
2349 1027 256 238 80 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 128 169 1012 128
Size: 41 843 3108
Fragments arranged by size:
3108 843 41
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 195 427 1008 1422 1670 1705 195
Size: 232 581 414 248 35 2482
Fragments arranged by size:
2482 581 414 248 232 35
BanII G_rGCy'C
Cuts at: 256 280 1006 2036 256
Size: 24 726 1030 2212
Fragments arranged by size:
2212 1030 726 24
BbeI G_GCGC'C
Cuts at: 199 1426 1674 199
Size: 1227 248 2517
Fragments arranged by size:
2517 1227 248
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 280 1617 1710 2766 280
Size: 1337 93 1056 1506
Fragments arranged by size:
1506 1337 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 189 1204 1285 1620 3764 189
Size: 1015 81 335 2144 417
Fragments arranged by size:
2144 1015 417 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 275 2359 275
Size: 2084 1908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 601 245
Size: 356 3636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 549 3696 549
Size: 3147 845
BsaAI yAC'GTr
Cuts at: 446 1345 446
Size: 899 3093
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 196 1423 1671 2373 3869 196
Size: 1227 248 702 1496 319
Fragments arranged by size:
1496 1227 702 319 248
BsaWI w'CCGG_w
Cuts at: 715 1181 1702 2380 2654 2801 3212 715
Size: 466 521 678 274 147 411 1495
Fragments arranged by size:
1495 678 521 466 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 241 368 208
Size: 33 127 3832
Fragments arranged by size:
3832 127 33
BseYI C'CCAG_C
Cuts at: 707 965 1417 2175 2752 3248 707
Size: 258 452 758 577 496 1451
Fragments arranged by size:
1451 758 577 496 452 258
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1784 1974 2766 1006
Size: 778 190 792 2232
Fragments arranged by size:
2232 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 256 280 1006 1617 1710 1784 1974 2036 2766
Size: 24 726 611 93 74 190 62 730
Cuts at: 2766 256
Size: 1482
Fragments arranged by size:
1482 730 726 611 190 93 74 62
24
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 3168 3168
Size: 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 378 1042 1256 1558 1939 378
Size: 664 214 302 381 2431
Fragments arranged by size:
2431 664 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 597 2353 597
Size: 1756 2236
BstEII G'GTnAC_C
Cuts at: 435 711 435
Size: 276 3716
BstYI r'GATC_y
Cuts at: 1 1017 1484 1507 1842 2088 3100 3186 3198
Size: 1016 467 23 335 246 1012 86 12
Cuts at: 3198 3544 1
Size: 346 449
Fragments arranged by size:
1016 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 671 671
Size: 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 316 465 1235 316
Size: 149 770 3073
Fragments arranged by size:
3073 770 149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 521 556 521
Size: 35 3957
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 943 1698 2556 943
Size: 755 858 2379
Fragments arranged by size:
2379 858 755
EaeI y'GGCC_r
Cuts at: 327 1577 1751 2142 2169 3514 327
Size: 1250 174 391 27 1345 805
Fragments arranged by size:
1345 1250 805 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 245 290 601 748 1817 2249 2996 3264
Size: 103 45 311 147 1069 432 747 268
Cuts at: 3264 142
Size: 870
Fragments arranged by size:
1069 870 747 432 311 268 147 103
45
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 3992
EcoO109I rG'GnC_Cy
Cuts at: 132 265 277 1131 132
Size: 133 12 854 2993
Fragments arranged by size:
2993 854 133 12
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 199 1426 1674 2696 3675 199
Size: 1227 248 1022 979 516
Fragments arranged by size:
1227 1022 979 516 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 492 524 548 580 1834 1864 1866 1896 492
Size: 32 24 32 1254 30 2 30 2588
Fragments arranged by size:
2588 1254 32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
KasI G'GCGC_C
Cuts at: 195 1422 1670 195
Size: 1227 248 2517
Fragments arranged by size:
2517 1227 248
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 648 2663 2847 648
Size: 2015 184 1793
Fragments arranged by size:
2015 1793 184
MscI TGG'CCA
Cuts at: 1753 1753
Size: 3992
MslI CAynn'nnrTG
Cuts at: 321 448 491 535 1981 2108 321
Size: 127 43 44 1446 127 2205
Fragments arranged by size:
2205 1446 127 127 44 43
MspA1I CmG'CkG
Cuts at: 1103 1417 1777 2790 3035 1103
Size: 314 360 1013 245 2060
Fragments arranged by size:
2060 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 196 1423 1671 196
Size: 1227 248 2517
Fragments arranged by size:
2517 1227 248
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PflMI CCAn_nnn'nTGG
Cuts at: 707 707
Size: 3992
PfoI T'CCnGG_A
Cuts at: 261 261
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PmlI CAC'GTG
Cuts at: 446 446
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PpuMI rG'GwC_Cy
Cuts at: 265 265
Size: 3992
PspOMI G'GGCC_C
Cuts at: 276 276
Size: 3992
PstI C_TGCA'G
Cuts at: 1039 1724 1039
Size: 685 3307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 1417 1777 1417
Size: 360 3632
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SexAI A'CCwGG_T
Cuts at: 700 700
Size: 3992
SfcI C'TryA_G
Cuts at: 33 1035 1720 2713 2904 33
Size: 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 191
SfoI GGC'GCC
Cuts at: 197 1424 1672 197
Size: 1227 248 2517
Fragments arranged by size:
2517 1227 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 130 171 1014 130
Size: 41 843 3108
Fragments arranged by size:
3108 843 41
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
SrfI GCCC'GGGC
Cuts at: 171 171
Size: 3992
StuI AGG'CCT
Cuts at: 823 823
Size: 3992
StyI C'CwwG_G
Cuts at: 1126 1480 1126
Size: 354 3638
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 438 699 1575 1708 2167 2376 438
Size: 261 876 133 459 209 2054
Fragments arranged by size:
2054 876 459 261 209 133
TatI w'GTAC_w
Cuts at: 3507 3507
Size: 3992
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 361 393 457 489 849 881 3509 3541 361
Size: 32 64 32 360 32 2628 32 812
Fragments arranged by size:
2628 812 360 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 128 169 1012 128
Size: 41 843 3108
Fragments arranged by size:
3108 843 41
XmnI GAAnn'nnTTC
Cuts at: 761 888 761
Size: 127 3865
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I BtgI ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SspI SwaI XhoI ZraI