Restriction map for
pF1KB7226
(Circular) (Six-base) MAPSORT of: pF1KB7226.seq Check: 9797 from: 1 to: 4472
pF1KB7226 4472 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4472
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 1025 1213 2297 2729 3476 3744 756
Size: 269 188 1084 432 747 268 1484
Fragments arranged by size:
1484 1084 747 432 269 268 188
AflIII A'CryG_T
Cuts at: 1824 2928 3905 1824
Size: 1104 977 2391
Fragments arranged by size:
2391 1104 977
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 876 1777 3344 876
Size: 901 1567 2004
Fragments arranged by size:
2004 1567 901
ApaLI G'TGCA_C
Cuts at: 1180 1210 3242 1180
Size: 30 2032 2410
Fragments arranged by size:
2410 2032 30
ApoI r'AATT_y
Cuts at: 1476 3825 4081 1476
Size: 2349 256 1867
Fragments arranged by size:
2349 1867 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 1492 1492
Size: 4472
BamHI G'GATC_C
Cuts at: 116 1497 116
Size: 1381 3091
BanI G'GyrC_C
Cuts at: 709 1488 1902 2150 2185 709
Size: 779 414 248 35 2996
Fragments arranged by size:
2996 779 414 248 35
BanII G_rGCy'C
Cuts at: 1379 1486 2516 1379
Size: 107 1030 3335
Fragments arranged by size:
3335 1030 107
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 547 1090 547
Size: 543 3929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4438
BciVI GTATCCnnnnn_n'
Cuts at: 939 2370 3131 4472 939
Size: 1431 761 1341 939
Fragments arranged by size:
1431 1341 939 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BglI GCCn_nnn'nGGC
Cuts at: 707 829 707
Size: 122 4350
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1418 1584 1418
Size: 166 4306
Bme1580I G_kGCm'C
Cuts at: 174 712 1184 1214 2097 2190 3246 174
Size: 538 472 30 883 93 1056 1400
Fragments arranged by size:
1400 1056 883 538 472 93 30
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1684 1765 2100 4244 1342
Size: 342 81 335 2144 1570
Fragments arranged by size:
2144 1570 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1169 2839 1169
Size: 1670 2802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 377 1141 259
Size: 118 764 3590
Fragments arranged by size:
3590 764 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1648 2527 3019 3317 1648
Size: 879 492 298 2803
Fragments arranged by size:
2803 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 1903 2151 2853 4349 1903
Size: 248 702 1496 2026
Fragments arranged by size:
2026 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 496
Size: 4472
BseYI C'CCAG_C
Cuts at: 882 1461 1897 2655 3232 3728 882
Size: 579 436 758 577 496 1626
Fragments arranged by size:
1626 758 579 577 496 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 805 1087 586
Size: 219 282 3971
Fragments arranged by size:
3971 282 219
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1184 1214 1486 2264 2454 3246 1184
Size: 30 272 778 190 792 2410
Fragments arranged by size:
2410 792 778 272 190 30
BsmI GAATG_Cn'
Cuts at: 1443 1443
Size: 4472
Bsp1286I G_dGCh'C
Cuts at: 174 712 1184 1214 1379 1486 2097 2190 2264
Size: 538 472 30 165 107 611 93 74
Cuts at: 2264 2454 2516 3246 174
Size: 190 62 730 1400
Fragments arranged by size:
1400 730 611 538 472 190 165 107
93 74 62 30
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 197 3648 197
Size: 3451 1021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 1522 1736 2038 2419 557
Size: 965 214 302 381 2610
Fragments arranged by size:
2610 965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 464 2470 2651 2860 464
Size: 2006 181 209 2076
Fragments arranged by size:
2076 2006 209 181
BssSI C'ACGA_G
Cuts at: 1213 2743 3101 1213
Size: 1530 358 2584
Fragments arranged by size:
2584 1530 358
BstAPI GCAn_nnn'nTGC
Cuts at: 876 907 1849 876
Size: 31 942 3499
Fragments arranged by size:
3499 942 31
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstXI CCAn_nnnn'nTGG
Cuts at: 696 987 1242 696
Size: 291 255 3926
Fragments arranged by size:
3926 291 255
BstYI r'GATC_y
Cuts at: 1 116 1497 1964 1987 2322 2568 3580 3666
Size: 115 1381 467 23 335 246 1012 86
Cuts at: 3666 3678 4024 1
Size: 12 346 449
Fragments arranged by size:
1381 1012 467 449 346 335 246 115
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 845 845
Size: 4472
BtgI C'CryG_G
Cuts at: 218 218
Size: 4472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4128
Fragments arranged by size:
4128 181 163
BtsI GCAGTG_nn'
Cuts at: 172 1715 172
Size: 1543 2929
ClaI AT'CG_AT
Cuts at: 638 1279 638
Size: 641 3831
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 1232 1397 2057 2231 2622 2649 3994 1232
Size: 165 660 174 391 27 1345 1710
Fragments arranged by size:
1710 1345 660 391 174 165 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1158 2680 2990 3136 4282 1158
Size: 1522 310 146 1146 1348
Fragments arranged by size:
1522 1348 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 377 756 1025 1141 1213 2297 2729 3476
Size: 118 379 269 116 72 1084 432 747
Cuts at: 3476 3744 259
Size: 268 987
Fragments arranged by size:
1084 987 747 432 379 269 268 118
116 72
EcoO109I rG'GnC_Cy
Cuts at: 831 1611 831
Size: 780 3692
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4472
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 218 1906 2154 3176 4155 218
Size: 1688 248 1022 979 535
Fragments arranged by size:
1688 1022 979 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 350 382 975 1007 2314 2344 2346 2376 350
Size: 32 593 32 1307 30 2 30 2446
Fragments arranged by size:
2446 1307 593 32 32 30 30 2
HincII GTy'rAC
Cuts at: 579 1511 579
Size: 932 3540
HindIII A'AGCT_T
Cuts at: 811 811
Size: 4472
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 786 3143 3327 786
Size: 2357 184 1931
Fragments arranged by size:
2357 1931 184
MscI TGG'CCA
Cuts at: 1234 1399 2233 1234
Size: 165 834 3473
Fragments arranged by size:
3473 834 165
MslI CAynn'nnrTG
Cuts at: 388 694 2461 2588 388
Size: 306 1767 127 2272
Fragments arranged by size:
2272 1767 306 127
MspA1I CmG'CkG
Cuts at: 327 873 1224 1583 1897 2257 3270 3515 327
Size: 546 351 359 314 360 1013 245 1284
Fragments arranged by size:
1284 1013 546 360 359 351 314 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NcoI C'CATG_G
Cuts at: 218 218
Size: 4472
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NspI r_CATG'y
Cuts at: 564 1525 2025 2932 564
Size: 961 500 907 2104
Fragments arranged by size:
2104 961 907 500
PasI CC'CwG_GG
Cuts at: 166 616 166
Size: 450 4022
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PfoI T'CCnGG_A
Cuts at: 236 587 941 1264 1385 236
Size: 351 354 323 121 3323
Fragments arranged by size:
3323 354 351 323 121
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PshAI GACnn'nnGTC
Cuts at: 773 773
Size: 4472
PstI C_TGCA'G
Cuts at: 139 903 920 1229 1519 2204 139
Size: 764 17 309 290 685 2407
Fragments arranged by size:
2407 764 685 309 290 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 327 873 1224 1897 2257 327
Size: 546 351 673 360 2542
Fragments arranged by size:
2542 673 546 360 351
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 1204 3989 1204
Size: 2785 1687
SexAI A'CCwGG_T
Cuts at: 203 203
Size: 4472
SfcI C'TryA_G
Cuts at: 33 135 899 916 1013 1225 1515 2200 3193
Size: 102 764 17 97 212 290 685 993
Cuts at: 3193 3384 33
Size: 191 1121
Fragments arranged by size:
1121 993 764 685 290 212 191 102
97 17
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 1627 2542 3034 3296 1627
Size: 915 492 262 2803
Fragments arranged by size:
2803 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
StuI AGG'CCT
Cuts at: 491 491
Size: 4472
StyI C'CwwG_G
Cuts at: 218 1070 1606 1960 218
Size: 852 536 354 2730
Fragments arranged by size:
2730 852 536 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2055 2188 2647 2856 2055
Size: 133 459 209 3671
Fragments arranged by size:
3671 459 209 133
TatI w'GTAC_w
Cuts at: 1202 3987 1202
Size: 2785 1687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4440
Tth111I GACn'n_nGTC
Cuts at: 2269 2269
Size: 4472
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 1967 984
Size: 983 3489
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI