Restriction map for
pF1KB7227
(Circular) (Six-base) MAPSORT of: pF1KB7227.seq Check: 681 from: 1 to: 3773
pF1KB7227 3773 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 811 811
Size: 3773
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
AfeI AGC'GCT
Cuts at: 463 463
Size: 3773
AflIII A'CryG_T
Cuts at: 1125 2229 3206 1125
Size: 1104 977 1692
Fragments arranged by size:
1692 1104 977
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 1078 2645 1078
Size: 1567 2206
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 232 777 3126 3382 232
Size: 545 2349 256 623
Fragments arranged by size:
2349 623 545 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 431 793 431
Size: 362 3411
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 789 1203 1451 1486 789
Size: 414 248 35 3076
Fragments arranged by size:
3076 414 248 35
BanII G_rGCy'C
Cuts at: 421 787 1817 421
Size: 366 1030 2377
Fragments arranged by size:
2377 1030 366
BbeI G_GCGC'C
Cuts at: 1207 1455 1207
Size: 248 3525
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglII A'GATC_T
Cuts at: 1288 3325 1288
Size: 2037 1736
BlpI GC'TnA_GC
Cuts at: 885 885
Size: 3773
Bme1580I G_kGCm'C
Cuts at: 1398 1491 2547 1398
Size: 93 1056 2624
Fragments arranged by size:
2624 1056 93
BmgBI CAC'GTC
Cuts at: 742 742
Size: 3773
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 1204 1452 2154 3650 1204
Size: 248 702 1496 1327
Fragments arranged by size:
1496 1327 702 248
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 591 1198 1956 2533 3029 591
Size: 607 758 577 496 1335
Fragments arranged by size:
1335 758 607 577 496
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 1565 1755 2547 787
Size: 778 190 792 2013
Fragments arranged by size:
2013 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 421 787 1398 1491 1565 1755 1817 2547 421
Size: 366 611 93 74 190 62 730 1647
Fragments arranged by size:
1647 730 611 366 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 823 1037 1339 1720 823
Size: 214 302 381 2876
Fragments arranged by size:
2876 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 337 1685 337
Size: 1348 2425
BsrFI r'CCGG_y
Cuts at: 1771 1952 2161 1771
Size: 181 209 3383
Fragments arranged by size:
3383 209 181
BsrGI T'GTAC_A
Cuts at: 452 452
Size: 3773
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstXI CCAn_nnnn'nTGG
Cuts at: 559 559
Size: 3773
BstYI r'GATC_y
Cuts at: 1 798 1265 1288 1623 1869 2881 2967 2979
Size: 797 467 23 335 246 1012 86 12
Cuts at: 2979 3325 1
Size: 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 237 413 540 107
Size: 130 176 127 3340
Fragments arranged by size:
3340 176 130 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1016
Size: 3773
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 1479 2337 1479
Size: 858 2915
EaeI y'GGCC_r
Cuts at: 1358 1532 1923 1950 3295 1358
Size: 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 2291 2437 3583 1981
Size: 310 146 1146 2171
Fragments arranged by size:
2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1598 2030 2777 3045 332
Size: 1266 432 747 268 1060
Fragments arranged by size:
1266 1060 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 912 912
Size: 3773
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 465 1207 1455 2477 3456 465
Size: 742 248 1022 979 782
Fragments arranged by size:
1022 979 782 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 425 457 1615 1645 1647 1677 425
Size: 32 1158 30 2 30 2521
Fragments arranged by size:
2521 1158 32 30 30 2
HincII GTy'rAC
Cuts at: 812 812
Size: 3773
KasI G'GCGC_C
Cuts at: 1203 1451 1203
Size: 248 3525
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MfeI C'AATT_G
Cuts at: 122 122
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2444 2628 701
Size: 1743 184 1846
Fragments arranged by size:
1846 1743 184
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 384 557 1762 1889 384
Size: 173 1205 127 2268
Fragments arranged by size:
2268 1205 173 127
MspA1I CmG'CkG
Cuts at: 542 884 1198 1558 2571 2816 542
Size: 342 314 360 1013 245 1499
Fragments arranged by size:
1499 1013 360 342 314 245
NaeI GCC'GGC
Cuts at: 1954 1954
Size: 3773
NarI GG'CG_CC
Cuts at: 1204 1452 1204
Size: 248 3525
NcoI C'CATG_G
Cuts at: 107 237 107
Size: 130 3643
NdeI CA'TA_TG
Cuts at: 385 385
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 1952 1952
Size: 3773
NspI r_CATG'y
Cuts at: 826 1326 2233 826
Size: 500 907 2366
Fragments arranged by size:
2366 907 500
PciI A'CATG_T
Cuts at: 2229 2229
Size: 3773
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PsiI TTA'TAA
Cuts at: 158 548 158
Size: 390 3383
PstI C_TGCA'G
Cuts at: 820 1505 820
Size: 685 3088
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SacII CC_GC'GG
Cuts at: 543 543
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 816 1501 2494 2685 33
Size: 783 685 993 191 1121
Fragments arranged by size:
1121 993 783 685 191
SfoI GGC'GCC
Cuts at: 1205 1453 1205
Size: 248 3525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 431 928 1843 2335 2597 431
Size: 497 915 492 262 1607
Fragments arranged by size:
1607 915 497 492 262
SpeI A'CTAG_T
Cuts at: 67 606 67
Size: 539 3234
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StyI C'CwwG_G
Cuts at: 107 237 907 1261 107
Size: 130 670 354 2619
Fragments arranged by size:
2619 670 354 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1356 1489 1948 2157 1356
Size: 133 459 209 2972
Fragments arranged by size:
2972 459 209 133
TatI w'GTAC_w
Cuts at: 452 3288 452
Size: 2836 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 226 804 226
Size: 578 3195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XhoI C'TCGA_G
Cuts at: 431 431
Size: 3773
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI