Restriction map for
pF1KB7228
(Circular) (Six-base) MAPSORT of: pF1KB7228.seq Check: 7051 from: 1 to: 4898
pF1KB7228 4898 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1936 1936
Size: 4898
Acc65I G'GTAC_C
Cuts at: 880 1914 880
Size: 1034 3864
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1000 2723 3155 3902 4170 102
Size: 898 1723 432 747 268 830
Fragments arranged by size:
1723 898 830 747 432 268
AflIII A'CryG_T
Cuts at: 814 826 2250 3354 4331 814
Size: 12 1424 1104 977 1381
Fragments arranged by size:
1424 1381 1104 977 12
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AhdI GACnn_n'nnGTC
Cuts at: 1774 1774
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 2203 3770 2203
Size: 1567 3331
ApaLI G'TGCA_C
Cuts at: 3668 3668
Size: 4898
ApoI r'AATT_y
Cuts at: 112 414 740 1384 1902 4251 4507 112
Size: 302 326 644 518 2349 256 503
Fragments arranged by size:
2349 644 518 503 326 302 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 1918 1918
Size: 4898
AvrII C'CTAG_G
Cuts at: 1029 1029
Size: 4898
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 880 1914 2328 2576 2611 880
Size: 1034 414 248 35 3167
Fragments arranged by size:
3167 1034 414 248 35
BanII G_rGCy'C
Cuts at: 1866 1912 2942 1866
Size: 46 1030 3822
Fragments arranged by size:
3822 1030 46
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbvCI CC'TCA_GC
Cuts at: 1097 1097
Size: 4898
BciVI GTATCCnnnnn_n'
Cuts at: 712 2796 3557 4898 712
Size: 2084 761 1341 712
Fragments arranged by size:
2084 1341 761 712
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4898
BglII A'GATC_T
Cuts at: 2413 4450 2413
Size: 2037 2861
BlpI GC'TnA_GC
Cuts at: 520 2010 520
Size: 1490 3408
Bme1580I G_kGCm'C
Cuts at: 2523 2616 3672 2523
Size: 93 1056 3749
Fragments arranged by size:
3749 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2110 2191 2526 4670 2110
Size: 81 335 2144 2338
Fragments arranged by size:
2338 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 449 3265 449
Size: 2816 2082
Bpu10I CC'TnA_GC
Cuts at: 1097 1097
Size: 4898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3445 3743 2074
Size: 879 492 298 3229
Fragments arranged by size:
3229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1193 1876 4602 1193
Size: 683 2726 1489
Fragments arranged by size:
2726 1489 683
BsaAI yAC'GTr
Cuts at: 810 817 827 2251 810
Size: 7 10 1424 3457
Fragments arranged by size:
3457 1424 10 7
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3279 4775 2329
Size: 248 702 1496 2452
Fragments arranged by size:
2452 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2087 2608 3286 3560 3707 4118 2087
Size: 521 678 274 147 411 2867
Fragments arranged by size:
2867 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1331 1361 1583 1613 1331
Size: 30 222 30 4616
Fragments arranged by size:
4616 222 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1471 1487 1471
Size: 16 4882
BseYI C'CCAG_C
Cuts at: 480 975 1305 2323 3081 3658 4154 480
Size: 495 330 1018 758 577 496 1224
Fragments arranged by size:
1224 1018 758 577 496 495 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1211 592
Size: 619 4279
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1912 2690 2880 3672 1912
Size: 778 190 792 3138
Fragments arranged by size:
3138 792 778 190
BsmI GAATG_Cn'
Cuts at: 1684 1684
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 1866 1912 2523 2616 2690 2880 2942 3672 1866
Size: 46 611 93 74 190 62 730 3092
Fragments arranged by size:
3092 730 611 190 93 74 62 46
BspEI T'CCGG_A
Cuts at: 4118 4118
Size: 4898
BspHI T'CATG_A
Cuts at: 488 4074 488
Size: 3586 1312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1948 2162 2464 2845 1948
Size: 214 302 381 4001
Fragments arranged by size:
4001 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 554 836 2810 262
Size: 292 282 1974 2350
Fragments arranged by size:
2350 1974 292 282
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BssSI C'ACGA_G
Cuts at: 3169 3527 3169
Size: 358 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 217 2275 217
Size: 2058 2840
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstXI CCAn_nnnn'nTGG
Cuts at: 1126 1126
Size: 4898
BstYI r'GATC_y
Cuts at: 1 1007 1923 2390 2413 2748 2994 4006 4092
Size: 1006 916 467 23 335 246 1012 86
Cuts at: 4092 4104 4450 1
Size: 12 346 449
Fragments arranged by size:
1012 1006 916 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 542 642 542
Size: 100 4798
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2848 3029 3192 2848
Size: 181 163 4554
Fragments arranged by size:
4554 181 163
BtsI GCAGTG_nn'
Cuts at: 497 2141 497
Size: 1644 3254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 734 769 734
Size: 35 4863
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3462 2604
Size: 858 4040
EaeI y'GGCC_r
Cuts at: 1619 2483 2657 3048 3075 4420 1619
Size: 864 174 391 27 1345 2097
Fragments arranged by size:
2097 1345 864 391 174 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 154 852 1171 2921 3131 154
Size: 698 319 1750 210 1921
Fragments arranged by size:
1921 1750 698 319 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3416 3562 4708 3106
Size: 310 146 1146 3296
Fragments arranged by size:
3296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1000 2723 3155 3902 4170 102
Size: 898 1723 432 747 268 830
Fragments arranged by size:
1723 898 830 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2037 2037
Size: 4898
EcoRI G'AATT_C
Cuts at: 1384 1902 1384
Size: 518 4380
EcoRV GAT'ATC
Cuts at: 1535 1535
Size: 4898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 2917 1786
Size: 1131 3767
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 2332 2580 3602 4581 2332
Size: 248 1022 979 2649
Fragments arranged by size:
2649 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1760 1792 2740 2770 2772 2802 1760
Size: 32 948 30 2 30 3856
Fragments arranged by size:
3856 948 32 30 30 2
HincII GTy'rAC
Cuts at: 1779 1937 1779
Size: 158 4740
HindIII A'AGCT_T
Cuts at: 1088 1123 1088
Size: 35 4863
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 884 1918 884
Size: 1034 3864
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 740 1104 3569 3753 740
Size: 364 2465 184 1885
Fragments arranged by size:
2465 1885 364 184
MscI TGG'CCA
Cuts at: 2659 2659
Size: 4898
MslI CAynn'nnrTG
Cuts at: 547 613 1174 1633 1753 2887 3014 547
Size: 66 561 459 120 1134 127 2431
Fragments arranged by size:
2431 1134 561 459 127 120 66
MspA1I CmG'CkG
Cuts at: 979 2009 2323 2683 3696 3941 979
Size: 1030 314 360 1013 245 1936
Fragments arranged by size:
1936 1030 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NcoI C'CATG_G
Cuts at: 542 542
Size: 4898
NdeI CA'TA_TG
Cuts at: 1731 1813 1731
Size: 82 4816
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NspI r_CATG'y
Cuts at: 1951 2451 3358 1951
Size: 500 907 3491
Fragments arranged by size:
3491 907 500
PasI CC'CwG_GG
Cuts at: 1403 1403
Size: 4898
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PflMI CCAn_nnn'nTGG
Cuts at: 248 248
Size: 4898
PfoI T'CCnGG_A
Cuts at: 714 1003 714
Size: 289 4609
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PmlI CAC'GTG
Cuts at: 810 810
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4866
PshAI GACnn'nnGTC
Cuts at: 1159 1159
Size: 4898
PsiI TTA'TAA
Cuts at: 1174 1174
Size: 4898
PstI C_TGCA'G
Cuts at: 1945 2630 1945
Size: 685 4213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 979 2323 2683 979
Size: 1344 360 3194
Fragments arranged by size:
3194 1344 360
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 2921 3131 2921
Size: 210 4688
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SfcI C'TryA_G
Cuts at: 33 566 1256 1334 1941 2626 3619 3810 33
Size: 533 690 78 607 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 607 533 191 78
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 2053 2968 3460 3722 2053
Size: 915 492 262 3229
Fragments arranged by size:
3229 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
SspI AAT'ATT
Cuts at: 1607 1607
Size: 4898
StyI C'CwwG_G
Cuts at: 203 542 1029 1157 2032 2386 203
Size: 339 487 128 875 354 2715
Fragments arranged by size:
2715 875 487 354 339 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1725 2481 2614 3073 3282 1725
Size: 756 133 459 209 3341
Fragments arranged by size:
3341 756 459 209 133
TatI w'GTAC_w
Cuts at: 1672 1816 4413 1672
Size: 144 2597 2157
Fragments arranged by size:
2597 2157 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1583 1615 4415 4447 1583
Size: 32 2800 32 2034
Fragments arranged by size:
2800 2034 32 32
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 2393 1373
Size: 1020 3878
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 115 1384 1628 115
Size: 1269 244 3385
Fragments arranged by size:
3385 1269 244
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BpmI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PpuMI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI