Restriction map for
pF1KB7231
(Circular) (Six-base) MAPSORT of: pF1KB7231.seq Check: 4907 from: 1 to: 4415
pF1KB7231 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 239 239
Size: 4415
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 550 1431 550
Size: 881 3534
AclI AA'CG_TT
Cuts at: 1267 4255 1267
Size: 2988 1427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 2240 2672 3419 3687 345
Size: 1895 432 747 268 1073
Fragments arranged by size:
1895 1073 747 432 268
AfeI AGC'GCT
Cuts at: 725 725
Size: 4415
AflIII A'CryG_T
Cuts at: 284 1767 2871 3848 284
Size: 1483 1104 977 851
Fragments arranged by size:
1483 1104 977 851
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AhdI GACnn_n'nnGTC
Cuts at: 1359 1359
Size: 4415
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 4415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 1720 3287 1720
Size: 1567 2848
ApaI G_GGCC'C
Cuts at: 347 347
Size: 4415
ApaLI G'TGCA_C
Cuts at: 253 3185 253
Size: 2932 1483
ApoI r'AATT_y
Cuts at: 1324 1419 3768 4024 1324
Size: 95 2349 256 1715
Fragments arranged by size:
2349 1715 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 339 1435 339
Size: 1096 3319
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 157 208 319 550 1431 1845 2093 2128 157
Size: 51 111 231 881 414 248 35 2444
Fragments arranged by size:
2444 881 414 248 231 111 51 35
BanII G_rGCy'C
Cuts at: 347 653 974 1429 2459 347
Size: 306 321 455 1030 2303
Fragments arranged by size:
2303 1030 455 321 306
BbeI G_GCGC'C
Cuts at: 161 1849 2097 161
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
BbsI GAAGACnn'nnnn_
Cuts at: 351 351
Size: 4415
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglI GCCn_nnn'nGGC
Cuts at: 351 351
Size: 4415
BglII A'GATC_T
Cuts at: 569 1930 3967 569
Size: 1361 2037 1017
Fragments arranged by size:
2037 1361 1017
BlpI GC'TnA_GC
Cuts at: 968 1527 968
Size: 559 3856
Bme1580I G_kGCm'C
Cuts at: 257 322 347 2040 2133 3189 257
Size: 65 25 1693 93 1056 1483
Fragments arranged by size:
1693 1483 1056 93 65 25
BmrI ACTGGGnnnn_n'
Cuts at: 417 545 1220 1627 1708 2043 4187 417
Size: 128 675 407 81 335 2144 645
Fragments arranged by size:
2144 675 645 407 335 128 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 2782 195
Size: 2587 1828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 331 259
Size: 72 4343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 1591 2470 2962 3260 1222
Size: 369 879 492 298 2377
Fragments arranged by size:
2377 879 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 1251 4119 694
Size: 557 2868 990
Fragments arranged by size:
2868 990 557
BsaAI yAC'GTr
Cuts at: 285 821 1768 285
Size: 536 947 2932
Fragments arranged by size:
2932 947 536
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 158 1846 2094 2796 4292 158
Size: 1688 248 702 1496 281
Fragments arranged by size:
1688 1496 702 281 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1391 1394 1421 1424 1391
Size: 3 27 3 4382
Fragments arranged by size:
4382 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 1415 1418 592
Size: 823 3 3589
Fragments arranged by size:
3589 823 3
BseYI C'CCAG_C
Cuts at: 977 1840 2598 3175 3671 977
Size: 863 758 577 496 1721
Fragments arranged by size:
1721 863 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1032 1394 1957 2003 3211 104
Size: 928 362 563 46 1208 1308
Fragments arranged by size:
1308 1208 928 563 362 46
BsiHKAI G_wGCw'C
Cuts at: 125 257 302 974 1429 2207 2397 3189 125
Size: 132 45 672 455 778 190 792 1351
Fragments arranged by size:
1351 792 778 672 455 190 132 45
BsmI GAATG_Cn'
Cuts at: 852 852
Size: 4415
Bsp1286I G_dGCh'C
Cuts at: 125 257 302 322 347 653 974 1429 2040
Size: 132 45 20 25 306 321 455 611
Cuts at: 2040 2133 2207 2397 2459 3189 125
Size: 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 455 321 306 190 132
93 74 62 45 25 20
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 3591 3591
Size: 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 269 345 863 1465 1679 1981 2362 239
Size: 30 76 518 602 214 302 381 2292
Fragments arranged by size:
2292 602 518 381 302 214 76 30
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 2413 2594 2803 2413
Size: 181 209 4025
Fragments arranged by size:
4025 209 181
BssSI C'ACGA_G
Cuts at: 262 301 2686 3044 262
Size: 39 2385 358 1633
Fragments arranged by size:
2385 1633 358 39
BstAPI GCAn_nnn'nTGC
Cuts at: 1792 1792
Size: 4415
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstEII G'GTnAC_C
Cuts at: 775 813 775
Size: 38 4377
BstXI CCAn_nnnn'nTGG
Cuts at: 876 876
Size: 4415
BstYI r'GATC_y
Cuts at: 1 306 569 1440 1907 1930 2265 2511 3523
Size: 305 263 871 467 23 335 246 1012
Cuts at: 3523 3609 3621 3967 1
Size: 86 12 346 449
Fragments arranged by size:
1012 871 467 449 346 335 305 263
246 86 23 12
BtgI C'CryG_G
Cuts at: 216 323 641 1388 216
Size: 107 318 747 3243
Fragments arranged by size:
3243 747 318 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 145 1658 145
Size: 1513 2902
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DraIII CAC_nnn'GTG
Cuts at: 792 821 792
Size: 29 4386
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 866 1029 1391 2000 2174 2565 2592 3937 866
Size: 163 362 609 174 391 27 1345 1344
Fragments arranged by size:
1345 1344 609 391 362 174 163 27
EagI C'GGCC_G
Cuts at: 1029 1391 2000 1029
Size: 362 609 3444
Fragments arranged by size:
3444 609 362
EarI CTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 2933 3079 4225 2623
Size: 310 146 1146 2813
Fragments arranged by size:
2813 1146 310 146
EcoICRI GAG'CTC
Cuts at: 972 1427 972
Size: 455 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 331 345 2240 2672 3419 3687 259
Size: 72 14 1895 432 747 268 987
Fragments arranged by size:
1895 987 747 432 268 72 14
EcoO109I rG'GnC_Cy
Cuts at: 343 344 746 1554 343
Size: 1 402 808 3204
Fragments arranged by size:
3204 808 402 1
EcoRI G'AATT_C
Cuts at: 1324 1419 1324
Size: 95 4320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 161 727 1849 2097 3119 4098 161
Size: 566 1122 248 1022 979 478
Fragments arranged by size:
1122 1022 979 566 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1345 1377 2257 2287 2289 2319 1345
Size: 32 880 30 2 30 3441
Fragments arranged by size:
3441 880 32 30 30 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 157 1845 2093 157
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
KpnI G_GTAC'C
Cuts at: 554 1435 554
Size: 881 3534
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3086 3270 3086
Size: 184 4231
MscI TGG'CCA
Cuts at: 868 2176 868
Size: 1308 3107
MslI CAynn'nnrTG
Cuts at: 250 643 874 1072 2404 2531 250
Size: 393 231 198 1332 127 2134
Fragments arranged by size:
2134 1332 393 231 198 127
MspA1I CmG'CkG
Cuts at: 205 237 272 510 843 1526 1840 2200 3213
Size: 32 35 238 333 683 314 360 1013
Cuts at: 3213 3458 205
Size: 245 1162
Fragments arranged by size:
1162 1013 683 360 333 314 245 238
35 32
NaeI GCC'GGC
Cuts at: 2596 2596
Size: 4415
NarI GG'CG_CC
Cuts at: 158 1846 2094 158
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
NcoI C'CATG_G
Cuts at: 323 323
Size: 4415
NdeI CA'TA_TG
Cuts at: 736 736
Size: 4415
NgoMIV G'CCGG_C
Cuts at: 2594 2594
Size: 4415
NspI r_CATG'y
Cuts at: 193 1071 1468 1968 2875 193
Size: 878 397 500 907 1733
Fragments arranged by size:
1733 907 878 500 397
PasI CC'CwG_GG
Cuts at: 522 1181 522
Size: 659 3756
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 167 522 167
Size: 355 4060
PfoI T'CCnGG_A
Cuts at: 742 1229 742
Size: 487 3928
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PmlI CAC'GTG
Cuts at: 821 821
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 746 746
Size: 4415
PshAI GACnn'nnGTC
Cuts at: 1022 1022
Size: 4415
PsiI TTA'TAA
Cuts at: 598 598
Size: 4415
PspOMI G'GGCC_C
Cuts at: 343 343
Size: 4415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1108 1140 1108
Size: 32 4383
PstI C_TGCA'G
Cuts at: 236 500 1462 2147 236
Size: 264 962 685 2504
Fragments arranged by size:
2504 962 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 237 510 843 1840 2200 237
Size: 273 333 997 360 2452
Fragments arranged by size:
2452 997 360 333 273
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 974 1429 974
Size: 455 3960
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 500 1462 500
Size: 962 3453
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SexAI A'CCwGG_T
Cuts at: 788 788
Size: 4415
SfcI C'TryA_G
Cuts at: 33 232 496 1458 2143 3136 3327 33
Size: 199 264 962 685 993 191 1121
Fragments arranged by size:
1121 993 962 685 264 199 191
SfoI GGC'GCC
Cuts at: 159 1847 2095 159
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 1237 1570 2485 2977 3239 1237
Size: 333 915 492 262 2413
Fragments arranged by size:
2413 915 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1071 1468 1968 1071
Size: 397 500 3518
Fragments arranged by size:
3518 500 397
StuI AGG'CCT
Cuts at: 1282 1282
Size: 4415
StyI C'CwwG_G
Cuts at: 323 413 626 1549 1903 323
Size: 90 213 923 354 2835
Fragments arranged by size:
2835 923 354 213 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 514 780 991 1998 2131 2590 2799 514
Size: 266 211 1007 133 459 209 2130
Fragments arranged by size:
2130 1007 459 266 211 209 133
TatI w'GTAC_w
Cuts at: 3930 3930
Size: 4415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 447 463 537 2212 447
Size: 16 74 1675 2650
Fragments arranged by size:
2650 1675 74 16
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 523 1080 1910 523
Size: 557 830 3028
Fragments arranged by size:
3028 830 557
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI