Restriction map for
pF1KB7233
(Circular) (Six-base) MAPSORT of: pF1KB7233.seq Check: 7069 from: 1 to: 4460
pF1KB7233 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 283 1498 283
Size: 1215 3245
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1025 2285 2717 3464 3732 820
Size: 205 1260 432 747 268 1548
Fragments arranged by size:
1548 1260 747 432 268 205
AfeI AGC'GCT
Cuts at: 697 697
Size: 4460
AflIII A'CryG_T
Cuts at: 1812 2916 3893 1812
Size: 1104 977 2379
Fragments arranged by size:
2379 1104 977
AgeI A'CCGG_T
Cuts at: 1414 2848 1414
Size: 1434 3026
AhdI GACnn_n'nnGTC
Cuts at: 561 561
Size: 4460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 509 1033 509
Size: 524 3936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 973 974 1005 1006 973
Size: 1 31 1 4427
Fragments arranged by size:
4427 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 308 1275 1765 3332 308
Size: 967 490 1567 1436
Fragments arranged by size:
1567 1436 967 490
ApaLI G'TGCA_C
Cuts at: 3230 3230
Size: 4460
ApoI r'AATT_y
Cuts at: 1464 3813 4069 1464
Size: 2349 256 1855
Fragments arranged by size:
2349 1855 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 517 1480 517
Size: 963 3497
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 200 1476 1890 2138 2173 200
Size: 1276 414 248 35 2487
Fragments arranged by size:
2487 1276 414 248 35
BanII G_rGCy'C
Cuts at: 590 902 942 1474 2504 590
Size: 312 40 532 1030 2546
Fragments arranged by size:
2546 1030 532 312 40
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3119 4460 2358
Size: 761 1341 2358
Fragments arranged by size:
2358 1341 761
BclI T'GATC_A
Cuts at: 1980 1980
Size: 4460
BglI GCCn_nnn'nGGC
Cuts at: 253 253
Size: 4460
BglII A'GATC_T
Cuts at: 1975 4012 1975
Size: 2037 2423
BlpI GC'TnA_GC
Cuts at: 1572 1572
Size: 4460
Bme1580I G_kGCm'C
Cuts at: 553 2085 2178 3234 553
Size: 1532 93 1056 1779
Fragments arranged by size:
1779 1532 1056 93
BmgBI CAC'GTC
Cuts at: 1284 1284
Size: 4460
BmrI ACTGGGnnnn_n'
Cuts at: 993 1672 1753 2088 4232 993
Size: 679 81 335 2144 1221
Fragments arranged by size:
2144 1221 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 831 2827 831
Size: 1996 2464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 484 502 523 574 706 799 1090 1117
Size: 302 18 21 51 132 93 291 27
Cuts at: 1117 1267 1351 182
Size: 150 84 3291
Fragments arranged by size:
3291 302 291 150 132 93 84 51
27 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1636 2515 3007 3305 805
Size: 831 879 492 298 1960
Fragments arranged by size:
1960 879 831 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 499 955 1427 4164 418
Size: 81 456 472 2737 714
Fragments arranged by size:
2737 714 472 456 81
BsaAI yAC'GTr
Cuts at: 1813 1813
Size: 4460
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 878 1319 1414 1649 2170 2848 3122 3269 3680
Size: 441 95 235 521 678 274 147 411
Cuts at: 3680 878
Size: 1658
Fragments arranged by size:
1658 678 521 441 411 274 235 147
95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 141 142 171 1033 1063 112
Size: 29 1 29 862 30 3509
Fragments arranged by size:
3509 862 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 505 537 598 820 870 1054 120
Size: 385 32 61 222 50 184 3526
Fragments arranged by size:
3526 385 222 184 61 50 32
BseYI C'CCAG_C
Cuts at: 301 785 864 1115 1885 2643 3220 3716 301
Size: 484 79 251 770 758 577 496 1045
Fragments arranged by size:
1045 770 758 577 496 484 251 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 627
Size: 4460
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 590 1474 2252 2442 3234 590
Size: 884 778 190 792 1816
Fragments arranged by size:
1816 884 792 778 190
BsmI GAATG_Cn'
Cuts at: 498 627 1095 498
Size: 129 468 3863
Fragments arranged by size:
3863 468 129
Bsp1286I G_dGCh'C
Cuts at: 553 590 902 942 1474 2085 2178 2252 2442
Size: 37 312 40 532 611 93 74 190
Cuts at: 2442 2504 3234 553
Size: 62 730 1779
Fragments arranged by size:
1779 730 611 532 312 190 93 74
62 40 37
BspEI T'CCGG_A
Cuts at: 878 3680 878
Size: 2802 1658
BspHI T'CATG_A
Cuts at: 3636 3636
Size: 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1510 1724 2026 2407 1510
Size: 214 302 381 3563
Fragments arranged by size:
3563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1251 2752 2806 4290 4455 50
Size: 1201 1501 54 1484 165 55
Fragments arranged by size:
1501 1484 1201 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2372 2372
Size: 4460
BsrFI r'CCGG_y
Cuts at: 1414 2458 2639 2848 1414
Size: 1044 181 209 3026
Fragments arranged by size:
3026 1044 209 181
BsrGI T'GTAC_A
Cuts at: 636 636
Size: 4460
BssSI C'ACGA_G
Cuts at: 535 832 2731 3089 535
Size: 297 1899 358 1906
Fragments arranged by size:
1906 1899 358 297
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 1837
Size: 4460
BstBI TT'CG_AA
Cuts at: 2821 2821
Size: 4460
BstXI CCAn_nnnn'nTGG
Cuts at: 462 462
Size: 4460
BstYI r'GATC_y
Cuts at: 1 599 1064 1232 1485 1952 1975 2310 2556
Size: 598 465 168 253 467 23 335 246
Cuts at: 2556 3568 3654 3666 4012 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 598 467 465 449 346 335 253
246 168 86 23 12
BtgI C'CryG_G
Cuts at: 152 953 1434 152
Size: 801 481 3178
Fragments arranged by size:
3178 801 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 329 1054 1703 329
Size: 725 649 3086
Fragments arranged by size:
3086 725 649
ClaI AT'CG_AT
Cuts at: 191 191
Size: 4460
DraI TTT'AAA
Cuts at: 1459 1459
Size: 4460
DraIII CAC_nnn'GTG
Cuts at: 548 548
Size: 4460
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3024 2166
Size: 858 3602
EaeI y'GGCC_r
Cuts at: 245 411 452 764 1431 2045 2219 2610 2637
Size: 166 41 312 667 614 174 391 27
Cuts at: 2637 3982 245
Size: 1345 723
Fragments arranged by size:
1345 723 667 614 391 312 174 166
41 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 1211 2483 2693 1211
Size: 1272 210 2978
Fragments arranged by size:
2978 1272 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 736 2668 2978 3124 4270 736
Size: 1932 310 146 1146 926
Fragments arranged by size:
1932 1146 926 310 146
EcoICRI GAG'CTC
Cuts at: 588 1472 588
Size: 884 3576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 484 502 523 574 706 799 820 1025
Size: 302 18 21 51 132 93 21 205
Cuts at: 1025 1090 1117 1267 1351 2285 2717 3464 3732
Size: 65 27 150 84 934 432 747 268
Cuts at: 3732 182
Size: 910
Fragments arranged by size:
934 910 747 432 302 268 205 150
132 93 84 65 51 27 21 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 466 766 802 466
Size: 300 36 4124
Fragments arranged by size:
4124 300 36
EcoO109I rG'GnC_Cy
Cuts at: 350 386 1599 350
Size: 36 1213 3211
Fragments arranged by size:
3211 1213 36
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4460
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 699 1110 1894 2142 3164 4143 699
Size: 411 784 248 1022 979 1016
Fragments arranged by size:
1022 1016 979 784 411 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 924 956 1210 1242 2302 2332 2334
Size: 32 345 32 254 32 1060 30 2
Cuts at: 2334 2364 547
Size: 30 2643
Fragments arranged by size:
2643 1060 345 254 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 284 1499 284
Size: 1215 3245
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1040 1277 3131 3315 1040
Size: 237 1854 184 2185
Fragments arranged by size:
2185 1854 237 184
MscI TGG'CCA
Cuts at: 247 413 454 766 1433 2221 247
Size: 166 41 312 667 788 2486
Fragments arranged by size:
2486 788 667 312 166 41
MslI CAynn'nnrTG
Cuts at: 974 2449 2576 974
Size: 1475 127 2858
Fragments arranged by size:
2858 1475 127
MspA1I CmG'CkG
Cuts at: 480 711 864 1571 1885 2245 3258 3503 480
Size: 231 153 707 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 707 360 314 245 231 153
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NcoI C'CATG_G
Cuts at: 152 953 1434 152
Size: 801 481 3178
Fragments arranged by size:
3178 801 481
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 1513 2013 2920 1513
Size: 500 907 3053
Fragments arranged by size:
3053 907 500
PasI CC'CwG_GG
Cuts at: 291 291
Size: 4460
PciI A'CATG_T
Cuts at: 2916 2916
Size: 4460
PflMI CCAn_nnn'nTGG
Cuts at: 210 714 978 1402 210
Size: 504 264 424 3268
Fragments arranged by size:
3268 504 424 264
PfoI T'CCnGG_A
Cuts at: 159 746 1067 159
Size: 587 321 3552
Fragments arranged by size:
3552 587 321
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 350 350
Size: 4460
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1249 1281 1249
Size: 32 4428
PstI C_TGCA'G
Cuts at: 1022 1049 1142 1507 2192 1022
Size: 27 93 365 685 3290
Fragments arranged by size:
3290 685 365 93 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 480 711 864 1885 2245 480
Size: 231 153 1021 360 2695
Fragments arranged by size:
2695 1021 360 231 153
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 590 1474 590
Size: 884 3576
SalI G'TCGA_C
Cuts at: 282 1497 282
Size: 1215 3245
SapI GCTCTTCn'nnn_
Cuts at: 1211 2483 2693 1211
Size: 1272 210 2978
Fragments arranged by size:
2978 1272 210
SbfI CC_TGCA'GG
Cuts at: 1022 1507 1022
Size: 485 3975
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SexAI A'CCwGG_T
Cuts at: 1268 1268
Size: 4460
SfcI C'TryA_G
Cuts at: 33 120 1018 1045 1138 1503 2188 3181 3372
Size: 87 898 27 93 365 685 993 191
Cuts at: 3372 33
Size: 1121
Fragments arranged by size:
1121 993 898 685 365 191 93 87
27
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SgrAI Cr'CCGG_yG
Cuts at: 1414 1414
Size: 4460
SmaI CCC'GGG
Cuts at: 1482 1482
Size: 4460
SmlI C'TyrA_G
Cuts at: 517 820 1615 2530 3022 3284 517
Size: 303 795 915 492 262 1693
Fragments arranged by size:
1693 915 795 492 303 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1513 2013 1513
Size: 500 3960
StyI C'CwwG_G
Cuts at: 152 767 953 1434 1594 1948 152
Size: 615 186 481 160 354 2664
Fragments arranged by size:
2664 615 481 354 186 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 2043 2176 2635 2844 188
Size: 1855 133 459 209 1804
Fragments arranged by size:
1855 1804 459 209 133
TatI w'GTAC_w
Cuts at: 636 1384 3975 636
Size: 748 2591 1121
Fragments arranged by size:
2591 1121 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 141 173 321 353 1033 1065 3977 4009 141
Size: 32 148 32 680 32 2912 32 592
Fragments arranged by size:
2912 680 592 148 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 774 1955 240
Size: 534 1181 2745
Fragments arranged by size:
2745 1181 534
XhoI C'TCGA_G
Cuts at: 517 517
Size: 4460
XmaI C'CCGG_G
Cuts at: 1480 1480
Size: 4460
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SfiI SnaBI SrfI SspI StuI SwaI XmnI ZraI