Restriction map for
pF1KB7234
(Circular) (Six-base) MAPSORT of: pF1KB7234.seq Check: 2167 from: 1 to: 4277
pF1KB7234 4277 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 244 244
Size: 4277
AatII G_ACGT'C
Cuts at: 1066 1066
Size: 4277
AccI GT'mk_AC
Cuts at: 1031 1315 1031
Size: 284 3993
Acc65I G'GTAC_C
Cuts at: 581 1293 581
Size: 712 3565
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2534 3281 3549 2102
Size: 432 747 268 2830
Fragments arranged by size:
2830 747 432 268
AflIII A'CryG_T
Cuts at: 838 1629 2733 3710 838
Size: 791 1104 977 1405
Fragments arranged by size:
1405 1104 977 791
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AleI CACnn'nnGTG
Cuts at: 829 829
Size: 4277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1019 1019
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 211 723 1582 3149 211
Size: 512 859 1567 1339
Fragments arranged by size:
1567 1339 859 512
ApaI G_GGCC'C
Cuts at: 120 120
Size: 4277
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 1261 1281 3630 3886 1261
Size: 20 2349 256 1652
Fragments arranged by size:
2349 1652 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 1297 1297
Size: 4277
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 675 708 675
Size: 33 4244
BamHI G'GATC_C
Cuts at: 371 1302 371
Size: 931 3346
BanI G'GyrC_C
Cuts at: 310 581 967 1293 1707 1955 1990 310
Size: 271 386 326 414 248 35 2597
Fragments arranged by size:
2597 414 386 326 271 248 35
BanII G_rGCy'C
Cuts at: 120 536 1291 2321 120
Size: 416 755 1030 2076
Fragments arranged by size:
2076 1030 755 416
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BbsI GAAGACnn'nnnn_
Cuts at: 440 440
Size: 4277
BbvCI CC'TCA_GC
Cuts at: 212 212
Size: 4277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1024 1058 1024
Size: 34 4243
BciVI GTATCCnnnnn_n'
Cuts at: 963 2175 2936 4277 963
Size: 1212 761 1341 963
Fragments arranged by size:
1341 1212 963 761
BclI T'GATC_A
Cuts at: 1115 1797 1115
Size: 682 3595
BglII A'GATC_T
Cuts at: 1792 3829 1792
Size: 2037 2240
BlpI GC'TnA_GC
Cuts at: 535 1389 535
Size: 854 3423
Bme1580I G_kGCm'C
Cuts at: 120 1902 1995 3051 120
Size: 1782 93 1056 1346
Fragments arranged by size:
1782 1346 1056 93
BmgBI CAC'GTC
Cuts at: 839 839
Size: 4277
BmrI ACTGGGnnnn_n'
Cuts at: 1489 1570 1905 4049 1489
Size: 81 335 2144 1717
Fragments arranged by size:
2144 1717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 949 2644 369
Size: 580 1695 2002
Fragments arranged by size:
2002 1695 580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 660 415
Size: 245 4032
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 4277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1453 2332 2824 3122 364
Size: 1089 879 492 298 1519
Fragments arranged by size:
1519 1089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3981 3981
Size: 4277
BsaAI yAC'GTr
Cuts at: 756 1020 1630 756
Size: 264 610 3403
Fragments arranged by size:
3403 610 264
BsaBI GATnn'nnATC
Cuts at: 6 1791 3828 6
Size: 1785 2037 455
Fragments arranged by size:
2037 1785 455
BsaHI Gr'CG_yC
Cuts at: 985 1063 1708 1956 2658 4154 985
Size: 78 645 248 702 1496 1108
Fragments arranged by size:
1496 1108 702 645 248 78
BsaWI w'CCGG_w
Cuts at: 730 1466 1987 2665 2939 3086 3497 730
Size: 736 521 678 274 147 411 1510
Fragments arranged by size:
1510 736 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 361
Size: 4277
BseYI C'CCAG_C
Cuts at: 282 1702 2460 3037 3533 282
Size: 1420 758 577 496 1026
Fragments arranged by size:
1420 1026 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 787 229
Size: 558 3719
BsiEI CG_ry'CG
Cuts at: 104 158 1819 1865 3073 104
Size: 54 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 54 46
BsiHKAI G_wGCw'C
Cuts at: 536 1150 1291 2069 2259 3051 536
Size: 614 141 778 190 792 1762
Fragments arranged by size:
1762 792 778 614 190 141
BsmBI CGTCTCn'nnnn_
Cuts at: 922 922
Size: 4277
Bsp1286I G_dGCh'C
Cuts at: 120 536 1150 1291 1902 1995 2069 2259 2321
Size: 416 614 141 611 93 74 190 62
Cuts at: 2321 3051 120
Size: 730 1346
Fragments arranged by size:
1346 730 614 611 416 190 141 93
74 62
BspEI T'CCGG_A
Cuts at: 730 3497 730
Size: 2767 1510
BspHI T'CATG_A
Cuts at: 3453 3453
Size: 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 1327 1541 1843 2224 244
Size: 1083 214 302 381 2297
Fragments arranged by size:
2297 1083 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2569 2623 4107 4272 50
Size: 2519 54 1484 165 55
Fragments arranged by size:
2519 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2189 2189
Size: 4277
BsrFI r'CCGG_y
Cuts at: 2275 2456 2665 2275
Size: 181 209 3887
Fragments arranged by size:
3887 209 181
BssSI C'ACGA_G
Cuts at: 2548 2906 2548
Size: 358 3919
BstAPI GCAn_nnn'nTGC
Cuts at: 756 1654 756
Size: 898 3379
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstXI CCAn_nnnn'nTGG
Cuts at: 256 628 1194 256
Size: 372 566 3339
Fragments arranged by size:
3339 566 372
BstYI r'GATC_y
Cuts at: 1 371 872 1302 1769 1792 2127 2373 3385
Size: 370 501 430 467 23 335 246 1012
Cuts at: 3385 3471 3483 3829 1
Size: 86 12 346 449
Fragments arranged by size:
1012 501 467 449 430 370 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 158 550 779 885 920 956 107
Size: 51 392 229 106 35 36 3428
Fragments arranged by size:
3428 392 229 106 51 36 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2227 2408 2571 2227
Size: 181 163 3933
Fragments arranged by size:
3933 181 163
BtsI GCAGTG_nn'
Cuts at: 1520 1520
Size: 4277
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1983 2841 1983
Size: 858 3419
EaeI y'GGCC_r
Cuts at: 133 155 999 1862 2036 2427 2454 3799 133
Size: 22 844 863 174 391 27 1345 611
Fragments arranged by size:
1345 863 844 611 391 174 27 22
EagI C'GGCC_G
Cuts at: 155 1862 155
Size: 1707 2570
EarI CTCTTCn'nnn_
Cuts at: 304 2300 2510 304
Size: 1996 210 2071
Fragments arranged by size:
2071 1996 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 928 2485 2795 2941 4087 928
Size: 1557 310 146 1146 1118
Fragments arranged by size:
1557 1146 1118 310 146
EcoICRI GAG'CTC
Cuts at: 534 1289 534
Size: 755 3522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 660 2102 2534 3281 3549 415
Size: 245 1442 432 747 268 1143
Fragments arranged by size:
1442 1143 747 432 268 245
EcoO109I rG'GnC_Cy
Cuts at: 1416 1416
Size: 4277
EcoRI G'AATT_C
Cuts at: 1261 1281 1261
Size: 20 4257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 4277
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 126 724 1015 1102 1711 1959 2981 3960 126
Size: 598 291 87 609 248 1022 979 443
Fragments arranged by size:
1022 979 609 598 443 291 248 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2119 2149 2151 2181 2119
Size: 30 2 30 4215
Fragments arranged by size:
4215 30 30 2
HincII GTy'rAC
Cuts at: 836 1316 836
Size: 480 3797
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 585 1297 585
Size: 712 3565
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3132 2948
Size: 184 4093
MscI TGG'CCA
Cuts at: 2038 2038
Size: 4277
MslI CAynn'nnrTG
Cuts at: 251 829 1001 1123 2266 2393 251
Size: 578 172 122 1143 127 2135
Fragments arranged by size:
2135 1143 578 172 127 122
MspA1I CmG'CkG
Cuts at: 138 160 539 552 878 887 958 1388 1702
Size: 22 379 13 326 9 71 430 314
Cuts at: 1702 2062 3075 3320 138
Size: 360 1013 245 1095
Fragments arranged by size:
1095 1013 430 379 360 326 314 245
71 22 13 9
NaeI GCC'GGC
Cuts at: 2458 2458
Size: 4277
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NcoI C'CATG_G
Cuts at: 107 107
Size: 4277
NdeI CA'TA_TG
Cuts at: 1120 1120
Size: 4277
NgoMIV G'CCGG_C
Cuts at: 2456 2456
Size: 4277
NotI GC'GGCC_GC
Cuts at: 155 155
Size: 4277
NspI r_CATG'y
Cuts at: 1330 1830 2737 1330
Size: 500 907 2870
Fragments arranged by size:
2870 907 500
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PflMI CCAn_nnn'nTGG
Cuts at: 807 807
Size: 4277
PfoI T'CCnGG_A
Cuts at: 500 500
Size: 4277
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PspOMI G'GGCC_C
Cuts at: 116 116
Size: 4277
PstI C_TGCA'G
Cuts at: 1324 2009 1324
Size: 685 3592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 539 1702 2062 539
Size: 1163 360 2754
Fragments arranged by size:
2754 1163 360
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 536 1291 536
Size: 755 3522
SacII CC_GC'GG
Cuts at: 161 553 888 959 161
Size: 392 335 71 3479
Fragments arranged by size:
3479 392 335 71
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 772 3794 772
Size: 3022 1255
SfcI C'TryA_G
Cuts at: 33 1320 2005 2998 3189 33
Size: 1287 685 993 191 1121
Fragments arranged by size:
1287 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 343 1432 2347 2839 3101 343
Size: 1089 915 492 262 1519
Fragments arranged by size:
1519 1089 915 492 262
SnaBI TAC'GTA
Cuts at: 1020 1020
Size: 4277
SpeI A'CTAG_T
Cuts at: 67 468 67
Size: 401 3876
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StyI C'CwwG_G
Cuts at: 107 1411 1765 107
Size: 1304 354 2619
Fragments arranged by size:
2619 1304 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 454 1860 1993 2452 2661 454
Size: 1406 133 459 209 2070
Fragments arranged by size:
2070 1406 459 209 133
TatI w'GTAC_w
Cuts at: 186 770 3792 186
Size: 584 3022 671
Fragments arranged by size:
3022 671 584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4245
Tth111I GACn'n_nGTC
Cuts at: 2074 2074
Size: 4277
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1186 1772 1186
Size: 586 3691
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
ZraI GAC'GTC
Cuts at: 1064 1064
Size: 4277
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NotI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BfrBI BglI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI