Restriction map for
pF1KB7235
(Circular) (Six-base) MAPSORT of: pF1KB7235.seq Check: 1012 from: 1 to: 5018
pF1KB7235 5018 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 2056 2056
Size: 5018
Acc65I G'GTAC_C
Cuts at: 1088 2034 1088
Size: 946 4072
AclI AA'CG_TT
Cuts at: 4858 4858
Size: 5018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 541 785 1015 2843 3275 4022 4290 241
Size: 300 244 230 1828 432 747 268 969
Fragments arranged by size:
1828 969 747 432 300 268 244 230
AflIII A'CryG_T
Cuts at: 380 2370 3474 4451 380
Size: 1990 1104 977 947
Fragments arranged by size:
1990 1104 977 947
AgeI A'CCGG_T
Cuts at: 3406 3406
Size: 5018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1089 1676 1089
Size: 587 4431
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1790 1822 1790
Size: 32 4986
AlwNI CAG_nnn'CTG
Cuts at: 303 718 2323 3890 303
Size: 415 1605 1567 1431
Fragments arranged by size:
1605 1567 1431 415
ApaI G_GGCC'C
Cuts at: 123 1440 123
Size: 1317 3701
ApaLI G'TGCA_C
Cuts at: 3788 3788
Size: 5018
ApoI r'AATT_y
Cuts at: 2022 4371 4627 2022
Size: 2349 256 2413
Fragments arranged by size:
2413 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5018
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
AvaI C'yCGr_G
Cuts at: 1221 1432 1439 2038 1221
Size: 211 7 599 4201
Fragments arranged by size:
4201 599 211 7
AvrII C'CTAG_G
Cuts at: 1722 1722
Size: 5018
BamHI G'GATC_C
Cuts at: 916 1259 2043 916
Size: 343 784 3891
Fragments arranged by size:
3891 784 343
BanI G'GyrC_C
Cuts at: 1088 2034 2448 2696 2731 1088
Size: 946 414 248 35 3375
Fragments arranged by size:
3375 946 414 248 35
BanII G_rGCy'C
Cuts at: 123 218 1249 1440 1534 1759 2032 3062 123
Size: 95 1031 191 94 225 273 1030 2079
Fragments arranged by size:
2079 1031 1030 273 225 191 95 94
BbeI G_GCGC'C
Cuts at: 2452 2700 2452
Size: 248 4770
BbvCI CC'TCA_GC
Cuts at: 1618 1645 1618
Size: 27 4991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 365 399 90
Size: 34 241 34 4709
Fragments arranged by size:
4709 241 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2916 3677 5018 2916
Size: 761 1341 2916
Fragments arranged by size:
2916 1341 761
BclI T'GATC_A
Cuts at: 2538 2538
Size: 5018
BglII A'GATC_T
Cuts at: 1307 2533 4570 1307
Size: 1226 2037 1755
Fragments arranged by size:
2037 1755 1226
BlpI GC'TnA_GC
Cuts at: 743 2130 743
Size: 1387 3631
Bme1580I G_kGCm'C
Cuts at: 123 1068 1440 1930 2643 2736 3792 123
Size: 945 372 490 713 93 1056 1349
Fragments arranged by size:
1349 1056 945 713 490 372 93
BmrI ACTGGGnnnn_n'
Cuts at: 278 509 2230 2311 2646 4790 278
Size: 231 1721 81 335 2144 506
Fragments arranged by size:
2144 1721 506 335 231 81
BmtI G_CTAG'C
Cuts at: 1037 1037
Size: 5018
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 829 971 1296 1320 1441 1474 1548 566
Size: 263 142 325 24 121 33 74 4036
Fragments arranged by size:
4036 325 263 142 121 74 33 24
Bpu10I CC'TnA_GC
Cuts at: 1618 1645 1753 1618
Size: 27 108 4883
Fragments arranged by size:
4883 108 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 3073 3565 3863 2194
Size: 879 492 298 3349
Fragments arranged by size:
3349 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1360 1552 1564 4722 1360
Size: 192 12 3158 1656
Fragments arranged by size:
3158 1656 192 12
BsaAI yAC'GTr
Cuts at: 381 2371 381
Size: 1990 3028
BsaBI GATnn'nnATC
Cuts at: 6 2532 4569 6
Size: 2526 2037 455
Fragments arranged by size:
2526 2037 455
BsaHI Gr'CG_yC
Cuts at: 2449 2697 3399 4895 2449
Size: 248 702 1496 2572
Fragments arranged by size:
2572 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2207 2728 3406 3680 3827 4238 2207
Size: 521 678 274 147 411 2987
Fragments arranged by size:
2987 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 944 974 1231 1261 1790 1820 944
Size: 30 257 30 529 30 4142
Fragments arranged by size:
4142 529 257 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 586 1457 136
Size: 450 871 3697
Fragments arranged by size:
3697 871 450
BseYI C'CCAG_C
Cuts at: 566 1611 1801 2443 3201 3778 4274 566
Size: 1045 190 642 758 577 496 1310
Fragments arranged by size:
1310 1045 758 642 577 496 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1646 1696 412
Size: 1234 50 3734
Fragments arranged by size:
3734 1234 50
BsiEI CG_ry'CG
Cuts at: 104 2560 2606 3814 104
Size: 2456 46 1208 1308
Fragments arranged by size:
2456 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 744 1534 2032 2810 3000 3792 744
Size: 790 498 778 190 792 1970
Fragments arranged by size:
1970 792 790 778 498 190
BsmBI CGTCTCn'nnnn_
Cuts at: 418 418
Size: 5018
Bsp1286I G_dGCh'C
Cuts at: 123 218 744 1068 1249 1440 1534 1759 1930
Size: 95 526 324 181 191 94 225 171
Cuts at: 1930 2032 2643 2736 2810 3000 3062 3792 123
Size: 102 611 93 74 190 62 730 1349
Fragments arranged by size:
1349 730 611 526 324 225 191 190
181 171 102 95 94 93 74 62
BspEI T'CCGG_A
Cuts at: 4238 4238
Size: 5018
BspHI T'CATG_A
Cuts at: 4194 4194
Size: 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 691 953 1718 2068 2282 2584 2965 691
Size: 262 765 350 214 302 381 2744
Fragments arranged by size:
2744 765 381 350 302 262 214
BsrBI CCG'CTC
Cuts at: 50 1597 3310 3364 4848 5013 50
Size: 1547 1713 54 1484 165 55
Fragments arranged by size:
1713 1547 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 982 1967 2930 982
Size: 985 963 3070
Fragments arranged by size:
3070 985 963
BsrFI r'CCGG_y
Cuts at: 504 3016 3197 3406 504
Size: 2512 181 209 2116
Fragments arranged by size:
2512 2116 209 181
BssHII G'CGCG_C
Cuts at: 1491 1491
Size: 5018
BssSI C'ACGA_G
Cuts at: 1533 3289 3647 1533
Size: 1756 358 2904
Fragments arranged by size:
2904 1756 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1608 2395 1608
Size: 787 4231
BstBI TT'CG_AA
Cuts at: 3379 3379
Size: 5018
BstEII G'GTnAC_C
Cuts at: 132 1209 1590 132
Size: 1077 381 3560
Fragments arranged by size:
3560 1077 381
BstXI CCAn_nnnn'nTGG
Cuts at: 1352 1352
Size: 5018
BstYI r'GATC_y
Cuts at: 1 630 893 916 1259 1307 1580 2043 2510
Size: 629 263 23 343 48 273 463 467
Cuts at: 2510 2533 2868 3114 4126 4212 4224 4570 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 629 467 463 449 346 343 335
273 263 246 86 48 23 23 12
Bsu36I CC'TnA_GG
Cuts at: 601 1735 601
Size: 1134 3884
BtgI C'CryG_G
Cuts at: 316 530 653 1934 316
Size: 214 123 1281 3400
Fragments arranged by size:
3400 1281 214 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2968 3149 3312 2968
Size: 181 163 4674
Fragments arranged by size:
4674 181 163
BtsI GCAGTG_nn'
Cuts at: 965 1742 2261 965
Size: 777 519 3722
Fragments arranged by size:
3722 777 519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1535 1570 1950 1985 1535
Size: 35 380 35 4568
Fragments arranged by size:
4568 380 35 35
DraI TTT'AAA
Cuts at: 2017 2017
Size: 5018
DrdI GACnn_nn'nnGTC
Cuts at: 2724 3582 2724
Size: 858 4160
EaeI y'GGCC_r
Cuts at: 2603 2777 3168 3195 4540 2603
Size: 174 391 27 1345 3081
Fragments arranged by size:
3081 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2603 2603
Size: 5018
EarI CTCTTCn'nnn_
Cuts at: 130 266 421 1106 1112 3041 3251 130
Size: 136 155 685 6 1929 210 1897
Fragments arranged by size:
1929 1897 685 210 155 136 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3226 3536 3682 4828 3226
Size: 310 146 1146 3416
Fragments arranged by size:
3416 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1532 2030 1532
Size: 498 4520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 541 566 785 829 971 1015 1296 1320
Size: 300 25 219 44 142 44 281 24
Cuts at: 1320 1441 1474 1548 2843 3275 4022 4290 241
Size: 121 33 74 1295 432 747 268 969
Fragments arranged by size:
1295 969 747 432 300 281 268 219
142 121 74 44 44 33 25 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1204 1833 1204
Size: 629 4389
EcoO109I rG'GnC_Cy
Cuts at: 120 246 1436 1448 1522 1719 1949 2157 120
Size: 126 1190 12 74 197 230 208 2981
Fragments arranged by size:
2981 1190 230 208 197 126 74 12
EcoRI G'AATT_C
Cuts at: 2022 2022
Size: 5018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 5018
FspI TGC'GCA
Cuts at: 877 2799 877
Size: 1922 3096
HaeII r_GCGC'y
Cuts at: 283 1625 2452 2700 3722 4701 283
Size: 1342 827 248 1022 979 600
Fragments arranged by size:
1342 1022 979 827 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 453 485 880 912 1359 1391 1933 1965 2860
Size: 32 395 32 447 32 542 32 895
Cuts at: 2860 2890 2892 2922 453
Size: 30 2 30 2549
Fragments arranged by size:
2549 895 542 447 395 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2057 2057
Size: 5018
HindIII A'AGCT_T
Cuts at: 797 797
Size: 5018
KasI G'GCGC_C
Cuts at: 2448 2696 2448
Size: 248 4770
KpnI G_GTAC'C
Cuts at: 1092 2038 1092
Size: 946 4072
MluI A'CGCG_T
Cuts at: 4451 4451
Size: 5018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1520 3689 3873 1520
Size: 2169 184 2665
Fragments arranged by size:
2665 2169 184
MscI TGG'CCA
Cuts at: 2779 2779
Size: 5018
MslI CAynn'nnrTG
Cuts at: 1201 3007 3134 1201
Size: 1806 127 3085
Fragments arranged by size:
3085 1806 127
MspA1I CmG'CkG
Cuts at: 300 318 426 566 666 873 1488 2129 2443
Size: 18 108 140 100 207 615 641 314
Cuts at: 2443 2803 3816 4061 300
Size: 360 1013 245 1257
Fragments arranged by size:
1257 1013 641 615 360 314 245 207
140 108 100 18
NaeI GCC'GGC
Cuts at: 506 3199 506
Size: 2693 2325
NarI GG'CG_CC
Cuts at: 2449 2697 2449
Size: 248 4770
NcoI C'CATG_G
Cuts at: 530 1934 530
Size: 1404 3614
NgoMIV G'CCGG_C
Cuts at: 504 3197 504
Size: 2693 2325
NheI G'CTAG_C
Cuts at: 1033 1033
Size: 5018
NspI r_CATG'y
Cuts at: 360 1687 2071 2571 3478 360
Size: 1327 384 500 907 1900
Fragments arranged by size:
1900 1327 907 500 384
PciI A'CATG_T
Cuts at: 3474 3474
Size: 5018
PfoI T'CCnGG_A
Cuts at: 1131 1131
Size: 5018
PmeI GTTT'AAAC
Cuts at: 2017 2017
Size: 5018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1790 1822 4157 4189 1790
Size: 32 2335 32 2619
Fragments arranged by size:
2619 2335 32 32
PpuMI rG'GwC_Cy
Cuts at: 246 1448 1522 1719 1949 246
Size: 1202 74 197 230 3315
Fragments arranged by size:
3315 1202 230 197 74
PspOMI G'GGCC_C
Cuts at: 119 1436 119
Size: 1317 3701
PstI C_TGCA'G
Cuts at: 701 872 1749 2065 2750 701
Size: 171 877 316 685 2969
Fragments arranged by size:
2969 877 685 316 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5018
PvuII CAG'CTG
Cuts at: 300 566 666 873 1488 2443 2803 300
Size: 266 100 207 615 955 360 2515
Fragments arranged by size:
2515 955 615 360 266 207 100
RsrII CG'GwC_CG
Cuts at: 3213 3213
Size: 5018
SacI G_AGCT'C
Cuts at: 1534 2032 1534
Size: 498 4520
SacII CC_GC'GG
Cuts at: 319 319
Size: 5018
SalI G'TCGA_C
Cuts at: 2055 2055
Size: 5018
SanDI GG'GwC_CC
Cuts at: 1448 1448
Size: 5018
SapI GCTCTTCn'nnn_
Cuts at: 3041 3251 3041
Size: 210 4808
SbfI CC_TGCA'GG
Cuts at: 2065 2065
Size: 5018
ScaI AGT'ACT
Cuts at: 4535 4535
Size: 5018
SfcI C'TryA_G
Cuts at: 33 697 868 1138 1668 1745 2061 2746 3739
Size: 664 171 270 530 77 316 685 993
Cuts at: 3739 3930 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 664 530 316 270 191
171 77
SfoI GGC'GCC
Cuts at: 2450 2698 2450
Size: 248 4770
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
SmaI CCC'GGG
Cuts at: 1223 2040 1223
Size: 817 4201
SmlI C'TyrA_G
Cuts at: 2173 3088 3580 3842 2173
Size: 915 492 262 3349
Fragments arranged by size:
3349 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5018
SphI G_CATG'C
Cuts at: 360 1687 2071 2571 360
Size: 1327 384 500 2807
Fragments arranged by size:
2807 1327 500 384
StuI AGG'CCT
Cuts at: 500 705 500
Size: 205 4813
StyI C'CwwG_G
Cuts at: 530 986 1041 1518 1586 1722 1763 1934 2152
Size: 456 55 477 68 136 41 171 218
Cuts at: 2152 2506 530
Size: 354 3042
Fragments arranged by size:
3042 477 456 354 218 171 136 68
55 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 426 523 1358 1947 1978 2601 2734 3193 3402
Size: 97 835 589 31 623 133 459 209
Cuts at: 3402 426
Size: 2042
Fragments arranged by size:
2042 835 623 589 459 209 133 97
31
TatI w'GTAC_w
Cuts at: 4533 4533
Size: 5018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 944 976 1318 1333 1350 1365 4535 4567 944
Size: 32 342 15 17 15 3170 32 1395
Fragments arranged by size:
3170 1395 342 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 2815 2815
Size: 5018
XbaI T'CTAG_A
Cuts at: 2049 2049
Size: 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1320 1997 2513 1295
Size: 25 677 516 3800
Fragments arranged by size:
3800 677 516 25
XmaI C'CCGG_G
Cuts at: 1221 2038 1221
Size: 817 4201
XmnI GAAnn'nnTTC
Cuts at: 226 448 226
Size: 222 4796
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI BaeI
BbsI BfrBI BglI BmgBI BsiWI BsmI BsrGI BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI