Restriction map for
pF1KB7239
(Circular) (Six-base) MAPSORT of: pF1KB7239.seq Check: 9700 from: 1 to: 3785
pF1KB7239 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 253 361 253
Size: 108 3677
AccI GT'mk_AC
Cuts at: 178 285 823 178
Size: 107 538 3140
Fragments arranged by size:
3140 538 107
Acc65I G'GTAC_C
Cuts at: 206 801 206
Size: 595 3190
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1610 2042 2789 3057 508
Size: 1102 432 747 268 1236
Fragments arranged by size:
1236 1102 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 240 240
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 672 1090 2657 672
Size: 418 1567 1800
Fragments arranged by size:
1800 1567 418
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 789 3138 3394 789
Size: 2349 256 1180
Fragments arranged by size:
2349 1180 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 805 805
Size: 3785
BamHI G'GATC_C
Cuts at: 512 810 512
Size: 298 3487
BanI G'GyrC_C
Cuts at: 206 265 801 1215 1463 1498 206
Size: 59 536 414 248 35 2493
Fragments arranged by size:
2493 536 414 248 59 35
BanII G_rGCy'C
Cuts at: 799 1829 799
Size: 1030 2755
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 483 1300 3337 483
Size: 817 2037 931
Fragments arranged by size:
2037 931 817
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 1410 1503 2559 1410
Size: 93 1056 2636
Fragments arranged by size:
2636 1056 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 3785
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 778
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 339 743 974 1495 2173 2447 2594 3005 339
Size: 404 231 521 678 274 147 411 1119
Fragments arranged by size:
1119 678 521 411 404 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 3755
BseYI C'CCAG_C
Cuts at: 717 1210 1968 2545 3041 717
Size: 493 758 577 496 1461
Fragments arranged by size:
1461 758 577 496 493
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 3785
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
BsmI GAATG_Cn'
Cuts at: 298 298
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 799 1410 1503 1577 1767 1829 2559 799
Size: 611 93 74 190 62 730 2025
Fragments arranged by size:
2025 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 743 3005 743
Size: 2262 1523
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 253 361 680 835 1049 1351 1732 253
Size: 108 319 155 214 302 381 2306
Fragments arranged by size:
2306 381 319 302 214 155 108
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 734 1697 734
Size: 963 2822
BsrFI r'CCGG_y
Cuts at: 1783 1964 2173 1783
Size: 181 209 3395
Fragments arranged by size:
3395 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstXI CCAn_nnnn'nTGG
Cuts at: 276 747 276
Size: 471 3314
BstYI r'GATC_y
Cuts at: 1 483 512 810 1277 1300 1635 1881 2893
Size: 482 29 298 467 23 335 246 1012
Cuts at: 2893 2979 2991 3337 1
Size: 86 12 346 449
Fragments arranged by size:
1012 482 467 449 346 335 298 246
86 29 23 12
BstZ17I GTA'TAC
Cuts at: 286 286
Size: 3785
BtgI C'CryG_G
Cuts at: 659 659
Size: 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
ClaI AT'CG_AT
Cuts at: 539 539
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 1370 1544 1935 1962 3307 1370
Size: 174 391 27 1345 1848
Fragments arranged by size:
1848 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 694 1993 2303 2449 3595 694
Size: 1299 310 146 1146 884
Fragments arranged by size:
1299 1146 884 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 778 1610 2042 2789 3057 508
Size: 270 832 432 747 268 1236
Fragments arranged by size:
1236 832 747 432 270 268
EcoNI CCTnn'n_nnAGG
Cuts at: 520 520
Size: 3785
EcoO109I rG'GnC_Cy
Cuts at: 198 241 924 198
Size: 43 683 3059
Fragments arranged by size:
3059 683 43
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 531 588 1219 1467 2489 3468 531
Size: 57 631 248 1022 979 848
Fragments arranged by size:
1022 979 848 631 248 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 1627 1657 1659 1689 640
Size: 32 955 30 2 30 2736
Fragments arranged by size:
2736 955 32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 210 805 210
Size: 595 3190
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 222 745 1774 1901 222
Size: 523 1029 127 2106
Fragments arranged by size:
2106 1029 523 127
MspA1I CmG'CkG
Cuts at: 717 896 1210 1570 2583 2828 717
Size: 179 314 360 1013 245 1674
Fragments arranged by size:
1674 1013 360 314 245 179
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 221 838 1338 2245 221
Size: 617 500 907 1761
Fragments arranged by size:
1761 907 617 500
PasI CC'CwG_GG
Cuts at: 522 522
Size: 3785
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 198 241 198
Size: 43 3742
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 583 615 583
Size: 32 3753
PstI C_TGCA'G
Cuts at: 264 832 1517 264
Size: 568 685 2532
Fragments arranged by size:
2532 685 568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 648 1980 648
Size: 1332 2453
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 260 828 1513 2506 2697 33
Size: 227 568 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 227 191
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 940 1855 2347 2609 940
Size: 915 492 262 2116
Fragments arranged by size:
2116 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 221 838 1338 221
Size: 617 500 2668
Fragments arranged by size:
2668 617 500
StyI C'CwwG_G
Cuts at: 234 275 919 1273 234
Size: 41 644 354 2746
Fragments arranged by size:
2746 644 354 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 422 535 592 1368 1501 1960 2169 422
Size: 113 57 776 133 459 209 2038
Fragments arranged by size:
2038 776 459 209 133 113 57
TatI w'GTAC_w
Cuts at: 557 569 3300 557
Size: 12 2731 1042
Fragments arranged by size:
2731 1042 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI